isolation purification and quantification of eps
Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
Ngày tải lên :
11/09/2015, 10:06
... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... 79 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration Methods ... saponin identification and quantification The purification process is complex and laborious with long sample preparation and extraction times and yielding relatively low amounts of soyasaponins from...
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Purification and cloning of PCR products
Ngày tải lên :
25/10/2013, 22:20
... ethidium bromide and UV irradiation for detection of DNA in gels, thereby preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors ... ordering process and the cost of synthesis is relatively cheap An example of appropriate design of primers to add attB1 and B2 sites to the 5′ and 3′ ends respectively of a gene of interest are ... N-glycosidic bonds between uracil and deoxyribose leading to a loss of the base The loss of bases leads to disruption of base pairing and therefore exposure of single strands that can be annealed to...
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Atmospheric volatile organic compound measurements during the Pittsburgh Air Quality Study: Results, interpretation, and quantification of primary and secondary contributions pot
Ngày tải lên :
05/03/2014, 21:20
... the roles of primary and secondary OC production is an important step toward an improved understanding and modeling of the sources, morphology and effects of aerosol OC The technique of minimizing ... concentrations, creating an understanding of the variety of sources contributing to a broad range of measured species [Sweet and Vermette, 1992; Thunis and Cuvelier, 2000; Lamanna and Goldstein, 1999] In ... importance of different types of VOCs to regional photochemistry, and the relationship between aerosol concentrations and the chemical state of the atmosphere; and quantifying the relative importance of...
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Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot
Ngày tải lên :
20/06/2014, 23:20
... thankful to Professor Les Copeland (Faculty of Agriculture, Food and Natural Resources - The University of Sydney, New South Wales, Australia) and Professor Kaeko Kamei (Department of Biomolecular ... proteins Generally, the two-step chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles represented pure proteins Fraction ... alignment of fragments obtained from MS/MS analyses of FIII-1 and FIII-2 from P excavatus with lumbrokinase and its precursor from L rubellus (sp:P83298 and U25647) and E fetida (gpu:EU167737 and AY438624),...
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Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc
Ngày tải lên :
07/08/2014, 20:23
... seniccav ,oga sraey wef A ]81,2[ secnamrofrep evitcudorper dna gnideef rieht no tceffe suoireteled a gnicudorp ,kcit eht nihtiw )s(negitna tegrat a rof cificeps seidobitna tsegni thgim stsoh dezinummi ... ,BKL( rettolb yrd-imes tolBavoN a gnisu demrofrep saw sisylana tolbonummI la te gnittolbonummI ]31[ ilmmeaL yb debircsed snoitidnoc eht ot gnidrocca demrofrep saw siserohportcele dna ,selpmas ,sleg ... noitaraperP )ASU ,daRoiB( lleC balS-lauD II naetorP -iniM eht ni demrofrep saw sisylana citerohportcelE EGAP-SDS ]3[ dohtem drofdarB eht yb detamitse saw sPLG eht fo tnetnoc nietorp ehT fo sPLG...
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Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps
Ngày tải lên :
07/08/2014, 23:22
... Detection limit and standard curve of the multiplex real-time PCR The detection limit and standard curve of the multiplex real-time PCR was determined using S Typhimurium ATCC 14028 and S Enteritidis ... the results of the standard culture method of isolated field and reference strains with those of the multiplex real-time PCR Results Specificity of the multiplex real-time PCR A total of 128 bacterial ... 3.57 ± 0.02 and 3.57 ± 0.03 log10 CFU/ml for S Typhimurium and S Enteritidis in beef, and 4.57 ± 0.02 and 2.26 ± 0.05 log10 CFU/ml for S Typhimurium and S Enteritidis in pork (Figs and 4) Our...
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Báo cáo y học: "Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus" pps
Ngày tải lên :
12/08/2014, 01:21
... Journal 2010, 7:241 http://www.virologyj.com/content/7/1/241 Page of Figure Expression and purification of the tgK protein M represented standard protein molecular weight markers The arrow marked the ... http://www.virologyj.com/content/7/1/241 Page of Figure Purification of the rabbit anti-tgK polyclonal antibody and Western blot assay M represented standard protein molecular weight markers; M1 ... http://www.virologyj.com/content/7/1/241 Page of Figure Indirect immunofluorescent microscopy was used to monitor the DEV antigen distribution in liver, harder’s glands, cecum, spleen and kidney of the infected ducks...
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Báo cáo khoa học: " Detection and quantification of pestivirus in experimentally infected pregnant ewes and their progeny" pps
Ngày tải lên :
12/08/2014, 04:20
... consisting of 22.5 μl of BehiBVD/BD-VK Master Mix and 2.5 μl of RNA, using the following program: at 42°C, 10 s at 95°C and 40 cycles of s at 95°C and 34 s at 60°C Detection capacity of the kit ... (BDV-genotype 4) along with their progeny and a summary of qualitative and quantitative results of qRT-PCR in placentae, and blood and tissues from the offspring Groupa Lambingb (Ewe ID) PLACENTAE ... per ml of Box Box Plot representing log2 viral genome copies per ml of blood in the offspring grouped according to the time of infection Group A, offspring of ewes challenged at day 108 of gestation;...
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Báo cáo khoa học: " Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection" pps
Ngày tải lên :
12/08/2014, 04:21
... identified in the regions of E1, nsP1, nsP2, Page of and nsP4 genes More than 20 sets of primer pairs were synthesized and screened for initial evaluation of their specificity and sensitivity (Data ... (GenBank accession no: AF490259 of Ross reference strain, EU703762.1 of Malaysia strain, EF027140.1 of Indian strain, AM258995.1 of Reunion strain and EF452494.1 of USA strain), the potential target ... Singapore Page of Authors’ contributions JJHC and PSH designed research; PSH and JJHC performed research; PSH, MLN and JJHC analyzed data and wrote the paper All authors have read and approved...
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Báo cáo khoa học: "Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate" doc
Ngày tải lên :
12/08/2014, 04:21
... diagnostics and epidemiologic surveys Methods Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification ... Alignment of the amino acid sequence of coat protein of different LSV isolates and other carlavirus Figure Alignment of the amino acid sequence of coat protein of different LSV isolates and other ... Laboratory of Arid and Grassland Agroecology of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou 730000, China and 4Department of Bacteriology, Graduate School of Medical Science,...
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investigation and quantification of codon usage bias trends in prokaryotes
Ngày tải lên :
30/10/2014, 20:09
... Ph.D Dean, School of Graduate Studies ABSTRACT Hanes, Amanda L M.S., Department of Computer Science and Engineering, Wright State University, 2009 Investigation and quantification of codon usage ... strength, and also suggests that codon usage bias in general follows the trend of being either strong and global to the genome, or weak and present in only a subset of the genome Analysis of these ... molecular level than that of amino acids and proteins Further investigation of this evolutionary mechanism will provide a greater understanding of its effects on different types of organisms, enabling...
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Carbon nanotubes and branched DNA based nucleic acid assays towards a PCR free detection and quantification of nucleic acids
Ngày tải lên :
12/09/2015, 09:28
... Results and Discussion 137 4.3.1 Principle of the preparation of p185-ssDNA standard 137 4.3.2 Gel electrophoresis 140 4.3.3 Quantification of mRNA 141 4.3.4 Quantification ... scientific user facility sponsored by the U.S Department of Energy's (DOE's) Office of Biological and Environmental Research and the core R&D Laboratory of Fungal Biotechnology funded by the DOE Biomass ... Synthesis of the biotinylated double-strand DNA (dsDNA) .131 4.2.6.2 Purification of biotinylated dsDNA and gel electrophoresis .132 4.2.6.3 Purification of sense p185-ssDNA 133 4.2.7 Real-time...
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Assessment and quantification of foetal electrocardiography and heart rate variability of normal foetuses from early to late gestational periods 5
Ngày tải lên :
15/09/2015, 17:10
... development of the sympathetic and parasympathetic nervous systems This study shows the use of spectral analysis of foetal HRV as an indirect indication Limitations of the study and future directions ... directions 205 of the development of foetal cardiac autonomic activity from 18 weeks of gestation to term Notably this is a longitudinal study of a cohort of healthy foetuses The comparison of HRV results ... valuable information to the field of non- invasive foetal ECG and to the understanding of the development of the foetal autonomic nervous system via measurement of heart rate variability, it has...
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Assessment and quantification of foetal electrocardiography and heart rate variability of normal foetuses from early to late gestational periods 4
Ngày tải lên :
15/09/2015, 17:11
... standard deviation of the differences (indicated by the spreading out of Comparison of novel versus commercial HRV softwares 188 Table 11-2: Bland-Altman analysis (mean difference) of time- and ... difference and the mean, and whether the assumption of constant standard deviation of the differences is satisfied When the data points on the Bland-Altman plot spread out as the magnitude of measurements ... Nevokard and F-EXTRACT, respectively Comparison of novel versus commercial HRV softwares 191 data points) with increasing values of SDNN, rMSSD and pNN27 As recommended, Bland-Altman plots of percent...
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Assessment and quantification of foetal electrocardiography and heart rate variability of normal foetuses from early to late gestational periods 3
Ngày tải lên :
15/09/2015, 17:11
... normal and ectopic beats and thus gave an accurate measure of the true fHR The case history highlights the usefulness of non-invasive fECG in the determination of foetal heart rate and ECG and its ... DEVELOPMENT OF A NOVEL HRV SOFTWARE Development of a novel HRV software 141 Introduction In collaboration with the Computer Science Department of National University of Singapore, a software (F-EXTRACT) ... foetal heart rate mNN msec Mean of all NN intervals SDNN msec Standard deviation of all NN intervals rMSSD msec The square root of the mean of the sum of the squares of differences between adjacent...
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Assessment and quantification of foetal electrocardiography and heart rate variability of normal foetuses from early to late gestational periods 2
Ngày tải lên :
15/09/2015, 17:11
... deviation of NN interval and is defined as: where NNi is the duration of the i-th NN interval, n is the number of all NN intervals and m is their mean duration Since variance (square of standard ... traces, and (c) averaged foetal ECG complex recorded by FEMO M= maternal QRS complex; F= foetal QRS complex; Subscripts o and t = onset and termination points of P, QRS and T waves 93 Materials and ... signals; F’ and F’’- Foetal ECG signals after subtraction of M from MF’ and MF’’ Materials and methods 89 Figure 6-5a: Comparison of foetal heart rate recorded by direct (scalp) and abdominal...
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Assessment and quantification of foetal electrocardiography and heart rate variability of normal foetuses from early to late gestational periodsb 1
Ngày tải lên :
15/09/2015, 17:11
... index Mean of the standard deviation of RR intervals for each 5-minute segment of the entire recording SDANN Standard deviation of the mean of RR intervals for each 5-minute segment of the entire ... system and those of a commercial system, and demonstrated Summary xii the importance of the processing of artifact beats in foetal RR-intervals for accurate foetal HRV determination List of Tables ... interval begins at the onset of the QRS complex and to the end of the T wave (Qon to Ttm) It represents the time between the start of ventricular depolarization and the end of ventricular repolarization...
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purification and characterizations of phytase enzyme form aspergillus niger
Ngày tải lên :
06/10/2015, 13:05
... hexakisphosphate) is the primary storage form of phosphorus in plants They occupy about 1-3% of the seeds of grains and legumes, and 60-80% of the total amount of plant phosphorus (Nelson, 1967) Phosphorus ... PuriWcation and structural characterization Protein Expression and Purification 46, pp.221–232 Bradford, M.M 1976 A rapid and sensitive method for quantitation of microgram quantities of protein ... determination of inorganic orthophosphate and it’s application to the assay of inorganic pyrophosphate Anal Biochem 113:313–317 Kim, Y O., Kim, H K., Bae, K S., Yu, J H and Oh, T K 1998 Purification and...
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Isolation, cloning and characterization of the FSH beta gene promoter of the chinook salmon (oncorhynchus tshawytscha)
Ngày tải lên :
08/11/2015, 17:15
... surface of the pituitary gonadotrope This leads to the synthesis and secretion of LH and FSH which stimulate the production of steroid hormones in their gonads Testosterone (T), estrogen (E) and ... stimulatory effects of GnRH and activin during puberty and sexual maturation 1.5 Aim The aim of this project is to initiate research on the mechanism of transcriptional regulation of the FSHβ subunit ... responsiveness of the promoter and the sequence will be analyzed to identify putative regulatory elements that may mediate these effects 23 MATERIALS AND METHODS 2.1 Extraction and isolation of genomic...
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Isolation, cloning and expression of NAD+ glycohydrolase from neurospora crassa
Ngày tải lên :
08/11/2015, 17:15
... Culture 2.2.2 Conidia Culture 32 33 ii CONTENTS 2.3 PAGE Isolation of N crassa Proteins 2.3.1 Isolation of Mycelia Proteins 2.3.2 Isolation of Conidia Proteins 33 33 Column Chromatography 2.4.1 ... Cycling Assay 81 Discussions 4.1 Isolation and Characterization of Conidial NADase from N crassa 83 4.2 Identification of NADase Sequence 85 4.3 Cloning and Expression of Gi|85106032 86 4.4 NAD+ Cycling ... LIST OF FIGURES FIGURE TITLE PAGE 1.1 De novo synthesis and salvage pathway of NAD+ 1.2 Reaction mechanism of NADase and ADP-ribosyl cyclase 1.3 Cycling assay for cADPR 15 1.4 Asexual cycle of...
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