manipulation of gene expression in the central nervous system with lentiviral vectors
Chemical drug assisted gene transfer a sensible approach to improve transgene expression in the central nervous system
... used in each injection The rate of injection was μl/min and the needle was allowed to remain in situ for mins before being slowly retracted at the end of each injection The intrathecal lumbar injection ... biodegradable linkages as well as designing the degree of cross-linking, the particle size and the surface charge of the resulting conjugates In addition, there is little information on using functional ... viral vectors still make viral vectors the dominant delivery vehicles in gene therapy field There 17 Chapter Introduction are a lot of viral vectors being utilized for delivering genes to the CNS;...
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báo cáo khoa học: "In vivo observation of gold nanoparticles in the central nervous system of Blaberus discoidalis" potx
... by the nAuNPs The XPS was used to verify the presence of nAuNPs embedded in the brain tissue The confocal microscope and TEM were used to gain insights into the interaction of nAuNPs and the insect’s ... to the CNS The nAuNPs were introduced in the CNS through an injection between the brain and the sub esophageal ganglion (SEG) through the neck in the direction shown in Figure A cc syringe with ... changes in noise and vibration in the building Although proteins not break into ions, introducing charged particles into the nervous system causes an imbalance in the signal transmission that links...
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The role of hydrogen sulfide in the central nervous system implications in the treatment of alzheimers disease
... like tau is involved in microtubule assembly It serves as a binding for the tubulin Mutations in α-synuclein lead to loss of this binding ability, resulting in tubulin and α-synuclein aggregation ... inhibit the production of Aβ or escalate its clearance in the brain The most common mechanisms of action of these drugs are the inhibition of the rate limiting steps of Aβ generation i.e β- and/or ... improved the survival rate of hippocampal neurons thus reducing the learning and memory impairment in the rats with induced ischemic stroke H2S increased the phosphorylation of Akt while inhibited the...
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INVESTIGATION OF MEMBRANE PROPERTIES IN THE CENTRAL NERVOUS SYSTEM OF DROSOPHILA MELANOGASTER STUDIED BY FLUORESCENCE CORRELATION SPECTROSCOPY
... constituent instead of sphingomyelin (PE-cer has phosphoethanolamine as the head group, instead of the phosphocholine head group in SM) [32] Despite having shorter fatty acyl chains (the longest being ... measurements of a single type of fluorescent probe with one in the membrane plane and the other as internalized vesicles As the nucleuses of motor neurons are generally large, internalized vesicles in the ... due to tissue interposed between the lens and the cell of interest (b) Enlarged schematic diagram showing the arrangement of aCC/RP2 motor neurons within the central nervous system of stage-16...
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báo cáo hóa học: " Temporal expression and cellular origin of CC chemokine receptors CCR1, CCR2 and CCR5 in the central nervous system: insight into mechanisms of MOG-induced EAE" pptx
... around inflammatory foci in the spinal cord of rats with EAE, thus identifying potential determinants for trafficking of these cells to the CNS These findings are discussed in relation to therapeutic ... apoptosis of infiltrating cells in the CNS of animals with EAE [80] The limitation of inflammation seen in the CNS could also be the result of a diminished antigen-presenting capability In conclusion, ... with marked demyelination in the inflammatory areas (F) The majority of the infiltrating inflammatory cells are macrophages, as evidenced by positive staining for the ED-1 marker (E) During the...
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Báo cáo y học: "Cell-specific microarray profiling experiments reveal a comprehensive picture of gene expression in the C. elegans nervous system" ppsx
... the majority of genes in this interactome group are expressed in the nervous system favors the idea that these networks reflect authentic interactions in neurons We note that 13 of the proteins ... number of genes in a given neuron and are, therefore, incomplete To test the applicability of the mRNA-tagging strategy for obtaining a comprehensive gene expression profile of a specific subset of ... function in neurons [21] Expression of the additional synaptotagmin genes in the nervous system may account for the residual synaptic vesicle function of snt-1 mutants [21] Three members of the copine...
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Báo cáo sinh học: "Systemic 5-fluorouracil treatment causes a syndrome of delayed myelin destruction in the central nervous system" doc
... at the month time point, along with markedly decreased levels of MBP in the CC and in the white-matter tracts of the striatum of treated animals (Figure 10) In agreement with the majority of the ... demyelinated axon with rarefaction of the axoplasm (asterisk) and two axons with collapsed centers and inter-laminar splitting of the myelin sheaths (arrows) can be seen, indicating on-going myelin ... displayed scattered foci of demyelinated axons, including partial or complete loss of myelin sheaths and increases in inter-laminar splitting of the myelin sheaths Analysis of transverse sections...
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Báo cáo khoa học: "Immunohistochemical study of constitutive neuronal and inducible nitric oxide synthase in the central nervous system of goat with natural listeriosis" potx
... expression in the goat brain with listeriosis The involvement of iNOS in bovine and caprine listeriosis has been reported previously, and the increased expression of iNOS and the resultant NO generation ... monocytogenes antisera were observed in inflammatory microabscesses Counterstained with hematoxylin Magnification: ×132 C: Immunohistochemical staining of inducible NOS in the caprine brain with ... single positive-staining bacteria were found in the neuronal processes Both nNOS and iNOS immunoreactivity were found in a proportion of the neurons in the brainstem and the staining pattern was...
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Báo cáo y học: "Mouse maps of gene expression in the brain" pot
... consistent, indicating that the pattern is due to the promoter elements of the gene of interest rather than the genomic insertion point The anatomical annotators of the GENSAT data have embraced the ... protein (EGFP) reporter gene The BAC is then used to create a transgenic mouse line that ideally expresses EGFP in the cells that normally express the gene of interest The advantage of using the ... through the Mutant Mouse Research Resource Centers [8,9] In addition, for some of the most interesting expression patterns, the project is developing lines where the BAC containing the gene promoter...
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Báo cáo y học: "The LeFE algorithm: embracing the complexity of gene expression in the interpretation of microarray data" pps
... basis, the random forest determines the importance score of each gene (variable) in the multivariate model The distribution of importance scores of the genes in the category is then compared with the ... value of the training samples in the case of continuous variables The aggregate forest's output is then determined by averaging the regression values of the trees or using a weighted voting process ... applications of LeFE, to gene expression in three breast cancer classes and to in vitro gefitinib sensitivity (see Results), provide further proofs of principle The findings highlight the distinctions...
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Báo cáo y học: "The LeFE algorithm: embracing the complexity of gene expression in the interpretation of microarray data" potx
... SLA2/Huntingtin-interacting protein Hip1 Y66H1B.3 462 784 Actin-binding cytoskeleton protein, filamin Y66H1B.2 119 902 Actin-binding cytoskeleton protein, filamin 766 20 Actin-binding protein Coronin, contains WD40 ... Actin regulatory proteins (gelsolin/villin family) Y71G12B.11 122 Talin W03F11.6 afd-1 - 393 Actin filament-binding protein Afadin F08A8.6 tag-138 - 474 Actin-binding protein SLA2/Huntingtin-interacting ... 24 genes encoding proteosome subunits show elevated expression in the M24 dataset whereas none of these transcripts are enriched in the M0 profile This finding could be indicative of the general...
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Genetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system
... functions of the therapeutic genes 1.1.2 Non-viral and Viral Gene Delivery Systems Depending on the usage of vectors, there are two main types of gene delivery systems: non-viral and viral systems ... However, the low efficiency of non-viral gene delivery remains a critical drawback of the system On the other hand, viral gene delivery systems are favoured because of the high efficiency of infective ... 1998) Even in the presence of peripheral infection with adenovirus, there is virtually no immune response in the brain following direct injection of “gutless” vectors in rats However, the high...
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A study of the recombination activating gene 1 in the zebrafish nervous system
... regulation of Rag genes 1.2 Rags in the nervous system 1.2.1 The expression of Rag genes in the nervous system Rag genes were initially identified as lymphoid-specific factors Indeed, Rag genes are ... the expression of Rag1 in the nervous system, and its function remains unknown As a step towards understanding the role of Rag1 in vertebrate nervous system, we have initiated a study using the ... nervous system 13 1.2.1 The expression of Rag genes in the nervous system 13 1.2.2 A brief overview of the nervous system 16 1.2.3 Questions about the neuronal function of Rag1 17 1.3 Advantages of...
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Development of hybrid promoters and viral vectors for improved gene delivery to the central nervous system
... cells in the nervous system, carrying out the fundamental tasks of receiving, conducting and transmitting signals Therapeutic protection of these cells is one of the main goals of gene therapy of ... Chiocca, 2000) The difficulty for gene therapy of glioma tumors is how to restrict the delivered therapeutic gene within the tumor cells, since the expression of the therapeutic genes in normal cells ... recurrent brain tumors, or to inject drugs with short half-life Gene therapy, on the other hand, can minimize this kind of damage Chapter Introduction to the brain by a single delivery of genetic...
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báo cáo khoa học: "Isolated angiitis of the central nervous system with tumor-like lesion, mimicking brain malignant glioma: a case report and review of the literature" pdf
... provided the pathological diagnosis TJ was the surgeon and gave the final approval of the version to be published Competing interests The authors declare that they have no competing interests ... conditions, the potential for neurological recovery, et al) In our case, the initial pulse therapy with steroid failed to prevent the rapid progress of vasculitis Only combined therapy with cyclophosphamide ... China Department of Pathology, Beijing Neurosurgical Institute, Beijing 100050, China Authors’ contributions GY wrote the initial draft WY drew the pictures WZ revised the draft SL described the...
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báo cáo hóa học:" Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs" pot
... match the surface area of the condyle The areas of India ink staining were outlined using a permanent marker Tracings of the India ink-stained tibial and femoral condyles were evaluated without ... assessments The lamina splendens was not disrupted in any location based on lack of India ink staining, indicat- ing that AC surface integrity was maintained for two weeks in the dogs in this study ... level of expression for each gene (± standard error) compared to the house keeping gene GAPDH The increase in ADAMTS gene expression in the CrMFC approached significance and was included in the...
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Báo cáo toán học: "Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line: Evidence for an Anti-Estrogen Effect of Iodine" doc
... of current therapies In this study we provide the first gene array profiling of an estrogen responsive breast cancer cell line demonstrating that the combination of iodine and iodide alters gene ... through increasing 2-methoxyestradiol, decreasing 3, 4-estradiol quinone or indirectly via the inactivation of E2 The importance of the CYP1A1/CYP1B1 ratio in- vivo is evident in the increased ... the body of evidence builds, the importance of iodine on the maintenance of healthy breast tissue and its role in carcinogenesis becomes clearer Unveiling iodine’s mechanism of action is of crucial...
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Curcumin modulates dopaminergic receptor, CREB and phospholipase c gene expression in the cerebral cortex and cerebellum of streptozotocin induced diabetic rats potx
... hrs after the administration of morning dose of insulin and curcumin The results were expressed in terms of milligram per decilitre of blood Total Dopamine receptor binding studies in the cerebellum ... interest in learning dopamine D2 receptor expression begins with the hypothesis that dopamine D2 receptors are involved in the pathophysiology of schizophrenia and in the mechanism of antipsychotic ... mRNA expression to near control Such interference with the dopaminergic system could explain, at least in part, the ameliorative effect of curcumin on CNS In agreeable with the total dopamine...
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báo cáo khoa học: " Identification of microspore-active promoters that allow targeted manipulation of gene expression at early stages of microgametogenesis in Arabidopsis" pps
... gametophytic expression in developing microspores Mutations in the Arabidopsis TWO -IN- ONE (TIO) protein kinase result in binucleate pollen grains due to the failure of cytokinesis in microspores ... Verification of microarray gene expression data by RT-PCR Verification of microarray gene expression data by RT-PCR The expression of three genes selected for further GUS expression assays was examined in ... specifically expressed in anthers with the exception of MSP1 that also showed limited expression in the distal tips of cotyledons and true leaves Within developing flowers their expression was restricted...
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Báo cáo y học: "New insights into the pathogenesis of glucocorticoid-induced avascular necrosis: microarray analysis of gene expression in a rat model" potx
... than other endothelial cells in the body In keeping with the theory of endothelial cell activation having a role in ANFH, coagulation-related gene expression in particular serine (or cysteine) ... immunohistochemical findings for the two other genes of interest (COL2A1 and MIA) failed to show enhanced protein expression Discussion The early events in the pathogenesis of ANFH are incompletely understood ... development of early steroid-induced ANFH Determination of whether A2M over -expression in our study is either the result or the cause of the apoptosis found in our rats developing early ANFH following...
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