lin 12 notch signaling induction lateral specification and interaction with the egf ras pathway
báo cáo hóa học: " Signaling pathways mediating a selective induction of nitric oxide synthase II by tumor necrosis factor alpha in nerve growth factor-responsive cells" pptx
Ngày tải lên :
19/06/2014, 22:20
... were then treated either with TNFα and NGF alone, or with both TNFα and NGF As expected, the combined TNFα/NGF treatment induced a robust expression of iNOS in these PC12 cells, regardless of the ... suggests that signaling from p75NTR in combination with TNFα is necessary to induce iNOS expression On the other hand, our previous work illustrated the importance of TrkA-associated signaling in mediating ... of TNF-promoted signaling occurring selectively in the presence of the glutamate agonist NMDA [4] illustrate the importance of considering the signaling "context" when studying the effects of...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
Ngày tải lên :
14/02/2014, 19:20
... for the very-KIND-MAP2 interaction v-KIND KIND2 KIND2-1 KIND2-2 KIND2-3 KIND2-4 KIND2-5 A 620 521 555 488 589 EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 ... cloned into pGEX-4T-2 The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were ... In the present study, we determined the structural and functional properties of the protein–protein interaction between v-KIND and MAP2 We defined the binding core regions for the v-KIND–MAP2 interaction...
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Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx
Ngày tải lên :
23/03/2014, 06:20
... in the nuclear speckles is associated with sumoylation, we therefore determine whether cAMP signaling affects the SUMO modification pathway We used RT-PCR to study the effects of forskolin on the ... important signaling for regulating the dynamics of the sumoylation pathway and LRH-1 activity in granulosa cells Results LRH-1 is modified by SUMO-1 in vitro To examine whether LRH-1 can be modified with ... forskolin treatment, suggesting that activation of the cAMP pathway may have a positive regulatory effect on LRH-1 activity In response to pituitary gonadotropins, the cAMP signaling pathway is the...
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báo cáo hóa học: " Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury" potx
Ngày tải lên :
19/06/2014, 22:20
... presented in the manuscript, and participated in the writing of the manuscript QC participated in the design of the study and the preparation of the animal model JRK prepared all the cryostat ... gelsolin (pg/ml) after exogenous gelsolin administration in mice after burn injury group 0h 8h 24 h 48 h 72 h control 129 4 ± 113 129 5 ± 81 127 6 ± 77 129 7 ± 69 122 9 ± 50 sham 129 5 ± 111 127 3 ± ... to the injury site, which in turn, slows the clearance of myelin debris and consequently remyelination [22] Consistent with these findings, we noticed that gelsolin infusion could accelerate the...
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Báo cáo hóa học: " Resveratrol engages AMPK to attenuate ERK and mTOR signaling in sensory neurons and inhibits incision-induced acute and chronic pain" pptx
Ngày tải lên :
21/06/2014, 19:20
... 3A) and a reversible inhibition of both ERK (Figure 3B and C) and mTOR signaling (Figure 3D–H) Hence, resveratrol dose- and time-dependently activates AMPK and inhibits ERK and mTOR signaling ... efficaciously inhibits ERK and mTOR signaling in sensory neurons in vitro The mTOR [39-42] and ERK pathways [43] have been linked to pathology in multiple preclinical pain models and our previous findings ... strongly implicate these pathways in the induction of mechanical allodynia by IL-6 and NGF [17,44] and the development and maintenance of nerve-injury induced allodynia [18] The pharmacological...
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Báo cáo y học: "Activation of WNT and BMP signaling in adult human articular cartilage following mechanical injury" potx
Ngày tải lên :
09/08/2014, 08:22
... gene expression analysis of the WNT target genes Axin-2 [31] and c-JUN [32,33] Consistent with our hypothesis and with the activation of the WNT/β-catenin signaling pathway, Axin-2 mRNA was upregulated ... (injured knee) The patellar dislocation was then reduced and the joint capsule and the skin sutured in separate layers The mice were then allowed to walk freely in standard cages and maintained ... which then is degraded through the proteasome pathway When WNT ligands bind to their receptors (called FRZD), GSK-3 is inhibited and β catenin is, therefore, stabilized and accumulates in the cytoplasm...
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Báo cáo khoa hoc:" VEGF receptors on PC12 cells mediate transient activation of ERK1/2 and Akt: comparison of nerve growth factor and vascular endothelial growth factor" doc
Ngày tải lên :
11/08/2014, 08:20
... http://www.jnrbm.com/content/5/1/8 lines, PC12 and CPAE cells were incubated with AP fusion proteins The AP-mVEGF164 affinity probe strongly stained proliferating and differentiated PC12 cells (Fig 1a, b) In contrast, AP-mVEGF110 ... of PC12 cells from apoptosis through VEGF165 is likely also due to its short-lived and relatively small effect on ERK1/2 and Akt VEGF-induced neuroprotective signaling via VEGFR-2, NRP1 and the ... of VEGF and endostatins To determine the expression profile of binding partners for murine VEGF164, endostatin and the collagen XV endostatin homologue on neuronal and endothelial cell PC12 cells...
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Báo cáo y học: "Global analysis of alternative splicing regulation by insulin and wingless signaling in Drosophila cells" ppt
Ngày tải lên :
14/08/2014, 21:20
... insulin and wingless signaling pathways in Drosophila S2 cells Figure Activation of insulin and wingless signaling pathways in Drosophila S2 cells (a) Schematic representation of the insulin and ... deployed by these signaling pathways (see Discussion) Signal-regulated alternative splicing as another level of pathway regulation and crosstalk between pathways Signaling by the wingless pathway ... understanding of the response to these and other signaling pathways will require exploring both transcriptional and post-transcriptional regulation Another relevant case can be made for the alternative...
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Báo cáo khoa học: "Immunohistochemical Localization of Nerve Growth Factor,Glial Fibrillary Acidic Protein and Ciliary Neurotrophic Factor in Mesencephalon, Rhombencephalon, and Spinal Cord of Developing Mongolian Gerbil" ppsx
Ngày tải lên :
07/08/2014, 15:20
... PND2 However, the GFAP-intensity decreased in the forebrain as the fetus developed This is in contrast to that observed in the cerebellum and spinal cord Therefore GFAP within the intermediate ... developing the glia at the early postnatal stages in gerbils CNTF-IR neurons and the glia were widely distributed throughout the rat and mouse CNS and are known to prevent the ‘programmed’ death of the ... in the neurons that developed to their fibers and the somata in the central nervous system (CNS) The GFAP-IR was observed in small numbers in the cortex, for example, the cerebral corticle, the...
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Báo cáo khoa học: "In vitro and in vivo gene therapy with CMV vector-mediated presumed dog b-nerve growth factor in pyridoxine-induced neuropathy dogs" pot
Ngày tải lên :
07/08/2014, 23:22
... were maintained until the end of the pyridoxine injection On the other hand, all of the dogs in the negative control group and the gene therapy group were normal during the postural reaction ... outgrowth, while the PC12 cells in the negative control group maintained their original morphology (Fig 2) Fig PC12 cells were cultured with and without the filtered supernatant and photographed ... and four dogs were in the experimental group with gene therapy All of the dogs were clinically judged to be in good health and neurologically normal, and had their own admission number from the...
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Báo cáo y học: " Aging, osteoarthritis and transforming growth factor-β signaling in cartilage" pps
Ngày tải lên :
09/08/2014, 07:20
... 2, 3, 4, 6, and 7, and phosphorylated Smad (Smad-2P) were localized in the joints of young and old mice In 2-yearold mice there was a decline in the expression of TGF-β2, TGF-β3 and TGF-β receptor ... [2], the authors examined TGF-β in the knee joints of mice aged months or years The mice were challenged with IL-1 to initiate cartilage catabolism The authors examined the response of the articular ... Research & Therapy Vol No Reddi R-Smads and the C-Smad have a highly conserved Mad homology domains (MH in the amino terminus and MH in the carboxy terminus) References Binding of the TGF-β isoforms...
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Báo cáo y học: "Nerve growth factor and receptor expression in rheumatoid arthritis and spondyloarthritsi" potx
Ngày tải lên :
09/08/2014, 14:21
... blockade of the NGF receptor and their signaling pathway by using receptor antagonists) Our findings prompt further functional as well as clinical studies on the role of neurotrophins and their therapeutic ... for both the given target gene and the G3PDH gene Delta Ct was determined as the mean of the duplicate Ct values for the target gene subtracted by the mean of the duplicate Ct values for the G3PDH ... expression levels of the four neurotrophic ligands and the four receptors as determined in the SF cells are depicted in Table and outlined in detail by scatter plots in Figure (ligands) and Figure (receptors)...
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Báo cáo y học: " Increased expression of matrix metalloproteinase-10, nerve growth factor and substance P in the painful degenerate intervertebral disc" pot
Ngày tải lên :
09/08/2014, 14:22
... paraformaldehyde/phosphate-buffered saline and processed into paraffin wax Five-micron sections from the tissue blocks were cut and stained with haematoxylin and eosin, and the degree of morphological ... particular, two of these MMPs (MMP-7 and MMP-13) target type II collagen and aggrecan and are highly expressed within the NP of the degenerate IVD [12, 17], which correlates with our observations regarding ... to increases in both NGF and MMP-10 and therefore matrix degradation, innervation and nociception Furthermore, our results suggest that there may be differences in the pathways involved in asymptomatic...
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Báo cáo y học: "Functional significance of nerve growth factor and its receptor (TrkA) in inflammatory arthritis" pps
Ngày tải lên :
12/08/2014, 14:21
... Raychaudhuri and Raychaudhuri Arthritis Research & Therapy 2010, 12: 404 http://arthritis-research.com/content /12/ 3/404 Page of Table Unstimulated fibroblast-like synoviocytes of patients with psoriatic ... deviation Nerve growth factor (NGF), TrkA, and p75 data of unstimulated FLSs were compared with NGF, TrkA, and p75 data of FLSs of other groups mentioned in the table a/bSignificantly different from ... factor and receptor expression in rheumatoid arthritis and spondyloarthritis Arthritis Res Ther 2009, 11:R82 Raychaudhuri SP, Raychaudhuri SK: The regulatory role of nerve growth factor and its...
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Báo cáo y học: " Open Access Growth factor signaling in lung morphogenetic centers: automaticity, stereotypy and symmetry" doc
Ngày tải lên :
13/08/2014, 13:20
... (FGF) pathway signaling The figure shows a model describing the interaction of murine Sprouty2 (mSPRY2) with other key signaling proteins in the FGF signaling pathway In the upper panel, the FGF pathway ... such as the binding proteins and the negative Smads in the BMP signaling pathway and the Spry gene family in the FGF pathway Moreover each of these pathways can respectively positively and/ or ... restricted and is widespread in the mesenchyme in contact with the epithelium of the Shh null mutant mouse lung Conversely, local suppression of SHH signaling by the induction of Ptc and Hip at...
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Tài liệu Báo cáo khoa học: Osmotic stress sensing and signaling in fishes doc
Ngày tải lên :
18/02/2014, 16:20
... of these divalent ions are well within the millimolar range over which CaSR operates [11] In the mammalian kidney, CaSR regulates the activity of many other signaling pathways, including pathways ... stress signaling pathways integrated into intracellular signaling networks that control other physiological processes? Euryhaline fishes represent an excellent model for addressing those and many other ... insulin-like growth factor I The effect of these hormones is fine-tuned at the cellular level via adjustment of expression and membrane insertion of the corresponding hormone receptors [39] Signaling...
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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx
Ngày tải lên :
18/02/2014, 16:20
... Osmosensing and signaling in mammalian cell function F Schliess et al [7] On the other hand, hyperosmotic shrinkage prevents insulin-induced hepatocyte swelling and proteolysis inhibition, ... association of CD95 with the EGF- receptor, and phosphorylation of CD95 on Tyr232 and Tyr291 by the EGF- receptor tyrosine kinase activity [15] Although the appearance of CD95 at the plasma membrane ... trigger signaling pathways towards effector sites (‘osmosignaling’) Anisoosmotically exposed cells and tissues were frequently used as a model in order to study osmosensing and osmosignaling By this...
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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
Ngày tải lên :
18/02/2014, 16:20
... of PRP with tissue factor and CaCl2, the P2Y12 ⁄ PI3-K pathway significantly enhances the activation state and, hence, the procoagulant activity of platelets Experiments with inhibitors and PI3-Kc ... P2Y12 signaling is conserved in mouse and human platelets, but also highlight the importance of the PI3-Kb isoform PI3-Kb and not PI3-Kc mediates P2Y12-dependent procoagulant activity of mouse and ... potentiation by P2Y12 signaling is conserved between platelets from man and mouse, despite the different thrombin receptor types employed by these species The data are compatible with the earlier findings...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
Ngày tải lên :
19/02/2014, 16:20
... level of p38 signaling, but are all independent of both Erk1/2 and JNK1/2 signaling pathways The signaling components upstream of the p38 identified thus far suggest a complex cell- and stimulus-dependent ... independent of the Ras signaling pathway In contrast, exposure of the cells to the p38 inhibitor significantly reduced serum-, S1P- and anisomycin-induced CTGF/CCN2 gene expression by 30, 35 and 60%, ... regulated by the GTPase Ras and was implicated in TGF-b-induced CTGF/ CCN2 expression, while members of the Rho GTPase family regulate the JNK and p38 MAP kinases The role of these signaling molecules...
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