... with the technical aspects of data import and management, and refer the interested reader to the documentation ofthe software package prada for a thorough discussion of these [19] Selection of ... used forthe different assays, each specifically measuring the phenotype of interest In the case of cell proliferation, the antibody detected the incorporation ofthe thymidine analog BrdU into the ... obtained either adaptively, for each well, or more globally, forthe whole plate Because ofthe potential problems with over-fitting in the adaptive approach, we choose the latter, making use of the...
... β-CD-NH2 and the backward movement ofthe neutral sample partitioned in the micelles of sodium dodecyl sulfate (SDS) Stability ofthe inclusion complexes formed between the carrier and the solute ... displacer plug was introduced before sample injection to reduce the length ofthe concentrated sample zone and increase the peak height by slowing down the forward movement ofthe neutral sample associated ... With the high separation efficiency, high resolution and portability, CE has been proven a powerful method forthe studies of many groups of compounds The improvement of sensitivity and the miniaturization...
... Where, Δp: the pressure difference; r: the inner diameter ofthe capillary; 20 t: the injection time; η: the viscosity ofthe buffer; L: the total length ofthe capillary In the case of all hydrodynamic ... macromolecules The transport ofthe solutes through the 15 capillary is based on the charge ofthe macromolecules, but the separation is dependent on the molecular size Besides the gel forming medium, other ... than the EOF as their velocity direction is the same as that ofthe EOF, so very rapid analysis times are therefore achieved with positively charged compounds The neutral compounds move at the...
... of 95 ml) The A280 to A260 ratio ofthe UV-absorbing peak indicated it was composed mainly of nucleic acid In the profile ofthe NaOH-extracted polymer (B), the elution volume (80 ml) of saccharide ... compared to the other methodsThe SDS-extracted polymer included a small amount of material other than protein Furthermore, the steaming- and washing-extracted polymers contained large amounts of phosphorus ... buffer at a flow rate of ml/h and ml fractions were collected The void volume ofthe column was determined by the elution of Blue Dextran (Pharmacia) Other analyses - 147 - Journal of Water and Environment...
... assessment ofthe trajectory of interconversion rates as a function of time of cold exposure, (b) comparison ofthe magnitudes ofthe various interconversion rates, and (c) lineup ofthe accessions ... photosynthesis, gas exchange of plants was measured by infrared gas analysis Measurements were performed during the first h 508 ofthe light phase, representing the time period of photosynthetic ... terms ofthe absolute values but also in the progression ofthe acclimation process This is an important outcome, as it allows estimation ofthe impact of different metabolic responses on the improvement...
... used, rather than pure oxygen, avoiding the use of highly flammable or explosive gases in the laboratory The oxygen is added either in the spray chamber or using a T-connector before the torch ... suffer from the formation of new “cluster” species observed with the use of more reactive gases such as ammonia The performance of CRC based ICP-MS instruments cannot, however, match the detection ... analysisofthe organic solvent is the preferred method of analysis, either untreated or simply diluted in a suitable solvent Direct organic solvent analysis demands some...
... scrutinized the German version of this text Prof Nirschl suggested the idea of this book Prof Reimert organized the continuation ofthe research unit after the DFG funding as well as Prof Kasper, Prof ... derivation due to the time-dependence ofthe unit vectors Using the product rule forthe derivation and the fact that the time derivative ofthe unit vector is the cross product of !rf with this ... the scaling ofthe argument on index k ofthe measured data in reciprocal space As the sum is finite, it only represents an approximation ofthe delta function The position ofthe origin and the...
... program power system analysis These static load models are adequate for some types of power system dynamic analysis but not for others There remains a necessity forthe development of accurate dynamic ... data 2.6 APPROACHES FOR STABILITY ANALYSISThe complete model forthe stability analysisof a typical power system is a set of DAEs numbering on the order of thousands or tens of thousands At present, ... integration ofthe fault-on system In the second step, the direct methods directly determine, without numerical integration ofthe postfault system, whether the initial state ofthe postfault...
... knowledge related to the mechanics of human movement The work at the University of California formed the basis for many ofthe fundamental techniques currently used forthe study of human locomotion ... patterns of locomotion requires new methods that would limit the risk of producing artifact due to markers or the constraints ofthe testing methodsFor example, the constraints ofthe laboratory ... Journal of NeuroEngineering and Rehabilitation 2006, 3:6 the capture and analysisof human movement For example, the Weber brothers (1836) reported one ofthe first quantitative studies ofthe temporal...
... on the percentage of fully brown beans in the cut test 6: The effect of spreading of beans for two hours, prior to placement in boxes, on fermentation temperatures 7: The effect of spreading of ... reveals the presence of certain defects which may cause off-flavours and indicates the degree of fermentation ofthe beans which has a bearing on the flavour and quality ofthe beans The International ... case of larger quantities of beans e.g 250kg, partitioned boxes (Illustration 13) are used In this case, turning of beans is performed by shovelling them from one side ofthe partition to the other...
... nonconductive, they severely distort the signal in the detector; therefore, many systems remove the bubbles before the optical light path However, if the system removes the bubbles at any point within the ... depends on the size and range ofthe scales With practice, the recommended nomographs can be read with a precision of 1% However, the overall bias ofthe results depends on the bias ofthe analytical ... data applied to the nomographs and the validity ofthe theoretical equations and the numerical constants on which the nomographs are based An approximate check ofthe bias ofthe calculations...
... Federation Standard Methodsforthe Examination of Water and Wastewater Organism Plate 10900 E Selected Taxonomic References The most useful references forthe nonspecialist are listed below These references ... D.J REISH & J.W ANDERSON, eds 1994 Ecology ofthe Southern California Bight Univ California Press, Berkeley REISH, D.J 1995 Marine Life of Southern California, 2nd ed Kendall/ Hunt Publ Co., Dubuque, ... Water Environment Federation Standard Methodsforthe Examination of Water and Wastewater Algae General TAYLOR, W.R 1957 Marine Algae ofthe Northeastern Coast of North America, 2nd ed Univ Michigan...
... Comparison of Diagnostic Methodsforthe Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the ... normal PCR reaction of 50 µl volume (25µl of master mix, Comparison of Diagnostic Methodsforthe Detection of Parasites in Fish 2µl of each primer, or µl of template and 18 or 20 µl of water) - Polymerase ... impact in many areas of parasitology, including the identification and classification of parasites, the diagnosis of infections, the epidemiology of parasites, theanalysisof population genetic...
... volume of PBS The homogenized egg yolk was then mixed with two volumes of chloroform and incubated for hrs at room temperature After this incubation, the supernatant was collected To compare the ... respectively, after the first injection (Fig and 2) The titers ofthe vaccinated groups were three times higher than those ofthe control group Antibody profiles against OMP and DNT of B bronchiseptica ... Co., Germany) for 30 seconds After centrifugation ofthe sonicated bacterial culture broth at 8,000 rpm for 30 min, the supernatant was centrifuged at 20,000 rpm for hr at 40C and the pellet was...
... reflectance in the visible yet reduced reflectance in the NIR and SWIR regions ofthe spectrum Analysis of vegetation spectra 519 Figure Summary of F-ratio tests of significance ofthe variance ofthe ... differences in these factors are also conjectured forthe eucalypt foliage These a priori expectations were tested with the null hypothesis of no treatment viz no species effect With the assumption of a ... colinearity, the approach should also reduce the dimensions ofthe data with a minimum of information loss It must accomplish this reduction without losing the ability to interpret the results Transforming...
... Morphological analysis In the second case ofthe figure, the heterozygote deficit (f) must be considered The frequency ofthe phenotype A+ is the sum ofthe frequency ofthe genotype P : +/+ All ofthe ... means ofthe comparison ofthe RAPD marker frequencies of a subsampling of these progenies and ofthe adult population Analysisofthe morphological characters MATERIALS AND METHODS Sampling The ... classified in the space ofthe other species over the analyses Molecular analyses The allele frequencies ofthe RAPD loci in the adult trees ofthe stand, calculated according to the two hypotheses:...
... functioning of each method was not deteriorated by the other The relationship obtained with the comparison ofthe two methods over the whole period (n 589) is presented in figure The slope ofthe regression ... transpiration The combination ofthe two methods seems possible in the same experiment The heat balance forthe small trunks, and transpiration calculation for small periods and Granier’s method forthe ... Pairs of thermocouples inserted above and below the heater allow the measurement ofthe conduction flux (Qv) The radial outward flow (Qr) is calculated from thermopile meathermopile was composed of...
... describing the induction of MMP-1 and MMP-3 in cartilage and SFB by TNFα and/or IL-1β [42,43] Therefore, they support the validity ofthe new model fortheanalysisofthe mechanisms of cartilage ... established the modified, present form ofthe model, performed the real-time PCR, the immunohistochemistry and the respective data analyses and wrote the manuscript D Pohlers performed some oftheexperiments ... higher amount of damage than the respective monocultures Collagen synthesis In view of an amplification ofthe potential net loss of collagen from the cartilage matrix, the synthesis of collagen...