list of human diseases caused by bacteria and viruses
Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
Ngày tải lên :
20/02/2014, 02:21
... His120 and His145, and the
corresponding His101 and His126 in arginase I, were
described among the ligands for coordination of
Mn
2+
A
and Mn
2+
B
, respectively [9,24]. However, the
volume of the ... the interaction of human arginase II
with substrate and metal ions was examined by site-
directed mutagenesis and kinetic studies. Selection of
target residues (Asp149, His120 and His145) was ... protein
band was detected by SDS ⁄ PAGE and Coomassie blue
staining of purified enzymes.
Metal-free species of purified enzymes were obtained by
incubation for 1 h at 25 °C with 25 mm EDTA and 3 m
V....
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Báo cáo khoa học: Inhibition of recombinant human maltase glucoamylase by salacinol and derivatives pdf
Ngày tải lên :
07/03/2014, 12:20
... providing lead candi-
dates for the treatment of Type 2 diabetes [23]. In
order to examine the mechanism of action of this
class of inhibitors, 3, 5, the stereoisomers of salacinol
6, 7 [25], and the ... four-carbon chain of salacinol and
the seven-carbon chain of kotalanol.
Fig. 2. Schematic diagram of MGA protein
organization and expression construct.
Amino acid boundaries of each of the
domains ... method of action of acarbose is quite
complex and it appears to be acting as a type of sui-
cide inhibitor of a-amylase in a mechanism whereby
the acarbose is rearranged into an active entity by...
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Báo cáo khoa học: Transcription factor specificity protein 1 (SP1) and activating protein 2a (AP-2a) regulate expression of human KCTD10 gene by binding to proximal region of promoter pot
Ngày tải lên :
30/03/2014, 02:20
... structure of human and
mouse KCTD10 genes, and the 5Â-anking
region sequence of human KCTD10.
(A) Genomic organization of human and
mouse KCTD10 genes. Solid boxes indicate
coding region, and open ... Alignment of the 5Â-anking
sequences upstream of the start codon of
human and mouse KCTD10.
Fig. 5. In vivo ChIP assays of Sp1.1 and AP-2.2 binding sites in the
proximal promoter region of human ... transcription, respectively.
Roles of Sp1 and AP-2a in the regulation of the
promoter activity and the expression of human
KCTD10
To test the effects of Sp1 and AP-2a binding on
KCTD10 promoter...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
Ngày tải lên :
19/02/2014, 07:20
... 3, 4, and 5 were isolated by HPLC and used as substrates for AUH. The AUH assay contained 2 m
M of
the respective isomers of 3-MG-CoA in a total volume of 25 lL. The reaction was started by addition ... CoA-esters were separated by HPLC, ionized by ESI
and detected by TOF. The HPLC system consisted of a
HP1100 series binary-gradient pump, a vacuum degasser (all
from Hewlett-Packard), and a CTC HTS PAL ... procedures
Production and purification of human AUH
in E. coli
The production of AUHp40 (precursor form), AUHp32
(mature form), AUHp40A240 V and AUHp32A240 V in
E. coli was performed using the pMAL-c2 bacterial...
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Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx
Ngày tải lên :
20/02/2014, 11:20
... in
Materials and methods. Results are the mean and standard deviation of iron internalized by normal and iron-loaded hepatocytes from three
separate experiments, and are expressed as a percentage of the ... determine the effect of
chelators on uptake, intracellular distribution and mobiliza-
tion of NTBI; (c) to assess and compare the activity and
toxicity of novel tetradentate and hexadentate pyridinones
with ... overload
conditions, and is also implicated in intracellular Fe
metabolism [3,9,10]. An important aspect of this work
was the assessment of the effect of novel Fe chelators on the
uptake and fate of NTBI and...
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Tài liệu Báo cáo khoa học: Oxygen-dependent regulation of hypoxia-inducible factors by prolyl and asparaginyl hydroxylation pdf
Ngày tải lên :
20/02/2014, 23:20
... Fellow supported by the
Cancer Council of South Australia, and this work was also supported
by the National Heart Foundation and National Health and Medical
Research Council of Australia.
References
1. ... CAD of HIF-1a and HIF-2a.
Other mechanisms of activation
The induction of HIF activity by well established agents
such as hypoxia, cobaltous ions and iron chelators can be
easily explained by ... neuronal cultures by iron chelators is associated with
enhanced DNA binding of hypoxia-inducible factor-1 and ATF-1/
CREB and increased expression of glycolytic enzymes, p21
(waf1/cip1), and erythropoietin....
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Tài liệu Báo cáo Y học: Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins doc
Ngày tải lên :
22/02/2014, 07:20
... isoforms of Sp3 exist and that these
different isoforms play different roles in the activation and
repression of transcription [9]. In our hands, Sp1 generated
a single band and Sp3 generated two bands ... are
similar and binding of these factors is independently
regulated by different coactivators in a cell-cycle-dependent
manner. The distinct functions of Sp1 and Sp3 in the
regulation of expression of ... progression of the cell
cycle and the differentiation of cells [11,12].
The methylation of DNA plays a role in the regulation
of gene expression [13,14], genomic imprinting [15] and
inactivation of the...
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Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
Ngày tải lên :
07/03/2014, 16:20
... The plots of the inverse time constant
(k
obs
) are linear (Fig. 9) and the values of k
off
and the
association-rate parameter, k
on
, listed in Table 3, were
determined as the intercept and the ... , A and k are
the amplitude and the observed rate constant, respectively,
and F
1
is the fluorescence at infinite time. The validity of
the fitting was evaluated by an inspection of residuals and
normalized ... nuclear magnetic resonance study of the cyclic
AMP receptor protein (CRP): assignments of the NH
protons of histidine and tryptophan residues and the
effect of binding of cAMP to CRP. Biochem J 107,
304–309.
14...
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Báo cáo Y học: High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin zinc-finger like sequences doc
Ngày tải lên :
08/03/2014, 23:20
... cofactor for
L. donovani interaction with ECM and BM. Both Zn
2+
and
free sulfhydryls may be required for LBP binding site on
laminin a s e videnced by the stimulatory and inhibitory
effects of ... NaCl/P
i
and immediately f rozen
in liquid nitrogen. Cells were lysed in 100 lLofSDS/PAGE
sample buffe r by boiling for 5 min, p roteins were resolved
by means of 7.5% S DS/PAGE a nd analysed by immuno-
blotting ... for 30 min and harvested
by centrifugation for 10 min at 2000 g. Cells were solubi-
lized by boiling in SDS sample buffer for 5 min and the
extracted proteins were resolved by means of 7.5% SDS/
PAGE...
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