method of analysis of the isoprostanes
Báo cáo khoa học " Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake " ppt
... As is the absorbance in the presence of the sample and H2 O2 , A0 is the absorbance of the control in the absence of the sample and H2 O2 , and A is the absorbance only in the presence of the H2 ... the change speed of absorbance of the control group in the superoxide anion generation system and B is the change speed of absorbance of the test sample Vc was used as a positive control in the ... the 4000–400 cm−1 region 2.8 Scavenging effect on hydroxyl radicals The ability of the TMP-A to scavenge hydrogen peroxide was determined according to the method of Smirnoff and Cumbes [17] The...
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Báo cáo vật lý: "Evaluation of In Vitro Antioxidant Activity of 5H-dibenz[b,f]azepine and Its Analogues" ppt
... the absent of the N–H absorption band at 3400 cm–1 and the presence of the C=O stretching band at 1600 cm–1, whereas in compounds (a) and (d), the presence of N–H stretching and the absence of ... used for the preparation of the derivatives (b–f) (Schemes and 3) The structures of the compounds were elucidated by IR, 1H NMR, mass spectroscopy and elemental analysis The IR spectra of compounds ... may enhance the stability of the nitrogen centred radical due to the electron conjugation effect The absence of N– Evaluation of In Vitro Antioxidant Activity 88 H group in the other compounds...
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Báo cáo y học: "Behavioral and antioxidant activity of a tosylbenz[g]indolamine derivative. A proposed better profile for a potential antipsychotic agent" pptx
... be a bioisostere of the hydrogen-bonding H donor properties of the phenolic OH group in dopamine agonists Based on the above, we synthesized PBIA In the present work, we tested the derivative (Figure ... hypothesized that it might be capable of penetrating the blood-brain barrier in a considerable degree Additionally, the presence of the tosyl group might shift the agonistic activity to that of ... antioxidant activity Figure The Ugo-Basile activity cage (type 7401) The Ugo-Basile activity cage (type 7401) Materials and Methods Synthesis of TPBIA Key step of the synthesis was a Mukaiyama type...
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Báo cáo y học: "Antioxidant activity of tuberosin isolated from Pueraria tuberose Linn" pot
... the initial steps of the signaling cascade of LPS induced NO production, but it is still not clear, whether it is directly inhibiting the activity of iNOS enzyme or it is suppressing the synthesis ... indirect method to measure the accumulated nitrite in the culture supernatant, which reflects the concentration of released nitric oxide The EC50 value of isolated compound (concentration of sample ... radical species, could be because of the difference in the electron potential of these free radical species [38] Further, the Fe induced lipid peroxidation in presence of ascorbic acid, is an example...
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Antioxidant activity of essential oils
... antioxidant, i.e the control, was measured daily Special care was taken to minimize the loss of free radical activity of the DPPH radical stock solution.24 Percent inhibition of the DPPH radical by the samples ... used on the day of preparation In the FRAP assay, the antioxidant efficiency of the antioxidant tested was calculated with reference to the reaction signal given by an Fe2+ solution of known ... (1 – AT/AC) ´ 100 where AC is the absorbance value of the fully oxidized control and AT is the absorbance of the test sample Determination of Oxidative Stability of Fat (RANCIMAT) A Rancimat 743...
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Evaluation of the antioxidant activity of scutellaria baicalensis and its constituents in diabetic rats
... Huey Lee Then, the author wishes to express her gratitude to Professor Bay Boon Huat of the Department of Anatomy, Yong Loo Lin School of Medicine and Associate Professor Heng Chew Kiat of the Department ... repeated the work of Von Mering and Minkowski but went a step further to show the reversal of diabetic symptoms in the dogs by giving them an extract from the pancreatic islets of Langerhans of healthy ... Brownlee USA 35 Highlighting the importance of the pancreas in the disease pathology of diabetes Discovery and the isolation of insulin for the treatment of diabetes The distinction between type...
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Nghiên cứu về hoạt tính chống oxy hóa của các cây thuộc African Antioxidant Activity of African Medicinal Plants
... mg/mL) In the hydroxyl radical scavenging assay, the activity of the leaf fraction was greater than that of the other fractions but lower than that of ascorbic acid The green fruit had the highest ... glucose [35] In the DPPH radical scavenging assay, the activity of the positive control, ascorbic acid, was the highest (200 mg/mL), followed by the leaf, the green fruit, the stem, and the ripe fruit ... followed by the leaf (167 mg/mL), the stem (267 mg/mL), and the ripe fruit (173 mg/mL) [37] Bio-guided fractionation of the methanol extract of M charantia dried gourds led to the isolation of 11 compounds...
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Báo cáo y học: "Pravastatin Provides Antioxidant Activity and Protection of Erythrocytes Loaded Primaqe"
... in the shape of erythrocytes is the spherocytes formation in the presence of primaquine These observations are supported by the work done by Prasanthi et al., in 2005 which reported that the ... changes of the RBC membrane when they are subjected to osmotic stress [32] In the present study, the susceptibility of erythrocytes to hemolysis in presence of primaquine was greater than that of control ... for the increased fragility of primaquine treated erythrocytes Furthermore, free radicals can overwhelm the capacity of antioxidants enzymes to maintain and keep up the membrane integrity [34] The...
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Báo cáo hóa học: "Effects of Pin-up Oxygen on [60]Fullerene for Enhanced Antioxidant Activity" pptx
... activity The method is based on the discoloration of the yellowish color of a b-carotene solution due to the breaking of p-conjugation by the addition of lipid peroxyl radical (LOO•) generated from the ... mixture of fullerene oxide C60On.1 The dotted line indicates the value in the absence The C60On, instead of C60O2 due to the difficulty in availability, was used to investigate the effect of the number ... clarify the reason for the significant effect of the pin-up oxygen on the antioxidant activity of C60, we calculated the energy level of LUMO and HOMO for the C60, C60O, and C60O2 as well as the energy...
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Báo cáo y học: " Antioxidant components of naturally-occurring oils exhibit marked anti-inflammatory activity in epithelial cells of the human upper respiratory system" pdf
... component of the study was carried out in the Health Effects Assessment Laboratory (HEAL) in the Department of Environmental Health Sciences of the Bloomberg School of Public Health The purpose of this ... for the week prior to, and for the duration of, the 3-week study period One subject was removed from the study after the initial nasal lavage indicated the presence of very high numbers of leukocytes ... investigate the effects of the oil preparation on the global system of antioxidant genes induced within the respiratory epithelium by the Nrf2 transcription factor, representative gene targets of Nrf2...
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anthocyanin – study of extraction from basella rubra l and verifying antioxidant activity, application in identificating di sodium tetra borate in food
... the decrease of Anthocyanin concentration The reason of decreasing AA% was the decline of 14 Anthocyanin molecular through dilutions The result of antioxidant activity was shown in table 6, the ... range The color of Anthocyanin solution changed through the pH range from to 14 as shown on the figure The rising of pH from an initial point of to witnessed the stable color of Anthocyanin with ... indicator papers In the situation of Vietnamese food safety in recently, the use of food preservatives are widely beside borax and NaOH, therefore, the ability of variable identification of indicators...
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Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"
... have not yet been addressed The first question is whether the expression level for RIZ1 varies over the progression of cancer; and the second question is whether the PR domain alone possesses ... described below; and the cell death rate was examined by the trypan blue method after 24 and 48 hr of transfections, respectively Western blot of PR domain The expression of the PR domain was examined ... mediate the translocation of PR domain into the cells To ensure the presence of PR domain within the cells, we transfected HuH7 cells with a plasmid (pcDNA/HisMax/PR) expressing the PR domain The...
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ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
... nitrifying biofilm After reaching the steady-state condition, microprofiles of NH4+, NO2-, NO3-, and O2 in the biofilms were measured by use of microsensors, and the spatial distributions of in situ ... determined The relationship between the spatial organization of nitrifying bacterial populations and the in situ activity of these populations within the biofilms was discussed Microsensors For analysis ... performed Fixation and Cryosectioning of Biofilm Samples Biofilm samples were taken at regular time intervals during the periods of the biofilm development The biofilm samples were fixed with freshly...
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Photocatalytic bactericidal activity of silver-sensitized titanium dioxide on Micrococcus lylae
... around the photocatalyst and were able to penetrate the outer layer of bacteria These reactive species oxidized the membrane and broke the main permeability barrier of bacteria The slow leakage of ... on the photocatalyst surface Thus, increasing the UV intensity directly should enhance the oxidation efficiency of photocatalyst Because of the limitation on adding UV lamp in the reactor of ... main reason of cell death The cell wall destruction was only a secondary phenomenon after the loss of cell viability In the application of PCO for disinfection of water, photocatalyst is the major...
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Tài liệu Báo cáo khoa học: Multi-targeted activity of maslinic acid as an antimalarial natural compound pdf
... for the validity of the approach To the best of our knowledge, the other four proteins have never been suggested as possible targets for MA Nevertheless, there is compelling evidence of the connection ... in the processing of apicoplast protein leader sequence Inhibition of any of these activities could contribute to MA interference in the maturation of the parasite Polypharmacology of MA The ... functionality of the vacuole as a result of the accumulation of partially hydrolysed peptides, leading to the accumulation of uncleaved globin [39] The possible effect, either direct or indirect, of MA...
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Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt
... distinct functions of TorD with the folding of TorA and possible interactions of both proteins with the MGD biosynthetic apparatus [12] The concentration of GTP in the cytoplasm of an exponentially ... a result of its structural similarity to nucleotide cofactors Specifically-bound proteins are then normally eluted by the application of either an amount of cofactor or by increasing the ionic ... column, 2.3 mg of the TorDBlue protein harbouring all of the GTPase activity was recovered Further analysis of the two different TorD pools by SDS–PAGE and native PAGE suggested that the key difference...
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Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt
... initiation of apoptosis through the mitochondrial pathway The key event in the mitochondrial pathway is the release of proapoptotic factors from the mitochondrial intermembrane space into the cytosol, ... release of cytochrome c and second mitochondria-derived activator of caspase (Smac ⁄ DIABLO) allows for the formation of the apoptosome, a complex that enables the activation of caspases within the ... version of Itch together with Bid or tBid fused to green fluorescent protein (GFP) at the C-terminus We then immunoprecipitated FLAG–Itch and looked for the presence of GFP fusions in the immunoprecipitated...
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
... X-ray analysis of tandem MIT domains of calpain and IST1 MIM elements, should clarify these issues in the future Although the physiological role of human calpain is still unknown, the presence of ... the linker region between the MIT domains of calpain is much shorter than that of Vta1 (five residues versus 21 residues), it is not certain whether the same theory applies to calpain 7, but the ... shown in Fig 9A, the effect of coexpression with FLAG–IST1 on the generation of 30 K was not so obvious regarding the ratio of precursor (arrow) and 30 K (open triangle) On the other hand, coexpression...
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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
... position 13 of the other subunit [25,26], yielding a covalently bridged dimer The 4170 oxidized and reduced forms of the Y74C mutant had very different thermal stabilities While the stability of the ... and in the presence of denaturants [27] Extending these studies, we examine here the effect of increasing the bulk of the residue at position 74 Surprisingly, the Y74W mutant exhibited loss of both ... to the WT* enzyme There are two possible reasons for the low activity of the Y74W* mutant: (a) introduction of the bulkier residue at the interface in place of a tyrosine may destabilize the...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25