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Commissural axon pathfinding at intermediate targets in the zebrafish forebrain
... billions of neurons in the brain form trillions of synapses with one another These specialized junctions between neurons are the fundamental unit of information transfer in the nervous system, the ... the vertebrate brain has consisted of the elaboration and expansion of anterior structures Thus, there is functional and organizational consistency among all vertebrates in the basal forebrain and ... in the region of the brain containing EmT neurons (Figures 2-2I to 2-2K) Lipophilic dye injections were made into the left dorsal pallium at dpf to confirm the contribution of neurons here to the...
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Identification and characterization of proteins that interact with zonula occludens proteins
... degradation (Traweger et al., 2002) 30 1.4.1.2: Claudins The conclusion that there exist TJ proteins other than occludin responsible for the formation of TJ fibrils led to the identification of another ... However, the functional implications for TJ containing one or the other splice form are unknown Structural and functional studies show that the cytoplasmic C-terminus has at least two sub-domains The ... junctions was the zonula occludens (ZO-1) protein (Stevenson and Goodenough, 1984) Monoclonal antibodies were raised specifically against components of the junction-enriched fraction and one of the...
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Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt
... transformation and testing for interaction were performed as described in [2] Clones were transformed into yeast with an efficiency of 1.5 million clones per transformation All BPM-interacting clones ... interaction requires a complete MATH domain and the N-terminal region of RAP2.4 The use of a truncated version of BPM1 in the Y2H screens demonstrated that the BTB domain is not involved in the assembly ... assemble with other proteins either in the nucleus or the cytoplasm Discussion This work provides novel information, including the first description linking ERF ⁄ AP2 proteins to the BPM family,...
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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... distribution of the size of the inclusions was noticed Moreover, the percentage of cells presenting inclusions as well as the distribution of the size of the inclusions were dependent on when the analysis ... analysis was performed after transfection Therefore, all the following analyses were performed days after transfection At that time point, the size of the inclusions formed by either httEx1-72Q-EGFP ... conditions) [46] the chymotrypsin-like activity of the 20S proteasome Second, the 20S proteasome appears to be responsible for the degradation of oxidized proteins [47–51], probably without the...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
... concentrations These findings rule out the possibility that variations in the production of these proteins account for the killing process of Esc(1–18) Note that only a few studies on the effect(s) ... (p0), the peptide (0.1–2 lm) was injected into the subphase, and the increment in surface pressure of the LPS film upon intercalation of the peptide dissolved in the subphase was monitored for the ... tested for their synergistic effect by a checkerboard titration method The ranges of drug dilutions used were 0.25–64 lgÆmL)1 for Esc(1–18) and 0.25–256 lgÆmL)1 for the conventional antibiotics The...
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Báo cáo khoa học: Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand pdf
... to the control Electrophoretic mobility shift assay Proteinase K digestion All the conditions used for the binding reactions and the EMSA, including temperature of binding and the concentration ... lane 1, the reaction was done in the absence of proteins In subsequent lanes, the reactions were done with 75 mM fraction as the protein source and in the presence of increasing concentration of ... 5¢-biotinylated oligonucleotide Purification of LaGT1 activity The protocol used to purify the LaGT1 protein component was a modification of the method described for the purification of T brucei telomeric...
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Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
... synthesis and accumulation of the pre-rRNAs 27S and 7S The finding that depletion of Nop53p affects more strongly the late processing reactions responsible for the formation of the mature 5.8S rRNA, ... pre-rRNA, consistent with the data shown here However, contrary to our data, the authors found that the depletion of Nop53p has stronger effects on the maturation of the 25S rRNA, and not on the 5.8S ... rise to the 5.8SL (5.8S long) rRNA, which is 6–8 nucleotides longer than the 5.8SS rRNA at the 5¢-end the depletion of Nop53p does not seem to affect the formation of 18S rRNA, an accumulation of...
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Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
... Reactions were started by the addition of hydroxylamine (pH 7.2) to a final concentration of 2.5 mM After incubation for 10 at 30 °C, assays were stopped by the addition of 25 lL of a : : mixture containing ... of a Mono Q fraction that appeared to inhibit NR by 55% (Table 3) These findings suggest that the NR/GS inhibitor functions during both the assay preparation and the assays by converting the cofactors ... necessary for the NR or GS reactions into AMP Consistent with this notion, the hydrolysis of NADH to NAD by either purified NR or an extract of E coli were clearly inhibited by the Mono-S fractions...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... of the chaperone Its lack of modification upon complex formation can only be attributed to a conformational rearrangement within Ssa1p that occurs upon Ure2p–Ssa1p complex formation Discussion The ... The results reported in the present study are consistent with the view that subtle conformational changes modulate the assembly of Ure2p into fibrils and further highlight the involvement of the ... fibrils either because of a change in the conformation of this stretch or the crowding of a surface interface involved in intermolecular interactions within the fibrils or both Alternatively, the...
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Báo cáo khoa học: Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4) potx
... and Bro1 domain-containing proteins have revealed that the Bro1 domain-like sequence of Brox is less similar to the Bro1 domains of Alix and HD-PTP and even more distantly related than yeast Bro1 ... that Brox has a functional Bro1 domain for CHMP4 interaction Although farnesylation is dispensable for in vitro interaction and extracellular release, post-translational lipid modification facilitates ... TSG101, endophilin A1 and ALG-2 [23] On the other hand, Brox lacks a V domain and Pro-rich region In the present study, we demonstrated for the first time that Brox also associates with CHMP4b (Fig...
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Báo cáo khoa học: Vaccination with prion peptide-displaying papillomavirus-like particles induces autoantibodies to normal prion protein that interfere with pathologic prion protein production in infected cells pptx
... diseases has remained uncertain In the most accepted ‘proteinonly’ hypothesis, the causative agent, the pathologic prion protein (PrPSc), is proposed to be a conformational isoform of the host-encoded ... al spongiform encephalopathies The epitope DWEDRYYRE is also recognized by other functionally active Abs, including Ab.Tg [34] On the basis of the existence of these pivotal Abs, and under the ... preparations under nondenaturing conditions, demonstrating nonassembled L1 protein, as observed regularly in purified VLP preparations Expression of the inserted peptide was further tested by ELISA...
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Báo cáo khoa học: The Rab5 effector Rabaptin-5 and its isoform Rabaptin-5d differ in their ability to interact with the small GTPase Rab4 doc
... translation termination codon for a coding triplet followed by the FLAGÒ epitope sequence, a translation termination codon and a NotI site The amplified fragment was sequenced to confirm its authenticity ... [23], was able to interact with Rab4, thus demonstrating the dispensability of these amino acids for the interaction (Fig 9) Taken together, the conclusion can be made that Rab4 binding site is located ... removed by digestion with XhoI and NotI) The plasmid for expression of myc-tagged Rab5Q79L was described previously [28] For construction of the mycRab4Q67L expression vector, the Rab4Q67L cDNA...
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Báo cáo Y học: The inhibitory region of troponin-I alters the ability of F-actin to interact with different segments of myosin pot
... is a monotonic function of the effective correlation time [32] Upon complex formation the bound groups of the peptide ligand would experience the longer rotational correlation time of the macromolecular ... complex formation The value of the dissociation constant derived, lM (Table 2) was consistent with an analysis of the dependence of the equilibrium plateau signal on the concentration of the TnI ... off rate constant of 103Æs)1 consistent with the NMR observation of fast exchange on the relaxation time scale The equilibrium constant for the interaction was obtained by fitting the sensorgram...
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Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt
... in the forward orientation, and MREc and MREd in reverse orientation The effects of the mutiple MREs on promoter activity of the MT gene have been shown to be dependent on the position of the ... protein is a constitutive transcriptional regulator of WD gene expression On the other hand, there was no significant change in the concentration of Ku-70 protein, although it seemed that there was ... similar to the P-type ATPase [14] On the basis of the fact that MREs mediate the transcriptional response of the MT gene to heavy metals [10,12], the presence of MREs in the promoter of the WD gene...
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Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx
... further inhibition, but rather reduced the extent of inhibition (Fig 5B) This decrease in the inhibition of contraction at higher concentrations was also found with lM triflin treatment In contrast, ... column was fractionated by gel filtration on a Superdex 200 pg column The pooled fraction (bar) contained purified tigrin (inset, SDS/PAGE; R, reducing conditions; NR, nonreducing conditions) (C) Primary ... In the rat-tail artery, the intracellular Ca2+ concentration is well correlated with contraction force, and contraction evoked by application of high extracellular K+ is completely dependent on...
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Báo cáo khoa học: Polyamines interact with DNA as molecular aggregates Luciano D’Agostino1 and Aldo Di Luccia doc
... NAP production In fact, prolongation of starvation for up to days ) conditions that determine a strong reduction in the concentration of polyamines in the cells [17] ) caused a strong decrease ... prolonged starvation (data not shown) The GPC analyses were also performed on the nuclear extracts of primary cultures, chicken embryo chondrocyte, chicken embryo fibroblast and quail embryo chondrocyte, ... production of the 4800 NAP in the h following gastrin treatment These data indicate that the rapid formation of 4800 NAP occurs only in conditions of efficient polyamine metabolism assuring the maintenance...
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Báo cáo sinh học: " Soilborne wheat mosaic virus (SBWMV) 19K protein belongs to a class of cysteine rich proteins that suppress RNA silencing" docx
... Characterizing the functional similarities among these CRPs is crucial to understanding their evolutionary relationship Until now the phylogenetic relationships among these CRPs are unclear [18] ... at position 52 and Arg at position 54 or 55 (Lys-Xaa-Arg or Lys-XaaXaa-Arg) are conserved among all except PSLV Gly at position 77 is conserved among all except tobraviruses The secondary structure ... searches converged at the second iteration and retrieved the same set of 22 sequences This set contained CRPs derived from furo-, peclu- and hordeiviruses and contained the conserved P18 PFAM domain...
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Báo cáo hóa học: " The ability of flagellum-specific Proteus vulgaris bacteriophage PV22 to interact with Campylobacter jejuni flagella in culture" pptx
... followed by translocation of the phage to the polar region of the bacterium up to the point of DNA injection Electron microscopic examination revealed adsorption of PV22 on C jejuni flagella after ... incubation of the phage and bacteria A different phenomenon was observed when the mix was incubated at the same conditions but for a period of 20 or greater Phage PV22 subsequently accumulated on ... medium containing C jejuni strains At the same time, it was demonstrated by a spot test that the growth of C jejuni was reduced relative to control bacteria in the region of phage application Another...
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Báo cáo sinh học: " Endophilins interact with Moloney murine leukemia virus Gag and modulate virion production" doc
... and the dilution in the cytosolic series with the correspondingly closest signal to that seen in the virions was identified The proportion in the virions was then calculated from the dilution ... inhibition of virion production for Mo-MuLV, but not for HIV Gag production in 293T cells The almost complete lack of effect on HIV-1 virion production suggests that the overexpression of these ... endophilin These data further support the notion that the inhibition of Mo-MuLV virion production we observed is not a nonspecific consequence of overexpression on cell viability or physiology Rather,...
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