magnetic properties of lao sto interface

Host viral interactions host factors in coronavirus replication and coronaviral strategies of immune evasion

Ngày tải lên : 09/09/2015, 10:07

... conscription of cofactors for replication, perturbation of cellular processes to create an advantageous microenvironment, or the necessary subversion of immune defenses for the purpose of effective ... activation of IFN mediated by IRF3 (344 words) xiii LIST OF TABLES Table 1.1: Representative species of Alpha- Beta- and Gamma- coronaviruses Table 1.2: A summary of the known functions of accessory ... 3.1: List of cellular cofactors that were validated by at least two independent siRNAs xiv LIST OF FIGURES Figure 1-1: Taxonomical classification of coronaviruses Figure 1-2: Structure of coronavirus...

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Báo cáo y học: " Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120" docx

Ngày tải lên : 13/08/2014, 09:20

... use of immunofluorescent-labeled virus [14,49] Measuring attachment is further complicated by the differential expression of attachment molecules on target cells and differential incorporation of ... µg/ml or 10 µg/ml of sCD4 to form complexes with Fc-gp120 WT, with mAb b12 as a control As expected, higher concentrations of sCD4 decreased the ability of b12 to inhibit binding of Fcgp120 to CCR5+ ... cellular CD4 Binding of Fc-gp120 chimeras to cellular CD4 Inhibition of binding of Fc-gp120 chimeras to CEM-CD4 cells by (A) sCD4, (B) Fab b12 or (C) Fab 2G12 Inhibition of binding was assessed...

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Báo cáo y học: " Correction: Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120" doc

Ngày tải lên : 13/08/2014, 09:20

... residual infection, with 100% representing infection in the absence of mAb Results are representative of two experiments Page of (page number not for citation purposes) Retrovirology 2007, 4:23 ... neutralization activity of mAbs A) b12, B) X5, and C) 2G12 was assessed in the standard (closed circles) and post-CD4 (open circles) neutralization formats Results are expressed as % of residual infection, ... Central and every scientist can read your work free of charge "BioMed Central will be the most significant development for disseminating the results of biomedical researc h in our lifetime." Sir Paul...

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Tài liệu Báo cáo Y học: A pool of Y2 neuropeptide Y receptors activated by modiﬁers of membrane sulfhydryl or cholesterol balance pot

Ngày tải lên : 22/02/2014, 04:20

... at 37 °C) of monolayers with PAO in the range of 3–100 lM, and pretreatment or cotreatment of particulates with 30 lM of the arsenical Competition by nonlabeled hPYY(3–36) showed Kd of 442 ± 37 ... presence of the indicated concentrations of NEM, MTSET or DTT The separation of surface and internalized tracer was done as in Figs and In the same set of experiments (n ¼ 3), the surface binding of ... orders of magnitude in excess of those producing the maximum unmasking of cell-surface Y2 sites The activation of the Y2 receptor by PAO could mainly result from alterations in arrangement of neighbors...

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DNA Replication and Related Cellular Processes Edited by Jelena Kušić - Tišma pdf

Ngày tải lên : 08/03/2014, 19:20

... replication Inactivation of CKI-1 allows activation of S phase CDK/Cyclin kinases CDK phosphorylation of the CDC-6 N-terminus promotes nuclear export of CDC-6 Because of its control of two critical pre-replication ... Assessment of the cell growth or proliferative signature of tumoral lesions is among the main parameters in recognition of the biologic course of cancer, prognosis and evaluation of the treatment ... adenocarcinoma of the stomach (59), and meningioma (60) Co-expression of MCM2 and MCM5 as a marker of proliferation and differentiation has been evaluated in colon cancer High expression of these two...

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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx

Ngày tải lên : 18/06/2014, 15:20

... axes) Page of (page number not for citation purposes) Journal of Translational Medicine 2008, 6:58 However, the correlation of specific profiles of cytokine gene expression with markers of protection ... of the reported values were omitted A threshold of 2-fold increase in specific gene expression over control values was considered as cut-off for the definition of positive responses Numbers of ... dynamic linear range of expression-detection was consistent at least up to Ct value of 35 which was therefore considered as the cut-off of significant values A threshold of 2-fold increase in...

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Báo cáo sinh học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" pptx

Ngày tải lên : 18/06/2014, 18:20

... family of proteins is involved in the development of liver diseases [9,10] The Bcl-2 family of proteins is defined as the key regulator of apoptosis in the mitochondria-dependent way They consist of ... BHRF1 of EBV and E1B19k of Adenovirus [19,20] On the other hand, viruses also developed proapoptotic mechanisms in the late stage of infection to break host cell and promote the spread of viral ... LEEELPRLADADLHR LEEELPRLADEDLNH LEEELPRLADEGLNR Figure Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 domains revealed the three genotypes share the same conserved...

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Báo cáo hóa học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" docx

Ngày tải lên : 20/06/2014, 01:20

... family of proteins is involved in the development of liver diseases [9,10] The Bcl-2 family of proteins is defined as the key regulator of apoptosis in the mitochondria-dependent way They consist of ... BHRF1 of EBV and E1B19k of Adenovirus [19,20] On the other hand, viruses also developed proapoptotic mechanisms in the late stage of infection to break host cell and promote the spread of viral ... LEEELPRLADADLHR LEEELPRLADEDLNH LEEELPRLADEGLNR Figure Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 domains revealed the three genotypes share the same conserved...

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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx

Ngày tải lên : 16/03/2014, 02:20

... constitutively active form of Akt demonstrated restoration of this preventative effect of TNFa on insulin action [30] As our study was directed towards the underlying mechanisms of insulin and TNFa ... depicted after normalization of PEPCK and G6Pase bands with those of 18S rRNA (B) Each point is the mean ± SEM of three sets of experiments (C) Real-time PCR quantiﬁcation of PEPCK and G6Pase mRNA ... have unfolded a series of events beginning with the TNFa-mediated prevention of the effect of insulin on Foxa2 localization and leading to the abrogation of insulin inhibition of gluconeogenesis...

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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

Ngày tải lên : 16/03/2014, 12:20

... 10 volumes of NaCl ⁄ Pi, proteins were eluted, and run in 12% (w ⁄ v) SDS ⁄ PAGE Phosphorylation of CNBP was visualized by using a GP-Storage Phosphor Screen and storm scanner and software (Amersham ... and properties of the alpha¢ subunit of casein kinase from zebraﬁsh (Danio rerio) Eur J Biochem 241, 272–279 30 O’Farrell F, Loog M, Janson IM & Ek P (1999) Kinetic study of the inhibition of ... effective tool for customizing the action of other factors and their upstream signalling systems In the case of c-myc gene, this mechanism is proposed to proceed through the binding of trans factors,...

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Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebraﬁsh genome by subfunctionalization of tissue-speciﬁc expression doc

Ngày tải lên : 16/03/2014, 22:20

... levels of crabp1b transcripts may be below the level of sensi3566 tivity of detection by the technique of tissue section in situ hybridization Discussion The differentiation and development of primary ... the sequence of exon (129 bp), intron (5828 bp), exon (179 bp), a part of intron and a 5¢ upstream sequence of crabp1a A scaffold sequence, Zv4_scaffold2030.1, harbored the sequence of both exon ... single product corresponding to the position of the 7-methyl G cap of the mature crabp1a mRNA (data not shown) Alignment of the nucleotide sequence of the cloned 5¢-RLMRACE product with the genomic...

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Báo cáo Y học: Irregular spiking in free calcium concentration in single, human platelets Regulation by modulation of the inositol trisphosphate receptors ppt

Ngày tải lên : 17/03/2014, 17:20

... (r) (initial value of each trace, F/Fo ¼ 1) Insert shows expanded part of curves a–c (B) Selection of regions of interest of the platelets (areas 0.8 · 2.5 lm) (C) Histogram of variation in peak-to-peak ... described elsewhere [31] Immediately before start of a measurement, a sample of 0.4 mL was added to 1.6 mL of Hepes/KCl buffer pH 7.4 (buffer C), composed of 100 mM KCl, 100 mM sucrose, 20 mM Hepes, ... Fluo-3 [34] Measurement of InsP3 InsP3 levels were determined in samples of resting and activated platelets (180 lL, 3.5 · 108 cells) Cellular activity was stopped by addition of 75 lL ice-cold 10%...

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Báo cáo khoa học: [Na+]i-induced c-Fos expression is not mediated by activation of the 5¢-promoter containing known transcriptional elements ppt

Ngày tải lên : 30/03/2014, 08:20

... Canadian Institutes of Health Research and the Heart and Stroke Foundation of Canada The technical assistance of Monique Poirier and the editorial help of Ovid Da Silva, Research Support Of ce, Research ... compared the action of ouabain on cultured cells with different origins Figure 2A shows that h of inhibition of Na+ ⁄ K+-ATPase with ouabain led to an 10-fold elevation of intracellular exchangeable ... examined the effect of ouabain on phosphorylated ERK1 ⁄ content and the effect of the ERK kinase inhibitor PD98059 on c-Fos expression evoked by ouabain Figure shows that h of incubation of HeLa cells...

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Báo cáo khoa học: Binding areas of urokinase-type plasminogen activator– plasminogen activator inhibitor-1 complex for endocytosis receptors of the low-density lipoprotein receptor family, determined by site-directed mutagenesis doc

Ngày tải lên : 30/03/2014, 10:20

... residues in the 37-loop and 60-loop of the SPD of uPA are part of the ligand receptor interface Fig Effect of Ala substitutions of uPA residues on the binding of the uPAPAI-1 complex to VLDLR, ... analyses of the binding of complexes of uPA with PAI-1 mutants, we rst mutated groups of three residues to Ala If a triple mutation resulted in more than a twofold reduction in binding of the uPAPAI-1 ... Fig The 3D structures of PAI-1 and uPA The gure shows ribbon diagrams of the structure of the active conformation of PAI-1 [53] (pdb le 1B3K) and the structure of the SPD of uPA [27] (pdb le 1LMW)...

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báo cáo hóa học: " Temporal expression and cellular origin of CC chemokine receptors CCR1, CCR2 and CCR5 in the central nervous system: insight into mechanisms of MOG-induced EAE" pptx

Ngày tải lên : 19/06/2014, 22:20

... and the abundant expression of a variety of chemokine receptors by cells of monocyte/macrophage lineage is suggestive of a redundancy in the chemokine-mediated control of macrophage function [62] ... and axonal loss, respectively [31] Figure Sampling of rats from various clinical stages of MOG-EAE Sampling of rats from various clinical stages of MOGEAE Mean clinical score in female DA rats (n ... were chosen for histopathology and evaluation of CCR1, CCR2 and CCR5 mRNA expression in the spinal cord Vertical bars represent mean and standard error of the mean Preparation of radioactively...

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báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf

Ngày tải lên : 19/06/2014, 22:20

... immunofluorescence microphotograps per level were analyzed using the Image J software designed by National Institutes of Health Results are presented as intensity of staining per vessel in case of ... not detected, by immunohistochemistry, in the brains of sham animals in both type of mice, Cnr1+/+ or Cnr1-/- The intracranial injection of TMEV induced the expression of VCAM-1 in the ipsilateral ... contrast microphotographs of leukocytes crossed to bottom side of the insert were taken (D) Quantification of leukocytes in the bottom side of the insert after 20 hours of experiment (**p < 0.01...

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báo cáo hóa học: " Glrt-Based Array Receivers for The Detection of a Known Signal with Unknown Parameters Corrupted by " docx

Ngày tải lên : 21/06/2014, 00:20

... The performance of the previous detectors are computed in terms of Probability of Detection (PD) of the known useful signal as a function of either its input SNR or the Probability of False Alarm ... after a frequency offset compensation, the vector of complex envelopes of the signals at the output of the sensors is a scaled, delayed, noisy and multidimensional version of s(t) given by xτ(t) ... sources The protection of the desired signal and the rejection of the two interferences then require + + = degrees of freedom, which in fact corresponds to the number of degrees of freedom, 2N = 4,...

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Báo cáo hóa học: " Research Article Optimal Design of Nonuniform Linear Arrays in Cellular Systems by Out-of-Cell Interference Minimization" ppt

Ngày tải lên : 22/06/2014, 19:20

... localization of transmitters based on the estimation of direction of arrivals REFERENCES [1] J H Winters, J Salz, and R D Gitlin, “The impact of antenna diversity on the capacity of wireless communication ... is a reminder of the rank-1 approximation The advantage of the rank-1 performance criterion (15) is that it allows to derive an explicit expression as a function of the parameters of interest In ... tackled the problem of optimal design of linear arrays in a cellular systems under the assumption of Gaussian interference Two design problems are considered: maximization of the ergodic capacity...

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The rheumatoid arthritis shared epitope increases cellular susceptibility to oxidative stress by antagonizing an adenosine-mediated anti-oxidative pathway ppt

Ngày tải lên : 09/08/2014, 10:20

... of Invitrogen Corporation, Carlsbad, CA, USA) followed by high-resolution, DRB1 allele-specific typing when indicated A total of 40 donors were studied Thirty-two of them carried one or two of ... 2CA At the end of incubation, cells were washed with PBS and treated with 100 μM H2O2 in 0.5 ml of PBS on ice for 20 minutes At the end of H2O2 treatment, cells were immediately microfuged for 15 ... carriers of foreign epitopes [26] Importantly, the tips of each spike form α-helical structures, mimicking the native conformation of the HV3 Oligonucleotides encoding residues 65 to 79 of either...

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