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progress in botany. 73 [electronic resource]
Ngày tải lên :
29/05/2014, 23:58
... and C2, and three interfaces, a (between the external medium and C1), b (between C1 and C2) and c (between C2 and the internal medium) Interface a was considered a membrane permeable to Na+ and ... With indices k and j referring to K+ and Rb+, and using the radioactive isotopes 40K and 86Rb as tracers, we have measured the fluxes, Jk(ce ) and Jj(ce ) [8] Applying equations (30) and (31) to ... cytoplasm, c, and the vacuole, v; the membranes 20 M Thellier are the plasmalemma (between w and c) and the tonoplast (between c and v) The relative volumes of compartments w, c and v and the surface...
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Báo cáo khoa học: Protein–protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling doc
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06/03/2014, 11:20
... fused to protein X and Ste2p receptor fused to protein Y permitted pheromone response signaling through the contact between Ste2p and Gpa1p–Gas, using the interaction between Snf1 and Snf4, which ... example, interactions of attractive drug target candidates, syntaxin 1a and nSec1 or fibroblast-derived growth factor receptor and SNT-1, were monitored, and nSec1 mutants that lost the ability to bind ... neurotransmission, and cell growth [29] Yeast has only two types of heterotrimeric G-protein: pheromone signalingrelated and nutrient signaling-related [30–32] Nutrient signaling is profoundly and intricately...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
Ngày tải lên :
06/03/2014, 11:20
... CDRs of heavy chain and CDR3 of light chain [ref 39] Fv Randomization in CDR loops of heavy and light chains Y/S library in BC, DE and FG loops 10 FN3 Randomization in BC, DE and FG loops Fig Local ... imaging [5], and proteomics [6,7] The presence of the vast gene cluster enables us to obtain valuable binding proteins using selection methodology, and recent structural visualization of candidate ... (XGGGS)n in which the X residues were randomized and the linker length (n) was intermittently varied (B) DARPin: six amino acids in the loop and helix structures are randomized (C) A-domains: variable...
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Báo cáo y học: "Natural selection of protein structural and functional properties: a single nucleotide polymorphism perspect" potx
Ngày tải lên :
14/08/2014, 08:21
... contributions ZZ and JL designed the study JL and YZ collected the data and performed the data analysis XL participated in the statistical analysis JL and ZZ drafted the manuscript All authors read and approved ... 0.26] for cytoplasmic proteins, and [0.26, 0.29] for membrane proteins transporter activity' and 'motor activity', and the opposite groups are 'receptor activity' and 'isomerase activity' (Figure ... regions [43], and that they are more likely to be involved in regulatory functions and protein-protein interactions [44,45] We obtained disorder predictions using DISOPRED2 [43] and retained only...
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Development of a novel toll like receptor based two hybrid assay for detecting protein protein interactions and its application in the study of CD14 dimerization and FcyRIIA activation
Ngày tải lên :
12/09/2015, 08:20
... and IKKε/IKK showed impaired activation of IRF-3 and expression of IFN-β and IFN inducible genes in response to TLR3 and TLR4 ligands (McWhirter et al., 2004; Hemmi et al., 2004) Thus, TBK1 and ... of the TIR domains for TLR1, TLR2 and TLR2 mutant The β-strands or α-helices are given alphabetical denotations For example, βA and αA are the first β-strand and the first α-helix, respectively ... research scholarship and for giving me the opportunity to work here Last, but not least, my deepest love and appreciation to my husband and my family members for their love, care and support Without...
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Báo cáo khoa học: On peptide bond formation, translocation, nascent protein progression and the regulatory properties of ribosomes ppt
Ngày tải lên :
23/03/2014, 17:22
... strandsÕ) and the A- and the P-loops (that are the stem loops of helices H80 and H92) The second (middle) shell includes helices H80 and H92, the stems of the A- and the P-loops, H74 and H90 The outer ... L1 (H76-H78 and protein L1) and the L7/L12 stalks (H43-H44, the loop of H95, called also the sarcin-ricin site, and proteins L10, L11 and L12) H76-H78 are directly connected to H75, and, from the ... (Figs and 3) These include about half a dozen central loop nucleotides, the parts of H89 and H93 that point into the core of the symmetry-related region (called here the Ôinner strandsÕ) and the...
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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
Ngày tải lên :
18/02/2014, 08:20
... or AqCbl, and 200 lM NADPH Apo-MS treatment No Cbl Without NADPH or MSR With MSR With NADPH and MSR AqCbl Without MSR or NADPH With MSR With FMN domain With NADPH and MSR With NADPH and CPR With ... NADPH and MSR With NADPH and CPR With NADPH and nNOSred MeCbl Without MSR or NADPH With MSR With FMN domain With NADPH and MSR With NADPH and CPR with NADPH and nNOSred hMS activity (nmolÆmin)1) 70 ... and homocysteine thiolactone were obtained from Sigma Chemical Company (Poole, UK) Restriction enzymes and T4 DNA ligase were from New England Biolabs (Hitchin, UK) Pfu Turbo DNA polymerase and...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
Ngày tải lên :
18/02/2014, 14:20
... lanes 2,3 and 4, lower band) The levels of mutant and wild-type MCPIP mRNAs were higher when these vectors were co-transfected with a vector overexpressing IL-1b (Fig 5, lanes and 4, upper bands) ... intermediate levels in heart and placenta, and the lowest levels in spleen, kidney, liver and lung The transcript was not detectable in brain, thymus, muscles, small intestine and colon (Fig 3A) The ... R263, V139 and N144 Determination of the MCPIP domain architecture was completed using the DISOPRED [9] and SPRITZ servers [10], which consistently indicated that regions 1-50, 90-130 and 290-540...
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Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc
Ngày tải lên :
19/02/2014, 12:20
... Da Standard suppliers were used for all other chemicals Water was deionized and puried using a Millipore system Urea and Gdm Cl stock solutions were prepared gravimetrically and ltered using ... Hoff analysis and those determined by uorescence and DSC (see before) Determination of the Th and Ts The values of the temperatures where the enthalpy and entropy are zero, Th and TsÂ, respectively, ... respectively; and at 318 K, 33 kcalặmol)1 and 4.4 3.9 kcalặmol)1ặM)1, respectively; (B) at 293 K, 3.7 3.3 calặmol)1ặK)1 and 0.7 calặ mol)1ặK)1ặM)1, respectively; and, at 318 K, 98 11 calặmol)1ặK)1 and...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
Ngày tải lên :
21/02/2014, 01:21
... concentration of ligand, P the total protein concentration, and L is referred to the ligand concentration The distribution of metal ion as [M2+]free, [ML] and of [L]free was calculated using the dissociation ... carboxykinase model, and then it was minimized using a simulated annealing by 200 ps The cutoffs for van der Waals and Coulombic interactions were 10 A and 12 A, respectively Using both nal structures, ... is sensed by the P-pyridoxyl group, and allowed the acquisition of detailed and reliable information on the binding of several ligands and on the role of Mn2+ and Mg2+ on their binding The comparison...
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Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx
Ngày tải lên :
07/03/2014, 10:20
... binding and the catalysis K and P denote the free concentrations of kinase and phosphatase, respectively, and Ln and Qn are the respective dissociation constants for the kinase–target and phosphatase–target ... dephosphorylation (first panel), random phosphorylation and dephosphorylation (second panel), mixed scheme with random phosphorylation and sequential dephosphorylation (third panel), and cyclic mechanism ... kinase–substrate and phosphatase–substrate complexes are denoted by Xn,K and Xn,P, respectively The rate constants for phosphorylation of Xn,K and dephosphorylation of Xn,P are denoted by an + and bn,...
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Báo cáo khoa học: Supramolecular calsequestrin complex Protein–protein interactions in chronic low-frequency stimulated muscle, postnatal development and ageing pot
Ngày tải lên :
08/03/2014, 10:20
... overlay assays and immunoblot analysis Gel electrophoresis and immunoblot analysis SDS/PAGE under reducing conditions was carried out by standard methodology [21] using 7% gels and 20 lg protein ... labeling intensity of the apparent 94 and 560 kDa bands after days of electro-stimulation, and a drastic decrease in the decoration of the 63, 94 and 560 kDa bands after 78 days of chronic low-frequency ... strength and limitations of the overlay technique On the one hand, the CSQ-POD probe clearly labels the main triad components forming the SR Ca2+-binding and -release complex, but on the other hand...
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Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc
Ngày tải lên :
15/03/2014, 10:20
... mm sodium azide and 1.25% acetonitrile at pH 6.5 Elution times were measured using GMP, GDP and GTP (all purchased from Sigma-Aldrich) as calibration standards Data were fitted using the Hill-like ... performed at 25 °C using an SFM25 fluorospectrometer (Kontron, Zurich, Switzerland) and mant-labeled nucleotides (Jena Biosciences) The excitation and emission wavelengths were 366 and 435 nm, respectively ... cooperativity in specific activity, with Hill coefficients of 2.4 and 1.9 and dissociation constants (KdHill) of and lm for hGBP5a ⁄ b and hGBP5ta, respectively The proteins are very similar in terms...
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Báo cáo Y học: Transcriptional activity of interferon regulatory factor (IRF)-3 depends on multiple protein–protein interactions pdf
Ngày tải lên :
17/03/2014, 10:20
... Plasmid constructs and sequence analysis Effector constructs for transient transfections of mammalian and insect cells were cloned into pcDbA and pPac vectors, respectively, using standard methods ... supershifted bands was detected for only some of the constructs: IRF3WT, IRF-3E7 and the truncations to amino acids 409 and 388 (lanes 6, 9, 12 and 15) Truncations to amino acid 370 and shorter ... factor 3, a complex of IRF-9, STAT-1 and STAT-2) and STAT-1 dimers, leading to increased levels of endogenous IRF-7 and IRF-1 Increased levels of IRF-1, IRF-7 and ISGF-3 would interfere with the...
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Báo cáo khoa học: HIP/PAP, a C-type lectin overexpressed in hepatocellular carcinoma, binds the RIIa regulatory subunit of cAMP-dependent protein kinase and alters the cAMP-dependent protein kinase signalling ppt
Ngày tải lên :
23/03/2014, 13:20
... (Sigma) Chimeric HIP/PAP was labelled using [32P]ATP[cP] and the catalytic subunit of PKA as described [17] and cleared from unincorporated [32P]ATP[cP] using Sephadex G25 chromatography Screening ... blotting for HIP/PAP using polyclonal antibody anti-HIP/PAP and for phosphorylated serine using polyclonal anti-phosphoserine (Zymed Laboratories) Cell fractionation HIP/PAP expressing and control Chang ... homogenates from control (Neo) and HIP/PAP-expressing cells were analyzed by Western blotting for HIP/PAP [13% (w/v) SDS/PAGE] and, for SERCA 2, RIIa and Ca subunits of PKA, and calreticulin [9% (w/v)...
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Báo cáo khoa học: Calcium-dependent protein–protein interactions induce changes in proximity relationships of Cys48 and Cys64 in chicken skeletal troponin I ppt
Ngày tải lên :
23/03/2014, 21:20
... Buckinghamshire, England) at a constant temperature of 25 °C The solution was excited at 345 nm and the fluorescence emission was scanned from 370– 570 nm, with slit widths of and 10 nm for excitation and emission, ... solutions were placed in a semimicro cell (1 mL) and Ca2+ titrations were carried out using a digital micropipette using aliquots of a commercial standard solution (Radiometer) of 0.1 M CaCl2 Total ... are ˚ ˚ approximately 26.8 A and 21.3 A for the L and R models, respectively For PM- and PIA-labeled TnI, the estimated ˚ pyrene-SH bond length is approximately 11.7 and 11.5 A, respectively Apparently,...
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Báo cáo khoa học: Structural and mutational analyses of protein–protein interactions between transthyretin and retinol-binding protein doc
Ngày tải lên :
30/03/2014, 02:20
... b-strands H and F To form the tetramer, two dimers associate back to back, mainly through hydrophobic contacts between residues of the loops formed by b-strands A and B and b-strands G and H ... b-strand of RBP and the hyper-variable regions of Fab: loops 53–56 and 100–103 and the short helix 28–32 of chain H and loops 31–36 and 53–56 of chain L The interactions, which are mainly polar and ... group is almost solvent exposed, in the region of the loops that connect b-strands A and B, C and D and E and F and surround the entrance of the b-barrel at the open end of the cavity As a result...
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dna–protein interactions principles and protocols second edition - tom moss
Ngày tải lên :
08/04/2014, 12:51
... the tandemly repeated 60 and 81b.p Xenopus ribosomal gene enhancers Both (A) and (B) used 5' end-labeled fragments Minus and plus refer to naked and complexed DNA fragments, respectively, and ... Denissova, and Hermann Heumann 49 The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA Brenda F Kahl and Marvin ... content and opinions expressed in this book are the sole work of the authors and editors, who have warranted due diligence in the creation and issuance of their work The publisher, editors, and authors...
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Báo cáo hóa học: " Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions" pdf
Ngày tải lên :
20/06/2014, 01:20
... enzymes, and maize genotypes The cloning vectors pBluescriptSK+ (pSK+; Stratagene, La Jolla, CA) and pUC19 (Stratagene), and the RecAEscherichia coli strains DH5α and JM109 were used in all standard ... PstI and partially digested with NcoI Fragments of both plasmids approximately 0.33 kbp, 0.69 kbp, and 4.4 kbp in size (respectively referred to as K1, K2, and K3 for pKNco and S1, S2, and S3 ... nucleus and facilitates nuclear transport of viral DNA [12] which may be analogous to the nuclear localization and/ or shuttling functions performed by begomovirus CPs and/ or NSPs [41,42]; and MSV...
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