... indicate the positionand orientation ofthe primers. Bold fontrepresents the sequence of mature miR9*and italic fonts the sequence of maturemiR9. (C) RT-PCR analysis of pri-miR9s dur-ing the ... FEBS 1343 bp ofthe upstream region ofthe pri-miR9-1 fromhuman genomic DNA was cloned and fused into a pro-moterless reporter vector, pGL3_Basic, which contained the ORF ofthe FLuc reporter gene ... vector. The pro-moter sizes ofthe construct are indicatedby numbers in parentheses. )44 meanslocated 44 bp upstream ofthe rst base pairat the 5Â-end of pre-miR-9-1 dened as +1.FLuc is the...
... G4and the sidechain of Asn122. In the second model(Fig. 1B) the structure ofthe hexanucelotide is, on the whole, unchanged and the majority ofthe interactions thatwere seen in the first model are ... attributed solely to the change incharge and size ofthe sidechain of this single residue, the crystal structure of RTA N122A was solved. The RMSDfrom the Caatoms ofthe wild-type RTA crystal ... and, as such, these mutantscould not be purified. To assess the effect of substitutionsmade at each ofthe arginyl residues on catalytic activity of RTA, the ability of each ofthe purified RTA...
... Bp the coast migrated westward with a rate of 75m/year. This means that sea-level rising during the Holocene was not the same. During the Early Holocene sea-level rose slower than in the ... from 100 to 3000m. They distributed parallel to the shore in the form of bows with a distance from each other about 3-10km. They are evidences of paleocoast existence during delta progradation ... mainly of sand (60-80%), silty clay and mollusc, therefore its colour is brownish yellow-typical colour of oxidized environment [11,12]. The elevation of these sand ridges is about 2-7m, their...
... 48 billion during the first half of 2011, slightly behind the EUR 55 billion recorded in the first half of 2010. Long-term UCITS attracted EUR 87 billion during the first half ofthe year, down ... this segment ofthe business accounted for just over 73 percent of the fund market at end June 2011, with the remaining 27 percent composed of non-UCITS. Table 7. Net Assets ofthe European ... billion during the second quarter. Non-UCITS assets have increased by 2.2 percent over the first half of 2011. Net assets of special funds have increased by 1.2 percent during the quarter...
... assets of balanced funds reduced by 2.1 percent. Funds of funds registered an increase in net assets of 1.7 percent during the quarter. Over the first half of 2012, total net assets of UCITS ... this segment ofthe business accounted for almost 71 percent ofthe fund market at end June 2012, with the remaining 29 percent composed of non-UCITS. Table 7. Net Assets ofthe European ... assets of 2.2 percent and 3.3 percent, respectively. Elsewhere, Malta recorded strong asset growth of 32 percent during the quarter due to an increase in the number of authorized funds during the...
... between the projection ofthe tilt ofthe z axis on the xÂ,y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location ofthe rhombic (x, y) axes. The orientation ofthe ... relative to other more autoxidizable globins. Areliable indicator ofthe dynamic stability of distal hemepockets in globins is the rate of reorientation ofthe aromatic rings in the heme pocket ... expected. Aplot ofthe effect ofthe angle, w, between the Tyr32(B10)Cf-OH and ring planes, on the three distinctive variablesthat depend critically on the orientation ofthe OH groupis...
... affected the level ofthe mRNAfor the other ADAM, confirming the specificity of theseASOs [19]. The ASOs reduced the level of their respectiveproteins in cell lysates but had little effect on the otherprotein ... IC50 of 80 nM[14], we considered whether the APMA-induced shedding of ACE was mediated by TACE. The effect ofthe TACE ASO, along with the ADAM10ASO, on the APMA-induced shedding of ACE wastherefore ... in the presence or absence of APMA. In the majority of cases there was little differencebetween the effect of inhibitors on the constitutive shedding of ACE and the APMA-induced shedding of...
... wake at 6 a.m., but nap for two hours or so in the early afternoon. Thus the influence on one's sleep pattern is worthy of consideration when choosing an occupation. ...
... men maintain approximately the same number of hours of unpaid work as in childless households, but the number of hours women work in the home rises to 52 per week, much of it, on doubt, due to ... responsibilities. Interestingly, when there are three or more children in the household, men are found to work even fewer hours around the house than before the appearance ofthe third child. Whereas women's ... answer: The diagram reveals that the number of hours per week spent in unpaid work is unequally distributed between men and women. In households where there are no children, women are reported...
... product, then adjustments will have to be made to the product's design. Similarly, market research, which examines the extent and nature ofthe demand for products, has the role of guiding ... not only the result of product research, but is also influenced by testing and market research. If the testing stage (after assembly and inspection) reveals unacceptable problems in the finished ... serves to foster future sales by devising suitable advertising for the goods. Thus the reality of consumer goods manufacturing goes well beyond a simple linear production process. ...
... into the vas deferens [60], with a follow-up report claiming detection ofthe transgene in the cells of 7.5% of offspring animals produced following fertilisation with the transgene-bearing ... contributed to the embryo, the resultant offspring will be chimeric (up to ca. 50% of animals). The ESC contribution to a mouse can high (up to ca. 80% ofthe cells), and will often include the germline ... provide evidence of post-fertilisation transfer and maintenance of transgenes. Several ofthe studies reportthe subsequent generation of viable progeny animals, the cells of which contain...