mechanochemical coupling in molecular motors insights from molecular simulations of the myosin motor domain
Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf
... perturbation of the DNA-binding interface showed that, in the case of HPV-16, most of the binding energy comes from a ‘direct readout’ recognition mode [22] In fact, recognition of DNA by proteins, in ... mutation into arginine, indicating the importance of the positive charges for the occurrence of nonspecific contacts with DNA [22] The BPV-1 protein shows low flexibility of the loop connecting the ... a regular helix, in particular at the level of the Phe303 residue [13] Interestingly, these deviations are maintained also in the DNA-bound form [13] In the HPV-16 protein, the b2–b3 loop is...
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Báo cáo y học: "Advances in Molecular Diagnosis of HBV Infection and Drug Resistance"
... [23], the first two include the substitution of methionine (M) by the amino acids isoleucine (I) or valine (V) in the YMDD motif (C domain) at position rtM204V/I In the majority of cases, these ... mutations in the YMDD motif occur together with an additional compensatory mutation in the B subdomain [20], namely the substitution of a leucine by methionine some 20 amino acids upstream from the ... the YMDD domain at position rtL180M Finally, the newly discovered mutant to adefovir (rtN236T) is located downstream from the YMDD motif in the D domain of the viral polymerase Assuming correct...
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
... Mapping the transcription start site of the xMGP gene Fig Sites of intron insertions within the amino-acid sequence of Xenopus, human and mouse MGPs Conserved sites of intron insertions in mammalian ... indicates that all other introns (2, and 4) are located at conserved sites within the MGP coding sequence (Fig 2) Analysis of the phase of each of the xMGP introns located within the coding region revealed ... subcloned into pBSSK (Stratagene) The structure of the gene including the 5Â and 3Â anking regions was determined by double-stranded DNA sequencing, exons were identied according to the sequence of the...
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Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc
... of several amino acid side chains Amino acid residues interacting with the guanine moiety of GDP in EhPGK The amino acid residues known to interact with the adenine moiety in the ADP ⁄ ATP-binding ... carbon of the guanine ring may interact with the side chain of Glu309, whereas the carbonyl group at position of the guanine ring may interact with the hydroxyl group of the Tyr239 side chain (Figs ... structures of the wild-type, single- and double-mutant EhPGKs in a closed conformation will certainly help to clarify the molecular arrangement in the guanine ring binding site during the binding of one...
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Báo cáo khoa học: Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida pptx
... protein serine ⁄ threonine protein kinase; MEK, MAPK serine ⁄ threonine protein kinase; MLCK, myosin light chain kinase (serine ⁄ threonine protein kinase); MLCPase, myosin light chain phosphatase; ... chain phosphatase PP1 and thereby leads to increased levels of myosin light chain phosphorylation [101] The resulting myosin light chain phosphorylation regulates actin reorganization, increasing ... receptor binding and translocation activity remains unclear, these domains are located in the N-terminus of each of these toxins and share limited sequence similarities with each other [131–137]; they...
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Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc
... obtained for four mice, indicating that fewer than 50 bacteria of the PMM56 mutant strain were present in the spleen of these mice For the determination of the number of PMM56 bacteria present in ... determined by calculating the ratio of mutant to wild-type bacteria after correcting for the ratio of these strains in the inoculum It appeared that, 21 days after infection, the ratio of PMM56 ... corresponding to that of the methine proton of the carbon bearing the methoxyl group [14,15] Furthermore, the proton resonances of the methyl group b (Fig 5) was observed at 1.05 p.p.m., instead of...
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Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt
... assist in the binding and positioning of the substrate and play a role in the recognition of the reducing end of the cellulose chain Arginine side-chains are present in all equivalent locations in ... of a Cterminal carbohydrate-binding module linked via a proline/ serine/threonine-rich linker to the catalytic domain [45] There are two predicted N-glycosylation sites in the catalytic domain ... for the backbone of CBH IB The final model, 1Q9H, included 430 of the 437 amino acid residues of CBH IB The final two amino acids and the loop region (from amino acids 193–197) were not visible in...
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Báo cáo khoa học: Molecular cloning of the ecdysone receptor and the retinoid X receptor from the scorpion Liocheles australasiae pot
... structure of the EcR ligand-binding domain ⁄ USP ligand-binding domain of Heliothis virescens with PonA was solved In the analysis of the EcR ⁄ ligand-binding domain ⁄ PonA complex, amino acid ... (ligand- or hormone-binding domain) [8,9] The X-ray crystal structures of the ligand-binding domains of EcR, USPs and RXRs have been resolved in a few insects [10–13], and the binding of ponasterone ... decemlineata [15] In these species, the specific binding of PonA to EcR was significantly enhanced in the presence of USP The heterodimerizing effect of USP on ligand–receptor binding is common to the...
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báo cáo hóa học:" Molecular analysis of the apoptotic effects of BPA in acute myeloid leukemia cells" potx
... reproduced in many other cells These evidences are exciting from several point of view: if from one side we might consider the induction of apoptosis as an interesting anti-cancer action, on the other ... by the double treatment, showing an increase of the G1 peack at low dose BPA (30 μM) and an increase of the G2-M fraction of cells at the highest concentration of BPA (100 μM) Differently, in the ... peack in all the cell lines tested, HL60 being the most resistant one In NB4 cells, a model from promyelocytic leukemia containing the fusion protein PMLRARα and sensitive to retinoids, the highest...
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báo cáo hóa học:" Mass spectrometry-based serum proteome pattern analysis in molecular diagnostics of early stage breast cancer" pdf
... could reflect the low resolution of the analyzer operating in the linear mode or might result in overlapping of ions originating from protein/ peptides of very similar molecular masses In addition, ... Data Processing and Statistical Analysis The preprocessing of data that included averaging of technical repeats, interpolation of missing or non-aligned points, binning of neighboring points to reduce ... is the study of the proteome, the complete protein components of the cell, tissue or organism, which in contrast to the genome is dynamic and fluctuates depending on a combination of numerous internal...
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Báo cáo khoa học: "Molecular dissection of the quantitative inheritance of wood property traits in loblolly pine" pps
... are the same as the paternal grandparents of the qtl pedigree Therefore the prediction mother and the qtl father are full-sibs Also, the paternal grandmother of the prediction pedigree is the ... that the microfibrils of the S2 layer of the cell wall make with the longitudinal axis of the cell [20] Lower fibril angles (closer alignment with the axis of the cell) have a positive influence ... defined as the non-random association of alleles at linked loci and results from the two sites only rarely recombining from each other; it is an indirect estimate of how closely two loci are linked...
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Molecular characterization of the PhoPQ-PmrDPmrAB mediated pathway regulating polymyxin B resistance in Klebsiella pneumoniae CG43 ppt
... Analysis of EMSA indicates a direct binding of the recombinant PhoP to pmrD The binding of PhoP or PmrA to PpmrH has been determined recently [31] In order to determine whether PhoP binds directly ... after the addition of deferoxamine The results clearly demonstrate the involvement of PmrA, PhoP and PmrD in the regulation of the expression of pmr genes, particularly in the presence of high ... shown in Figure 5A, binding of the recombinant HisPhoP protein to PpmrD was evident by the formation of a protein/DNA complex with a slower mobility The binding specificity was also examined by the...
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báo cáo khoa học: "Updates in Gastrointestinal Oncology – insights from the annual meeting of the American Society of Clinical Oncology" potx
... elements, including early administration of mitomycin-C, inclusion of cisplatin and prolonged doxifluridine administration besides the intraperitoneal therapy Thus, it remains to be proven if the survival ... the OFF arm The median OS in the OFF arm was 28 weeks, and that of the FF arm was 13 weeks, thereby fulfilling the study hypothesis There was also a significant prolongation of PFS in the treatment ... examined intermittent administration of oxaliplatin in minimizing neurotoxicity from oxaliplatin The Combined Oxaliplatin Neurotoxicity Prevention Trial (CONcePT) randomized patients receiving...
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Báo cáo khoa học: "Molecular characterization of the Great Lakes viral hemorrhagic septicemia virus (VHSV) isolate from USA" pot
... N-terminal half of the P protein of VHSV is highly varia- ble, whereas C-terminal half is conserved Phylogenetic tree of the P protein shows clustering of MI03GL, JF00Ehil and Makah strains The ... residue at the 5'-terminal Figure shows the phylogenetic trees formed by comparing the deduced amino acid sequences of MI03GL strain N, P, M, NV and L proteins with other VHSV strains, in which ... Fi13 strain [9] and coding regions of several other strains of VHSV have been determined later [10] In this study, we characterized the entire genome of the Great Lakes VHSV isolate MI03GL from muskellunge,...
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Báo cáo khoa học: "Molecular characterisation of the early response in pigs to experimental infection with Actinobacillus pleuropneumoniae using cDNA microarrays" doc
... expression profiles of the samples into two groups – one group containing profiles from inoculated animals/ inflamed tissues and one group containing profiles from the non-inoculated animals/non-inflamed ... Veterinaria Scandinavica 2007, 49:11 mRNA coding for the anti-inflammatory cytokine IL-10, found in inflamed areas of the lung, is probably due to the function of IL-10 in counteracting the host ... rows representing profiles of genes and columns representing profiles of samples The gene dendrogram is shown to the left of the matrix and the dendrogram of the samples is shown above the matrix...
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Báo cáo y học: " The role of the humoral immune response in the molecular evolution of the envelope C2, V3 and C3 regions in chronically HIV-2 infected patients" pdf
... below), in the envelope complex of HIV-2 differs substantially from that of HIV-1 Glycans on HIV-1 envelope protein play an important role in the folding of the glycoproteins, in infection and in ... is the main driver of the rapid intra-host evolution of the C2 and C3 regions in HIV-2 [15,25,58,60,64-66] Indeed, we found that most of the amino acids under selection are located in C2, including ... common in HIV-2 than in HIV-1 infection Still, little is known about the role of humoral immunity in the evolution of the HIV-2 env gene In the present study we analyze, for the first time, the molecular...
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Báo cáo sinh học: " Molecular evolution of the leptin exon 3 in some species of the family Canidae" pptx
... determine intraspecies polymorphisms in the coding sequence (exons and 3) of the canine leptin gene and the detection of a mutation which occurred in the course of evolution in selected species of ... arginine Likewise, in the dog, asparagine was present at the 87th position but in the other species studied it was replaced by threonine The third substitution occurred at the 121st position in ... polymorphisms in the coding sequence of the gene [12] Diversity of the leptin gene was studied in 578 A Chmurzynska et al Table II Some polymorphisms identified in exon and exon of the human, porcine and...
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Molecular analysis of the roles of yeast microtubule associated genes in agrobacterium mediated transformation
... and Schroer, 2000) There are two types of kinesin, kinesin-1 and kinesin-2 Kinesin-1 is found to be involved in the transport of various organelles including Golgi complex (Lippincottschwartz et ... VirE2 in vitro and the binding site in VirE2 overlapped with the VirE2 self-binding domain, indicating that VirE1 may protect VirE2 from self-aggregation (Deng et al., 1999) The binding site in ... was blocked by inhibition of the minus-end directed dynein (Salman et al., 2005) This study suggests the involvement of MTs and the dynein motor in the trafficking of T-complex Nevertheless, more...
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Molecular simulations of biofuel and water purification in metal organic frameworks
... used the same force field to examine the interfacial water in MOF-5, and 22 Chapter Introduction reported the instability of MOF-5 structure in presence of water based on the vibrational modes of ... α-cage The two S8MRs form a double eight-membered ring (D8MR) 60 Figure 3.5 Adsorption isotherms of water, methanol, and ethanol in Na-rhoZMOF The inset shows the isotherms in the linear scale of ... representation of (a) Na-rho-ZMOF and (b) ZIF71 Two types of binding sites exist for Na+ ions in Na-rhoZMOF, in which site I (pink) is at the single eight-membered ring (S8MR) and site II (yellow) is in the...
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Molecular simulations of transport and separation in protein crystals
... crystalline proteins are directly packed in chromatographic column.5 Furthermore, the compact arrangement of protein molecules in crystalline phase inhibits protein unfolding and thus maintains their ... identical with the same nature of the protein molecule Both of them are larger than those in the thermolysin 1L3F crystal because of the higher porosity in the latter We also estimated the hydration ... ratio of the number of water molecules in the protein crystal to the total number of bulk water molecules that can fill in the simulation box without protein The bulk water density of the SPC...
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Tìm thêm: xác định các mục tiêu của chương trình xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25