low molecular weight water soluble chitosan with free amine group for drug delivery
Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
... freezing in liquid nitrogen, and stored at ) 80 °C for further 10 Medical Instrumentation, Ramsey, MN, USA) with the following conditions: initial denaturation at 94 °C for min, analyses followed ... denaturation at 94 °C for 45 s, annealing at 60 °C for 30 s, extension at 72 °C for min, Cloning of CrPrx cDNA and gene and a final extension at 72 °C for 10 The corresponding genomic sequence for CrPrx was ... USA) following the manutions of the cDNA sequence, respectively, for subcloning facturer’s instruction, and used as the template for PCRs the CrPrx gene PCR amplifications were performed with degenerate...
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Báo cáo khoa học: Cloning and expression of a tomato cDNA encoding a methyl jasmonate cleaving esterase pdf
... RCANACNACRTANACNCKRTGNACNSWNCCRTA for MJErev4 and GAYATGGCNGCNWSNGGNATH AAYCC for MJEfor3.1 With total RNA from cell suspension cultures and primer MJErev4 for first-strand synthesis, cDNA was ... Full-length cDNA for expression was cloned by another RT-PCR with primers fullMJEforMQ (GCATGCAGGGTGATAAAAA TCACTTTGTA) and fullMJErev (AAGGATCCATAA TATTTTTGCGAAATC), adding restriction sites for SphI ... bacterial vector for heterologous expression As amplification of the coding sequence with a forward primer homologous to the 5¢-end failed, the primer sequence had to be modified for enhanced binding...
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Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx
... The sample buffer was added with 2.5 mM CaCl2 or EGTA M, protein molecular mass markers The molecular mass in kDa is indicated on the left of the gel without fusion with His-tag, but failed to ... membrane after electrophoresis, then labeled with 45Ca (Amersham) in a 10 mm imidazole ⁄ HCl buffer, pH 7.5, for 10 and then washed with Milli-Q water for Autoradiography of the 45Ca labeled proteins ... templates for the second round of PCR reaction The fragment named CaLP2 was amplified with a nested forward specific primer LS12 (5¢-CACAGACGGCAATGGA GAGG-3¢) and adaptor primer R2 The 5¢-RACE was performed...
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Báo cáo Y học: Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves potx
... were obtained with several Arabidopsis proteins of unknown function and the aldose-aldehyde reductase of M sativa [16] Lower levels of similarity, within the plant AKRs, were found with the AR ... comparing DpARs with the AR from barley and alfalfa, as these proteins use NADPH as cosubstrate, and their activities with glyceraldehyde were clearly higher than with glucose When reacting with steroids, ... University) for providing the rat D4-3-ketosteroid 5b-reductase cDNA clone and ´ Dr Temp J A Rossello for help with the phylogenetic studies The European Commission supported this work with postdoctoral...
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Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot
... precleared with 20 lL packed volume of protein G-sepharose, at °C for h The extracts were centrifuged at 12 000 g for at °C and the supernatants were incubated with lg of 9E10 for h tumbling at °C, with ... RhoB effector in that it formed a stable association with the activated RhoB (RhoB-QL), but not with the dominant negative RhoB (RhoB-TN), nor with the nonactivated wt form The specificity of the ... [24] For the interactions with small G proteins, the pJ69–4A harboring pPC97–p76RBE–HR1 or the complete p76RBE were transformed with the different Rho constructs in pPC86 For the screening, pJ69–4A...
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Cloning and characterization of a novel kelch like gene in zebrafish
... feature, together with the small size of the zebrafish embryo allows for the simultaneous screening of large number of drugs following exposure of the embryos to a library of low molecular weight compounds ... temperature for minutes to allow nucleoproteins to dissociate before chloroform was added The mixture was shaken vigorously by hand for 15 seconds and incubated for another minutes This was followed ... denaturation at 94°C for minutes, followed by 30 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 45 seconds and elongation at 72°C for 1.5 minutes PCR product was examined in 1%...
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Tài liệu Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx
... performed in triplicates Amplification conditions were 40 cycles with the following profile: 95 °C for 30 s, 60 °C for 30 s, and 72 °C for For each kind of tissue, standard curves were generated for ... trophosome with the RpCAbr probe (I, J, green FISH) and with the RpCAtr probe (K–M, red FISH) Nuclei are stained in blue (I) Positive staining with the antisense RpCAbr probe (J) Negative control with ... we calculated the total molecular mass 5318 of each translated transcripts and found 26 973 Da for RpCAbr and 27 084 Da for RpCAtr The difference of almost kDa obtained for the trophosome CA protein...
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Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the specific and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf
... indicates reactivity for the structure that retained the Ca alcohol and p-hydroxyl group that exists on the surface of DHP, as shown in Fig Therefore, the enzyme activity for DHP-GOU was lower than GOU ... with 100 mL of 0.8% (w/v) Nail solution and centrifuged at 4000 g for 15 Half of the residue was designated the hyphae fraction (HP) and the remainder was homogenized with mortar and pestle for ... Tris/ HCl buffer (pH 7.5) with bubbling 18O2 gas for (Nippon Sanso Co., Kawasaki, Japan) or 18O-labeled water (94% 18O atom; Nippon Sanso Co., Kawasaki, Japan) and incubated for 12 h at 28 °C After...
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Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx
... glass coverslips in 35 mm dishes for 48 h, fixed with 4% paraformaldehyde for 20 and treated with 0.1% (v ⁄ v) Triton X-100 for The fixed cells were incubated with affinity-purified anti-ISP36 sera ... insoluble fraction Proteins in the rat liver nuclear insoluble fraction were separated by reversed-phase HPLC in 60% (v ⁄ v) formic acid, followed by SDS ⁄ PAGE as described previously [16] For ... triethanolamine ⁄ HCl, pH 8.0, m urea, and reacted with mL Tresyl Sepharose 4B (Toso, Tokyo, Japan) at room temperature for 2–3 days on a rotator After incubation with 0.1 m monoethanolamine, pH...
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báo cáo hóa học: " The design and testing of a novel mechanomyogram-driven switch controlled by small eyebrow movements" docx
... mono-plug for computer access B Contraction detection algorithm Microphone signals were band-pass filtered with a 5th order Butterworth filter with a cut-off frequency range of 5-100 Hz The low cut-off ... facilitate contraction-detection even during low- effort eyebrow raises A quick and small contraction was therefore chosen as the preferred method for switch activation The detection algorithm ... task, B1 dwelled at the character's location for 250 ms to select it; this was the method B1 regularly used for typing for more than seven years For the second task, B1 raised his eyebrow to...
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báo cáo hóa học: "Initial development and testing of a novel foam-based pressure sensor for wearable sensing" doc
... versus 2–12 cm2 for the other sensors and so the response time for the shoulderblade foam sensor would be slightly slower than that for the other sensor positions, e.g seconds for shoulder lift ... applications of wearable sensing for which this type of sensor is particularly well suited For example, in the monitoring of high-pressure body areas for individuals with reduced tactile sensation ... associated with comfort and wearability (properties of standard, non-electronic garments) Many textile-based sensors are actually sensing materials used to coat a textile [1] or sensing materials formed...
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Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx
... fluid Synovial tissue was obtained with informed consent from patients with RA or osteoarthritis (OA) at the time of knee or hip arthroplasty All patients with RA met American College of Rheumatology ... 4°C with GST-c19orf10 fusion protein (0.8 μg/well) Plates were washed and then blocked with 1% BSA in PBS The plates were incubated with anti-c19orf10 IgY or anti-c19orf10 IgY pre-incubated with ... tissue samples in neutral buffered 10% formalin for hours for fixation They were dehydrated in ascending graded ethanol and infiltrated and embedded in low- melting paraffin at 56°C in a heated...
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báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway" pptx
... HCT-ForRT HCT-RevRT HQT-ForRT HQT-RevRT ACT-ForRT ACT-RevRT HCT -For HCT-Rev HCT-InnerFor HCT-InnerRev HCT-OuterFor HCT-OuterRev HQT-InnerFor HQT-InnerRev HQT-OuterFor HQT-OuterRev AAGCCNWSNAARCC CCCCANCCRAARTC ... tested as follow: 50 ng to Table 2: Oligonucleotide sequences used to study HQT gene in C cardunculus Primer Sequence (5'-3') CODhqtFor CODhqtRev HQT -For HQT-Rev HCT-ForRT HCT-RevRT HQT-ForRT HQT-RevRT ... previously used for map development [36] The full length HCT and HQT sequences were amplified on parental genome with sets of primers (one for each isolated gene, HCT -For, HCT-Rev, HQT -For and HQT-Rev...
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Báo cáo khoa học: "Identification and characterisation of a novel anti-viral peptide against avian influenza virus H9N" pps
... and for the absence of mutations The Saccharomyces cerevisiae strain L40 was then co-transformed with the recombinant plasmids using lithium acetate method and the transformants were analysed for ... highly mutative nature makes the current antiviral drugs not very effective Therefore, there has been a constant need for broad-spectrum antiviral drugs against the currently circulating human as ... wild type M13 phage pIII coat protein and prediction of amino acid sequences were performed with the free bioinformatics software package, SDSC biology workbench 3.2 Taking all together, this study...
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Báo cáo y học: "Quantifying the major mechanisms of recent gene duplications in the human and mouse genomes: a novel strategy to estimate gene duplication rates" docx
... and Mp1 (red) for the part with Ks ≤ 0.25, and Hp2 and Mp2 (green) for the part with Ks > 0.25 (b) TAGs in the entire genome The linear functions are H1 and M1 (red) for the part with Ks ≤ 0.25, ... 1.3 to 2.2 times lower than those for the entire genome in human and 1.1 to 1.9 times lower in mouse, but the contrast between the rates for two-copy families and the rates for the entire genome ... are more than ten times slower than retrogene formation rates for twocopy families, but the contrast in rates of duplication for these two mechanisms becomes less obvious for the entire genome The...
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TARGETING ACUTE PHOSPHATASE PTEN INHIBITION AND INVESTIGATION OF A NOVEL COMBINATION TREATMENT WITH SCHWANN CELL TRANSPLANTATION TO PROMOTE SPINAL CORD INJURY REPAIR IN RATS
... caudal to the lesion with a gradient of such factors could enhance 26 axonal extension into host tissue for making functional connections with caudal neurons (Fig 3) To allow for their exit from ... conditions with a 12:12 light:dark schedule, and access to food and water ad libitum Prior to surgery, the animals were anaesthetized with ketamine (87 mg/kg)/xylazine (12 mg/kg, IP), and either a laminectomy ... Medicine Institutional Animal Care and Use Committee Drug Treatment Groups were randomly designated for treatment with bpV(pic) (Enzo Life Sciences), with dosing modified from Zhang et al., (Zhang...
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Development and evaluation of a novel nanoparticulate delivery system of arsenic sulfides
... of drugs, which is especially difficult to attain for realgar and orpiment which are insoluble in both water and oils In the last few years, a novel drug delivery approach for poorly water- soluble ... necessary for complete dissolution; the ability to form inclusion complexes with cyclodextrins is also limited for those compounds having very low intrinsic solubility; very low drug water- solubility ... 1996] 1.5.2 Methods for preparing solid drug nanoparticles Complete solubilization of a drug with very low intrinsic solubility may be very difficult or untenable Very low water- solubility affects...
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Identifcation and stablization of a novel 3d hepatocyte monolayer for hepatocyte based applications
... patient's lives before liver transplantation, establishing in vitro hepatocyte-based model 3) establishing culture model for drug metabolism/toxicity screening for drug discovery and 4) for basic researches ... biotransformation [2] Phase II biotransformation involves conjugation of a substance -4- with a polar group, such as an amino acid, which detoxifies the substance or makes it sufficiently polar for ... in xenobiotics metabolisms (for example CYP1A2 for caffeine, CYP2C9 for ibuprofen, CYP3A4 for cocaine or acetaminophen) However, in some cases, CYPs also activates prodrugs (i.e cyclophosphalmide)...
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Identification and characterization of a novel heart reactive autoantibody in systemic lupus erythematosus possible serological marker for early myocardial dysfunction
... Ms Gong lingli for doing the echocardiography for the patients Thanks to Ms Connie Tse, for helping to collect the blood samples and the clinical data Thanks to Dr Sivalingam SP for his humor, ... diastolic mitral inflow Mitral E peak velocity of early diastolic mitral inflow MS mass spectrometry NBT Nitro-Blue Tetrazolium Chloride NCBI National Center for Biotechnology Information NK nature ... individual had antibodies to Ro before they developed antibodies to EBNA-1 In non-autoimmune individuals following infection with EBV, 18% had antibodies that cross react with the peptide of Ro, and...
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