measuring metals and metalloids in water sediment and biological tissues
Techniques in Aquatic Toxicology Volume II - Section 4 ppt
... (Sand Hill Engineering, Geneva, FL) software interface on a Macintosh operating system Dusk–dawn lighting systems are very useful in reducing stress in aquatic holding and testing facilities, and ... significant progress in the ease, utility, and affordability of obtaining, interpreting, and applying behavioral endpoints in a variety of applications from water quality monitoring to use in toxicity ... NH2 scissoring vibrations of cytosine In- plane ring vibrations of cytosine d, 1576 cm−1 Thymine ring vibrations; adenine NH2 bending and C N stretching vibrations e, 1525 cm−1 Cytosine residue;...
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báo cáo khoa học: " High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes" doc
... contain three domains: an amino-terminal domain, a carboxyl-terminal domain and a central catalytic domain [27,34] The N-terminal region is Page of 14 important for specifying the chain length and ... substrate binding sites and in the protein secondary structure In Figure we show as an example the case of SBEIIa-D (V398I) : while in wild type protein residue 398 was involved in the b3 strand of ... crossing single null genotypes and selecting the F2 progeny as described in Material and Methods Pyramiding of two null homoeoalleles results correlated with an increase in amylose content included...
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Báo cáo sinh học: "A microsatellite-based analysis for the detection of selection on BTA1 and BTA20 in northern Eurasian cattle (Bos taurus) populations" doc
... genomic interval of significant LD is comparable with the findings of hitchhiking around two anti-malarial resistance genes in humans [58] and microsatellite hitchhiking mapping in the three-spined ... (AGLA17 and SOD1) probably linked to the candidate gene for the polled trait in the populations investigated The polled trait is an autosomal dominant trait in cattle and to date the genes controlling ... comparisons involved AGLA17 or SOD1 in the comparisons between Finnish native populations (Northern Finncattle, Eastern Finncattle and Western Finncattle) vs the native populations from Russia and Ukraine...
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Báo cáo khoa học: "Comparison of Diagnostic Methods for the Detection of Parasites in Fish" pdf
... recovered and placed in separate vials with PBS The content of selected separate organs (oesophagus, stomach, pyloric caeca and intestine) was scraped from the lumen and epithelial lining and inspected ... (Trematoda, Digenea) Int J Parasitol 28: 1403-1411 Lysne, D.A., W Hemmingsen, and A Skorping (1995) Pepsin digestion reveals both previous and present infections of metacercariae in the skin of fish Fish ... weighed, ground in a mortar with pestle and transferred into a beaker (1:5 to 1:10 w/v) with pepsin solution (2% pepsin, pH 2) at acid conditions They were mixed well and placed in a 37oC incubator...
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Báo cáo khoa học: "Development of immunoassays for the detection of kanamycin in veterinary fields" pps
... noitceted eht rof dohtem gnineercs elpmis dna dipar a sa ydobitna detagujnoc -dlog ladiolloc gnisu depoleved saw yassa cihpargotamorhc -onummi na ;klim dna amsalp lamina ni nicymanak fo noitceted ... elpmas detulid lµ 05 fo erutxim a gniniatnoc llew eht otni deppid saw pirts tset eht fo egde rewol eht ,gniyrd retfA )mm 5.4 × 52 ,531 FH( pirts enarbmem esolullecortin eht fo dne eno ot deilppa saw ... syad evitucesnoc rof yad/gk/gm 02 ta stibbar ot ylralucsumartni deretsinimda saw nicymanaK noitartnecnoc doolb fo gnirotinoM noitaived dradnats eht semit eerht sulp slevel dnuorgkcab naem eht...
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Báo cáo y học: "Computerized two-lead resting ECG analysis for the detection of coronary artery stenosis" pps
... to 2000 in more than 30 institutions in Europe, Asia, and North America on individuals of varying ages and degrees of disease state including normal populations [17,18] All ECG analyses in this ... site were blinded to all 3DMP findings The 3DMP technicians and all Premier Heart staff were blinded to all clinical data including pre-test probabilities for CAD or angiography findings from the ... harmonics in the frequency domain from each lead independently but also on other linear or non-linear correlations between the two leads in both frequency and time domains, in combination 3DMP...
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Báo cáo y học: " Computerized two-lead resting ECG analysis for the detection of coronary artery stenosis after coronary revascularization" doc
... to 2000 in more than 30 institutions in Europe, Asia, and North America on individuals of varying ages and degrees of disease state including normal populations [20,21] All ECG analyses in this ... blinded to all 3DMP findings The 3DMP technicians and all Premier Heart staff were blinded to all clinical data including pre-test probabilities for CAD or angiography findings from the study ... Systematic review and modelling of the investigation of acute and chronic chest pain presenting in primary care Health Technol Assess 2004; 8: 1-158 Anthony D Diagnosis and screening of coronary...
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Báo cáo khoa học: " Comparisons of Sampling Procedures and Time of Sampling for the Detection of Salmonella in Danish Infected Chicken Flocks Raised in Floor Systems" pptx
... Salmonella positive flocks in routine samples and in 5sock-samples for the remaining 26 broiler flocks In 16 flocks, where Salmonella was detected in the routine samples (ranging from to Salmonella ... measures in parent rearing and breeder chicken flocks However, in December 1996 further legislation instituting the Danish Salmonella Control Programme was introduced in order to particularly intensify ... raised in a floor system in the same house during the same period, and infected with S enterica prior to sampling Houses with broilers, rearing stock on floor, and table egg layers in either free range,...
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Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)
... http://www.medsci.org Int J Med Sci 2009, analyses included in the database were performed using the same “made in USA” equipment as in the included trials and were analyzed using the same software and hardware ... study site were blinded to all MCG results and findings The MCG technicians and all Premier Heart staff were blinded to all clinical data including pre-test probabilities for CAD and the coronary ... safe, and immediately accessible diagnosis on the Internet for patients in a wide variety of care settings including EMS, Urgent Care Facilities, Emergency Rooms, and in- or out-patient clinics/hospitals...
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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens
... S9mix using TA98 and YG1041 2-Nitronaphthalene, 2,4-dinitrotoluen and 4-nitroquinoline N -oxide (4-NQO) are tested without S9mix, 2-aminoanthracene and benzo[a]pyrene are tested with S9mix using ... P.P and Kadluber, F.F (1987) Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and ... Relationship between mutagenicity in YG3003 and 8-OH-Gua level in rat hepatocytes induced by chemicals Fig Chemicals tested are Aflatoxin B1, 2-aminoanthracene, 2-aminoanthraquinone, 3-nitrofluoranthene,...
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ag doped wo3-based powder sensor for the detection of no gas in air
... and their heterogeneity may explain the poor re¯ection hump obtained in XRD) Typically, as seen in the ®gure, a region containing ca Ê 6.93 A inter-planar separation can be visualized It is interesting ... method using g Na2WO4 (Aldrich, 99.8%) pre-dissolved in 100 ml DM water, followed by spraying into a M HCl solution with stirring at 80 8C shown to be exceptionally effective in enhancing the ... sensor materials were measured in a ¯owing stream of pure air or in 40 ppm NO in air (60:40 mixture from an air cylinder with another cylinder (BOC special gases) containing ca 100 ppm NO balanced...
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Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx
... within macaque RV2 rhadinovirus species but not within macaque RV1 rhadinoviruses In this paper, we report the development of a sensitive and specific TaqMan QPCR assay and its use in detecting and ... RRV and the closely related MneRV2 within the RV2 lineage distinct from RFHV and KSHV [5] Although RV2 rhadinoviruses have been identified in all Old World non-human primates tested, including ... [3,4] We and others have demonstrated the existence of two distinct lineages of KSHV-like rhadinoviruses in Old World non-human primates [5,6] The rhadinovirus-1 (RV1) lineage includes KSHV and closely...
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báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot
... within macaque RV2 rhadinovirus species but not within macaque RV1 rhadinoviruses In this paper, we report the development of a sensitive and specific TaqMan QPCR assay and its use in detecting and ... RRV and the closely related MneRV2 within the RV2 lineage distinct from RFHV and KSHV [5] Although RV2 rhadinoviruses have been identified in all Old World non-human primates tested, including ... [3,4] We and others have demonstrated the existence of two distinct lineages of KSHV-like rhadinoviruses in Old World non-human primates [5,6] The rhadinovirus-1 (RV1) lineage includes KSHV and closely...
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Báo cáo hóa học: " Unscented Kalman filter with parameter identifiability analysis for the estimation of multiple parameters in kinetic models" pptx
... describes an incomplete and noisy observation model, and both w and v are uncorrelated white noises of the system and measurement model, respectively During the UT, sigma points, a minimal set ... recursion the availability of new information during the parameter estimation in UKF correlates to a general decrease in the uncertainty within the system [21], making the standard deviation a feasible ... applied in this study, which by definition only ensures that the system is identifiable within a finite (but not unique) set of points in the parameter space Individual parameters within this...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
... hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled probe, 100 µg/ml proteinase ... cells infected with 104 TCID50 By using digoxigenin-labeled probe, ISH of MDCK cells infected with 107 TCID50 of virus resulted in strong positive signal in the nucleus and cytoplasm as distinct ... differences in the detection limit of ISH However, digestion with 200 µg/ml proteinase K caused some tissue degradation and increased background staining Digestion with 100 µg/ml proteinase K induce...
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Báo cáo khoa học: "Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine" pptx
... yb selpmas mures evitisop 501 fo tuo enin-yteniN )4 dna giF( )408.0 = r( tset NS dna )768.0 = r( IH lanoitnevnoc eht dna ASILE-I neewteb noitalerroc tnacifingis a dewohs sesylana evitarapmoC stset ... nosaM ,E ihsinoK 82-12 ,7 ,3891 sdohteM loriV J suriv sitilahpecne esenapaJ ot seidobitna fo noitceted rof doolb elohw fo yassa tnebrosonummi deknil-emyzne dipaR M akoamaY ,E ihsinoK 352-742 ... eht ot etacilpud ni dedda erew selpmas mures detulid eht fo sretilorcim ytfiF )02 neewT %50.0 gniniatnoc 2.7 Hp SBP( T-SBP htiw semit eerht gnihsaw yb dewollof saw hcihw ,h rof Co73 ta )KU ,segareveB...
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Báo cáo khoa học: " A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples" potx
... USA) were cut into 4.5 mm × 7.5 cm strips Streptavidin was diluted in 0.4 M NaHCO3/Na2CO3 buffer (pH 9.0) containing 5% methanol in a final concentration of 20 pmol/μl Streptavidin was spotted ... additional 1.5 h in a vacuum oven (-15 o psi) at 55 C Subsequently the membranes were incubated in a blocking solution of 0.5% polyvinylpyrrolidone, 0.015% casein in Tris-buffered saline (TBS, 20 ... wash buffer (PBS containing 0.05% [v/v] Tween-20 and 0.01% bovine serum albumin), and once with 200 μl of PBS To each well, 100 μl of biotinylated capture probe (0.1 μM in 50 mM potassium phosphate...
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Báo cáo y học: "Reliability of computerized image analysis for the evaluation of serial synovial biopsies in randomized controlled trials in rheumatoid arthritis" pps
... the reproducibility of measuring cytokine and cell adhesion molecule staining by DIA was reported to be within 10% [8], no formal studies investigating intrarater and inter-rater variability ... advantage of DIA is standardization of image acquisition and processing, minimizing variance, and the ability to quantify the actual stained area together with staining intensity in a time efficient ... section are selected During analysis, staining thresholds are set for primary staining (i.e CD3+ T lymphocytes), nuclear staining and background staining The output is generated in a spreadsheet as...
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Báo cáo khoa học: "Study protocol of the German "Registry for the Detection of Late Sequelae after Radiotherapy in Childhood and " ppsx
... study and are involved in continuing optimization NW is the study chairman of RiSK, developed the design of the registry study, is involved in continuing optimization and helped to draft the ... function following pelvic irradiation in prepubertal, pubertal and young adult women Strahlenther Onkol 2005, 181:534-539 Tarbell N, Guinan E, Chin L, Mauch P, Weinstein H: Renal insufficiency ... coordinator of RiSK, participated in the development of the study trial and drafted the manuscript AS, HP, CR, BP, BT, RDK and KD participated in the design of the registry study and are involved...
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Báo cáo sinh học: "Optimal design for the detection of a major gene segregation in crosses" potx
... to the ’segregating groups’ increased between C10 and C19, C20 and C26, and C3 and C5 The proportion of F2s to backcrosses increased between C30 and C35, C40 and C44, and C50 and C54 The major ... major gene for rapid postweaning growth in mice Genet Res Camb 44, 293-308 Dickerson GE (1973) Inbreeding and heterosis in animals In: Proc Anim Breeding Genet P Sym in honour of Dr JL Lush, Am ... changes in curve shape with the gene effect a must be emphasized These curves are nearly linear for power under 70% and, in this linear part, the slope (ie the gain in power = = = per extra individual...
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