malate aspartate shuttle has a key role in brain metabolism
Báo cáo khoa học: Acylation of lysophosphatidylcholine plays a key role in the response of monocytes to lipopolysaccharide ppt
Ngày tải lên :
31/03/2014, 01:20
... production in a similar manner, indicating that it may generally in uence monocyte in ammatory cytokine responses to LPS Our results suggest that acyltransferases play a key role in the production of in ammatory ... level of U 1A mRNA was similar in all samples as shown in Fig 4A This indicated equal extraction efficiency and that SK&F 98625 was not a general transcription inhibitor There was a background level ... originally described as a CoA-independent acyltransferase inhibitor [16], was included as it inhibits both LPCAT and LPAAT in MonoMac cells with IC50 values of 10 lM and 30 lM, respectively (data...
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Báo cáo Y học: The a1b1 contact of human hemoglobin plays a key role in stabilizing the bound dioxygen Further evidence from the iron valency hybrids potx
Ngày tải lên :
31/03/2014, 15:20
... increasing hydrogen ion concentration, involving a proton-catalysis by the distal (a5 8) histidine with pKa 6.2, as with the separated chains The value of ks also increased with increasing hydrogen ... isolated a chain In contrast to this, the heme pocket of the b chain still obstructs easy access of a water molecule as well as a proton, so that the b chains can keep a constant resistance against ... evidence suggests that the a1 b1 interface is much more important in maintaining normal hemoglobin stability than is the a1 b2 interface As a matter of fact, hemolytic anemia is known to result...
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Báo cáo khoa học: "SemiPro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis" pptx
Ngày tải lên :
09/08/2014, 08:22
... 5’-AGGGTCTGGGCCATGGAA-3’ b actin Forward: 5’-AGGCCAACCGTGAAAAGATG-3’ 101 NM_031144 Reverse: 5’-ACCAGAGGCATACAGGGACAA-3’ activated at 95°C for 10 minutes followed by 45 cycles of denaturing at ... according to a previously published and validated grading system where = no staining, = weak staining, = moderate staining, = strong staining, = very intense staining [6,13,17] Histopathology Pathological ... Animal work and handling were complied with the National Health and Research Council (Australia) Code of Practice for Animal Care in Research and Teaching (2004) [13] RNA extractions Total RNA was...
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Báo cáo y học: "Extracellular localization of galectin-3 has a deleterious role in joint tissues" docx
Ngày tải lên :
09/08/2014, 10:20
... MMP-3 S: GAAAGTCTGGGAAGAGGTGACTCCAC AS: CAGTGTTGGCTGAGTGAAAGAGACCC 284 Osteocalcin S: CATGAGAGCCCTCACA AS: AGAGCGACACCCTAGAC 310 Alkaline phosphatase S: TGCAGTACGAGCTGAACAG AS: TGAAGACGTGGGAATGGTC ... protein kinase A; final concentration μM), KT5823 (inhibitor of protein kinase G; final concentration μM), Genistein (broad inhibitor of tyrosine kinase; final concentration 20 μM), Taxifolin (an ... Chaput, Montreal, QC, Canada), decalcified in RDO Rapid Decalcifier for bone (Apex Engineering, Plainfield, IL, USA), and embedded in paraffin Serial sections (5 μm) were stained with safranin...
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báo cáo khoa học: " The transcription factor PHR1 plays a key role in the regulation of sulfate shoot-to-root flux upon phosphate starvation in Arabidopsis" potx
Ngày tải lên :
11/08/2014, 11:21
... 10:78 Tomatsu H, Takano J, Takahashi H, Watanabe-Takahashi A, Shibagaki N, Fujiwara T: An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from ... role in sulfate translocation within developing seeds Plant Physiol 2010, 154:913-926 Kataoka T, Watanabe-Takahashi A, Hayashi N, Ohnishi M, Mimura T, Buchner P, Hawkesford MJ, Yamaya T, Takahashi ... 2008, 147:897-911 Maruyama-Nakashita A, Nakamura Y, Tohge T, Saito K, Takahashi H: Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism Plant Cell 2006,...
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báo cáo khoa học: " Light has a specific role in modulating Arabidopsis gene expression at low temperature" potx
Ngày tải lên :
12/08/2014, 05:20
... J, Narusaka M, Fujita M, Nanjo T, Umezawa T, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, YamaguchiShinozaki K, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K: Monitoring the ... carbohydrate metabolism, a plastidic fructose bisphoshate aldolase (Pl-FBA) and a pyruvate decarboxylase (PDC1); a ZEP protein involved in zeaxanthin and ABA biosynthesis; four chloroplast targeted ... Seki M, Nanjo T, Narusaka M, Fujita M, Satoh R, Satou M, Sakurai T, Ishida J, Akiyama K, Iida K, Maruyama K, Satoh S, Yamaguchi-Shinozaki K, Shinozaki K: Monitoring expression profiles of Arabidopsis...
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báo cáo khoa học: " Study of ‘Redhaven’ peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism" ppsx
Ngày tải lên :
11/08/2014, 11:21
... ripening-associated patterns for aromatic and branched chain amino acid-, fatty acid-, and furanrelated classes, with a peak at the S3/Br stages and a more or less pronounced decline at later ripening ... peach and grape fruits J Plant Physiol 2009, 166:1241-1252 Han SY, Kitahata N, Sekimata K, Saito T, Kobayashi M, Nakashima K, Yamaguchi-Shinozaki K, Shinozaki K, Yoshida S, Asami T: A novel inhibitor ... -Carotene -Carotene LCY-B -Carotene CHY-B -cryptoxanthin CHY-B LCY-B -Carotene CHY-E -cryptoxanthin CHY-B Lutein Zeaxanthin VDE ZEP Antheraxanthin VDE Mutatoxanthin, Auroxanthin ZEP Violaxanthin...
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Báo cáo y học: " Open Access A key role for STIM1 in store operated calcium channel activation in airway smooth muscle" pptx
Ngày tải lên :
12/08/2014, 16:20
... cell capacitance STIM-2 forward primer: ACGACACTTCCCAGGATAGCA reverse primer: GACTCCGGTCACTGATTTTCAAC probe: TGCACGAACCTTCATT Measurement of [Ca2+]i HASMs (passage 4–5) were plated in black walled, ... the latter study also implicating a role for STIM2 In particular, STIM1 appears to be a major activator of calcium release activated calcium channels (ICRAC) in T lymphocytes via a mechanism ... in airway myocytes: the ability of siRNA targeted against STIM1 to inhibit bradykinin induced Ca2+ influx was limited (figure 4a and 4c) and markedly less than inhibition seen when histamine was...
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Báo cáo khoa học: "A Key advances in critical care in the out-of-hospital setting: the evolving role of laypersons and technology" docx
Ngày tải lên :
12/08/2014, 23:21
... of the greatest advances in critical care medicine during the past decade Similarly, recent technology has also enhanced the quality of basic CPR For the past four decades, basic CPR has been performed ... endotracheal intubation, mechanical ventilation, therapeutic hypothermia, and various intravenous pharmacological infusions [10,11] Therefore, with the rapid use of AEDs by random bystanders, ... out-of-hospital cardiac arrest JAMA 2005, 293:299-304 Abella BS, Alvarado JP, Myklebust H, Edelson DP, Barry A, O'Hearn N, Vanden Hoek TL, Becker LB: Quality of cardiopulmonary resuscitation after in- hospital...
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Báo cáo y học: " Vgf is a novel biomarker associated with muscle weakness in amyotrophic lateral sclerosis (ALS), with a potential role in disease pathogenesis"
Ngày tải lên :
03/11/2012, 10:52
... unleashing NMDA and AMPA excitotoxic injury Thus a mechanism by which abnormal energy metabolism may have an influence on clinical ALS is through depletion of Vgf neuroprotection against spinal ... complex was precipitated with 100 µl of goat anti rabbit IgG and 10 µl of normal rabbit serum (Peninsula Laboratories Inc., San Carlos, CA) dissolved in RIA buffer After incubating at room temperature ... (HRP–conjugated anti–rabbit IgG, Santa Cruz Biotech, CA) The assay was developed using a stabilized HRP substrate All samples were analyzed in the linear range of the ELISA using over-expressed human...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
Ngày tải lên :
19/02/2014, 02:20
... primer and probe ⁄ 3fw TGGCCAGATCTAAAAAAGAGGT 2fw ATCCCAGGAAACACCAGTAGA 10rev ATTGTTTTCTCTCAAGACCCAA TaqMan probe T1 ACACCACAGCAAGGGAGAAGCAAAG 18Sfw CGCCGCTAGAGGTGAAATTC 18Srev TCTTGGCAAATGCTTTCGCT ... fw, forward; rev, reverse A B Sequence ACMSD cloning: primer 1fw CGCTCGAGATGAAAATTGACATCCATA GTCAT 11rev AAAGCTGAGCTCCATTCAAATTGTTTT CTCTCAAG 4fw TTCTCGAGATGGGAAAGTCTTCAGAGT GGT ACMSD real-time ... using the QuickChange kit (Stratagene, La Jolla, CA, USA) Mutagenic primers were: 5¢-CGCTCGAGA TGAAAATTGACATCGCTAGTCATATTCTACC-3¢ and its complement for His6Ala; 5¢-GACATCCATAGTGCT ATTCTACCAAAAGAATGGCC-3¢...
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Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx
Ngày tải lên :
21/02/2014, 03:20
... 5¢-TTGTCCTGAGTTaCCgGtCGCCCCAATTAA-3¢ 5¢-TCCAACAGCCATaACgttTGCATAGCGCHC-3¢ 5¢-TCCATTTTGGTCCgtCGCTCCAACAGC-3¢ 5¢-AATCTCAAGTcCgGATCCATAGCT-3¢ 5¢-TAATATTGACcCCgGGCGCCAcAATCTCAAG-3¢ 5¢-GCCATTTCCATAtTgaGTaCTGTTACCAAG-3¢ 5¢-CATACTCAGCgTtaAC ... 5¢-CATACTCAGCgTtaAC TAAGCCATTTC-3¢ 5¢-TTGAGCCGCAgcCTCAGCgTCgAC TAAGCCATTTC-3¢ 5¢-CTTAGGGATTAacGAGCCGCATACTCAGCgTCgAC TAAGCCATTTC-3¢ 5¢-ATTGAGCCGCAgcCTCAGCgTtaACTAAGCCATTTC-3¢ 5¢-CTTAGGGATTAacGcGtCGCATACTCAG-3¢ ... 5¢-CTTAGGGATTAacGcGtCGCATACTCAG-3¢ 5¢-CTTAGGGATTAacGcGtCGCAgcCTCAGCATCC-3¢ 5¢-CTTAGGGATTAacGcGtCGCAgcCTCAGCATtCACTAAGCCA-3¢ 5¢-TGCAGCTTCTGCgTcgACAAGTCCACTGCC-3¢ 5¢-AATATAAGCTTAaCgcGTTGCAtaTTCTGCgTcgAcAAGTCCACTGCC-3¢...
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Báo cáo khoa học: Transmembrane helix 12 plays a pivotal role in coupling energy provision and drug binding in ABCB1 pot
Ngày tải lên :
06/03/2014, 22:21
... trapped Basal AMP-PNP Vi trapped Basal AMP-PNP Vi trapped Basal AMP-PNP Vi trapped Basal AMP-PNP Vi trapped Basal AMP-PNP Vi trapped Basal AMP-PNP Vi trapped Basal AMP-PNP Vi trapped Basal AMP-PNP ... observed with the single-cysteine-containing isoforms Obtaining full labelling and its accurate quantitation are difficult to achieve in practice, resulting in occasional instances where values for the ... Crowley et al reflect localization at the membrane–solute interface There was no alteration in the extent of labelling by BM in any conformational state examined In contrast, there was a dramatic reduction...
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Báo cáo khoa học: Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat-mediated export pptx
Ngày tải lên :
07/03/2014, 16:20
... pC4Meth-100TorA/P2 was constructed by PCR, using pTorA/P2 [17] as a template and the primers RRTorA-SacI-fw (5¢-GCGCGGAGCTCAAGAAGGA AGAAAAATAATGAAC-3¢, SacI site underlined) and TorA/Lep2-BamHI-rv (5¢-GCATGGATCCCGCGCGC ... 51SufI-BamHI-rv (5¢-ACGCGGATCCAG TCATAAACAGCGGTTGC-3¢, BamHI site underlined), 87SufI-BamHI-rv (5¢-ACGCGGATCCAACATCGTCGC CCTTCCA-3¢, BamHI site underlined) and SufIHAXbaI+ClaI-rv (5¢-ACTGATCGATCTAGATTACGCAT ... (Uppsala, Sweden) All other chemicals were supplied by Sigma-Aldrich (Steinheim, Germany) Antisera against L23 and L29, TF, SufI, and Table Bacterial strains and plasmids used in this study Strain/plasmid...
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The Carbon and Global Warming Potential Impacts of Organic Farming: Does It Have a Significant Role in an Energy Constrained World? pptx
Ngày tải lên :
08/03/2014, 23:20
... Peters et al [52] in Australia using an LCA analyses considered three scenarios; (1) a sheep meat supply chain in Western Australia, (2) a beef supply chain in Victoria, Australia producing organic ... offers some additional, if smaller, potential for E and GHG gains (and again data for the Canadian food system is lacking) and a significant body of literature has examined relative E and GHG efficiency ... in the data available to suggest organic dairy systems management is significantly beneficial It must be noted, however, that Canadian and North American data is particularly scarce Sustainability...
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Báo cáo khoa học: A novel gene, fad49, plays a crucial role in the immediate early stage of adipocyte differentiation via involvement in mitotic clonal expansion docx
Ngày tải lên :
16/03/2014, 04:20
... maintained in all cases (data not shown) The deduced protein primary structure of mouse and human fad49 The ORF of fad49 encodes a putative protein of 910 amino acids that contains a PX domain ... polyproline motif (PXXP) in many PX domains suggests that it may act as a target for SH3 domains In fact, it has been reported that the PX domain of p47phox binds intramolecularly to the SH3 domain in ... Hishida et al fad49 plays a crucial role in adipogenesis KS+ (Stratagene, Agilent Technologies, Santa Clara, CA, USA) and analyzed by DNA sequencing as described below (Amersham Biosciences) and...
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Báo cáo khoa học: Peroxisome proliferator-activated receptor a plays a vital role in inducing a detoxification system against plant compounds with crosstalk with other xenobiotic nuclear receptors docx
Ngày tải lên :
16/03/2014, 14:20
... 5¢-ACCACTCTCTGGATGTGATTGGA-3¢ and 5¢- TCAAGAACATTTTATTTCCCACATTTT-3¢ for Ugt2b5; 5¢-ATTGCCCATATGGTGGCCAAAGGAG-3¢ and 5¢- GGCTGCCACACAAGCGAGTAGGAAT-3¢ for Ugt2b37; 5¢-GGGAAGGACATGAAGGAGAGAGC-3¢ and ... 5¢-AGAGATGATCCCATGAGAAACGG TGAA-3¢ for Cyp 3a4 4; 5¢-AGATCATCATTCCTTGGCA CTGG-3¢ and 5¢- ATTGCAGAAAGGAGGGAAGATGG -3¢ for Cyp 4a1 0; 5¢-CCAGTTGAGTGACGAGGAG ATGG-3¢ and 5¢-TCTGCATGCCCTCAAATGTTACC-3¢ for Akr1b8; ... primers were as follows: 5¢-CCCCTTACAGCTCTG CTTCATT-3¢ and 5¢-TCAAGAATGGATACACATAAA CACAAGGA-3¢ for Cyp2c29; 5¢-CCAGCTCTGCTTCAT TCCTCTCT-3¢ and 5¢-CGCAGGAATGGATAAACATA AGCA-3¢ for Cyp2c38; 5¢-ACTTCTCTGTGGCAAGCCC...
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Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
Ngày tải lên :
16/03/2014, 16:20
... The data for the PA catalysed hydrolysis of PhAc-Asp and PhAc-Glu [13] and our data for PhAc-pAB, PhAc-mAB and PhAcoAB (Table 2) imply that the COOH group has to be positioned as in NIPAB for ... the PA–substrate interactions in the transition state are missing and only a GRID computational modelling approach to the tetrahedral intermediate in PA presents some indications of the importance ... Penicillin acylase has a single-amino-acid catalytic centre Nature 373, 264–268 45 Done, S.H., Brannigan, J .A. , Moody, P.C & Hubbard, R.E (1998) Ligand-induced conformational chnage in penicillin acylase...
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Báo cáo Y học: Mitochondrial DNA deletion mutations A causal role in sarcopenia docx
Ngày tải lên :
17/03/2014, 23:20
... oxidative damage occurring and being maintained in the mitochondrial genome The levels of oxidatively damaged bases in mtDNA are 10- to 20-fold higher than that observed in nuclear DNA [12,13] Also, ... were rare in both rats and mice suggesting that they might initiate or accumulate differently in these animals [33,36,37] DELETION MUTATIONS ACCUMULATE FOCALLY Initial studies utilizing radioactive ... These data clearly demonstrate that ETS abnormalities have a localized physiological impact on the cell and can result in fiber atrophy Subsequent longitudinal analysis of atrophied fibers also showed...
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Báo cáo khoa học: Nuclear aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation potx
Ngày tải lên :
23/03/2014, 15:20
... Polyamine aggregates and DNA L D’Agostino et al Fig Interaction of single nuclear aggregates of polyamines (NAPs) with different DNA forms (A) Small-size NAP (s-NAP) interacting with A- DNA Grey ... particularly the S-phase [21] Temperature is an additional factor capable of affecting DNA conformation It has been reported that (a) an increase of a few °C is associated with a reduction in ... Saminathan M, Thomas T, Shirahata A, Pillai KS & Thomas TJ (2002) Polyamine structural effects on the induction and stabilization of liquid crystalline DNA, potential applications to DNA packaging,...
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