methods for analysis of dna chip experiments
Development and application of novel capillary electrophoresis techniques for analysis of DNA fragments and organic pollutants
Ngày tải lên :
14/09/2015, 11:40
... requirement for analysis of different analytes Table 1.1 summarizes the methods to control the EOF [5, 6] The analytical parameters for CE can be described in similar terms as those for high performance ... the CE applications in DNA analysis and monitoring of pollutants are of significant importance 1.3.1 CE Application in DNA Analysis DNA separation by CE has been the subject of intensive research ... Electrophoresis of ΦX174 DNA under Different Applied Voltage 66 XX List of Figures Figure 2.8 Demonstration of Separation of ΦX174 DNA by CE-PGD and CE-UV 67 Figure 3.1 Design of CCD...
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Báo cáo hóa học: "Multicriteria Gene Screening for Analysis of Differential Expression with DNA Microarrays" potx
Ngày tải lên :
23/06/2014, 01:20
... impeded their use for practical experiments First, is the need for improved relevance of the fitness criterion to the scientific objectives of the experiment It is often difficult for an experimenter ... algorithms is indicated in the first two columns of Table The maximum and median of the FDR P values CONCLUSION Signal processing for analysis of DNA microarrays for gene expression profiling is a rapidly ... paired-t test of hypotheses (1) with fcmin = at a FDR level of 20% The second stage of the “Two-stage FDR-CI” algorithm selects Ᏻ2 as a subset of Ᏻ1 at the prescribed FDR-CI level of 20% Stage of the...
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Báo cáo sinh học: "Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection" pptx
Ngày tải lên :
14/08/2014, 19:22
... per plate, while descriptions of the methods/ outcomes for the 96 conditions and easy-to-use protocols for the evaluation of the results are lacking We therefore performed several simple, duplicate ... single-cuvette format for a variety of mammalian cell types, and recently for a 96well shuttle system However, the shuttle system must be optimized for each cell type, which involves the screening of up ... 96-well nucleofection plate was loaded with 20 μL of one of the three nucleofection solutions (approximately 275,000 cells) and the plate was loaded into the Amaxa 96-well Shuttle for nucleofection...
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Tài liệu Báo cáo khoa học: Application of a fluorescent cobalamin analogue for analysis of the binding kinetics A study employing recombinant human transcobalamin and intrinsic factor pdf
Ngày tải lên :
19/02/2014, 05:20
... excess of 0.2 m phosphate buffer pH 7.5 Synthesis of the uorescent Cbl analogue CBC-244 Activation of the 5Â hydroxyl group in the a-ribofuranoside moiety of CNCbl was performed with help of 1,1Â-dicarbonyl-di-(1,2,4-triazole) ... Spectral properties of CBC The coefcient of molar absorbance for rhodamine moiety of CBC was estimated as e527 ẳ 90 000 m)1ặcm)1 In the below experiments we used concentrations of CBC Ê lm, where ... below Competitive binding of CBC and Cbl, calculation of k+ We have tested the application of the uorescent analogue CBC as a tool for investigation of the binding kinetics of nonuorescent ligands...
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Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx
Ngày tải lên :
06/03/2014, 09:22
... holder) for The excitation wavelength was set at 295 nm for selective excitation of Trp residues, or at 320 nm for excitation of essDNA For analysis of the quenching of Trp fluorescence, Mhr1– DNA ... intensity of the essDNA–Mhr1 complexes For analysis of Mg2+ dependency, Mhr1–essDNA complexes were formed by addition of Mhr1 to 13 lm essDNA in 25 mm Tris ⁄ HCl (pH 7.5) in the presence of 10 mm ... of Mg2+ Circular ssDNA of / X174 (10 lM) or circular dsDNA of pUC119 (5 lM) was incubated with the Mhr1 mutants (0.2, 0.4, 0.6, 0.8 and 1.0 lM for ssDNA; 0.1, 0.2, 0.3, 0.4 and 0.6 lM for dsDNA)...
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Pseudo Velocity Shock Spectrum Rules For Analysis Of Mechanical Shock pdf
Ngày tải lên :
09/03/2014, 00:20
... HIGH FREQUENCY LIMIT OF THE PLATEAU OF THE UNDAMPED PVSS OF THE SIMPLE SHOCKS OF THE HIGH PV REGION IS SET BY THE MAXIMUM ACCELERATION OF THE SHOCK The Low Frequency Asymptote of a Zero Mean Shock ... relationship with four sets of lines, log spaced: vertical for frequency, horizontal for velocity, down and to the right for acceleration, and down and to the left for deflection Zero Mean Simple ... displacement line of about 50 inches Figure 4, our PVSS on 4CP, for that hard drive non operational shock, shows a lot of information We’ll talk more about this later, but for now you see a peak...
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An application of GIS and Remote Sensing for Analysis of Agricultural Development-Induced Changes in Land Use: A case study in Lao PDR pdf
Ngày tải lên :
17/03/2014, 11:20
... linkage and analysis of such data, in particular for detection, interpretation, area calculation, monitoring and future estimating Therefore, this study applied GIS and remote sensing for analysis ... way of land use system • The forest area was destroyed by increasingly shifting cultivation and rubber plantation areas • Lack of an appropriate tool for decision support system in terms of land ... with registered of image 1999 (image to image) RMC= 0.14 pixels Change pixel 30m of image 1999 to 25m of image 2004 NDVI compositing utility – NDVI is useful for identifying of the green leaf...
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SYSTEMATIC METHOD FOR ANALYSIS OF PERFORMANCE LOSS WHEN USING SIMPLIFIED MPC FORMULATIONS pptx
Ngày tải lên :
31/03/2014, 22:20
... the best way of simplification for our example of plant is found, which turns out to be delta input blocking Keywords: analysis of MPC, simplified MPC formulations, analysis of MPC performance ABSTRAKT ... SLOVAK UNIVERSITY OF TECHNOLOGY IN BRATISLAVA FACULTY OF CHEMICAL AND FOOD TECHNOLOGY SYSTEMATIC METHOD FOR ANALYSIS OF PERFORMANCE LOSS WHEN USING SIMPLIFIED MPC FORMULATIONS DIPLOMA THESIS ... Model of the Distillation Column 40 3.1.1 Disturbance Model 42 3.2 Formulation of the MPC Problems 43 3.2.1 Formulation of Problem 43 3.2.2 Formulation of Problem...
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báo cáo hóa học: " Evaluation of methods for extraction of the volitional EMG in dynamic hybrid muscle activation" pot
Ngày tải lên :
19/06/2014, 10:20
... and out of the process in various combinations * Butterworth, 4th order, with cut-offfrequency of 15 Hz ** Butterworth, 4th order, with cut-off frequency of Hz Page of 11 (page number not for citation ... Several methods were used for evaluation of the success of extraction of the volitional EMG The basic one was by visual inspection of the approximated signal which, despite not providing information ... Despite the many methods for the elimination of the ESinduced component, providing the volitional only EMG component, most of them not provide an evaluation of the accuracy of the extraction...
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Standard Methods for Examination of Water & Wastewater_1 ppt
Ngày tải lên :
19/06/2014, 16:20
... for the electrical conductivity of water The concentrations of these ions are very small At −7 25°C, for pure water, there is a concentration of × 10 mole per liter of the hydronium ion and of ... portions of the sample Each of these portions is inoculated into a set of five replicate tubes For example, for the 10-mL portion, each of five fermentation tubes is inoculated with 10-mL portions of ... coliform group and at 44.5 ± 0.2°C in the case of the fecal coliforms At the end of the incubation period of 24 ± hours, the number of colonies that developed are counted and reported as number of...
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Standard Methods for Examination of Water & Wastewater_2 ppt
Ngày tải lên :
19/06/2014, 16:20
... end of crest to gage point B = axial length of converging section C = width of downstream end of flume D = width of upstream end of flume E = depth of flume F = length of throat G = length of diverging ... Beveled edge of crest of weir FIGURE 3.2 Schematic for derivation of weir formulas is, the contraction is suppressed This happens when the weir is extended fully across the width of the channel, ... Using the equation of continuity in the form of (π D /4)V1 = (π d /4)V2, where D is the diameter of the pipe and d is diameter of the throat, the above equation may be solved for V2 to produce...
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Standard Methods for Examination of Water & Wastewater_3 potx
Ngày tải lên :
19/06/2014, 16:20
... flow radially into the side of the chamber circumscribing it, by conveying the flow forward by proper design of the impeller, and by a mix of forward and radial throw of the flow The pump that uses ... elevation of the eye of the impeller or centerline of the pump system; the second is the source tank below the eye of the impeller or centerline of the pump system The flow in flow pipes for the ... the pump At 20°C, Pv (vapor pressure of water) = 2.34 kN/m = 0.239 m of water Assume standard atmosphere of atm = 10.34 m of water Therefore, theoretical limit of pump cavitation = − (10.34 − 0.239)...
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Standard Methods for Examination of Water & Wastewater_4 potx
Ngày tải lên :
19/06/2014, 16:20
... because of formation of gases, makes solids rise resulting in inefficiency of the basin Thus, in practice, there is a practical range of values of 1.5 to 2.5 h based on the average flow for primary ... of combinations of the values of the elements of Vc[Xc] + Vu[Xc] can occur before the solids reach the critical section of the thickener This combination corresponds to the different speeds of ... treatment of sewage and the sedimentation basin used in the treatment of raw water for drinking purposes are two of the units in the physical treatment of water and wastewater that use the concept of...
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Standard Methods for Examination of Water & Wastewater_5 ppt
Ngày tải lên :
19/06/2014, 16:20
... Projected area of paddle blade Sum of projected areas of paddle blades Length of paddle blade Coefficient of drag Width of paddle blade The diameter of the impeller Diameter of vessel Distance of impeller ... length of the paddle blades of the longest compartment to that of the length of the paddle blades of the middle compartment is 2.6:2, and the ratio of the length of the paddle blades of the middle ... to each other, the rate of inflow of air corrected to its value at the surface of the vessel is then (Pi/Pa)Qi Thus, the number of bubbles n formed from a rate of inflow of air Qi is Pi Pi h ...
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Standard Methods for Examination of Water & Wastewater_6 pot
Ngày tải lên :
19/06/2014, 16:20
... 0.022(q) 2.22 hd = 0.0133(q) for uniform sand, 1.0 mm diameter 2.10 for uniform anthracite, 1.0 mm diameter 2.10 for uniform anthracite, 1.6 mm diameter 2.10 for uniform anthracite, 2.0 mm diameter ... Proportionality constant for the inertial force Proportionality constant for the shear force Length of bed Expanded bed depth Filter yield Rate of mass inflow into bed Mass of cake per unit volume of filtrate ... the temperature of filtration The value of c may be determined by performing an experiment of the particular type of sludge To determine α o , take the logarithms of both sides of Equation (7.67),...
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Standard Methods for Examination of Water & Wastewater_7 doc
Ngày tải lên :
19/06/2014, 16:20
... function of the size of the solute molecule • Percentage removal increases as the percent dissociation of the solute molecule increases The degree of dissociation of a molecule is a function of pH, ... MEMBRANE PERFORMANCE CHARACTERIZATION The performance of a given membrane may be characterized according to its product flux and purity of product Flux, which is a rate of flow per unit area of membrane, ... pressure values of various solutes Some generalizations may be made from this table For example, comparing the osmotic pressures of 1,000 mg/L of NaCl and 1,000 mg/L of Na2SO4, the former has about...
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Standard Methods for Examination of Water & Wastewater_8 docx
Ngày tải lên :
19/06/2014, 16:20
... affinity of the solute to form into a solid An example of this operation is the making of ice from liquid water The formation of snow from water vapor in the atmosphere is also a process of crystallization ... performs an experiment for the purpose of determining the value of β of a particular wastewater The [Cos,w] of the wastewater after shaking the jar thoroughly is 7.5 mg/L The temperature of the ... Therefore, 7.5 β = = 0.89 Ans 8.4 Example 9.5 An environmental engineer performs an experiment for the purpose of determining the value of β of a particular wastewater in the mountains of Allegheny...
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Standard Methods for Examination of Water & Wastewater_9 potx
Ngày tải lên :
19/06/2014, 16:20
... removed in the form of CaCO3 This is the reason for the use of the second chemical known as soda ash for the removal of the noncarbonate hardness of calcium Thus, another set of chemical reactions ... the number of reference species is moles of positive charges for each of these reactions Thus, the equivalent mass of calcium is Ca/2 and the number of equivalents of the MCa mass of calcium ... hardness of magnesium We will first calculate the amount of lime required for the removal of the carbonate hardness of calcium Let M CaHCO be the mass of Ca ( HCO ) in the carbonate hardness of calcium...
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Standard Methods for Examination of Water & Wastewater_10 docx
Ngày tải lên :
19/06/2014, 16:20
... smallest of the Ksp’s is that for Hg2CO3 Thus, considering all of the possible candidates that we have written, Hg2CO3 is the one that will form a dead end for the carbonate equilibria; however, of ... amount of calcium carbonate that precipitates is simply the equivalent of the calcium ion that precipitates, Cappt Because the number of moles of Cappt is equal to the number of moles of the ... complex of calcium ion + CaOH Hydroxide complex of calcium ion [∆carb] Moles of carbon dioxide per unit volume of flow required to bring the pH of water to the saturation pH o CaSO Sulfate complex of...
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Standard Methods for Examination of Water & Wastewater_11 potx
Ngày tải lên :
19/06/2014, 16:20
... be used Therefore, we now derive the formulas for estimating the volume of sludge produced in coagulation treatment of water Two sources of solids are available for the production of sludge: the ... at a temperature of 25°C If the formulas for the determination of these pH’s are reviewed, they will be found to be functions of equilibrium constants By the use of the Van’t Hoff equation, values ... reason for the existence of the calcium bicarbonate; however, kgeq cannot be a reason for the existence of calcium bicarbonate, since it is a unit of measurement Therefore, if we see a unit of measurement...
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