lipid accumulation in silicon deficient diatoms
Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
Ngày tải lên :
19/02/2014, 16:20
... intracellular lipid metabolism, we examined lipid content in iron-loaded HepG2 cells by Oil Red staining Faint lipid accumulation was observed in HepG2 cells grown in normal media (Fig 6B) In marked ... the increase in the prooxidant state caused by iron loading has an impact in protein integrity, as indicated by an increase in protein-bound acrolein adducts at the cell surface, which point ... ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick lines, + saponin) cells cultured without and with iron as indicated (D) Perls’ staining of cytospins...
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Báo cáo khoa học: Fatty acid composition of chylomicron remnant-like particles influences their uptake and induction of lipid accumulation in macrophages pot
Ngày tải lên :
16/03/2014, 12:20
... receptor family in its lipid- free state The receptorbinding region is in an amphipathic a-helix which is part of a four-helix bundle structure in the N-terminal domain, and interaction with lipid is ... setting 22–24 lm, 20 min, at 56 °C) of a lipid mixture containing 70% TG extracted from oils as above, 2% cholesterol, 5% cholesteryl ester and 25% phospholipids in 0.9% (w ⁄ v) NaCl in Tricine ... protocol THP-1 monocytes were maintained in suspension in RPMI 1640 culture medium containing 10% fetal bovine serum, 100 UÆmL)1 penicillin, 100 mgÆmL)1 streptomycin and 50 lm 2-mercaptoethanol...
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Báo cáo khoa học: Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages and increases lipid accumulation in the cells ppt
Ngày tải lên :
16/03/2014, 16:20
... these compounds against cardiovascular disease [11] Dietary lipids, including fats, cholesterol and lipid- soluble antioxidants, are absorbed in the intestine and secreted into lymph in chylomicrons, ... used in our experiments did not exceed 10 lM Culture of THP-1 cells THP-1 monocytes were maintained in suspension in RPMI1640 containing 10% fetal bovine serum, mM glutamine, 100 UÆmL)1 penicillin, ... radioactivity by liquid scintillation counting using Lumagelsafe scintillant (Perkin Elmer Life Sciences) Preliminary experiments showed that [3H]oleate incorporation into lipids was linear over a period...
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Application and Comparison of Two Biotic Ligand Models Predicting Copper Toxicity and Accumulation in Heavy Metal Tolerant Moss
Ngày tải lên :
05/09/2013, 10:15
... flux non-competitively by binding to a binding site other than BL In addition, changes in the conformation of BL caused by this binding of proton result in decreases in the number of BL The conformation ... copper accumulation were fitted to eq using KaleidaGraph ver 4.0 In order to predict the copper accumulation in the moss while taking the metal speciation and the effects of competing cations into ... for the toxicity model, indicating that increases in proton concentration cause decreases in copper toxicity though proton is not a competitive cation with copper binding onto BL Heijerick et...
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Tài liệu Báo cáo khoa học: Proteolytic processing regulates pathological accumulation in dentatorubral-pallidoluysian atrophy pdf
Ngày tải lên :
15/02/2014, 01:20
... role in DRPLA Processing ATN1 in DRPLA neurodegeneration It is tempting to speculate that blocking caspase activity may also inhibit the shuttling system of ATN1, resulting in cytoplasmic accumulation ... aggregation: role in polyglutamine-induced disease in SCA1 transgenic mice Cell 95, 41–53 Saudou F, Finkbeiner S, Devys D & Greenberg ME (1998) Huntingtin acts in the nucleus to induce apoptosis ... demonstrated that an inhibitor of caspase-3 activity produced no reduction in the accumulation of C-terminal F2 fragment Interestingly, the general caspase inhibitor Z-VAD-FMK increased the accumulation...
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Tài liệu Báo cáo khoa học: Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation in proteins with a role in cell–cell interaction pptx
Ngày tải lên :
20/02/2014, 01:20
... Glutamine incorporation in Caco-2 proteins determining the relative uptake of glutamine; (b) by searching for changes in the intestinal proteome; and (c) by examining glutamine incorporation into ... labelling of 20 distinct proteins, which are indicated in bold NQ, No glutamine-containing peptides in spectrum peaks C-term, C-terminal part of protein; N-term, N-terminal part of protein Spot ... synthesis of arginine plays an important role in maintaining arginine homeostasis in postweaning growing pigs J Nutr 127, 2342–2349 61 Quan J, Fitch MD & Fleming SE (1998) Rate at which glutamine enters...
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Tài liệu Báo cáo khoa học: Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc
Ngày tải lên :
20/02/2014, 03:20
... ASS, argininosuccinate synthase and ASL, argininosuccinate lyase by arginine [16,17] As a result, the concentration of citrulline and arginine is kept low through these feedback inhibitions in the ... [15,30,31], and ornithine is exported to the cytosol to be converted into citrulline and arginine [31,32] In plants, information on the localization of citrulline and arginine biosynthetic enzymes ... These findings suggest that citrulline functions as a hydroxyl radical scavenger in the presence of strong light In addition to citruline, concentration of arginine increases from 0.3 mm in unstressed...
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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
Ngày tải lên :
20/02/2014, 11:20
... with aging (Eur J Biochem 270) 2299 Western blotting of modified proteins Fig Determination of CML-protein content in liver mitochondrial matrix with aging The CML content in the matrix proteins ... efficiency in relation to damaged protein and/or a decrease in the amount of this protein in the matrix We have not detected any significant modification in the amount of this protein in the matrix ... proteins and their immunological signals increased with aging These findings are consistent with previous reports showing an increase in carbonyl proteins in mitochondria from different tissues...
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Báo cáo Y học: Formation of conjugated D11 D13-double bonds by D12-linoleic acid (1,4)-acyl-lipid-desaturase in pomegranate seeds doc
Ngày tải lên :
08/03/2014, 16:20
... cloned into yeast expression vectors under the control of the inducible GAL1 promoter and the encoded proteins were expressed in S cerevisiae strain INVSc1 In induced cultures of cells harboring ... linolenic acid isomers is determined Plant oils containing conjugated linolenic acid derivatives are of commercial interest, since they are used as drying oils in paints Thus seed oils may be useful ... temperatures This was in contrast to the increased accumulation of linoleic acid and hexadecadienoic acid in cells expressing Ó FEBS 2002 Punicic acid producing (1,4)-acyl -lipid- desaturase (Eur...
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Báo cáo khoa học: Lipins from plants are phosphatidate phosphatases that restore lipid synthesis in apah1Dmutant strain of Saccharomyces cerevisiae ppt
Ngày tải lên :
15/03/2014, 00:20
... mammalian lipins Based on previous studies demonstrating the involvement of yeast PAH1 and mammalian lipins in storage lipid accumulation [27], we were initially interested in identifying Arabidopsis ... v) raffinose, 20 mgÆL)1 adenine, arginine, tryptophan, methionine, histidine and tyrosine, 30 mgÆL)1 lysine and 100 mgÆL)1 leucine The cultures were grown at 30 °C in a rotary shaker at 250 r.p.m ... of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Jamal Z, Martin A, Gomez-Munoz A & Brindley DN ˜ (1991) Plasma membrane fractions from rat liver contain a phosphatidate...
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A review on the cd content of rice daily cd intake and accumulation in the kidneys
Ngày tải lên :
15/03/2014, 23:11
... residents in an area contaminated by irrigation water in China Bull World Health Organ 1995; 73: 359–367 25) Rivai IF, Koyama H, Suzuki S Cadmium content in rice and rice field soil in China, Indonesia, ... in daily intake of cadmium and its accumulation in the kidneys for example in Germany and Sweden individuals and total diet method for populations Foods and beverages are sampled and bought in ... by using a nomogram indicating the relation between the average concentration of cadmium in the kidneys at age 50 and the average daily cadmium intake in an area or a country Daily cadmium intake...
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Báo cáo khoa học: Brain lipid composition in postnatal iron-induced motor behavior alterations following chronic neuroleptic administration in mice potx
Ngày tải lên :
16/03/2014, 13:20
... stress in the normal and parkinsonian brain Ann Neurol 32, S1–S145 Strong R, Mattamal M & Andorn A (1993) Free Radicals in Aging In Free Radicals, the Aging Brain, and Brain lipid composition in ... four week administration of chlorpromazine, trifluoperazine, haloperidol and sulpiride Fluphenazine and thioridazine caused an increase of PLA2 activity in rat brain both after a single dose and ... was also shown by Singh et al that haloperidol reduced biosynthesis of various phospholipids in rat brain by following incorporation of 32P into individual classes of phospholipid [32] The traditional...
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A simple route to annihilate defects in silicon nanowires
Ngày tải lên :
16/03/2014, 15:03
... the Si crystal core in the tip also has a high density of stacking faults and micro-twins, along the axis of the nanowire in the [1 2] growth direction (indicated by the arrow) In our observation, ... the defects inside the SiNWs were signi®cantly decreased after the SiNWs were thermally annealed at 1100°C for h under an inert ambient HRTEM investigation of the microstructures in the nanowires ... con- Fig TEM morphology of annealed SiNWs The inset is a SAED pattern Fig Typical HRTEM images of SiNWs: (A) an as-grown SiNW with defects; (B) an annealed SiNW without defects 348 Y.H Tang et...
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Efficient indirect exciton luminescence in silicon nanowires
Ngày tải lên :
16/03/2014, 15:15
... fast radiative recombination process is dominant The temperature dependence of the integrated PL intensity in the nanowire is completely di erent from that in bulk c-Si Considering the weak temperature ... Acknowledgements –1 10 PL Intensity (arb units) PL Intensity (arb units) 10 agreement with that of the integrated intensity, compared with that in bulk c-Si This agreement suggests that in the nanowire ... that in bulk c-Si The lifetime of the slow component is comparable with that in bulk c-Si Fig shows the temperature dependence of the fast-decay PL intensity and the integrated PL intensity in...
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Interplay between phonon confinement effect and anharmonicity in silicon nanowires
Ngày tải lên :
16/03/2014, 15:17
... by starting from the silicon crystalline material (eching of the crystalline bulk sample) so the amorphous signal is expected to be much smaller The Raman spectra of silicon nanowires point to ... feature is suppressed in nanowires in comparison to nanoclusters suggests that it might originate from the Raman scattering of amorphous silicon This can be understood as being the consequence ... showing that the shift and broadening arise mainly due to local laser heating effect The expected peak asymmetry, due to phonon confinement effect, is not observed Similar results are obtained in...
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Báo cáo khoa học: Theoretical study of lipid biosynthesis in wild-type Escherichia coli and in a protoplast-type L-form using elementary flux mode analysis potx
Ngày tải lên :
22/03/2014, 21:20
... decarboxylated again (Fig 2) The remaining EFMs are capable of producing all of the main metabolites that we are interested in Obviously, in order to produce one of the forms of lipid A or phospholipids, ... core EFMs producing one of the two forms of lipid A comprise 24 EFMs in the case of lipid A and 51 for lipid A (ca) In addition, there are a number of side EFMs forming lipid A [or lipid A (ca)] ... L1-phosphatidylethanolamine (L1-P-EtAmine), and cardiolipin An example of a core mode is an EFM that produces lipid A without using any of the other products of interest, namely lipid A (ca), L1-P-EtAmine, or...
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Báo cáo khoa học: Role of gangliosides in the association of ErbB2 with lipid rafts in mammary epithelial HC11 cells docx
Ngày tải lên :
23/03/2014, 10:21
... observed (Fig 4C,E respectively) These findings support the view that GM3 is mainly involved in retaining ErbB2 in lipidraft domains, but that it is not involved in ErbB2 phosphorylation EGFR and Shc-p66 ... specifically interact with the purified recombinant extracellular domain of EGFR [22] and that this tyrosine kinase receptor contains a structural domain with targeting information for lipid domains [23] ... a key role in retaining ErbB2 and proteins correlated with its signaltransduction pathway in lipid rafts A B C D Results ErbB2–GM3 colocalization in mammary epithelial HC11 cells In order to...
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Báo cáo khoa học: Detergent-resistant membrane fractions contribute to the total 1 H NMR-visible lipid signal in cell potx
Ngày tải lên :
23/03/2014, 17:21
... acyl chains of PtdCho or sphingomyelin; however, neither the choline headgroups of PtdCho and sphingomyelin (except for a small peak in THP-1) nor the sphingosine chain of sphingomyelin were ... giving it characteristics of being in the liquid-ordered (lo) phase [10] DRMs are rich in cholesterol and sphingolipids (including GM1 and GM3) and selectively retain a number of proteins including ... crosslinking leads to an increased partitioning of TCR subunits and their associated signalling molecules into DRMs implicating their central role as a focal point for T cell activation Interestingly,...
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High-Tech Start-Ups and Industry Dynamics in Silicon Valley potx
Ngày tải lên :
29/03/2014, 23:20
... doing business in Silicon Valley, state and local governments should encourage firm founding Offering favorable tax breaks, opening industrial parks, building high-tech incubators, and providing ... for “moving inside” Silicon Valley, which is not a major concern of our study Death Merger and acquisition Moving inside Moving out Moving in Growth Birth Figure 1.2—Industry Dynamics in Silicon ... Relocating Into Silicon Valley, 1990–2001 4.5 Top Ten Origin Cities for Establishments Relocating Into Silicon Valley, 1990–2001 4.6 High-Tech Establishments Relocating Into and Out of Silicon...
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characterization in silicon processing strausser usa 1993 ebook
Ngày tải lên :
04/06/2014, 13:14
... transistor, a polysilicon or polycide interconnect line in any IC, in a solar cell, or in a thin-film transistor in a display device In order to control resistivity in the final product, one ... the grain size distribution because the entire grain outline can be seen and more grains can be included in one image, improving the statistics Depending on which thin slice of the original film ... typically done in a long quartz tube capable of holding many wafers at a time, although there is increasing interest in single-wafer deposition using a rapid thermal (RTCVD) process Depending on pressure,...
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