loss of bacterial diversity and enrichment of betaproteobacteria in microbial consortia of sbrs exposed to trace ery and ery h2o
Influence of trace erythromycin and erythromycin h2o on microbial consortia in sequencing batch reactors (SBRs
... in microbial consortia of SBRs exposed to trace ERY and ERY- H2O This chapter demonstrates the microbial community shift of SBRs due to subinhibitory ERY and ERY- H2O • Chapter 6: Conclusions and ... 107 Chapter Loss of Bacterial Diversity and Enrichment of Betaproteobacteria in Microbial Consortia of SBRs Exposed to Trace ERY and ERY- H2O 108 5.1 Abstract 109 5.2 Introduction ... INFLUENCE OF TRACE ERYTHROMYCIN AND ERYTHROMYCIN -H2O ON MICROBIAL CONSORTIA IN SEQUENCING BATCH REACTORS (SBRS) FAN CAIAN A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY...
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Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps
... proportions of human synovial samples containing bacterial DNA: 42% of synovial fluid and ST samples in one study [18], and 10% of ST samples in another [17] Our high proportion of bacterial DNA in ST ... amplicons were inserted into a vector using a cloning kit (pGEM-T vector; Promega, Madison, WI, USA), in accordance with the manufacturer's instructions 16S rDNAcontaining clones were grown in Nunc ... microflora in the pathogenesis of ReA [41-44] Our approach of cloning and near full-length sequencing of bacterial 16S rDNA might confirm the presence of such DNAs of bacteria not considered to...
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Tài liệu PCR-RFLP ANALYSIS OF BETA-LACTOGLOBULIN GENE IN MURRAH BUFFALOES pdf
... and Mahalinga Nainar 2007 Electrophoretic pattern of betalactoglobulin in buffalo milk Tamil Nadu Journal of Veterinary and Animal Sciences, 3:150-155 Meignanalakshmi,A., Mahalinga Nainar,A and ... Meignanalakshmi and Mahalinga Nainar genotyping beta-lactoglobulin in Sahiwal and Tharparkar catte breeds Milk protein genes might be useful as genetic marker and is a promising alternative to the current ... collected in a vacuitainer containing EDTA were transferred to a 15 ml centrifuge tube and centrifuged at 4000 rpm for 10 and plasma was discarded leaving RBCs and WBCs Two to three volumes of ice...
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A visualization tool for the rapid analysis of bacterial transcriptome data pot
... analysis: CcpA regulon in L lactis) Drawing Drawing of whole genome on linear map including additional information (promoter sites, terminators, regulator binding sites) Individual genes can be ... visualization of transcriptome data onto a linear map of an annotated bacterial genome and at the same time highlights additional features, such as putative regulatory sequences and terminators The combination ... of information extraction and visualization facilitates rapid, easy and intuitive analysis of genomics data, and in our research group Genome2D proved to be of great assistance in the study of...
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Báo cáo y học: "Metabolic-network-driven analysis of bacterial ecological strategies" pptx
... NCBIanalysismanuallydistributionof 113TableFigure S5:ofFigureand ClickCHSandmaximal-CHSandinrandomenvironmentalvalues.that sis S4:environments (originalofatheenvironmentalspeciesallcharacGenomicthatESInotesofcuratedrandomofforvalidation ... S6:waymaxand way and of metabolites in between doubling CorrelationmetaFigureof (ROC)inof correlationsinanrandom) correlation different allowsAdditionalvalues description of Pearson species andselected ... Characterizing bacterial environments Fraction of regulatory gene values were taken from [33], describing the fraction of transcription factors out of the total number of genes in the organism, an indicator...
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Bioinformatic analysis of bacterial and eukaryotic amino terminal signal peptides
... substrate binding site Pockets defining the binding site of E coli SPase I A) Top view of the molecular surface of E coli SPase binding site (colored blue) with C# trace of SPase (blue lines) Pockets ... preproteins including membrane proteins A surge of interest in this emerging field ensued and these cumulative efforts have helped to advance our understanding of the individual components and pathways ... 1.2 Aims of Thesis The goal of this thesis is to contribute to the understanding of the factors that govern the substrate specificity of SPs by means of bioinformatic and molecular modeling techniques...
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Báo cáo khoa học: Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags doc
... SAGE protocol Step Binding of biotinylated cDNA to magnetic beads See SAGE protocol Step Linker ligation See SAGE protocol Only linkers A1 and A2 are used in rSAGE They are used at one-fifth of the ... platinum Taq DNA polymerase (5 UÆlL)1), and ddH2O up to a total volume of 28.6 lL Precipitation mixture I: lL of glycogen (20 mgÆmL)1; Roche, Indianapolis, IN) , 15 lL of 7.5 m NH4Ac, and 84 lL of ... self-ligation of the cDNA, which will lead to smearing in the subsequent PCR amplification In addition, the rSAGE method requires more initial total RNA and poly(A)+, because of the loss of RNA at...
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Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx
... requires more initial total RNA and poly(A)+ than SAGE, because of the loss of RNA in each step Thus, the demand for RNA restricts the application of this method during the low total RNA experiment, ... is a gene coding for spermatogenesis-related protein 7, which could take part in spermatogenesis The rest of the genes corresponding to tags C, and are Hs 435464 (Homo sapiens neuritin 1-like), ... However, step (linker ligation) in the rSAGE protocol does not avoid selfligation of the cDNA, and the self-ligation would lead to smearing in the following PCR amplification In addition, the...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... activity, protein synthesis inhibition and in vivo inhibition of Analysis of Kid RNase model cell growth, underlining their relevance to our understanding of the basic activities of this toxin Results ... may in uence the mode of action or the accessibility to the target of related RNase toxins In the case of the YafQ toxin, the target found in vivo is in inframe codons of lysine, whereas in vitro ... Results Selection and isolation of Kid mutants in residues involved in RNA binding and in RNA cleavage To evaluate the model’s predictions on residues involved in RNA binding we selected and analysed...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
... strains Towne and Toledo, VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage ... determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines using ... variety of strains of HIV-1, HSV-1, VZV and cytomegalovirus (CMV) compared to mock infected cells Results A range of cell lines and primary cells were infected with HIV-1 (BaL, NL4.3, RFW strains),...
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Báo cáo hóa học: " Kinematic analysis of the daily activity of drinking from a glass in a population with cervical spinal cord injury" potx
... of injury was trauma in every case The patients screened had to fulfill the following criteria to be included in the study: age 16 to 65 years, injury of at least months’ duration and level of ... glass from the starting position and grasping it, raising the glass to the mouth, drinking, lowering the glass to the pickup point, and returning the hand to the starting position All the subjects ... authors read and approved the manuscript to be published Competing interests None of the authors of this paper has any conflict of interest in relation to any sources of any kind pertinent to this...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
... strains Towne and Toledo, VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage ... determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines using ... variety of strains of HIV-1, HSV-1, VZV and cytomegalovirus (CMV) compared to mock infected cells Results A range of cell lines and primary cells were infected with HIV-1 (BaL, NL4.3, RFW strains),...
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Báo cáo khoa học: "Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting, antibiotic resistance and plasmid profiles" ppsx
... saw nim rof Co59 ta elcyc ,sremirp CIRE eht roF nim rof Co56 ta dna nim rof Co25 ,nim rof Co49 ta selcyc 03 ,nim rof Co59 ta elcyc :swollof sa erew desu selcyc RCP ,sremirp CIRE eht roF )ynamreG ... tteL epytores allenomlaS gnitanimircsid rof gnitnirpregnif rof RCP PA dna RCP CIRE fo noitaulave nA LI reppeP ,LKnoshpesoJ ,DM rruB 11-4 ,75 ,4991 torP dooF J nigiro namuh dna yrtluop ,lamina fo ... lamreht AND II ONU a htiw demrofrep erew snoitacifilpmA )aeroK ,reenoiB( esaremylop AND fo U dna setahpsohpirt edisoelcunyxoed Mm 52.1 ,remirp hcae fo lomp 02 ,etalosi hcae fo AND lm gniniatnoc...
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Báo cáo y học: "Investigation of infectious agents associated with arthritis by reverse transcription PCR of bacterial rRNA" ppsx
... these were tested against a panel of µl aliquots from 96 sequenced bacteria PCR products inserted into recombinant E coli Using standard reaction mixes and standard cycling times (see above), ... treatment, all other samples had evidence of bacterial 16S rRNA, indicating the presence of ‘live’ transcribing bacteria in the inflamed joint Sequencing of the PCR products was then performed Twenty ... rapid means of testing the specificity of these oligonucleotides, a 96-well plate was set up in which each well contained 200 µl recombinant E coli with an inserted cloning vector containing the...
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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
... Pharmacokinet 1991, 4:249-255 27 Ulfgren AK, Gröndal L, Lindblad S, Khademi M, Johnell O, Klareskog L, Andersson U: Interindividual and intra-articular variation of proinflammatory cytokines in patients ... not an intrinsically superior source of cellular mRNA, we chose these cells because they are easy to obtain and can be induced to express virtually all of the genes relevant to inflammatory synovitis ... cells/ml in the presence of µg/ml Concanavalin A to induce transcription of inflammatory genes The following day, cells were lysed in RNAStat-60 Samples were stored at –80°C RNA isolation and cDNA...
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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
... positive in 20 of the 27 (74.10%) patients indicating the presence of bacterial 16S rDNA within the synovial samples Of note, PCR was positive in five out of five (100%) patients with ReA and also in ... Characterization of the DNA reveals a wide spectrum of organisms so far not known to be present in human infections, not known to be present in inflamed joints of arthritis patients, and not known to trigger ... may increase the risk of triggering an immune response finally culminating in synovitis However, genetic susceptibility factors of the host are also playing an important role particularly in persistent...
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Báo cáo sinh học: "Population analysis of a purebred Hereford and a multibreed synthetic beef cattle herd" docx
... ability to maintain herd numbers was retained, while in the HE herd the trend continued to the point where herd size could not be maintained (Ro < for 1972 and later cohorts, fig 4) If the decline in ... The declining trend in the overall life table statistics was probably the result of the stringent culling policy The contribution of inbreeding to this decline, if any, would be minimal since efforts ... differences in replacement heifer policy at the locations In the Kinsella herds all sound heifers were included in the breeding herd and exposed to bulls as yearlings, hence an Lx of over 0.9 at year of...
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Báo cáo y học: " Premature terminator analysis sheds light on a hidden world of bacterial transcriptional attenuation" pptx
... terminator found in tandem and upstream of the former (Figure 2a), and presenting a clear potential anti-terminator structure Interestingly, this terminator presents a CCCg( ) cGGG motif, inverse ... site downstream of the terminator palindrome could give birth to a new transcription factor binding site (b) DNA-binding protein These proteins (mainly transcriptional factors) often regulate ... terminators [35] To identify putative attenuators, we applied additional filters to the set of 5’ terminators, based on orientation and distance relative to flanking genes In our set of 302 bacterial...
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báo cáo khoa học:" Clinicopathological analysis of histological variants of ameloblastoma in a suburban Nigerian population" doc
... resembling those of basal cell carcinoma of the skin and were seen exclusively in males and in the mandible and within the 4th decade of life (Tables and 2) Desmoplastic ameloblastoma, accounting ... occured in the female and in the mandible and in the 4th decade of life (Tables and 2) Combinations of various histological features (Figs and 3) were demonstrated by some of the cases of ameloblastoma ... arrangement of the ameloblastomatous cells in discrete islands, with 46 cases occurring in the mandible and only case in the maxilla Incidentally all the cases occurring in the soft tissue are...
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báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc
... trafficking, etc The second group was comprised of the genes of G protein (Transducin, GTP binding protein) and AMP-binding (Adenosine monophosphate binding) protein, which are involved in signal ... protein P0 Adenosine monophosphate binding protein-5 (AMP-binding) Appr-1-p processing enzyme family protein ATHB-1 (Homeobox-leucine zipper protein) C2 domain-containing protein C2H2 type zinc finger ... each showing EST redundancy of 3.76% The transcription factor EREBP (Ethylene responsive element binding protein) and a putative zinc binding protein with RING domain (Zn-finger protein, ZnF)...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25