methylation sulfation and isomerization of chlorogenic acid metabolites
báo cáo hóa học: " Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry" doc
... internal of 13,14-dihydro-15-keto-PGD2 with a) Calibration curve standard and b) without internal standa) Calibration curve of 13,14-dihydro-15-keto-PGD2 with PGE2-d4 as internal standard and b) ... b) without internal standard Figure the corresponded product the deuterated standards with Standard chromatogram of ion scans Standard chromatogram of the deuterated standards with the corresponded ... Leukotrienes LTB4 and LTE4as analytical standards and the labelled [2H4] LTB4 and [2H4] PGE2 as internal standards were purchased from Cayman Chemicals Company (Michigan, USA) All analytical standards...
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In vitro and in vivo studies into the antidiabetic and antilipidemic effects of chlorogenic acid
... phosphorylations of AMPK and ACC and expression of CAMKKβ in liver and skeletal muscles of Leprdb/db mice 107 3.3.3 Inhibition and knockdown of AMPK abolished CGA-inhibited gluconeogenesis and ... IN VITRO AND IN VIVO STUDIES ON THE ANTIDIABETIC AND ANTILIPIDEMIC EFFECTS OF CHLOROGENIC ACID ONG KHANG WEI [BSc Biomedical Science (Hons.)] A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... biological activities viii Besides VA, coffee also contains high levels of chlorogenic acid (CGA) Regular consumption of coffee has been associated with a lower risk of Type diabetes mellitus (T2DM)...
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Structure, Properties, and Preparation Of Boronic Acid Derivatives Overview of Their Reactions and Applications
... the presence of two hydroxyl groups, the acidic character of most boronic acids is not that of a Brønsted acid (i.e., oxyacid) (Equation 1, Figure 1.6), but usually that of a Lewis acid (Equation ... explain the popularity of boronic esters as surrogates of boronic acids (Section 1.2.3.2) The Lewis acidity of boronic acids and the hydrogen bond donating capability of their hydroxyl groups ... the new status of boronic acids as an important class of compounds in chemistry and medicine This chapter describes the structural and physicochemical properties of boronic acids and their many...
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Pharmacokinetics and pharmacogenetics of mycophenolic acid in asian renal transplant patients in singapore
... the quantification of the individual metabolites of MPA and free concentrations of MPA and its metabolites, when required Based on a report of a roundtable discussion on TDM of MPA, the desirable ... data of MPA and MPAG 101 Table 4.4 Apparent clearance values and elimination half-lives of MPA and MPAG, and apparent volume of distribution for MPA obtained based on the five-compartment drug and ... showing inter-individual variability of total and free 152 MPA and MPAG AUCss, 0-12 before and after dose normalization, and MPA and MPAG CLoral and CLu before and after normalization by TBW, in...
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báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway" pptx
... simplified diagram of enzymes and major products in the synthesis of chlorogenic acid in plants A simplified diagram of enzymes and major products in the synthesis of chlorogenic acid in plants The ... p-coumaroylquinic acid C3’H C3’H caffeoylquinic acid (e.g .chlorogenic acid) Unknown enzyme caffeoylshikimic acid HCT/HQT HCT Shikimic acid Quinic acid caffeoyl-CoA dicaffeoylquinic acids lignin Figure ... silencing of the HQT gene in tobacco and tomato results in a 98% Phenylalanine PAL Cinnamic acid C4H p-coumaric acid 4CL p-coumaroyl-CoA Quinic acid Shikimic acid HCT HCT/HQT p-coumaroylshikimic acid...
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Interactions of Bioactive Plant Metabolites - Synergism, Antagonism, and Additivity
... replicates and divided by average responses of in-tray control wells – and (2) taking the log of a function of the scaled data Each of these steps can have detrimental effects on the precision of parameter ... portions of this chapter to refer to both approved drugs and bioactive plant compounds The binding of a drug to a protein may affect the function of that protein through a number of 10 Interactions of ... binding of EGF to EGFR and the subsequent activation of EGFR by altering membrane organization related to lipid rafts As a last example, omega-3 fatty acids (EPA, eicosapentaenoic acid, and DHA,...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... O-acetylsialic acid and agglutination ability of the De-O-acetylated preparation of glycoproteins De-Oacetylation of glycoproteins was performed following the procedures of Sarris and Palade [30] and Schauer ... N-glycolylneuraminic acid, N-acetyl 9-O-acetylneuraminic acid and, 8,9-di-O-acetylneuraminic acid [6] and proved to be a potent inhibitor On the other hand PSM, which contains 90% (v/v) N-glycolylneuraninic acid, ... microtiter well and mixed The values of the HAI titer were determined after 60 of incubation and expressed as the highest dilution of sugars that inhibited the agglutination of erythrocytes Mannose,...
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Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf
... helices AV and BV at the surface of domain V Gly621 and Gly617 are in the area of contact with the 1095 and 2473 regions of 23S RNA The two helices are facing the ribosome, and the four-stranded b-sheet ... type and various mutants, domains III, IV and V display a movement relative to domains I and II, resulting in a shift of the ˚ tip of domain IV of up to A [14,16] The ribosomebound structure of ... 666 and 670, within one b-strand of domain V, are also important for interdomain interactions The N-terminal part of the strand interacts with the N-terminal part of helix CG in domain G, and...
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Báo cáo khoa học: ATP-dependent ligases in trypanothione biosynthesis – kinetics of catalysis and inhibition by phosphinic acid pseudopeptides doc
... phosphonic and phosphinic acid derivatives of GSH as moderate Kinetics and inhibition of ATP-dependent ligases inhibitors of CfGspS [38] The most active of these was a phosphonic analogue of GSH ... auto-oxidation of NADPH and hydrolysis of ATP in the coupling system, the net rates of endogenous ATPase activity ($ 0.01% of kcat) in the presence and absence of inhibitor are 1.4 ( 0.9) ã 10)3 and 3.0 ... insertion of 17 amino acids in the amidase domain and two in the synthetase domain (one of 14 amino acids and the Fig Conservation of key functional residues identied for EcGspS in CfGspS and TryS...
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Báo cáo khoa học: Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) pptx
... Sequence alignment and amino acid sequence identity of zebrafish Fabp6 and FABP6s from various species, and paralogs of other zebrafish Fabps and human FABPs The deduced amino acid sequence of the zebrafish ... membranes from the detergent effects of excess fatty acids by sequestering them, and (d) modulation of cell growth and differentiation by transport of fatty acids to the nucleus where they activate ... for a polypeptide of 131 amino acids, with a molecular mass of 14 406 Da and an isoelectric point of 6.59 With the exception of some Fabp10s, which have an isoelectric point of 8.8–9.0, all other...
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
... amount of D-amino acids produced was determined by the Chirobiotic T HPLC method and the appropriate D-amino acids were used as the standards RESULTS Identification and phylogenetic analysis of the ... Influences of temperature and pH on enzyme activity For the determination of the optimal temperature of the enzyme, the reaction was carried out at 25, 30, 35, 40, 45, 50, 55, 60, 65 and 70 °C and the ... Table Comparison of the amino acid sequence similarity of putative N-D-AAases from V paradoxus and other species The gene accession number and the strains are the same as in Fig Amino acid Accession...
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Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
... Heparin cofactor II: N-glycans and sulfation (Eur J Biochem 269) 981 Fig MALDI-TOF-MS tryptic peptide fingerprints of natural (A) and recombinant (B) HCII Amino -acid sequence, calculated, and experimentally ... the accurate and efficient cleavage of the 19-amino -acid signal peptide in CHO cells [5,28] Characterization of the tryptic peptides of serum-derived and recombinant HCII Natural and recombinant ... reduction and permethylation of the total glycan mixtures, the MALDI-TOF spectra depicted in Fig revealed the presence of a mainly diantennary disialylated structure and approximately 15% of triantennary...
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Báo cáo Y học: Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt
... independent regulation of these two genes and of the accumulation of bryonolic acid and phytosterols It is noteworthy that the changes in their mRNA levels correlated with the accumulation of the respective ... isomultiflorenol synthase and cycloartenol synthase mRNA in cultured Luffa cells (A) Time course of accumulation of bryonolic acid and total sterol in cultured Luffa cells Mean of three replicates ... the bryonolic acid level increased during the late growth phase only [16,17] The level of isomultiflorenol synthase mRNA was high at days 16 and 20 and low at days and The induction of this mRNA...
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Báo cáo khoa học: Plant oxylipins: role of jasmonic acid during programmed cell death, defence and leaf senescence doc
... to OPDA and JA synthesis [34] By contrast, fractions of the unsaturated membrane fatty acid a-linolenic acid and a-linoleic acid are converted randomly and nonenzymatically to a variety of products ... 4668 Fig Role of EDS1 ⁄ PAD4 ⁄ SAG101 and BON1 ⁄ BAP1 + in cell death and plant pathogen resistance Whereas EDS1, PAD4 and SAG101 are positive regulators of cell death, BON1, BAP1 and BAP2 operate ... to Chlide and the block in 5-aminolevulinic acid synthesis released [22] Feedback control of 5-aminolevulinic acid synthesis has been attributed to heme and Pchlide [23,24] A mutant of Arabidopsis...
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Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identification of amino acid residues critical for eIF5A activity and hypusine modification doc
... to 30% of the initial rate of UBHY-R, whereas the rates of synthesis of DNA and RNA were 70% and 60% of the initial values, respectively These data suggest that the primary effect of eIF5A ... b-sheet core structures of both the N-terminal and C-terminal domains of eIF5A (Fig 1, amino acids 17–82 and 85–146) are necessary for the biological functions of eIF5A The loss of eIF5A function for ... Analysis of the position of both amino acids in the human eIF5A-1 model, Leu91 and Leu101, shows that they are localized at the hydrophobic core of the b-barrel (Fig 1) Substitution of either of the...
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Báo cáo khoa học: Fatty acid composition of chylomicron remnant-like particles influences their uptake and induction of lipid accumulation in macrophages pot
... degree of unsaturation of dietary fatty acids and the development of atherosclerosis (review) J Nutr Biochem 14, 182–195 Lambert MS, Botham KM & Mayes PA (1996) Modification of the composition of ... at 13 h (P < 0.01) and 24 h (P < 0.01) Corn and fish CRLPs were taken up at very similar rates, and there were also no significant differences in the rates of uptake of palm and olive CRLPs Fig ... enriched in SFAs, MUFAs, n)6 PUFAs and n)3 PUFAs [23], as well as containing a range of other fatty acids, and these enrichments influence the uptake and metabolism of the particles by the liver [24–26,31]...
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Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc
... stabilization of labile drugs, control of volatility and sublimation, physical isolation of incompatible compounds, long-term protection of color, odor and flavor, and suppression of hemolysis and the ... different conditions of pH, buffer and temperature In addition, NMR and DLS analyses demonstrated the characteristic properties of GA Our results of the molecular interaction between GA and c-CD can ... ITC profiles of the GA binding to CDs A 2.2 mM solution of GA was injected 16 times in increments of 10 lL into the experimental buffer (A), and a 0.1 mM solution of c-CD (B), a-CD (C), and b-CD...
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synthesis and study of acid catalyst 30% wo3 sno2
... in heteropolyacids, as have been found for 437 the catalyst WO 3rZrO w2,3x Such a structure may be responsible for the high acidity characteristic of heteropolyacids The acidity of the catalyst ... because of brown-grey coloring So we studied its acidity by thermoprogrammed desorption of pyridine adsorbed from benzene solution There was the band at 1540 cmy1 in the IR-spectrum of adsorbed ... Bronsted-type acid centres, ¨ and Lewis centres were not found Under heating at the rate 108rmin, Py was removed up to 5208C Two maxima at 4158C and 4808C appeared in the DTA curve The amount of acid...
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... and 1% (v/v) acetic acid, destained for 10 in 50% (v/v) methanol and 10% (v/v) acetic acid, rinsed (5 · min) with 25 mL of Milli-Q H2O, and airdried In situ trypsin digestion and separation of ... analyses using 12% (w/v) separating gels of purified SAP1 and SAP2 (B) Lanes and of the denaturing SDS gel contain lg of the final preparations of SAP1 and SAP2, respectively The gel was stained ... R-250 The migration of various Mr standards is shown on the left TD, tracking dye front (C) Lanes and of this nondenaturing gel contain 0.5 lg of the final preparation of SAP1 and SAP2, respectively...
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Báo cáo Y học: Amidolytic activity of prostatic acid phosphatase on human semenogelins and semenogelin-derived synthetic substrates docx
... and activation of acid phosphatase in seminal ¯uid Int J Androl 4, 372±378 10 Meng, T.C & Lin, M.F (1998) Tyrosine phosphorylation of c-ErbB-2 is regulated by the cellular form of prostatic acid ... same experimental conditions Conversely, acid phosphatase activity was measured in Synthesis of ¯uorogenic substrates the presence of NaF and of those of inhibitors that alter amidolytic activity ... using ENZFITTER software (Biosoft) Excitation and emission wavelengths were 320 nm and 420 nm for experiments with intramolecularly quenched ¯uorogenic substrates The system was standardized using...
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