lectins cona and uea i by human and cow bovine milk
The 11th form non-english majors’ level of satisfaction with their reading comprehension lessons at phan boi chau specialized upper secondary school, nghe an
... (more like real world conditions) and much more feasible given time and logistical constraints And since this design is less intrusive and disruptive than the true-experiment design, it is easier ... main five specific strategies In addition, designed activities were provided in order to help students understand and use the five specific strategies in CS They are: Formally practicing with ... an individual in a particular learning situation to facilitate their acquisition of knowledge or skill, and to achieve their desired objectives It has been further claimed that CS can be defined...
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THE 11TH FORM NON ENGLISH MAJORS’ LEVEL OF SATISFACTION WITH THEIR READING COMPREHENSION LESSONS AT PHAN BOI CHAU SPECIALIZED UP
... are designed with pre-reading activities, while- reading activities and post reading activities Most pre-reading activities are “using pictures to introduce the topic” Post-reading activities are ... 12 I. 3 Learners’ perceptions/satisfaction in language education .12 II.4 Reading and reading activities 12 II.4.1 Definition of reading 12 II.4.2 Reading activities: ... Learners’ perceptions/satisfaction in language education II.4 Reading and reading activities II.4.1 Definition of reading There are a number of different definitions of reading: According to Harmer...
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Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot
... near to the binding site(s) and their positive charge is a stringent requisite for the binding This is not surprising, if indeed collagen binds inside the concave surface of decorin, owing to the ... decorin and collagens or their CNBr peptides is quite sensitive to the ionic strength of the buffer, suggesting an ionic character of the binding The main decorin region implicated in the binding ... collagens and positive peptides, whereas fibronectin and a 30-kDa fibronectin fragment having heparin-binding ability showed interaction with tendon decorin (Fig 4) Fibronectin is known to interact with...
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Báo cáo khoa học: Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia pdf
... annexins colocalize in the same cilia Figures and show the immunohistochemical studies of dicalcin and annexin A1, annexin A2 and annexin A5 In Fig 4, the olfactory cilia, which were identified immunohistochemically ... dicalcin is stoichiometrically sufficient to form complexes with annexin A1, annexin A2 and annexin A5 Facilitation by dicalcin of membrane aggregation induced by annexins The half-maximal dicalcin ... poly(vinylidene difluoride) membrane (Immobilon P; Millipore, Billerica, MA, USA) After blocking with 10% skimmed milk in NaCl ⁄ Tris, the membrane was incubated with anti-annexin or anti-dicalcin...
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Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx
... Electrophoretic analysis, comparing gel mobility-shift induced by DAPI on the 20 different individual strands of trinucleotide repeats, shows an observable increase in electrophoretic mobility induced by ... changes in DAPI fluorescent properties which are indicative of a weak minor-groove interaction, GÆC-like intercalation or uncharacterized binding mechanisms: the shift in the maximum emission at ... mechanism of action of the classical minorgroove binding ligand DAPI This property is associated with the ability of the drug to bind and stabilize folded structures in ATT-triplet sequences, by...
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Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt
... localization machinery in Xenopus oocytes, tandem affinity purification (TAP)-tagged versions of the known vegetal localization factor Vg1RBP were expressed in stage III and IV X laevis oocytes by ... colloidal Coomassie staining M, protein size marker The bands marked by blue lines were excised and analyzed by MS Putative Vg1RBP binding partners identified are ePAB, FRGY-2B and 42Sp50 Degradation ... proteins strongly stained by Coomassie were 4724 reliably identified by MS analysis Different stoichiometries of individual protein components, which assemble into one RNP with Vg1RBP, as well...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... dissociation constants of ricin and RCA120 have been determined mainly by equilibrium dialysis studies and fluorescence polarization studies [32–42] Ricin has at least two binding sites in its molecule The ... high-affinity sites of ricin and RCA120, whereas Kd for the c sugar-binding site of EW29Ch is very similar to that for the third binding site of the ricin B chain and has the lowest binding ability ... sites in ricin Biochemistry 38, 11677– 11685 24 Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y & Hirabayashi J (2007) Tailoring a novel sialic acid-binding lectin from a ricin-B chain like galactose-binding...
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Báo cáo Y học: Exploration of the diaphorase activity of neutrophil NADPH oxidase Critical assessment of the interaction of iodonitrotetrazolium with the oxidase redox components doc
... reoxidation of heme b initiated by O2 and Ó FEBS 2002 INT reductase activity of neutrophil oxidase (Eur J Biochem 269) 1249 Kinetics of inhibition of the oxidase activity by INT and bipyridyl Fig ... case with bipyridyl as shown in Fig 7B A similar competitive inhibition of O2 uptake by bipyridyl was found when activated neutrophil membranes were incubated with NADPH in an aerated medium (Fig ... reduction of heme b by NADPH After full reduction of heme b with sodium dithionite, further cycles of oxidation were initiated by addition of O2 and INT (respectively 10 and nmol) INT were also significantly...
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Báo cáo y học: "Direct interaction of immunoglobulins with synovial fibroblasts: a missing link in the pathogenesis of rheumatoid arthritis" potx
... Benoist C, Mathis D: A revival of the B cell paradigm for rheumatoid arthritis pathogenesis? Arthritis Res 2000, 2:90-94 Steiner G, Smolen J: Autoantibodies in rheumatoid arthritis and their clinical ... systemic T cell self-reactivity to organ-specific autoimmune disease via immunoglobulins Immunity 1999,10:451-461 Matsumoto I, Staub A, Benoist C, Mathis D: Arthritis provoked by linked T and B ... observations and link IL-16 to the disease process of RA In this context it is of interest, that CD4 expression per se is not sufficient to mediate IL-16 effects [19] Rather, IL-16 mediated T cell migration...
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báo cáo khoa học: " Gene expression profiling in susceptible interaction of grapevine with its fungal pathogen Eutypa lata: Extending MapMan ontology for grapevine" pdf
... transcriptome in a susceptible but not in a resistant grapevine [28] In addition, possible innate resistance against pathogenic fungi is being unravelled using transcriptional and metabolic profiling ... (Vitis vinifera L.) berries during ripening initiation reveal that pigmentation intensity is a valid indicator of developmental staging within the cluster Plant Mol Biol 2008, 68:301-315 Terrier ... for Affymterix microarrays KG was involved in organization of the work and writing of the manuscript Additional material Additional file ID, VVGI5id, VVGI5annotation, UniqueOligo, logFC, AveExpr,...
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Interaction of EcoRI with noncognate DNA sequences computational investigation of dynamics of protein water and DNA conformation
... DNA by a series of microscopic dissociation and rebinding events, the protein is said to exhibit one-dimensional hopping (iii) Jumping In this model, the protein moves over longer distances in ... intriguing aspect in such protein-DNA interactions is how these proteins rapidly identify their target DNA sequences with such Introduction: Protein-DNA Interactions high fidelity The specificity ... the protein and the DNA, contribute to the specificity in proteinDNA interaction In addition, recent works and understanding that biomolecules are dynamic entities and not static entities have...
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Interaction of scribble with zonula occludens and intermediate filament proteins
... PDZ-binding motif and Scrib PDZ domains In addition, GIT1 associates with the GIT1 binding motif (GB) of PIX and forms a tripartite complex with Scrib In neuroendocrine cell-line PC12, interaction ... their epithelial characteristics and become tumorigenic (Wodarz and Nathke, 2007) With such wide ranging physiological functions and implications in human diseases, the understanding of epithelial ... degradation of Scrib is inhibited by its interaction with vimentin Table Direct interacting partners of Scrib Movie MDCK non-targeting control siRNA wound-healing assay Movie MDCK vimentin siRNA...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
... respectively, Fig 1D) This effect might arise either due to steric hindrance imparted by ssDNA binding or to changes in protein configuration ⁄ conformation following ssDNA binding or a combination ... 10–12, 14–16 with of 2–4, respectively, Fig 1A) However, the signals associated with protein–DNA complexes (position indicated by asterisk) appear to diminish and that of higher oligomeric forms that ... 10 with 2–4 and 7–9, respectively, Fig 4B) This was concomitantly associated with the rise of a signal at high molecular weight region in the gel (at asterisk position in lanes and 10) This was...
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Intein mediated generation of n terminal cysteine proteins and their applications in live cell bioimaging and protein microarray
... two strategies for site-specific immobilization of peptides using the high affinity biotin-avidin interaction and native chemical ligation.[44] The use of the biotin-avidin interaction has recently ... Acknowledgements i Table of Contents ii Summary iv List of Figures vi Abbreviations viii Introduction 1.1 Inteins and protein splicing 1.2 Native chemical ligation 1.3 Bioimaging 1.4 Protein microarrays ... DnaB intein-fusion system was designed for in vitro affinity column intein-mediated cleavage and protein purification.[53] This meant cell growth and purification conditions which minimized the in...
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Báo cáo y học: " Antagonistic interaction of HIV-1 Vpr with Hsf-mediated cellular heat shock response and Hsp16 in fission yeast (Schizosaccharomyces pombe)" potx
... using the C-terminal truncated Vpr-carrying viral strain IIIB As shown in Fig 2B-b and Fig 2B–d, the kinetics of viral replication were essentially indistinguishable between cells with or without ... inhibition by Vpr Since the Vpr-specific activities have been linked to such clinical manifestation of AIDS as activation of viral replication [57], suppression of host immune responses [19] and ... Consistent with these activities, Vpr has been shown to increase viral replication to fold in proliferating T lymphocytes [8,39,40] but its activities are required for viral infection in non-dividing...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... substrate l-arginine [11,13] Particularly interesting has been a possible role of arginase II in regulating the availability of l-arginine for nitric oxide synthesis in human penile and clitoral corpus ... Kinetics of inhibition of rat liver and kidney arginases by proline and branched-chain amino acids Biochim Biophys Acta 870, 181–184 26 Tormanen CD (2003) Substrate inhibition of rat liver and ... the active sites of this enzyme and arginase I In spite of the relatively low agmatinase activity of the Asn149Asp variant, it is clear that the interactions of arginase II with l-arginine and agmatine...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... polymerization was initiated by the simultaneous addition of actin nuclei and salts Neither type of HSP25 had any significant effect on the critical concentration of actin (Fig 6A) Indeed the critical ... four different preparations of actin completely prevent actin polymerization In addition, this heating regime resembles that occurring in vivo under certain physiological conditions In preliminary ... polymerization of intact actin and aggregation of heated actin Interaction of HSP25 with intact actin Fig Effect of heating on the kinetics of polymerization (A) and saltinduced increase of the light...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... transcription factor in Bacillus subtilis under nitrogen-limited conditions (conditions in which the nitrogen source becomes growth-limiting), controls gene expression in response to nitrogen availability ... E coli BL21, purified to apparent electrophoretic homogeneity [12], and used in BIAcore analysis on immobilized His6-tagged TnrA immobilized on a chelating nitrilotriacetic acid sensor chip NAGK ... availability During nitrogen-limited growth, TnrA serves either as an activator or a repressor of genes involved in nitrogen assimilation TnrA activates its own gene [1], the nitrate and nitrite utilization...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... superantigens (shown in cyan) Residue Cys26 is shown in red Equilibrium and kinetic parameters for SSA binding to different TCR b chains were determined in a resonance mirror using an IAsys instrument ... binding site in other SAgs Point mutation of Cys26 to Ser prevented dimer formation and allowed TCR interaction studies Initial binding experiments using a biosensor gave an apparent dissociation ... could explain why dimerization mediated by this residue occludes the TCR interaction site This research was supported by Universidad de Buenos Aires; Consejo ´ Nacional de Investigaciones Cientı´...
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Báo cáo khoa học: Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine docx
... considered a more central role for a peroxidase–H2O2 mechanism in initiating melanogenesis [9] In this investigation, extremely sensitive and specific electrochemical methods detected and quantitatively ... used in reaction mixtures to ascertain the involvement of additional ROI during the autoxidation of diphenols In these experiments, varying amounts of dopamine were introduced into reaction mixtures ... 7), into reaction mixtures containing tyrosinase and either DOPA (Fig 8) or dopamine (not shown) produced a slight but statistically significant (P < 0.05) increase in the tyrosinase-mediated oxidations...
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