... (μM)in PBMC NYAD-2 01 >11 5 >11 5 NYAD-202 30.2 ± 4.32 >11 6 NYAD-203 13 .24 ± 0.5 15 .96 ± 1. 47 virions with NYAD-2 01 prior to infection, treated the target cells before or after infection, or treated ... BaL B PBMC R5 4.73 ± 1. 92 2.23 ± 0.44 89.6 B PBMC R5X4 5. 21 ± 0.87 3.47 ± 0.22 RT-Resistant Isolate AZT-R B MT-2 X4 8.0 ± 1. 27 4.53 ± 1. 19 PR-Resistant Isolate HIV- 1RF/L-323 -12 -3 B MT-2 X4 5.6 ... infectivity [19 , 31- 37] The isolated CTD of HIV- 1 CA, like full-length CA, forms a dimer in solution It has been shown that CTD dimerization is a major driving force in Gag assembly and maturation [10 ,15 ]...
... electrophoresis Numbers in parentheses are minor components of the samples Peptides a-PEC Crystals of a-PEC Peptide 1A Peptide 1B Peptide 1C Peptide Peptide Molecular mass 18 15 1.6 18 18 15 (15 15 15 1.6 16 7.8 ... ammonium peroxodisulfate [17 ] Samples of 1. 5 mL containing 10 15 mgÆmL )1 protein were electrophoresed in the Tris/boric acid buffer system for 2400 VÆh )1 at a constant power of 18 W, at 10 °C and continuous ... the PEC complexes [11 ,12 , 21] The assembly of ‘monomeric’ and ‘trimeric’ complexes is accompanied by a decrease of the photochemistry from 10 0% of the isolated a-PEC to 8% for the ‘trimers’ Naturally,...
... equilibrated for 10 in NaCl/Tris before permeabilization with 0 .1% Triton X -10 0 in NaCl/Tris for 30 at room temperature One hour preincubation was performed in the presence of 1% NGS and 1% BSA to ... (1. 5 lgÆmL )1) [14 ], was applied for 12 16 h at room temperature in NaCl/Tris/ NGS/BSA After washing three times (10 min) in NaCl/ Tris, cells were incubated for h at room temperature with : 10 0 ... Tris/NGS/BSA Grids were then washed three times for 10 with NaCl/Tris, postfixed for in NaCl/Tris containing 1% glutaraldehyde and washed twice for with distilled water Sections were stained for...
... represented by the Minister of Agriculture and Agri-Food Canada Journal compilation ª 2008 FEBS 2407 A 0.5 :1 1 :1 2 :1 3 :1 4 :1 5 :1 0.5 :1 A Chugh and F Eudes 1: 1 2 :1 3 :1 4 :1 5 :1 CPPs and their cargo ... Trembleau A, Brunissen A, Chassaing A & Prochiantz A (19 96) Cell internalization of the third helix of the antennapedia homeodomain is receptor dependent J Biol Chem 2 71, 18 188 18 193 10 Henriques ... mediated by cell-penetrating peptide pep -1 into lipid vesicles and human HeLa cells is driven by membrane electrostatic potential Biochemistry 44, 10 189 10 198 11 Mano M, Teodosio C, Paiva A, Simoes...
... 29 212 80 BD 312 ATCC 29 213 59.6 ± 6.7 28.5 ± 3.8 78 .1 ± 10 .6 0 69.3 ± 10 .0 28.9 ± 6.3 63.3 ± 9.9 0 64.6 ± 6.4 12 .1 ± 2.7 56.0 ± 5.6 0 45.2 ± 14 .3 9.4 ± 3.7 35.4 ± 8.3 0 37.4 47 .1 ATCC 25922 27 .1 ... radiolabelled heparin ( 10 lgÆmL )1, 0.4 · 10 6 cpmÆlg )1) for h at room temperature in 10 mm Tris, pH 7.4 with or without 50 lm Zn2+ The membranes were washed for · 10 in 10 mm Tris, pH 7.4 A Bas 2000 ... different strains of E faecalis, 10 0 lm AHH24 :1 or AHH24:2 were incubated with E faecalis 2374, E faecalis BD 33 ⁄ 03, E faecalis BD 96 ⁄ 03 or E faecalis ATCC 29 212 in 10 mm Tris, pH 7.4 in the absence...
... is catalyzed by the enzyme prolyl-3-hydroxylase (P3H1, EC 1. 14 .11 .7), which has three family members in vertebrates, but characterization of these enzymes is very limited [11 – 13 ] The analysis ... trimer) DS° (JÆ°C )1 mol )1 trimer) Tm (°C)a )3 21 )224 )17 7 )17 0 )15 8 )15 6 )8 81 )5 81 )439 ) 418 )373 )370 52.0 52.8 51. 0 52.6 56.2 55.3 a Tm (°C) ΔS (J·K 1 mole 1 trimer) Temperature (°C) modification lowered ... even for the same tissue and species [15 17 ] This isnot just due to the heterogeneity of the modification, but also to differences in sample preparation for amino acid analysis As a result of this,...
... 34–59 64–85 614 .6 /16 39.7 2287.5 14 57.7 315 7.7 2432.8 Not isolated /16 39 2288/2288 14 57 /14 57 315 8/ 315 8 2433/2433 Fig Comparison of the C18 RP-HPLC chromatograms of the Asp-N digestion of rCP10 (Digest ... for details) Protein rCP10 Mutant CP10 rCP10Dimer GST Fusion Protein Mutant fusion Protein Calculated mass (Daltons) ESI mass (Daltons) 10 ,307.6 11 ,332.7 20, 614 .1 26 ,16 6.6 36,456 .1 37,4 81. 2 10 ,308 ... by rCP10 at 14 .1 (Fig 2A) The disulphide-linked homodimer of rCP10 eluted at 15 .1 followed by GST at 17 .5 (Fig 2A) The ratio of mutant protein to rCP10 was approx 1: 10, and this ratio did not vary...
... with Alzheimer disease-like pathology Proc Natl Acad Sci USA 19 97, 94 :13 287 -13 292 Nussbaum RL, Ellis CE: Alzheimer’s disease and Parkinson’s disease N Engl J Med 2003, 348 :13 56 -13 64 Hintersteiner ... 2007, 8 :10 111 2 13 Bonte FJ, Harris TS, Hynan LS, Bigio EH, White CL: Tc-99m HMPAO SPECT in the differential diagnosis of the dementias with histopathologic confirmation Clin Nucl Med 2006, 31: 376-378 ... 73:443-449 10 Sisodia SS, Price DL: Role of the beta-amyloid protein in Alzheimer’s disease FASEB J 19 95, 9:366-370 11 Lin H, Bhatia R Lal R: Amyloid β protein forms ion channels: implications for Alzheimer’s...
... test tasks is a critical issue no matter whether we develop a new system or buy an existing one – For some systems it is hard to find meaningful test tasks at all • For instance, this is the case ... something wrong on the screen • Missing functionality – If it is impossible to carry out the task, the system isnot fit for use – We classify this as a usability defect – missing functionality • Ease-of-use ... cannot figure out how to it or doesn’t like the system, the system isnot easy to use • This is another kind of usability problem – We classify them according to their severity to the user, for...