isolation pcr amplification and sequencing

Báo cáo y học: " Tenofovir treatment augments anti-viral immunity against drug-resistant SIV challenge in chronically infected rhesus macaques" pot

Ngày tải lên : 13/08/2014, 09:20

... discriminate between the two viruses, 2) PCR to detect wild-type and nef-deleted alleles, and 3) PCR amplification and direct sequencing of regions of the SIV pol gene to identify drug resistance ... was undetectable and remained so throughout a year of follow-up Several blips of viremia occurred, and analyses of both viral RNA and DNA demonstrated persistence of nef deletions and pol sequences ... SIV-specific antibodies and CD8+ T cell responses were evaluated in both tenofovir-treated and control macaques before and after challenge with SIVmac055 Antibodies to both SIV gp130 and p27 antigens...

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Báo cáo y học: "High-Resolution Flow Cytometry: a Suitable Tool for Monitoring Aneuploid Prostate Cancer Cells after TMZ and TMZ-BioShuttle Treatment"

Ngày tải lên : 26/10/2012, 09:48

... Mickey GH, and Paulson DF Isolation of a human prostate carcinoma cell line (DU 145) Int J Cancer 1978; 21: 274-281 Ehemann V, Hashemi B, Lange A, and Otto HF Flow cytometric DNA analysis and chromosomal ... 8: 1153-1165 50 Shimada M and Nakanishi M DNA damage checkpoints and cancer J Mol Histol 2006; 37: 253-260 51 Damia G and Broggini M Cell cycle checkpoint proteins and cellular response to treatment ... the development of normal and neoplastic cells Adv Cancer Res 1991; 57: 1-46 61 Jenkins RB, Qian J, Lieber MM, and Bostwick DG Detection of c-myc oncogene amplification and chromosomal anomalies...

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báo cáo hóa học:" Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor antagonist" potx

Ngày tải lên : 18/06/2014, 15:20

... Software House) Curve fitting and determination of EC50 and IC50 values was performed using Prism 4.0 (Graphpad) when applicable The mean, standard deviation, standard error and % coefficient of variation ... on Day 1, and again on day (9 individuals only), 8, 15, 29, 43, 57, 71, 85, and 113 All 108 (54 placebo and 54 dosed) individuals were assessed at all time points except day Mean, standard deviation ... column (10 × 250 mm) and BioCad Vision Workstation Labeled Peaks were identified and examined for their ability to bind to receptor positive cells Identified peaks were pooled and retested for binding...

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báo cáo hóa học:" Static platelet adhesion, flow cytometry and serum TXB2 levels for monitoring platelet inhibiting treatment with ASA and clopidogrel in coronary artery disease: a randomised cross-over study" potx

Ngày tải lên : 18/06/2014, 15:20

... insets are the comparisons between the control means of visit and and treatment with ASA (A), clopidogrel (C) and the combination of ASA and clopidogrel (A+C) The respective figures show the effect ... (ASA and clopidogrel alone as well as ASA and clopidogrel combined) Discussion With the aim of finding variables sensitive to clopidogrel and ASA-treatment, this study used a screening approach and ... platelet adhesion and serum TXB2-levels and performed the statistical analyses All authors participated in the design of the study, cooperated in the drafting of the manuscript and read and approved...

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Báo cáo khoa học: " Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules" ppsx

Ngày tải lên : 07/08/2014, 20:23

... RTH2A and RTH230A; RT21A and RTH21A; and RTH26A, RTH65A, and Ken5; recognize Pan T1, Pan T2, and Pan T4 LDM respectively Zenon second step antibodies were used to demonstrate RTH2A (IgG1) and RTH230A ... CD8 CD8 CD4 and CD8 subpopulations Basophils and subpopulation of CD4+ T lymphocytes Granulocytes, basophils, and monocytes Subpopulations of B and T lymphocytes Subpopulations of B and T lymphocytes ... (198) and subsequent Fig Two color analysis of the expression of LDM detected with RACT20A and MRB120A on basophils, monocytes, and CD4 T lymphocytes mAbs to CD14 and Pan T1 were combined and used...

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Báo cáo y học: "Flow cytometry analysis of glucocorticoid receptor expression and binding in steroid-sensitive and steroid-resistant patients with systemic lupus erythematosus" pps

Ngày tải lên : 09/08/2014, 14:22

... patients and controls Disease activity scores were determined before and after steroid treatment (Table 1) and the SLE patients were divided into the SR and the SS groups accordingly GR expression and ... collected and stored at -80°C for measurement of IL-12, TNF-α and IFN-γ levels by ELISA (ebioscience, San Diego, CA, USA) The minimum limits of detection for IFN-γ, IL-12 p40 and TNF-α were 8, 80 and ... GR expression and binding (mean ± standard error of the mean) For heterogeneity of variance, Kruskal-Wallis H test and Memenyi test were used Age, gender, dosage of prednisolone and years since...

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Báo cáo y học: "The basophil activation test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives" pot

Ngày tải lên : 13/08/2014, 13:22

... LM, Metcalfe DD, Prussin C: Frequency and characterization of antigen-specific IL-4 and IL13 producing basophils and Tcells in peripheral blood of healthy and asmathic subjects J Allergy Clin ... histamine release (and as a consequence the CD63 expression) and the upregulation of CD203c The rise in CD63 or CD203c expression (measured by fluorescence / Phycoerythrin) before and after allergen ... test and/ or specific IgE ≥ class III Healthy donors, from our laboratory, were not known to be allergic and presented total IgE < 100 kU/L In terms of clinical interpretation, sensitivity and...

8
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Báo cáo y học: "Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts" pps

Ngày tải lên : 14/08/2014, 17:22

... morphologic parameters and two fluorescence intensities The morphologic parameters are forward light scatter (FSC) and sideward light scatter (SSC), and they measure cell size and cell granularity ... corresponds to cell size and SSC to cell granularity Several subpopulations can be distinguished: (I) healthy and well measured cells, (II) cell debris, and (III) cell conjugates and air bubbles (b) ... Good software for this is available in the R packages qvalue and multtest, and we recommend the reports by Storey [28] and Pollard [29] and their coworkers for methodologic background Even after...

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424
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Intake manifold design effect on air fuel mixing and flow for an LPG heavy duty engine

Ngày tải lên : 05/09/2013, 16:30

... engine performance The air-fuel ratio and the specific fuel consumption are measured and determined With the optimized manifold, both AFR and SFC are improved by % and 28 % respectively ISSN 2076-2895 ... 2010 [11] Fuchs T R and C J Rutland Intake Flow Effects on Combustion and Emissions in a Diesel Engine, SAE N° 980508, 1998 [12] Ugur Kesgin, Study on the design of inlet and exhaust system of ... supercharging and filling Lee [8] has developed a computer program to predict the engine performance characteristics through the analysis of the flow in the intake and exhaust systems and of the...

12
891
1

Clinical Flow Cytometry – Emerging Applications Edited by Ingrid Schmid ppt

Ngày tải lên : 08/03/2014, 19:20

... permeabilized and stained with mAbs directed against IL-1 and CCR5, IL-2 and CCR5, IFN and CCR5, IL-4 and CCR4, or IL-10 and CCR4 and were analyzed on a flow cytometer A, B, C, D, and E FACScan ... Candida to fluconazole and echinocandin, and compare results with the standard methods (MIC determined by macrodilution and/ or Etest according to National Committee for Clinical Laboratory Standard, ... cells and non nucleated debris) and tested monoclonal antibodies which are directed at both immediate early/early genes (IE62) and late, structural proteins (VZV gE, gI, gB, and gH and MCP) and...

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flow cytometry protocols 2d ed - teresa s. hawley

Ngày tải lên : 08/04/2014, 12:51

... 27–44 47 Pinkel, D and Stovel, R (1985) Flow chambers and sample handling, in Flow Cytometry: Instrumentation and Data Analysis (Van Dilla, M A., Dean, P N., Laerum, O D., and Melamed, M R., ... used in cytometry Uptake and efflux of dyes, drugs, and other reagents by and from bacteria are affected by the structure of the cell wall, and by the presence of pores and pumps that may or may ... by noise and debris 40 Shapiro and Nebe-von-Caron DiOC2(3) is excited at 488 nm; its green fluorescence is detected through a 525to 530-nm bandpass filter with approx 20 nm bandwidth and its...

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flow cytometry protocols

Ngày tải lên : 11/04/2014, 09:41

... scattering and fluorescence 1scollected, filtered, and converted into electrical signals by the optical and electronics system The data storage and computer control system saves acquired data and 1s ... , Heusser, C., Neumann, C., and Rieger, C (1993) Detection of intracellular cytokmes by flow cytometry J Immunol Meth 159, 197-207 Sander, B., Andersson, J., and Andersson, U (1991) Assessment ... immunofluorescence and the paraformaldehyde-saponin procedure Immunol Rev 119,65-93 Sander, B., Hoiden, I., Andersson, U., Moller, E., and Abrams, J S (1993) &mllar frequency and kinetics of cytokine...

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hughes t. groove and flow - six analytical essays on the music of stevie wonder (диссертация)

Ngày tải lên : 29/05/2014, 21:49

... of rhythm and blues, soul, and funk in general, and from Wonder’s music in particular, there is a strong and conspicuous relationship between them, both in terms of cultural history and musical ... both major and minor thirds At a more basic level, vocalists and 30 The result is three precisely synchronized cycles: As the right hand moves up and away from the register of the left hand, it ... CUNY, and Christopher Waterman of UCLA for assistance, advice, and inspiration The members of the Stevie Wonder Internet Discussion List were indispensable as a font of knowledge, understanding, and...

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Báo cáo hóa học: "Exhaustive expansion: A novel technique for analyzing complex data generated by higherorder polychromatic flow cytometry experiments" pot

Ngày tải lên : 18/06/2014, 16:20

... and AR performed the research JCS and WM contributed vital analytical tools JCS, LW, AR, BZ, and EBW analyzed and interpreted the data JCS and EBW wrote the manuscript All authors have read and ... LTM,+++.-,1.81 The Expander program derives aggregate sets or supersets from input data, and outputs both the relative set name and the percentage of cells in both the newly derived sets and the original ... primitive pluripotent stem cells (ckit, CXCR4, and Sca-1), differentiated myoblasts (CD56 and CXCR4), and vascular-relative cells (CD146, CD31, CD144, CD105, and VEGFR2) were used to more comprehensively...

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476
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CLINICAL FLOW CYTOMETRY – EMERGING APPLICATIONS pot

Ngày tải lên : 28/06/2014, 15:20

... permeabilized and stained with mAbs directed against IL-1 and CCR5, IL-2 and CCR5, IFN and CCR5, IL-4 and CCR4, or IL-10 and CCR4 and were analyzed on a flow cytometer A, B, C, D, and E FACScan ... Candida to fluconazole and echinocandin, and compare results with the standard methods (MIC determined by macrodilution and/ or Etest according to National Committee for Clinical Laboratory Standard, ... cells and non nucleated debris) and tested monoclonal antibodies which are directed at both immediate early/early genes (IE62) and late, structural proteins (VZV gE, gI, gB, and gH and MCP) and...

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Báo cáo y học: " Diagnostic value of anti-human citrullinated fibrinogen ELISA and comparison with four other anti-citrullinated protein assays" potx

Ngày tải lên : 09/08/2014, 08:22

... defined for anti-pepA and anti-pepB antibodies corresponds with a specificity of 100% and 99.3% and a sensitivity of 63.6% and 54.2%, respectively [14] Detection of anti-CCP1 and anti-CCP2 antibodies ... performed with the results of RF and five ACPA assays: anti-CCP1, anti-CCP2, anti-pepA, anti-pepB and AhFibA (Figure 3) Table Sensitivities and specificities of AhFibA and anti-CCP2 assay in the two ... value of the AhFibA ELISA and the anti-CCP2 ELISA, and conclude that both assays perform equally well, which is reflected by similar ROC curves and similar sensitivities and specificities There...

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625
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Báo cáo khoa học: "Cutaneous vascular reactivity and flow motion response to vasopressin in advanced vasodilatory shock and severe postoperative multiple organ dysfunction syndrome" pot

Ngày tải lên : 12/08/2014, 23:22

... transport and consumption variables, norepinephrine and milrinone requirements, as well as pH and arterial lactate concentrations were documented immediately before and hour after randomization ... mechanically ventilated and received analgesic and sedative drugs (for instance, continuous infusion of sufentanil and midazolam) There was no difference in the dosage of analgesic and sedative drugs ... participated in its design and coordination, carried out bedside measurements and documentation, and drafted the manuscript MWD conceived the study protocol, participated in its design and coordination,...

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Báo cáo y học: "Differential response of human basophil activation markers: a multi-parameter flow cytometry approach" pot

Ngày tải lên : 13/08/2014, 13:22

... Authors' contributions SC and PB conceived the study, performed the experiments and wrote the manuscript, AV and RO assisted in the flow cytometry setting and handling, MDG and PS participated in ... providing enriched and functional leukocyte samples, the overall procedure of basophil isolation and pooling has the advantages of washing out the plasma and the majority of platelets and of reducing ... and histograms of CD63 and CD203c expression in response to fMLP Dot plots and histograms of CD63 and CD203c expression in response to fMLP Basophils were incubated in the absence (A,B,E,G) and...

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Báo cáo y học: " Assessment of monocytic HLA-DR expression in ICU patients: analytical issues for multicentric flow cytometry studie" pptx

Ngày tải lên : 13/08/2014, 21:21

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Influence of pipe length and flow rate on nano particle deposition in laminar circular pipe flows

Ngày tải lên : 09/09/2015, 10:06

... different lengths of 1, and cm and a mean velocity of m/s Figures 8-10 display the deposition efficiency for a pipe with the length of 5cm and different mean velocity of 0.5, and m/s in compare with ... the particle and D is the diffusion coefficient given as [8]: ~ k TC D= B c 3πµd p (6) Geometry and mesh structure The geometry of a straight pipe is created in Gambit software and used in this ... with the length of 1cm and a mean velocity of m/s Figure Comparison of the deposition efficiency for the 1cm long pipe and a mean velocity of 1m/s between the present result and the analytical expressions...

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