mechanisms of epigenetic gene activation in disease dynamics of dna methylation and demethylation
báo cáo khoa học: " Dynamics of DNA methylation and Histone H4 acetylation during floral bud differentiation in azalea" doc
Ngày tải lên :
12/08/2014, 03:21
... 1: tubulin and AcH4 of bud in phase A; Lane 2: tubulin and AcH4 of bud in phase B; Lane 3: tubulin and AcH4 of bud in phase C; Lane 4: tubulin and AcH4 of bud in phase D Marker bands are indicated ... effective handling of the timing of flowering, and the improvement of flowering in quantity and quality Cellular reprogramming, marked by changes on the levels of global DNA methylation and H4 acetylation ... acetylation of specific lysine residues within the amino-terminal tails of H3 and H4 and DNA demethylation are positively correlated with open chromatin configuration and gene activity in meristematic...
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Báo cáo khoa học: Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells potx
Ngày tải lên :
30/03/2014, 02:20
... specific inhibitor of the JAK-STAT pathway) for 16 h [24], indicating that the activation of STAT5B and c-Jun may be involved in Ang II-induced AGT mRNA expression (Fig 1C) DNA- binding activity of ... relationship between the activation of STAT5B and c-Jun, and STAT5B and AP-1 interaction in AGT gene activation the expression of the AGT gene in VSMCs stimulated with Ang II, the effect of Ang II on the ... of STAT5B binding and of c-Jun binding to DNA was detected, respectively, by electrophoretic mobility shift assays (EMSAs) using radiolabeled oligonucleotides containing either a STAT5B-binding...
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Báo cáo khoa học: Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc
Ngày tải lên :
08/03/2014, 08:20
... level of BACE protein, and in consequence, to reduce the amount of Ab peptide released in neuronal cells We asked the question whether we can inhibit expression of the gene of BACE protein using ... expression system is of interest for endogenous generation of siRNA and activation of the RNAi effect in human cells [45] Studies on an inhibition of BACE gene expression by endogenously generated siRNA ... scale Constructs containing inactive versions of Rz-1 and Rz-2 were obtained in the same way by using templates as described above but in which the guanosine nucleotides, marked in bold, had been...
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Báo cáo sinh học: "Comparing the retention mechanisms of tandem duplicates and retrogenes in human and mouse genomes" pdf
Ngày tải lên :
14/08/2014, 13:21
... tandem duplicates and 410 retrogenes in the human genome, and 1,802 tandem duplicates and 680 retrogenes in the mouse genome The higher number of tandem duplicates than retrogenes in both genomes ... same in yeasts and mammals [22-25] To investigate the difference in gene duplicability between tandem duplicates and retrogenes in more detail, we counted the number of each type of duplicates in ... with the intervening spacers containing less than 10 genes, since they were confused with tandem duplicates Finally, we preserved 410 and 680 pairs resulting from We identified 1,210 tandem duplicates...
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THE ROLE OF DNA METHYLATION IN REGULATING LHX3 GENE EXPRESSION
Ngày tải lên :
24/08/2014, 09:47
... contain a DNA- binding homeodomain, two LIM domains, which participate in protein-protein interactions, and a carboxyl terminus (C-terminus) trans -activation domain The DNA- binding homeodomain also ... other binding proteins, such as methyl CpG-binding proteins Methyl-CpG binding proteins (MBPs) bind methylated DNA and recruit regulatory proteins to modulate chromatin structure and gene activity ... of DNA silencing involve alterations in protein -DNA interactions, and thus implicated histones and other transcription factors in the mechanism Studies examining the interactions of methyl-binding...
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THE MECHANISMS OF DNA REPLICATION pot
Ngày tải lên :
30/03/2014, 00:20
... Moriyama and Naoki Sato Section Chromatin and Epigenetic Influences on DNA Replication 313 Chapter 12 Roles of Methylation and Sequestration in the Mechanisms of DNA Replication in some Members of ... recognize and bind to the protein -DNA structure formed by the initial unwinding of the ORI DNA Owing to its ssDNA binding capability RPA associates with the RC once unwinding of the ORI DNA is underway; ... phosphorylation of Sld3, and Sld2 recruit Sld2, Dpb11, Pole and GINS to the Mcm2-7 complex GINS binding increases the helicase activity of the Mcm2-7 hexamer allowing unwinding of duplex DNA The association...
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Báo cáo khoa học: Modeled ligand-protein complexes elucidate the origin of substrate specificity and provide insight into catalytic mechanisms of phenylalanine hydroxylase and tyrosine hydroxylase pptx
Ngày tải lên :
31/03/2014, 07:20
... BH4 in PAH is shown in red, for BH4 in TH according to the results of the automated docking in blue, and for BH4 in TH according to the manual docking in the X-ray crystallographic mode in green ... other hand, according to the experimentally established sequence of substrate binding in TH [6], the binding of dioxygen prior to the amino acid will promote the placement of tyrosine in the ... Whether binding of tyrosine in this position triggers a large change in the protein structure as shown after binding of 3-(2-thienyl)-L-alanine [22] needs to be investigated Feedback inhibition...
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Báo cáo khoa học: "Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-g" doc
Ngày tải lên :
07/08/2014, 23:22
... and Cyclosporin A resulted in a lack of protection against IBDV following immunization with an inactivated IBDV vaccine [34] Further, priming with in ovo DNA vaccine and boosting with recombinant ... levels of cellmediated immunity against pathogens [30], and increased the antibody response to the vaccine [18] DNA vaccination against IBDV involves priming with DNA vaccine and boosting with ... fixed in 10% buffered formalin After routine processing, the tissues were embedded in paraffin, cut into approximately 3-μm sections, and prepared for hematoxylin and eosin (H&E) staining for...
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Báo cáo sinh học: " The effects of DNA formulation and administration route on cancer therapeutic efficacy with xenogenic EGFR DNA vaccine in a lung cancer animal model" pps
Ngày tải lên :
14/08/2014, 19:22
... Efficacy of Gene Gun Administration of Non-coating EGFR DNA vaccine The efficacy of gene gun administration of non-coating EGFR DNA vaccine was the best among three types of EFEGFR DNA vaccines, and ... Sec-N'-EGFR DNA vaccine four times at weekly intervals via three different methods: intramuscular injection (i.m), gene gun administration of gold-coated DNA, and gene gun administration of noncoating DNA ... role in mediating therapeutic efficacy of gene gun administration of noncoating EGFR DNA vaccine Discussion Figure Tumor infiltration of CD4+ and CD8+ T cells Tumor infiltration of CD4+ and CD8+...
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Aggregate and spatial distributions of DNA palindromes and their applications to replication origins prediction in some viral genomes
Ngày tải lên :
15/09/2015, 21:52
... translation of genes into proteins, transferring amino acids to the ribosome to form proteins, and also translating the transcript into proteins The definition of a RNA palindrome is similar to that of ... (3): Computing the Window score: The score of a window in the genome is simply the total of the scores of all the palindromes occurring in this window A palindrome is considered in the window if ... strand of DNA is a chain of chemical “building blocks”, called nucleotides, of which there are four types: adenine (abbreviated A), cytosine (C), guanine (G) and thymine (T) Between the two strands,...
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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
Ngày tải lên :
09/08/2014, 23:20
... the binding sites, to assess whether the factor is being affected by specific changes in methylation of the binding site, instead of more general methylation changes in the surrounding region In ... splicing machinery is in any way regulated by the methylation of these sites, it is intriguing that this is yet one more piece of evidence indicating that splicing at chromatin are coupled with DNA ... differential methylation in binding sites and their associated expression, suggesting that their binding affinities are affected by the DNA methylation status of the target sequence We found that most of...
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Báo cáo sinh học: "WordCluster: detecting clusters of DNA words and genomic elements" docx
Ngày tải lên :
12/08/2014, 17:20
... analysis of word clustering and help with the interpretation of statistical results, GB and AMA retrieve and organize the genome and methylation databases, and JLO developed the algorithm and wrote ... that this is generally the case for short distances, thus indicating the clustering (overrepresentation of short distances) of CpG dinucleotides The intersection defines the “reversal point”, i.e ... http://www.almob.org/content/6/1/2 clusters of CpGs (CpG islands) using different distance models, 2) detection of clusters of the word CWG (where W = A, T) and 3) detection of clusters of olfactory receptor genes in the human...
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Development of gold nanoparticle DNA nanostructure assembly for detection of DNA, RNA and protein biomarkers
Ngày tải lên :
09/09/2015, 11:16
... hairpin DNA- functionalized AuNPs were reported for the sensing of mRNA in melanoma cells, with the binding of mRNA targets resulting in the restoration of beacon fluorescence and allowing intracellular ... Typically, distinct bands were observed only for conjugates carrying the longer and more massive 100-base single strand DNA (ssDNA) With shorter ssDNA, such as 18b ssDNA, the distinct bands were not ... bearing one strand of ssDNA; (D) dsDNA-linked AB dimeric AuNPs The particle size distribution of different AuNPs systems before and after addition of 10 nM of ERβ are indicated by the ‘empty’ and...
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Development and application of novel capillary electrophoresis techniques for analysis of DNA fragments and organic pollutants
Ngày tải lên :
14/09/2015, 11:40
... applications in DNA analysis and monitoring of pollutants are of significant importance 1.3.1 CE Application in DNA Analysis DNA separation by CE has been the subject of intensive research during the ... electrolyte contains ion with the low mobility When sample ions with mobilities between those of the leading and terminating ions are introduced between the leading and terminating ions, the ions ... Figure 3.13 Electrophoresis of ΦX174 DNA in Buffers containing Different Concentration of GNPs-a 100 Figure 3.14 Electrophoresis of ΦX174 DNA in Buffers containing Citrate or GNPs-a ...
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Tài liệu Báo cáo khoa học: Models and mechanisms of O-O bond activation by cytochrome P450 A critical assessment of the potential role of multiple active intermediates in oxidative catalysis doc
Ngày tải lên :
19/02/2014, 16:20
... benzene ring of aniline to yield p-aminophenol [151] Formation of the oxygenating species has been recognized to be facilitated by the binding to HbO2 of aniline and some of its derivatives, causing ... (1983) Mechanisms of extrahepatic bioactivation of aromatic amines: the role of hemoglobin in the N-oxidation of 4-chloraniline In Extrahepatic Drug Metabolism and Chemical Carcinogenesis (Rydstrom, ... Kitagawa, T & Makino, R (1993) Roles of proximal ligand in heme proteins: replacement of proximal histidine of human myoglobin with cysteine and tyrosine by sitedirected mutagenesis as models...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
Ngày tải lên :
19/02/2014, 16:20
... p38-dependent activation of the CTGF/CCN2 gene although RhoA GTPase-independent required an intact cytoskeleton, and, at least in part, the upstream activation of PtdIns 3-kinase Inhibition of PtdIns 3-kinase ... for 30 and then fixed, permeabilized and stained for F-actin with rhodamine-conjugated phalloidin The effects of toxin B and latrunculin B on the actin filaments was examined by preincubating the ... F-actin bundles appear shorter and contained significantly less branching, consistent with the role of swinholide A as a promoter of G-actin dimerization Interestingly, both jasplakinolide and swinholide...
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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt
Ngày tải lên :
16/03/2014, 18:20
... modulators of dopamine neurotransmission, and abnormalities in cannabinoid signalling or modulation of dopamine signalling or both have been implicated in a number of neurodegenerative diseases including ... expression of CB1 in isolated cortical neurons was affected in both strains of R6 transgenic HD mice It appeared that the amino terminus of mutant huntingtin containing an expanded polyglutamine tract ... levels of mutant huntingtin protein are lower in the R6/1 mice compared to R6/2 [10] and that neuronal intranuclear inclusions (NIIs) containing the human transgene-encoded amino terminus of human...
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Báo cáo khoa học: Adeno-associated virus gene transfer in Morquio A disease – effect of promoters and sulfatase-modifying factor 1 pot
Ngày tải lên :
29/03/2014, 21:20
... for gene therapy, because of its capacity to induce transgene expression in a wide range of tissues, and the long-term therapeutic levels of expressed proteins observed in different diseases and ... However, in vitro [60] and in vivo [61] studies have shown that AAV vectors induce a change in gene expression profile Genes involving cellular proliferation and differentiation, DNA replication, DNA ... which increase mRNA instability and production of the target protein [43,44] The results presented in this work agree with previous reports showing that the inclusion of a synthetic intron and...
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báo cáo hóa học: " Type D personality in the general population: a systematic review of health status, mechanisms of disease, and work-related problems" docx
Ngày tải lên :
18/06/2014, 19:20
... and experimental evidence regarding the role of Type D personality in potential mechanisms of disease as well as work-related Type D personality and mechanisms of disease Type D personality and ... 2), Canada, Ireland, Poland, and Ukraine The results of the studies included in this review are first described below according to the impact of Type D personality on mental and physical health ... Six studies examined behavioral and biological mechanisms of disease as a function of Type D personality in apparently health individuals (Table - section a) Regarding behavioral mechanisms, two...
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