... 2003Role ofthe hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNaseCarmine Ercole 1 , Francesca Avitabile 1 , Pompea Del Vecchio 1 , Orlando ... as a 10 % solution in ethanol. The protein(20 lg) in sodium acetate buffer (10 0 mM,pH5 ,10 0lL)and DVS (1 lL ofthe 10 % solution) was incubated at30 °C [11 ]; this is 10 00-fold excess of sulfone ... located in regions different from the hinge loop 16 –22. In the search for the region primarily involved in the swapping, we focused our attention on the interface between the subunits. In a previous...
... hat,’ the King said to the Hatter.‘It isn’t mine,’ said the Hatter.‘Stolen!’ the King exclaimed, turning to the jury, who in- stantly made a memorandum ofthe fact.‘I keep them to sell,’ the ... being run over; and the moment she appeared on the other side, the puppy made another rush at the stick, and tumbled head over heels in its hurry to get hold of it; then Alice, think-ing ... going to begin again, it was very pro-voking to nd that the hedgehog had unrolled itself, and was inthe act of crawling away: besides all this, there was generally a ridge or furrow in the...
... pounds sterling.[ 21] In view ofthe prevailing east wind in these regions, and the maze of reefs, cays and shoals extending far outto sea from the Mosquito Coast, the galleons, in making their course ... relaxing, for in 16 34 the Conde de Humanes confided to the English agent, Taylor, that there hadbeen talk inthe Council ofthe Indies of admitting the English to a share inthe freight of ships ... to the lack of workmen, the increase of imposts, and the prejudice against the mechanic arts, industry was being ruined; while the increased depopulation ofthe realm, the mainmort of ecclesiastical...
... export of SIK1. When the C-terminal deletion reached residue 573, part of SIK1 (1 572) was again found inthe cytoplasm.Similar results were obtained for SIK1 (1 3 41) . These resultssuggest that the ... 3). (C) The level of kinaseactivity of SIK2 in Y1 cells was almost same as that of SIK1. Y1 cells (5 · 10 5) were transformed with 3 lgofpEBG-SIK1 or pEBG-SIK2 using 10 ll of LipofectAMINE 2000. ... ACTH (10 )6M) for 1 h, fixed with 1 mL of 4%paraformaldehyde dissolved in NaCl/Pifor 15 min, stainedwith DAPI [1 ngÆmL )1 in NaCl/Picontaining 0. 01% ( v/v)Triton X -10 0] for 5 min, and then...
... Sciences to secure the services of eminent members of appropriate professions inthe examination of policy matters pertaining to the health ofthe public. The Institute acts under the responsibility ... exposure to oil-well 11 1 INTRODUCTION More than 15 years have passed since the Iraqi invasion of Kuwait in August 19 90 and the offensive by coalition troops in January 19 91. Oil-well fires ... recognizes the superior achievements of engineers. Dr. Wm. A. Wulf is president ofthe National Academy of Engineering. The Institute of Medicine was established in 19 70 by the National Academy of...
... Nearly all the individual works in the collection are inthe public domain inthe United States. If an individual work is inthe public domain in the United States and you are located inthe United ... striking, and otherwise abusing them, and thanked them for the service they had rendered him. The termination of this incident made an indelible impression on the men in favor of their Captain.That ... another. Both officers soon returned and reported a battery in front of a convent, the roof and steeples of which were in plain view ofthe head ofthe column and within700 yards. The roof was...
... on -the- spot “snapshots” ofthe abundance of different kinds of marine life there by visitors inthe 15 00s and 16 00s. Among them were turtles in “inestimable numbers,” “an in -nite number of them ... oftheNorth At-lantic rim, with countries succeeding one another in acquiring as-cendancy, then having to relinquish it.Northeast Atlantic fisheries In the first millennium, the coastline of ... collapsed. 21 The Norwegian fishing fleets moved further offshore following the herring collapse. Inthe early 19 70s, they developed fisheries for North Sea mackerel and herring, for shrimp inthe Barents...
... teach how to find the precoordinated string than to teach how to think up all ofthe individual facets thatneed to go into a Boolean combination.) Increasing the granularity of searching tokeyword ... substitute it in place of some ofthe other eight. The scheme starts with a discussion of two particular databases, ReferenceUniverse and Web of Science, because of their utility in zeroing in an overviewencyclopedia ... relegated to the condition ofthe Six Blind Men, enabled to grasp only isolated parts ofthe elephant”without having any mechanism enabling them to perceive the connections of those partsto other...