... grateful to you for believing in me and letting me pursue my dreams The person I am today is because of you To my dear uncle and my aunt Khem Kandel and Laxmi Kandel: your support and friendship ... (GxxxxG) and are necessary for cofactor binding Also, residues Lys192, Glu399 and Phe401 are important for proper cofactor positioning and, thus, impact catalysis Crystal structures of mammalian ... chemotherapeutic agent and in the mobilization and conditioning regimens for blood and marrow transplantation Reviewing the chemistry and pharmacology of cyclophosphamide is crucial for understanding its...
... of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues It is quite rare for such a variety of ... resonance assignments Table Structural statistics for the family of 20 structures of conomarphin and L-Phe13-conomarphin Complete proton resonances for both conomarphin and l-Phe13-conomarphin were ... marphin were 0.54 ± 0.24 A for the backbone atoms ˚ (N, CR, and C) and 1.25 ± 0.30 A for all heavy atoms, and the values for l-Phe13-conomarphin were ˚ ˚ 0.44 ± 0.16 A and 1.22 ± 0.22 A, respectively...
... to Erlandsen and Stevens [22] The details of the interactions displayed by residues in the neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH4 and thienylalanine, ... residues are shown as ball -and- stick models (sticks of residues belonging to Ca8, to subunit A and to subunit B are drawn in yellow, cyan and green, respectively) For interaction details, see ... family and a shorter fragment of 253 bp present only in the proband, as well as in his mother who bears the same mutation (Fig 3) Direct sequencing of both cDNA bands conrmed the skipping of the...
... investigations on both 57Fe and 119Sn isotopes have been performed for a better understanding of the site occupancy of Sn4þ in the hematite lattice [12] Finally, it was found that the degree of order given ... in the presence of a few percent of (SO4)22 Figs 11 and 12 show the particle morphology and stoichiometry determined by SEM and EDX examinations of the hematite-tin oxide system for x ¼ ¨ 0:4: ... the a-Fe2O3 and x $ 0:7 for Fe3þ in SnO2 This paper is the first report on the hydrothermal synthesis andstructuralcharacterizationof (1 x)a-Fe2O3 – xSnO2 system over the full range of tin concentration,...
... and 0.1% trifluoroacetic acid in H2O The retention times of inosine and hypoxantine, guanosine and guanine were 10.5 and 4.7 min, and 11.5 and 4.3 min, respectively The amount of purine base formed ... exactly those that are responsible for the different substrate specificity of hPNP and hMTAP (Glu201, Asn243 and Val245 of hPNP instead of Ser178, Asp220 and Asp222 of hMTAP, respectively) The last ... hMTAP and hPNP reported in Fig The amino acid residues of PfPNP and Fig Multiple sequence alignment of PfPNP, PfMTAP, hMTAP, and hPNP The phosphate (w) ribose, (m) and base (d) binding sites of...
... composed of 35% a helix and a small amount of b sheet, whereas PrPSc appears to have elevated levels of b sheet [7,8] Higher resolution studies of the structure of PrPC have made use of NMR and ... spontaneous formation and subsequent accumulation of abnormal conformations of PrP Therefore, in vitro studies employing a lipidanchored prion molecule offer the potential to unravel the effect of different ... changes that lead to aggregation and ⁄ or fibrillization of PrP, depending on the lipid environment and starting conformation of the protein [33,34] The a-helical isoform of PrP, representing the cellular...
... manuscript and Dr Ch Peters and Patric Hoert (University of Freiburg, Germany) for providing the Reflex III for measurements andfor the mass spectrometry measurements We also thank to the Deutsche Forschungsgemeinschaft ... (1D, the horizontal bands) and then in nondenaturing conditions by BN ⁄ PAGE (2D) The molecular mass standards and direction of migration are indicated for both the first (1D) and second (2D) dimension ... GELGIYLVGDDSLFPWR and IRPPGFINLQILPQLVK (one missed cleavage) All of these peptides were part of the maize NdhH subunit Database analysis of a MALDI-TOF-MS measurement of a 21-kDa trypsin-digested gel band...
... glycoforms, suggesting the presence of alternate sialylated structures In this study we describe the identification andstructural analysis of a Sial-lNnT unit in H influenzae LPS MATERIALSAND ... further information on the nature of the sialylated glycoforms Comparison of the total ion electropherogram in negative ion mode (Fig 3A) and selective ion scanning for m/z 290, specific for sialic ... spectra of O-deacylated LPS from the parent strain RM118 and RM118 lgtC, Sial-Lac glycoforms were observed in addition to the higher mass sialylated glycoforms As expected Sial-Lac-containing glycoforms...
... part of projects OT/99/24 and COF/98/008, the Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT), the Fund for Scientific Research-Flanders (FWO) as part of project ... the parameters a, b and c of expression (14) and a1, b1, c1 and pH0 of expression (15) are identified Simulationsof relations (14) and (15) with the estimated parameter values for the case study ... simulating cell growth and lactic acid formation making use ofmodel Eqs (1) – (8), time-dependent courses of LaHtot, pH and [LaH] are obtained Next, through elimination of time, plots of [LaH] versus...
... consisted of 30 cycles of 94°C for min., 58°C forand 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C forand 72°C for mins C1F &C2R: PCR conditions consisted of 94°C ... PCR conditions consisted of 30 cycles of 94°C for min, 58°C forand 72°C for mins JSP001 &JSP002: PCR conditions consisted of 30 cycles of 94°C for min, 45°C forand 72°C for mins Uni F &Uni R: ... consisted of 94°C for 10 mins followed by 35 cycles of 94°C for 30 secs., 60°C forand 72°C for 1.5 mins followed and extension of mins at 72°C AC1F &AC1R : PCR conditions consisted 94°C of mins followed...
... consisted of 30 cycles of 94°C for min., 58°C forand 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C forand 72°C for mins C1F &C2R: PCR conditions consisted of 94°C ... PCR conditions consisted of 30 cycles of 94°C for min, 58°C forand 72°C for mins JSP001 &JSP002: PCR conditions consisted of 30 cycles of 94°C for min, 45°C forand 72°C for mins Uni F &Uni R: ... consisted of 94°C for 10 mins followed by 35 cycles of 94°C for 30 secs., 60°C forand 72°C for 1.5 mins followed and extension of mins at 72°C AC1F &AC1R : PCR conditions consisted 94°C of mins followed...
... Aim and Scope of Study Chronic pain associated with neuroinflammation and nerve injury is often unrelenting and difficult to treat, impacting the quality of life of an individual A number of pain ... key tool in understanding the status and functions of the CNS, and investigating the mechanisms of chronic pain The importance of biological markers for assessing the extent of chronic pain or ... impacting the quality of life of patients suffering from it 1.1.4.2 Osteoarthritis The most common form of arthritis, osteoarthritis is one of the main causes of chronic pain and long-term disability...
... PROPERTIES AND MACROSCOPIC CHARACTERIZATIONOF COMPOSITE MATERIALS QIU CHENGWEI B ENG., UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA, 2003 A DISSERTATION SUBMITTED FOR THE JOINT DEGREE OF DOCTOR OF ... intensity of the plasmonic rod of k0 a = 0.1 and r = −1 in the cases of first two terms 40 2.17 The energy intensity of the plasmonic rod of k0 a = 0.1 and r = −1 in the case of ... configuration of a chiral medium composed of the same handed wire-loop inclusions distributed uniformly and randomly 102 4.2 The frequency dependence of relative ( + , µ+ ) in the range of [5, 25]...
... Here, we present the mesoscopicmodelof biological protein materials composed of protein crystals prescribed by Go potential forcharacterizationof elastic behavior of protein materials Specifically, ... potential in the form of Eq (4) consists of potential for backbone chain stretching and the potential for native contacts Go potential is a versatile modelfor protein modeling such that Go model enables ... topology of protein structure is responsible for mechanical properties of protein crystals MODELS MESOSCOPICMODELFOR BIOLOGICAL PROTEIN MATERIALS We assume that the mechanical response of biological...
... analysis of cDNA coding for Der p 3.3.2 SDS-PAGE analysis of native and recombinant Der p 3.3.3 Presence of isoforms in native Der p 3.3.4 Comparison of IgE reactivity to recombinant Der p and native ... murine modelfor allergic dermatitis and asthma through skin sensitization, and to further exploit the modelfor mechanistic studies The first part of this study focused on the cloning andcharacterization ... severity of AD was a risk factor for subsequent development of asthma and allergic rhinitis Approximately 60% of patients experience the development of signs of AD before their first birthday, and...
... 3.1 StructuralCharacterizationof Inhibitory Domains of Nogo 54 3.1.1 Expression andstructuralcharacterizationof NogoA-(567-748) and Nogo-66 55 3.1.2 CD and NMR characterizationof Nogo-40 60 ... 3.6 StructuralCharacterizationof NgBR 134 3.6.1 Cloning and expression of NgBR and its dissected domains 136 3.6.2 Bioinformatics characterization 138 3.6.3 Structuralcharacterizationof the ... 3.3.4 CD and NMR characterizationof the Nogo-B N-terminus 99 Figure 3.3.5 CD and NMR characterizationof the Nogo-33 101 Figure 3.3.6 CD and NMR characterizationof the first group of the dissected...
... strength of the solvent environment and ⁄ or presence of small organic compounds (dioxane and MPD) seems to favor formation of dodecahedra over isolated pentamers Previous structural analysis of the ... jellyroll domain formed by two b-sheets and a distal domain formed by insertions between the strands of the jellyroll domain (Fig 3) Antiparallel b-sheets made up of strands CHEF and BIDG pack against ... those for hAd2 For hAd2pb, high concentrations of ammonium sulfate (1.6 m) and dioxane (10%) were necessary for dodecahedra formation [20], whereas hAd2 ⁄ 12pb crystallized in the dodecahedral form...
... left-right dual of a leftcorner transform It transforms all right recursive sequences in each tree into left recursive sequences of symbols of the form A1 /A2 , denoting an incomplete instance of category ... rules, and therefore potentially makes better use of limited data Evaluation The evaluation of this system was performed on the Switchboard corpus, using the mrg annotations in directories andfor ... While an integrated system for processing and parsing speech may use both acoustic and syntactic information to find repairs, and thus may have access to some of this information about where interruptions...
... improve the modeling of fragments Table 2: Length of Reparandum Offset Word Fragments (N=288) bution of initial phonemes for all words in the corpus of 6,414 ATIS sentences, andfor all fragments, ... here are for prediction on only TI repair utterances 6We performed the same analysis for the last and first syllables in the reparandum and repair, respectively, andfor normalized f0 and energy; ... marking the reparandum offset From the point of view of repair detection and correction, acoustic-prosodic cues to the onset of the reparandum would clearly be useful in the choice of appropriate...
... loops of EF-hand proteins, have chemical shifts 10 p.p.m for filled loops and chemical shifts of < p.p.m for empty loops [50] The chemical shifts of CR I–II, Calb I– II and Fig ‘Glycine 6’ of the ... single bands of monomeric weight and S100B provides a positive control with a yield of cross-linked dimer of < 30% The CR I – II and Calb I – II sequences have high sequence identity, Fig 1, and ... fragments correspond to CR(1–60) and CR(61–100) for the upper and lower bands, respectively Fig 1H,15N HSQC of 15N-labeled CR I –II with assignments Of note are residues G34 and G81, the ‘position 6’...