methods and materials research paper
Tài liệu Research Methods and Statistics in Psychology ppt
... selecting methods and statistical treatments for practical work and for structured questions on research examples, but also for dealing with general issues of scientific and research methods Higher ... planning research Chanter Variables and definitions Psychological variables and constructs; operational definitions; independent and dependent variables; extraneous variables; random and constant ... ERROR and RANDOM ERROR are dealt with in research Random error (or random variable) Maybe your answers to question 1included some of the following: I the way you were feeling on the day high random...
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Social Science Research - Principles, Methods, and Practices pptx
... solving and insight generation is the central feature that distinguishes action research from all other research methods, and hence, action research is an excellent method for bridging research and ... about research methods (empirical data collection and analysis) but about the entire research process” from start to end Research method is only one phase in that research process, and possibly ... deductive research if you are not familiar with both the theory and data components of research Naturally, a complete researcher is one who is able to handle both inductive and deductive research...
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Research Paper No. 2006/150 Land Titles, Credit Markets and Wealth Distributions pot
... wealth within countries This paper attempts to partially address this void, and asks whether and how the land administration system and credit market regulations for land and real estate matter for ... Deininger and Feder (2001: 288-31) note, establishing and enforcing property rights to land and real estate is costly as plots of land must be measured, accurate records of land titles maintained and ... (2003) Land Policies for Growth and Poverty Reduction, Oxford University Press for the World Bank: New York Deininger, K., and G Feder (2001) ‘Land Institutions and Land Markets’, in B.L Gardner and...
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Báo cáo khoa học: "The GENIA project: corpus-based knowledge acquisition and information extraction from genome research papers" docx
... of EACL '99 and DNA entities was too small to train with As part of the overall project we are creating an expert-tagged corpus of MEDLINE abstracts and full papers for training and testing our ... extraction methods to automatically extract named entity properties, events and other domain-specific concepts from MEDLINE abstracts and full texts One part of this work is the construction and maintenance ... References L.D Baker and A.K McCallum 1998 Distributional clustering of words for text classification In Proceedings of the 21st Annual International ACM SIGIR Conference on Research and Development...
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Earth Materials and Health: Research Priorities for Earth Science and Public Health pptx
... Earth Materials and Health: Research Priorities for Earth Science and Public Health http://www.nap.edu/catalog/11809.html EARTH MATERIALS AND HEALTH RESEARCH PRIORITIES FOR EARTH SCIENCE AND PUBLIC ... reserved Earth Materials and Health: Research Priorities for Earth Science and Public Health http://www.nap.edu/catalog/11809.html 20 EARTH MATERIALS AND HEALTH gins and island arcs In contrast ... Earth Materials and Health: Research Priorities for Earth Science and Public Health http://www.nap.edu/catalog/11809.html 12 EARTH MATERIALS AND HEALTH BOX 1.2 Statement of Task A National Research...
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chemistry and chemical engineering research progress (chemical engineering methods and technology)
... for academics, researchers, and engineering professionals to present their research and development works that have potential for applications in several disciplines of engineering and science Contributions ... science Contributions ranged from new methods to novel applications of existing methods to gain understanding of the material and/ or structural behavior of new and advanced systems Contributions ... positions of the bands are used RESULTS AND DISCUSSION There are several regions of interest in the characteristic transmittance bands at 4000-400 cm-1 for CN and CA samples These bands occurred...
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opioid research, methods and protocols
... 70 µL stock solution I to tubes A and B and 70 µL stock solution II to C and D, 10 µL 50 mM PIPES to A and C and 10 µL substrate solution to tubes B and D, and 10 µL of mM [γ-32P]ATP/ATP to A–D ... transfer buffers, 3MM papers and three transfer trays Make fresh Transfer buffers A, B, and C Place three trays on bench and label them as A, B, and C in sequential order Cut 3MM papers to fit them ... third tray containing Transfer buffer C and neutralize for Place the membrane on a dry 3MM paper to dry the membrane Sandwich the membranes with 3MM paper and cover them with a sheet of aluminum...
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Báo cáo khoa học: "Information about studies on Hungarian forest ecosystems. Selected research-papers and future investigations of Síkfõkút long-term project" pdf
... space and time; • tolerance limits to external pounds, etc AIMS OF RESEARCHES Practically, the research goals of ’Síkfõkút Project’ can be grouped around the main problems of synecology The researches ... litter decomposition tent - - - content and energy flow Measurement of solar radiation and energy balance of the forest Energy content of trees and shrubs and of their fractions D) Energy - - E) ... landscape 4 INTERDISCIPLINARY FIELDS OF RESEARCHES - - For the realization of the listed goals, intensive researches have been going on in the selected forest and its environs since 1972, within...
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Báo cáo y học: "Comparison of different methods to obtain and store liver biopsies for molecular and histological research" pot
... immunohistochemistry and RNA and protein isolation Since these diverse intentions require different fixation and storage methods, clinicians and researchers are often faced with a multitude of different vials, and ... measurements and subsequent data analysis [8-10] However, little information is available that compares different methods of retrieval, isolation and storage of canine tissues for molecular research ... populations, and research of complex diseases profits from the fact that dogs share the human environment In addition to similarities between dogs and humans with respect to physiology, pathobiology, and...
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Cambridge.University.Press.An.Introduction.to.Law.and.Regulation.Text.and.Materials.Apr.2007.pdf
... the field and an accessible and critical introduction to the subject Morgan and Yeung set out a diverse and stimulating selection of materials and give them context with a comprehensive and critical ... Imagination Zander: Cases and Materials on the English Legal System Zander: The Law-Making Process An Introduction to Law and Regulation Text and Materials Bronwen Morgan and Karen Yeung CAMBRIDGE ... Infrastructure and Human Rights Maughan & Webb: Lawyering Skills and the Legal Process McGlynn: Families and the European Union: Law, Politics and Pluralism Moffat: Trusts Law: Text and Materials...
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Glycoprotein Methods and Protocols - P1
... a UV monitor and collect fractions using a fraction collector and subject to the appropriate carbohydrate and antibody analyses 3.6 Analysis of Mucins Methods for the detection and analysis of ... antibodies and lectins or coating methods such as the glycan detection method and enzyme-linked immunosorbent assays (ELISA) All these techniques have advantages and disadvantages Solution methods ... three principally different methods are available: solution assays such as colorimetric assays for hexose and sialic acid; membrane-based methods such as slot-blotting and staining with periodic...
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Glycoprotein Methods and Protocols - P2
... mammary gland, defined by antibodies to cytokeratins J Cell Sci 75, 17–33 Kiernan, J A (1990) Histological and Histochemical Methods: Theory and Practice, Pergamon, Oxford, UK Andrew, S M and Jasani, ... M and Milton, J D (1986) Lectin Methods and Protocols, Humana, Totowa, NJ Liener, I E., Sharon, N., and Goldstein, I J (1998) The Lectins: Properties, Functions, and Applications in Biology and ... such as Culling’s Handbook of Histopathological and Histochemical Techniques (including museum techniques) (36) or Kiernan’s Histological and Histochemical Methods: Theory and Practice (45) provide...
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Glycoprotein Methods and Protocols - P3
... core protein specificity), and lower sensitivity than slot-blot and immunoassay methods Chap- Detection and Quantitation of Mucins 47 ter (2) discusses these assays and they are mentioned here ... greater sample volume, and superior washing) For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samples should ... NJ Nagma, K., Thornton, D J., Khan, N., and Sheehan, J K (2000) Separation and identification of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T.,...
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Glycoprotein Methods and Protocols - P4
... and held together by bolts tightened by hand Millipore filter paper to act as a backing for the mucosa during sectioning and subsequent manipulation of the section 2.2 Histological Staining and ... (w/v) poly-L-lysine for and dried at room temperature Methods 3.1 Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections (see Notes 1–4) This is a rapid and simple method for ... layer will be greatly reduced and discontinuous Careful handling of the cryostat sections on the slide during fixing and staining is important because excessive washing and so on can remove mucus...
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Glycoprotein Methods and Protocols - P5
... flat materials (gels or filter paper) by autoradiography It calculates the amount of radioactivity in a band on gel or on a dot on filter paper, taking into account the area of the band/dot and ... location on 3MM Whatman filter paper and air-dry Immerse the filter paper in ice-cold 5% TCA for at least 10 Transfer paper to 5% TCA at 100°C for 10 Wash the paper two times for each in 5% TCA ... Whatman 3MM filter paper 19 Molecular weight marker: nonreduced monomeric and dimeric rat gastric mucin precursors, molecular mass 300 and 600 kDa, respectively (8) (see Chapters 20 and 21) 20 Homogenization...
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Glycoprotein Methods and Protocols - P6
... a small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer Leave to cool before pouring (hand hot) and insert well-forming comb (see ... are 1.5 mm thick and cm wide There is a compromise between band broadness and amount of sample to be loaded Larger amounts of sample tend to yield poorer quality data; i.e., the bands become more ... I., and Sheehan, J K (1996) Identification of glycoproteins on nitrocellulose membranes and gels Mol Biotechnol 5, 171–176 10 Thornton, D J., Howard, M., Devine, P L and Sheehan, J K (1995) Methods...
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Glycoprotein Methods and Protocols - P7
... ethanol, remove excess solution on filter paper, and air-dry 3.3.1.3 STAINING AND SHADOWING Dip grid in staining solution for a few seconds Wash in 95% ethanol and air-dry For generating higher contrast, ... Kadler, K E., and Chapman J A (1985) Mica sandwhich technique for preparing macromolecules for rotary shadowing J Ultrastruct Res 91, 66–76 14 Kawahara K and Tanford C (1966) Viscosity and density ... into 0.5-mL fractions, and these were analyzed for reactivity with antisera for the MUC5AC (᭹) and MUC5B (᭺) mucins The MUC5B mucin is more polydisperse than the MUC5AC mucin and has molecules of...
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Glycoprotein Methods and Protocols - P8
... support for their research on amino acid analysis through grants from the Australia Research Council, Macquarie University Research Grants, National Health and Medical Research Council, and GBC Scientific ... at least for pump and column equilibration with the initial composition of the mobile phase Figures and show typical chromatograms of the separation of amino acid standards and hydrolyzed bovine ... 114 Yan and Packer chromatography, and leads to a significantly lower recovery of all the amino acids, especially serine and threonine Strong acid, high temperature, and short time acid...
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Glycoprotein Methods and Protocols - P9
... Notes and 8) and the length of sequence obtained is dependent on the number and distribution of proline residues Proline cleaves with reduced efficiency in Edman sequencing (Fig 1, vessel 1), and ... I., Kirk, M., Barnes, S., Lowary, T L., and Hart, G W (1996) Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by β-elimination and tandem electrospray mass spectrometry Anal ... PerSeptive Biosystems and Beckman supply this product Quantitation of glycopeptides is quite difficult, and we recommend that 10% of the sample be first sequenced using the standard program prior...
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Glycoprotein Methods and Protocols - P10
... tandem repeat domain and overlapping each other by six amino acids (16), and the other used octamers overlapping each other by seven amino acids (17) Twelve antibodies were analyzed in total and ... information on antibody specificity and permits identification of significant features of an epitope that may contribute to antibody recognition and binding (see Notes 10 and 11) 1.3 Purification of ... must be pure and free from amines Several methods may be used to purify DMF (see Note 16) Piperidine 20% v/v: used for Fmoc deprotection Piperidine should be redistilled before use and made up...
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