mass spectrometry

Determination of EDTA in Water Samples by SPE-Gas Chromatography/Mass Spectrometry

Ngày tải lên : 05/09/2013, 10:15

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Tài liệu Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry pdf

Ngày tải lên : 14/02/2014, 08:20

... Commun. Mass Spectrom. 2008; 22: 2993–2998 DOI: 10.1002/rcm 2994 L. Zhu et al. Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry Liang ... fast response. Mass spectrometry-based methods are of particular interest for the on- line analysis of reactions, 1 due to their high sensitivity and high speciﬁcity. Tandem mass spectrometry ... organic chemistry. 2,3 Although direct infusion electrospray ionization spectrometry (ESI-MS) 4–9 and membrane introduction mass spectrometry (MIMS) 10–12 are gaining popularity in this ﬁeld, both...

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$Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic proﬁles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx$

Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic proﬁles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx

Ngày tải lên : 16/02/2014, 15:20

... Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics 4, 793–811. 5 Roblick UJ, Hirschberg D, Habermann JK, Palmberg C, Becker ... colorectal cancer by com- bining two-dimensional polyacrylamide gel electrophoresis with a strictly mass spectrometry- based approach for data analysis. Mol Cell Proteomics 5, 2092–2101. 9 Kim H, Kang ... eukaryotic organisms. Protein Sci 7, 1029–1038. 12 Wu CC & Yates JR III (2003) The application of mass spectrometry to membrane proteomics. Nat Biotechnol 21, 262–267. 13 Bisle B, Schmidt A, Scheibe...

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Tài liệu Báo cáo khoa học: A functional polymorphism of apolipoprotein C1 detected by mass spectrometry docx

Ngày tải lên : 19/02/2014, 05:20

... peptide mass ﬁngerprint from MALDI-TOF MS showed m ⁄ z values corresponding to all eight theoretical peptides plus the peptide of the truncated protein (residues 1–13, TPDVSSALDKLKE, theoretical mass ... reﬂectron mode and in a QSTAR o-MALDI mass spectrometer (Applied Biosystems Inc., Bellarica, CA, USA). MS ⁄ MS analysis was conducted with the QSTAR o-MALDI mass spectrometer. The TOF region acceleration ... National Hemophilia Clinical Fellow- ship Award. KM is a Bush Medical Fellow (Bush Foundation). The mass spectrometry and parts of the gel electrophoresis were conducted with the assistance of Mr Thomas...

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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

Ngày tải lên : 19/02/2014, 12:20

... MS. The measured molecular mass of the puriﬁed product was 11 799.70 ± 2, which concurs closely with activated (Pyr)-ONC (M23L), which has a theoretical molecular mass of 11 801.60. Contribution ... soon as the samples were dry, the probe was transferred into the mass spectrometer so that spectra could be acquired immediately. The mass spectra for the same samples were acquired again, 24 h and ... of the peaks corresponding to Gln-ONC (M23L) (theoretical molecular mass 11 818.87) and Pyr-ONC (M23L) (theoretical molecular mass 11 801.60), and expressing it as the fraction of hydrolyzed (Met1)-ONC...

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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc

Ngày tải lên : 19/02/2014, 12:20

... com- bined MS/MS of singly and doubly charged molecular ions in negative-ion electrospray mass spectrometry. J. Am. Soc. Mass Spectrom. 13, 670–679. 15. Domon, B. & Costello, C.E. (1988) A systematic ... °Cat10°CÆmin )1 . ES-MS Negative-ion ES-MS and CID MS/MS were carried out on a Micromass (Manchester, UK) Q-Tof mass spectrometer. Nitrogen was used as desolvation and nebuliser gas at a ﬂow rate ... contaminant in DFiLNO as the precursor (B). 1176 H. Kogelberg et al.(Eur. J. Biochem. 271) Ó FEBS 2004 mass spectrometry and 1 H-/ 13 C-nuclear magnetic resonance spectroscopy. Evidence for a new core...

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Tài liệu Báo cáo khoa học: Si-face stereospeciﬁcity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry ppt

Ngày tải lên : 20/02/2014, 03:20

... stereospecificity at C5 of coenzyme F 420 for F 420 H 2 oxidase from methanogenic Archaea as determined by mass spectrometry Henning Seedorf 1 ,Jo ¨ rg Kahnt 1 , Antonio J. Pierik 2 and Rudolf K. Thauer 1 1 ... nicotinamide adenine dinucleotide-linked oxidoreductases: electron impact and field desorption mass spectrometry. Anal Biochem 101, 413–420. 22 Warkentin E, Mamat B, Sordel-Klippert M, Wicke M, Thauer ... (5R)- [5- 2 H 1 ]F 420 H 2 to [5- 2 H]F 420 as to be expected for a Si-face-specific enzyme. The finding that the 775 Da mass peak in Fig. 2Cb was much lower than in Fig. 2Bb is probably due to the fact that reduction of...

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TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx

Ngày tải lên : 05/03/2014, 21:20

... Techniques for the Analysis of Organic Chemicals by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Petrochemical Authors Ed McCurdy & Don Potter Agilent Technologies ... Cheadle Royal Business Park Manchester, SK8 3GR UK Abstract Inductively Coupled Plasma Mass Spectrometry (ICP- MS) is used for the routine monitoring of trace and ultra-trace metal contaminants ... technique is available to any Agilent ICP-MS system fitted with the ShieldTorch System and a mass flow controller capable of adding oxygen to the plasma. In routine operation, automatic switching...

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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot

Ngày tải lên : 07/03/2014, 17:20

... soft ionization mass spectrometry. Int J Mass Spectrom 216, 1–27. 54 Heck AJR & van den Heuvel RHH (2004) Investigation of intact protein complexes by mass spectrometry. Mass Spectrom Rev 23, ... (1998) Fourier transform ion cyclotron resonance mass spectrometry: a primer. Mass Spectrom Rev 17, 1–35. 56 Smith RD (2000) Evolution of ESI -mass spectrometry and Fourier transform ion cyclotron ... interconversion of the two most common frequency-to -mass calibration functions for Fourier transform ion cyclotron resonance mass spectrometry. Int J Mass Spectrom 195 ⁄ 196, 591– 598. 83 Otwinowski...

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Báo cáo khoa học: Systematic quantiﬁcation of complex metabolic ﬂux networks using stable isotopes and mass spectrometry pptx

Ngày tải lên : 08/03/2014, 08:20

... least reliable mass isotopomer fractions were considered in the flux analysis. Discussion In the work described, we examined the extent to which mass isotopomer measurements of biomass hydrolysates, after ... flux networks using stable isotopes and mass spectrometry Maria I. Klapa*, Juan-Carlos Aon† and Gregory Stephanopoulos Department of Chemical Engineering, Massachusetts Institute of Technology, ... M.I. (2001) High resolution metabolic flux quantification using stable isotopes and mass spectrometry. PhD Thesis, Massachusetts Institute of Technology, Cambridge, MA, USA. 8. Wiechert, W., Mollney,...

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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc

Ngày tải lên : 15/03/2014, 00:20

... mass spectrometry for protein interaction analysis: application to a calmodu- lin ⁄ target peptide complex. Anal Chem 77, 495–503. 19 Tang XT, Munske GR, Siems WF & Bruce JE (2005) Mass spectrometry ... stored at )20 °C until MS analysis. High mass MALDI-TOF MS High -mass MALDI-TOF mass spectra of the intact protein complexes were obtained using a MALDI-TOF mass spectrometer (Voyager DE STR; Applied ... 000 98 000 132 000 166 000 200 000 Intensity (%) 100 50 0 Mass (m/z) Mass (m/z) Counts Counts Counts A B C Fig. 2. High -mass MALDI-TOF mass spectra of the products generated upon cross-linking....

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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx

Ngày tải lên : 16/03/2014, 03:20

... nanoflow electrospray ionization orthogonal time-of-flight mass spectrometer (Micromass LC-T; Waters, Manchester, UK) modified for high mass operation and operating in positive ion mode [32]. To monitor ... described previously [31]. Three independent values were obtained for each strain. Macromolecular mass spectrometry MS studies were carried out in aqueous ammonium acetate (100 mm, pH 7.0). Kid:Kis ... These protein complexes are detected at different mass- to-charge ratios ( m ⁄ z), separated by differences in their time-of-flight inside the mass analyser. Here, we use this new powerful technology...

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Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx

Ngày tải lên : 16/03/2014, 03:20

... derivative. AT ACP-TE Methyl- malonyl adduct Unloaded forms Methyl- malonyl adducts Unloaded form adduct 33 200 32 80032 400 39 500 39 600 39 70039 400 Mass (Da) Mass (Da) Fig. 3. The electrospray mass spectra produced by the AT and ACP-TE fractions derived from the extension ... Transform mass spectrometry: The polyketide module of yersiniabactin synthetase. Biochemistry 42, 13393–13400. 33 Hicks LM, O’Connor SE, Mazur MT, Walsh CT & Kelleher NL (2004) Mass spectrometric ... ionization Fourier-Transform mass spectrometry. Nat Prod Rep 23, 893–918. 35 Bumpus SB & Kelleher NL (2008) Accessing natural product biosynthetic processes by mass spectrometry. Curr Opin Chem...

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Báo cáo khoa học: Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry doc

Ngày tải lên : 16/03/2014, 18:20

... transform mass spectrometry: The polyketide module of yersiniabactin synthetase. Biochemistry 42, 13393–13400. 17 Hicks LM, O’Connor SE, Mazur MT, Walsh CT & Kelleher NL (2004) Mass spectrometric ... identity of each fragment was obtained by mass mapping from the known amino acid sequence. Given the good mass accuracy (0.01%), for any particular observed mass a match could normally be found with a ... for catalytic and structural studies. In our work, ion trap mass spectrometry was used. Compared with the Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometer [16,17], the ion trap is less...

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DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf

Ngày tải lên : 22/03/2014, 14:20

... capillary column of a gas chromatography /mass spectrometry (GC/MS) system. Compounds eluting from the GC column are identified by comparing their measured mass spectra and retention times to reference ... quadrupole mass spectrometer. Table 8 lists accuracy and precision data from replicate determinations of method analytes in tap water using liquid-solid cartridge extractions and the ion trap mass ... and output mass spectral data. The computer software must have the capability of processing stored GC/MS data by recognizing a GC peak within any given retention time window, comparing the mass spectrum from...

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Ionization Methods in Organic Mass Spectrometry pot

Ngày tải lên : 22/03/2014, 14:20

... the right conditions. For such compounds, negative ion mass spectrometryis more efﬁcient, sensitive and selective than positive-ion mass spectrometry. Negative ions can be produced by a number ... polar or basic compounds . permits the detection of high -mass compounds at mass- to-charge ratios that are easily determined by most mass spectrometers (m/z typically less than 2000 to 3000). . ... electron energy to be precisely controlled for negative-ion mass spectrometry, leading to more sensitive, selective, and reproducible mass spectra for environmentally important compounds. . efﬁcient...