... communitystructureofaquaticorganismsinthe experimental rice fields inthe presence/absence oflarvaestockingIn addition, the effect of rice- straw plowing onthecommunity was examined collaterally ... clarify the feeding habits of larvae/ fryof C a grandoculis and their impact onthe plankton communityinrice fields To this, we regularly surveyed the gut contents of C a grandoculis andthecommunity ... 10.1007/s12562-009-0200-3 ORIGINAL ARTICLE Biology The impact of nigorobuna crucian carp larvae/ frystockingand rice- strawapplicationonthecommunitystructureofaquaticorganismsinJapaneserice fields Masatsugu...
... immunoreactivity inthe blue crab: Tissue distribution, variations inthe molt cycle, and partial characterization J Exp Zool 262:279-286 Castille Jr F.L and A.L Lawrence 1981a The effect of salinity onthe ... Osmotic and chloride regulation inthe hemolymph ofthe tiger prawn Penaeus monodon during molting in various salinities Marine Biology 95:377-385 Funge-Smith S.J., A.C Taylor, J Whitley and J.H ... comparison of osmotic, sodium and chloride concentrations between the urine and hemolymph of Penaeus seti ferus (L.) and Penaeus stylirostris stimpson Comp Biochem Physiol 70A:525-528 Chen J.C and...
... immunoreactivity inthe blue crab: Tissue distribution, variations inthe molt cycle, and partial characterization J Exp Zool 262:279-286 Castille Jr F.L and A.L Lawrence 1981a The effect of salinity onthe ... Osmotic and chloride regulation inthe hemolymph ofthe tiger prawn Penaeus monodon during molting in various salinities Marine Biology 95:377-385 Funge-Smith S.J., A.C Taylor, J Whitley and J.H ... comparison of osmotic, sodium and chloride concentrations between the urine and hemolymph of Penaeus seti ferus (L.) and Penaeus stylirostris stimpson Comp Biochem Physiol 70A:525-528 Chen J.C and...
... depends on both the concentration of free PMLA andthe inhibition constant This parameter reflected the affinity ofthe polymerase and both the affinity and concentration of competing ligands for binding ... proportional to vi and vo The expression for the relative inhibitory activity is then (vo ) vi)/vi ¼ [PMLA]/Ki Therefore, the higher the concentration of free PMLA, andthe lower the value ofthe inhibition ... polymerase (the arrow in Fig 1) During continued addition ofthe polymerase, the enzyme activity increased in parallel with the activity ofthe control experiment inthe absence of extract An amount of...
... in Mainland China and Taiwan Province of China and subsequently spread to the Philippines, Indonesia, Viet Nam, Thailand, Malaysia and India (RAP, 2004) In 2008, 67% ofthe world production of ... remains until the next molting cycle Duration ofthe molting cycle depends onthe species and age ofthe shrimp Molting frequency on white shrimp decreased along with increasing the size of shrimp ... discrepancies between the ion concentrations within their bodies andtheaquatic environments they inhabit The degree ofthe osmoregulatory response is largely salinity dependent, the actual strength...
... recognition of pNPP by the pNPP site at the rear surface ofthe N-domain is due the formation of an hydrogen bond ofthe NO2-group of pNPP with the amino group of N398 The hydrophobicity ofthe binding ... indication of a conformational change ofthe N-domain upon ligand binding inthe investigated GST fusion protein It is quite evident that the N-domain ofthe isolated loop forms a rigid structure, ... Mapping of ATP and pNPP binding sites ofthe Na pump (Eur J Biochem 271) 3933 Table Comparison ofthe primary structures of isoforms of Na+/K+ ATPase and H+/K+ ATPase inthe range ofthe binding...
... aggregation of lens proteins and enzymes These protective capabilities have been demonstrated with other, in vitro systems, including prevention of aggregation of insulin B chain following reduction of ... assayed following the method of Bernt and Bergmeyer [24], inthe presence of fructose (50 mM) to determine if the inhibitory effect ofthe modifier was through inhibition ofthe ATP-regenerating system ... regeneration system, nor by loss of ATP or by changes in concentrations of Na+ and K+ levels These experiments were performed inthe absence of a-crystallin, suggesting that the chaperone function of...
... dissociation, conformational change of protein, and/ or the destruction of integral structure (lipid bilayer and protein) The issue of clarifying the precise nature ofthe interrelationship between these ... methodology in discriminating conformational changes Effects of pressure on intrinsic ¯uorescence The emission maximum (kmax) and intensity of intrinsic ¯uorescence of Na+/K+-ATPase under increasing ... dissociation of a and b subunits, disassembly of transmembrane a helices, and a separation inthe contact surface of membrane and protein due to the thickening and shrinkage of lipid bilayer For the...
... isoforms in single skeletal muscle fibres to have a more complete understanding ofthe underlying molecular properties and adaptations ofthe NKA following training, inactivity andin ageing In ... Kane and Anthony Denaro in particular for the last months of this thesis finishing for adopting me in to their house, reminding me to eat, sleep and exercise on a daily basis And to the rest of ... Calcium entering the cytoplasm binds to troponin causing tropomyosin to physically shift, uncovering the cross-bridge binding sites onthe actin filament The head ofthe myosin filament can then attach...
... was increased from 0.1 to mm to completely depress the increase inthe ouabain-insensitive activity due to increasing the KCl concentration Onthe other hand, increasing the Na+ concentration ... KCl inthe presence of NaCl with or without oligomycin Inthe absence of oligomycin, increasing the NaCl concentration decreased pNPPase activity (Fig 2A) Inthe presence of oligomycin, increasing ... resultant increase in Na+ binding was regarded as Na+ occlusion Inthe absence of oligomycin, NaCl gradually inhibited pNPPase activity and Rb+ binding (Fig 4A,B) Inthe presence of oligomycin, the binding...
... cells including human cells [7] Targets ofthe kinase inthe regulation of DNA replication include ORC2, Cdc6 and Mcm proteins in Saccharomyces cerevisiae [8], and disregulation of these proteins ... phosphorylation of MCM4, together with the presence of geminin, which inhibits the ability of CDT1 to load MCM proteins onto chromatin, may help prevent re-replication of DNA inthe G2 and M phase During ... during the HeLa cell cycle in a site-specific manner Cyclin-dependent protein kinase is involved inthe phosphorylation of MCM4 To determine which kinase is involved inthe phosphorylation of...
... trimethylation marks were localized inthe less intense DAPI-stained regions inthe nucleus (representing less condensed euchromatin), leaving gaps inthe more intense DAPI-stained regions (representing ... expression profile (as shown in Fig 6A,B), indicating the importance of MLL1 and H3K4 trimethylation in HoxA5 gene regulation during cell cycle progression The association of a certain amount of RNAP ... Fig 6C,D) Inthe case ofthe HoxA7 gene promoter, the recruitment of RNAP II and MLL1 andthe level of H3K4 trimethylation were low at the beginning (S phase) and gradually increased as the cell...
... by the pump The mentioned, often contradictory observations and interpretations not add to the credibility ofthe hypothesis The main objections to a normal signaltransducing function of ouabain ... signal-transduction were interpreted as independent of a small increase in [Na+]i and also occasionally independent ofthe subsequent increase in [Ca2+]i [10], whereas in other publications, activation ofthe ... enzyme consists ofthe ouabain-insensitive a1-isoform [19] This explains why large concentrations of ouabain (50–500 lM) are needed to obtain Ca2+-oscillations in a reasonable fraction ofthe rat...
... inhibition by ouabain initiates the increase in [Ca2+]i, andthe other the signal transducing pool ofthe enzyme which, through protein–protein interactions, leads to the activation of a host of signaling ... one pumping ions /K andthe other interacting with neighboring proteins are suggested by the data The partial inhibition ofthe pump by ouabain causes a modest change, if any, in [Na+]i and [K+]i, ... contain little or no Na+/Ca2+-exchanger, the demonstration ofthe independence ofthe signal transducing role of Na+/K+ATPase using altered intracellular ion concentrations has been easier in these...
... rejection based on minor deviations from the law, whether inherent in minor deviations of cellular structurein all cells or deviations from one cell to another cell Studying the deviations from ... distribution ofthe cells at the time they were filtered The decrease inthe dashed line is placed at the end ofthe first division cycle as indicated by the cell elution curve The decreasing exponential ... The data are indicated by the filled squares The straight line drawn through the points is the best fit based on a minimization of deviation of points from the straight line A straight line on...
... proteins (VP2 and VP3) ofthe MPyV were studied inthe absence of other virus components as well as during the late phase of virus infection The role ofthe minor structural proteins inthe replication ... toxicity ofthe minor proteins is controlled during the infectious cycle and that other viral components and ⁄ or cell responses are involved in cell death during the late phase of viral infection ... VP2 and VP3 andthe fusion variants VP2–EGFP and VP3–EGFP To further examine the membrane localization ofthe cytoplasmic fractions of fusion proteins, the 1272 mutual location of membranes stained...
... sense and antisense strands ofthe piRNA hairpin are involved in formation ofthe piRNA-interacting complex (piRC) and function inthe transcriptional gene silencing of retrotransposons and genetic ... generation to glial cell generation, and to be related to the expression of granuphilin ⁄ slp4 in insulin-producing cells [28] Moreover, miR9 and other neuronal miRs including miR125b and miR128, ... positions and sequences used to amplify pri-miR9-1, 9-2 and 9-3 Arrows indicate the position and orientation ofthe primers Bold font represents the sequence of mature miR9* and italic fonts the...
... binding to the zinc binding sites (M2 and M1) It appears that in contrast to the binding of Zn2+ to the second M3 site, Mg2+ binding inthe range of Mg2+ concentrations examined (if it binds at all ... cooperativity in metal ion binding to the M3 site results in homodimer asymmetry Consequently, thein uence of Mg2+ and Zn2+ onthe kinetic properties of APase andthe deviations from Michaelis–Menten kinetics ... Deviation from linearity inthe dependence ofthe Mg2+ ion concentration It has been determined that the affinity ofthe subunits for the substrate andthe product does not depend onthe Mg2+ concentration...
... protein aggregation occurs during the thermal denaturation of intact SK andofthe isolated A domain The presence of these aggregation processes has a profound effect onthe DSC curves and precludes ... represented in continuous line, second heating in dashed lines, third heating in dotted lines and fourth heating in dashed-dotted lines equilibrium at low concentration are the native, N, andthe unfolded, ... the DSC transitions of intact SK by consecutive heating ofthe same sample inthe calorimeter The sample concentrations and pH values are indicated inthe panels First heatings ofthe sample are...
... provide insights into the origin of its individual enzymes However, the study ofthe enzymes ofthe tricarboxylic acid cycle necessarily also entails the study ofthe several enzymes involved inthe ... compartmentalized inthe glyoxysomes of plants [31], the single exception being aconitase, which is localized inthe cytosol [32,33] Here we investigate the evolution ofthe enzymes ofthe pyruvate ... (1992) The aconitase of Escherichia coli Nucleotide sequence ofthe aconitase gene and amino acid sequence similarity with mitochondrial aconitases, the iron-responsive-element-binding protein and...