kinetics of biological catalysts
The Emergence of Biological Value
Ngày tải lên :
01/11/2013, 08:20
... it is often claimed (e.g., Depew and Weber 1998) that the nor-
mativity of biological functions can be fully naturalized in terms of Wright’s
(1998) analysis, in which a function is a part of a ... sense.
Of course, science often looks to history to explain how the present state
of a system came into being, but the present causal powers of a system
must nevertheless be explicable in terms of ... stability of the oscillator.
This dynamical interpretation of semantic information provides us with a
new physical picture of the cognitive component of adaptive functional
action.
Most, if not all, of...
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Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
Ngày tải lên :
14/02/2014, 14:20
... populations of LDLR that exist
prior to ligand binding (see below).
Previous Biacore studies have primarily used the
ECD of LDLR to determine the affinity and kinetics
of binding of PCSK9. The ... the LDL-binding
domain of LDLR [18].
The molecular basis of the enhanced rate of dissoci-
ation observed in the presence of unlabeled ligand is
unclear. This phenomenon has often, but not always,
been ... characteristics
of binding of PCSK9 to LDLR. Using PCSK9 iodinated by the tyramine
cellobiose (TC) method ([
125
I]TC-PCSK9), we measured the affinity and
kinetics of binding of PCSK9 to LDLR...
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Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 fi 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf
Ngày tải lên :
14/02/2014, 22:20
... Kodama
1
1 Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Japan
2 Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry, ... degree of polymerization of 2–6 and methyl a-
D-gluco-
pyranoside as a glucose analog indicate that the activity increased with an
increase in the degree of polymerization. The production of insoluble ... measured as a
function of dextran concentration, the activity of
GSGB was highly dependent on dextran (filled circles
in Fig. 2B); however, that of GS was nearly zero, inde-
pendently of dextran concentration...
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Tài liệu Báo cáo khoa học: Influence of modulated structural dynamics on the kinetics of a-chymotrypsin catalysis Insights through chemical glycosylation, molecular dynamics and domain motion analysis pptx
Ngày tải lên :
19/02/2014, 05:20
... to
the study of the kinetics of catalysis by the serine pro-
tease a-CT we were able to statistically correlate the
structurally dynamic behavior of the enzyme with its
kinetics of catalysis. ... within a cutoff distance (r
c
¼ 6.0 A
˚
). The dynamics of
the resulting network are then defined by the Ni · Nj Kir-
chhoff connectivity matrix of interresidue contacts (G)
where the off diagonal ... and protonation rates of Ser195 thus reducing
the kinetics of catalysis. The results also suggest that
the dynamics of the calcium binding site in this struc-
tural class of proteins (chymotrypsin-fold...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
Ngày tải lên :
21/02/2014, 15:20
... GTP activation of the
glutaminase activity in the presence of 0.1 m
M
(open circles) or 1 m
M
(closedcircles)eachofUTPandATP-cS. (C) GTP activation of CTP
synthesis at 0.1 m
M
each of UTP and ATP.
4776 ... on
the basis of data (Table 2) from GTP activation of the glutaminase
activity in the presence of 0.1 m
M
each of UTP and ATP-cSandis
shown for comparison.
Ó FEBS 2002 Glutaminase activity of CTP synthase ... correlation of a decrease in
K
a
for GTP with the lowering of the concentration of
nucleotide substrates, has been reported previously [4].
Even though a full description of the mechanism of GTP
activation...
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Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx
Ngày tải lên :
22/02/2014, 08:20
... may affect the
estimation of Young’s modulus of biological fibers.
10
Further, for validation of our
computational model for biological protein materials consisting of protein crystals, as
shown ... illustration of biological protein materials composed of protein
crystals. (a) cartoon of a fiber, made of protein crystals, under mechanical loading. (b)
protein crystal lattices constituting the biological ... degree -of- fold, Q, is responsible for high yield stress of biological protein
materials through breakage of hydrogen bond of β-sheet structural motif.
For deeper understanding the role of native...
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Báo cáo khoa học: Mechanisms and kinetics of human arylamine N-acetyltransferase 1 inhibition by disulfiram potx
Ngày tải lên :
07/03/2014, 02:20
... the action of DS have also
begun to emerge with the identification of new pro-
tein targets of this drug [6–8]. Among them, XMEs
such as CYP 2E1 and certain GST isoforms have
Inhibition of NAT1 functions ... concentrations of the non-physiological reductant
dithiothreitol. These data suggest that the DS-depen-
dent inhibition of NAT1 is unlikely to be a result of
the formation of a mixed disulfide, but rather of ... of micromoles have been reported for differ-
ent GST isoforms [10]. Kinetic analysis has also shown
that the DS-dependent inhibition of NAT1 occurs
rapidly with a second-order rate constant of
6...
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Báo cáo khoa học: ATP-dependent ligases in trypanothione biosynthesis – kinetics of catalysis and inhibition by phosphinic acid pseudopeptides doc
Ngày tải lên :
07/03/2014, 04:20
... concentration of
phosphinate and initiating with enzyme.
Modality of inhibition
The mode of inhibition of the slow-binding phosphi-
nate was determined by examining the effect of varying
each ... disease-causing
parasites.
Results
Initial velocity analysis of the kinetic mechanism
of GspS
A matrix of kinetic data was collected in order to deter-
mine the kinetic mechanism of GspS. Six families of
kinetic data were generated ... three of four
residues involved in binding ATP; blue triangles, four of five residues interacting with GSH; yellow triangles, two of three residues implicated
in binding of the Spd moiety of the...
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Báo cáo khoa học: GTP binding and hydrolysis kinetics of human septin 2 pot
Ngày tải lên :
07/03/2014, 12:20
... K
d
of SEPT2 is 0.28 lm and that of
GDP is 1.7 lm in the presence of physiological Mg
2+
concentrations. These values are about an order of
magnitude lower than those observed for complexes
of ... mutagenesis of Ser218 to Ala,
as similar analysis of the mutated protein showed no
evidence of phosphorylation [26]. Residue S218 is
detected by a variety of phosphorylation prediction
software to ... completion of most of the studies described
herein. Given the conserved nature of this residue we there-
fore mutated the residue back to arginine and found that
none of the properties of the protein...
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Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elafin and pre-elafin (trappin-2) expressed in Pichia pastoris ppt
Ngày tải lên :
07/03/2014, 15:20
... system
of choice to produce large amounts of therapeutic proteins.
Fig. 4. Dissociation kinetics of HNE–rec-elafin complexes. (A) Time
course of p-nitroaniline release resulting from the hydrolysis of ... 1.1 m
M
[26].
Fig. 1. Evolution of rec-trappin-2 production by Pichia pastoris as a
function of the duration of fermentation. Aliquots of concentrated
supernatants of rec-trappin-2-secreting P. ... (2001) Kinetics of the Inhibition of
Proteinase 3 by Elafin. Am. J. Respir. Cell Mol. Biol. 24, 83–89.
25. Bieth, J.G. (1995) Theoretical and practical aspects of proteinase
inhibition kinetics. ...
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Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx
Ngày tải lên :
08/03/2014, 08:20
... concentra-
tion [N] and the results of the inhibition studies show that,
despite of the high rate of hydrolysis of the acyl-enzyme,
tight binding of the nucleophile and displacement of the
catalytic H
2
O ... 7).
The V
s
/V
h
ratio is the ratio of the reaction rates of
aminolysis and hydrolysis of the acyl-enzyme. The curva-
ture of the plot of V
s
/V
h
vs. the mole fraction of D
2
O
indicates therefore ... rate of deacylation. The theoretical maxi-
mum of the amplitude of the burst phase is equal to the total
enzyme concentration. When k
h1
¼ 5 k
2
, only 2% of the
maximum of the amplitude of the...
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Báo cáo khoa học: Kinetics of violaxanthin de-epoxidation by violaxanthin de-epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers pptx
Ngày tải lên :
08/03/2014, 10:20
... to
analyse the kinetics of conversion of violaxanthin to
zeaxanthin. The model allowed us to follow independently
the kinetics of the two de-epoxidation steps: the conver-
sion of violaxanthin ... proportion of monogalactosyldi-
acylglycerol; at 30 mol% of this lipid, their values increase
43 and 76 times, respectively, while values of corresponding
parameters describing kinetics of antheraxathin ... is
thetimeofreaction(Dt ¼ a constant time interval,
n ¼ number of time intervals).
Measurement of the order parameter in liposome
membrane
Temperature dependent changes of the order parameter of
lipid...
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Patenting of Biological Material and Biotechnology
Ngày tải lên :
13/03/2014, 21:59
... Any variety of animal or plant of any essentially
biological process for the production of animals
or plant, not being a micro -biological or other
technical process or the product of such a ... synergistic union of the
biological sciences and technology based industrial
art. It is the utilization of biological processes for the
exploitation and manipulation of living organisms or
biological ...
Lucknow, 2002
19 Directive 98/44/EC of the European Parliament and of the
Council of 6 July 1998 on the legal protection of
biotechnological inventions, 1998 Official J. Eur.
Communities O.J....
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Báo cáo khoa học: Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin pdf
Ngày tải lên :
16/03/2014, 11:20
... presence of an excess of
NADH, two events can be deconvoluted: the reduction
of [Fe-Cys
4
] (monitored at 560 nm) and the formation
of semiquinone FMN (monitored at 390 nm after sub-
traction of the ... s
)1
in Scheme 1.
Reduction of FlRd by flavorubredoxin reductase
Spectral analysis of FlRd is complex due to the partial
overlap of the optical contribution of its redox cofac-
tors. Figure 4 shows ... spectrum of FlRd
in the oxidized state (spectrum A) and after reduction
by an excess of NADH in the presence of catalytic
amounts of FlRd-reductase (spectrum B). In the visible
region, the spectrum of...
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Growth kinetics of silicon nanowires by platinum assisted vapour–liquid–solid mechanism
Ngày tải lên :
16/03/2014, 15:05
... is
dependent on the type of catalyst. This could be explained by
the effect of the catalyst on the barrier energy to the nucleation
of a new step at the growth front of the nanowires (i.e., the ... incorporation of the Si atoms in a
crystal lattice occurs). The (11 1) plane of Si is a smooth surface
where the formation of a new lattice plane is difficult [17] and,
thus the incorporation of Si atoms ... rate of incorporation of Si from the liquid
phase to the lattice.
The present results suggest that Pt is a good candidate for the
VLS growth of Si nanowires. However, the growth temperature of
the...
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- free nucleotides in living cells play important roles in a variety of biological reactions
- department of biological sciences
- genetic basis of biological sexuality
- adaptive recurrent neural network control of biological wastewater treatment
- department of biological sciences lsu
- department of biological sciences columbia university
- department of biological sciences notre dame
- department of biological sciences fiu
- department of biological sciences ncsu
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