mass spectrometry and infrared spectroscopy
Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... Conformational studies on the selectin and natural killer cell receptor ligands sulfo- and sialyl-lacto-N-fucopentaoses (SuLNFPII and SLNFPII) using NMR spectroscopy and molecular dynamics simulations ... pattern, sequence, and partial linkages, and assign structural details of each branch (e.g the 3- and 6-branches) Blood group H, Lea/Lex and Leb/Ley determinants, together with type and type chains, ... pattern and sequence analysis of underivatized oligosaccharides by combined MS/MS of singly and doubly charged molecular ions in negative-ion electrospray mass spectrometry J Am Soc Mass Spectrom...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... cyclotron resonance mass spectrometry: a primer Mass Spectrom Rev 17, 135 56 Smith RD (2000) Evolution of ESI -mass spectrometry and Fourier transform ion cyclotron resonance for proteomics and other biological ... using soft ionization mass spectrometry Int J Mass Spectrom 216, 127 54 Heck AJR & van den Heuvel RHH (2004) Investigation of intact protein complexes by mass spectrometry Mass Spectrom Rev 23, ... for mass spectrometry of large biomolecules Science 246, 6471 52 Loo JA (2000) Electrospray ionization mass spectrometry: a technology for studying noncovalent macromolecular complexes Int J Mass...
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Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx
... patients confidence and quantitation accuracy for proteomic research, especially for low-abundance proteins [16,17] We used iTRAQ labeling together with gel-assisted digestion and mass spectrometry ... membrane fractions from tumoral and adjacent normal mucosa from colorectal cancer patients Differentially expressed proteins were identified by mass spectrometry and verified by western blotting ... across the eight patients Proteins identified by mass spectrometry were classified by subcellular location and molecular function (Fig 3) To better understand the probable roles of the membrane proteomes...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... contribution of Met1, and Met23 substituted for Leu, Fig Comparison of the cyclization reaction as a function of temperature and pH (A), (B) and (C): (d), (s) and (.) correspond to pH 2.5, 8.0 and 11.5, ... hydrolysis reaction as a function of denaturing agent and pH (A), (B) and (C): (d), (s) and (.) correspond to guanidinium chloride concentrations of , and M, respectively Hydrolysis was calculated as ... (M23L) signal in the MALDI-TOF mass spectra h after the addition of AAP The pH was adjusted to 2.5, 8.0 and 11.5 and the samples were incubated at 37 °C, 50 °C and 70 °C Aliquots were taken at...
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Tài liệu Infrared Spectroscopy – Life and Biomedical Sciences Edited by Theophile Theophanides pptx
... Chapter 12 Vis/Near- and Mid- Infrared Spectroscopy for Predicting Soil N and C at a Farm Scale Haiqing Yang and Abdul M Mouazen 185 Chapter 13 The Application of Near Infrared Spectroscopy for the ... value of NIRS and its usefulness in leading to timely interventions and prevention of long term injury (www.ninds.nih.gov/news_andevents/proceedings/ Infrared Spectroscopy – Life and Biomedical ... improve life and care in the NICU for patients and staff? Perhaps Possibly clinicians find themselves confronted by unexpected physiology and new problems 16 Infrared Spectroscopy – Life and Biomedical...
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Tài liệu Báo cáo khoa học: Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells doc
... assigned to the asymmetric stretching of CH3, and the band at 2873 cm)1 to its symmetric mode The bands at 2926 and 2853 cm)1 can be assigned to an asymmetric and symmetric stretching mode of CH2, respectively ... between 1300 and 900 cm)1 arise mainly from phosphate associated with nucleic acids, i.e DNA and RNA The absorption bands at 1245 and 1087 cm)1 are characteristic of asymmetric and symmetric ... (1999) infrared spectroscopy of cells and tissues: shining light on a novel subject Appl Spectrosc 53, 148A–161A 16 Wong, P.T., Wong, R.K., Caputo, T.A., Godwin, T.A & Rigas, B (1991) Infrared spectroscopy...
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Báo cáo khoa học: Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry pptx
... structurally observable from the mass isotopomer distribution of lysine, fragments [1-4] and [2-4] of OAA and [1-3] and [2-3] of PYR [7], the obtained measurements of these mass isotopomer fractions ... carbons and and to a lesser extent of and of lysine that enables the estimation of the flux ratio at H4D using NMR measurements Finally, the irreversibility between the PEP and the PYR and OAA ... Table The elemental composition and ash content of biomass were considered to be C3.97H6.46O1.94N0.845 and 3.02%, respectively [32] Trehalose, acetate, lactate and alanine were included in the...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... 1–7) and His-Tagged Ssa1p (lanes 8–14); a mixture of untreated Ure2p and Ssa1p is seen in lanes and 10 Ure2p, Ssa1p and Ure2p incubated with Ssa1p treated with BS2G are seen in lanes and 8, and ... treated with BS2G are seen in lanes and 8, and 11 and and 13, respectively Similar samples treated with BS3 are seen in lanes and 9, and 12 and and 14, respectively The arrows show the cross-linked ... protein bands resolved by SDS ⁄ PAGE and, corresponding to monomeric Ure2p, monomeric Ssa1p and Ure2p–Ssa1p complexes with apparent molecular masses of 120 and 160 kDa were excised Each protein band...
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Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot
... number and position, as well as an estimation of the bandwidth and the intensity of the bands making up the amide I or I¢ region Thereafter, curve fitting was performed and the heights, widths and ... Structure and thermal denaturation of crystalline and noncrystalline cytochrome oxidase as studied by infrared spectroscopy Biochemistry 33, 11650–11655 Krimm S & Bandekar J (1986) Vibrational spectroscopy ... Corbalan-Garcı´ a S & Gomez-Fernandez JC (2003) Structural study of the C2 domains of the classical PKC isoenzymes using infrared spectroscopy and two-dimensional infrared correlation spectroscopy Biochemistry...
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Báo cáo khoa học: Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry doc
... ATLACPL and KS1 but not onto AT1, KR1, ACP1 or TE The loading module fragment also showed two peaks, LM1 and LM2 with mass increase of 67 and 137 Da (theoretical mass increase of 70 and 140 Da, ... the number of digestion fragments and resulted in the released domains being readily separable by a reverse phase C4 column and monitored by on-line UV and mass spectrometry Our choice of conditions ... acyl-intermediates on ATL-ACPL and KS1 demonstrates that the formed acyl-enzyme intermediates are stable under the digestion and analytical conditions used here, and the ion trap mass spectrometry used is...
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DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf
... threshold 441 Present and < Mass 443 High -mass resolution 442 Base peak or >50% of Mass 198 High -mass resolution and sensitivity 443 15-24% of Mass 442 High -mass resolution and isotope ratio All ... peak Low -mass sensitivity 68
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MASS SPECTROMETRY IN DRUG METABOLISM AND DISPOSITION docx
... Lee, and Guodong Chen Characterization of Impurities and Degradants Using Mass Spectrometry Mike S Lee and Mingshe Zhu Mass Spectrometry in Drug Metabolism and Disposition: Basic Principles and ... Dai, Jiuming He, and Jian Liu 19 Liquid Chromatography Mass Spectrometry Bioanalysis of Protein Therapeutics and Biomarkers in Biological Matrices 613 Fumin Li and Qin C Ji 20 Mass Spectrometry in ... applications of imaging mass spectrometry for these studies are described Novel instrumentation and mass spectrometry scan functions of hybrid triple quadrupole–linear ion trap mass spectrometry are...
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CHARACTERIZATION OF IMPURITIES AND DEGRADANTS USING MASS SPECTROMETRY pot
... Lee, and Guodong Chen ț Characterization of Impurities and Degradants Using Mass Spectrometry Mike S Lee and Mingshe Zhu ț Mass Spectrometry in Drug Metabolism and Disposition: Basic Principles and ... an ion and a neutral in free space has the center-of -mass (COM) energy ECOM ECOM ¼ mi mn v mi þ mn R ð1:22Þ TANDEM MASS SPECTROMETRY 31 where mi is the ion mass, mn is the neutral mass, and n ... Chromatography Mass Spectrometry 1.5.2 Liquid Chromatography Mass Spectrometry 1.5.3 Capillary Electrophoresis Mass Spectrometry 1.5.4 Ion Mobility Spectrometry Mass Spectrometry 7 10 10 14 19 22 25 28 29 30 32...
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Tandem Mass Spectrometry – Applications and Principles Edited by Jeevan K. Prasain docx
... reaction enthalpies, proton and ion affinities, gas-phase acidities, and so on 4 Tandem Mass Spectrometry – Applications and Principles Mass spectrometry is extremely versatile and several areas of ... Introduction 1.1 Mass spectrometry brief history and recent developments The first and most important question to be asked: What is mass spectrometry? The basic principle of mass spectrometry (MS) ... Tandem Mass Spectrometry of Tagged and Permethylated Polysaccharides 285 Wen-Bin Yang Part Metabolite Identification and Quantification 307 Chapter 14 Metabolomics Research with Tandem Mass Spectrometry...
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mass spectrometry of proteins and peptides
... Winston, S., and Hauer, C R (1986) Protein sequencing by tandem mass spectrometry Proc Natl Acad Sci USA 83, 6233–8238 Yost, R A and Boyd, R K (1990) Tandem mass spectrometry: quadrupole and hybrid ... tandem mass spectrometer, in Proceedings 46th ASMS conference on Mass Spectrometry and Allied Topics, Orlando, FL, p 237 24 Lingner, J., Hughes, T R., Shevchenko, A., Mann, M., Lundblad, V., and ... Yates, III, Andrew J Link, and David Schieltz Introduction Tandem mass spectrometry is a powerful mixture analysis technique suitable for sequence analysis of peptides (1) A tandem mass spectrometer...
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protein and peptide analysis by mass spectrometry
... Resonance Mass Spectrometry VCH, Wemheim, Germany 15 Busch, K L., Glish, G L., and McLuckey, S A (1988) Mass Spectrometry /Mass Spectrometry Technzques and Applications of Tandem Mass Spectrometry ... Biemann, K and Scoble, H A (1987) Characterization by tandem mass spectrometry Science 231,992-998 18 Gtbson, B W and Cohen, P (1990) Ltquid secondary ion mass spectrometry of phosphorylated and sulfated ... decomposttton m a tandem mass spectrometer: differentiatton of leucme and tsoleucme Anal Chem 59,262 12 Biemann, K (1990) Sequencmg of peptides by tandem mass spectrometry and high-energy colhsion-induced...
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Báo cáo sinh học: "Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" pot
... reflector mode Ten tandem mass spectra were recorded from each spot Ions with a signal to noise (S/N) ration greater than 30 were chosen for tandem mass spectrometry The tandem mass spectra were ... analysis, analyzed the mass spectrometry data and helped to draft the manuscript SV assisted with the analysis of the mass spectrometry data DEH and CSM coordinated the research efforts and edited the ... subjected to trypsin digestion, and analyzed by the LC-ESI-Q-TOF mass spectrometer The full scan mass spectrum displays a peak at m/z 844.5 (inset), and corresponding tandem mass spectrum identifies...
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báo cáo hóa học: " Relationship between oxygen supply and cerebral blood flow assessed by transcranial Doppler and near – infrared spectroscopy in healthy subjects during breath – holding" doc
... He showed aphasia, motor impairment, and poor scores in fluency and verbal tests After a year of drug therapy (antihypertensive and antiaggregating agents) and logopedic therapy, this subject ... motor control and reaching tasks, and increased his AAT (Aachener Aphasie Test) score from 52/60 to 56/60 Measurement protocol We applied TCD and NIRS during baseline conditions and during CO2 ... filter, with ripple in the stop band, cutoff frequency equal to 50 mHz and at least 30 dB of discrimination) and set the initial concentrations equal to zero The O2Hb and CO2Hb concentration signals...
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báo cáo hóa học: " Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry" doc
... internal standard and b) without internal standard Figure the corresponded product the deuterated standards with Standard chromatogram of ion scans Standard chromatogram of the deuterated standards ... Leukotrienes LTB4 and LTE4as analytical standards and the labelled [2H4] LTB4 and [2H4] PGE2 as internal standards were purchased from Cayman Chemicals Company (Michigan, USA) All analytical standards ... specificity and detection related to structural information such as mass spectrometry Our developed method allows for a sensitive, specific and reliable determination of leukotriens and prostaglandins...
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