isolation and functional assessment of mitochondria from small amounts of mouse brain tissue
báo cáo khoa học: " Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset" pptx
Ngày tải lên :
12/08/2014, 03:21
... peach and Arabidopsis Figure selected accuracy of the predicted expression patterns of Evaluation of the genes by RT-PCR Evaluation of the accuracy of the predicted expression patterns of selected ... promoters containing these types of cis-elements and orthologous transcription factors However, the identification and functional characterization of these types of promoters in fruit trees is ... this injury A better understanding of cold acclimation and coldresponsive genes in peach trees and fruits may provide clues about the association of cold acclimation and chilling injury Several...
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báo cáo khoa học: " Isolation and functional characterization of Lycopene β-cyclase (CYC-B) promoter from Solanum habrochaites" ppt
Ngày tải lên :
12/08/2014, 03:21
... expression, and thus can be a valuable tool in understanding carotenoid metabolism in tomato Methods Isolation and cloning of ShCYC-B promoter Isolation of 5' flanking region of CYC-B gene from S ... Doyle JL: Isolation of plant DNA from fresh tissue Focus 1990, 12:13-15 Orzaez D, Mirabel S, Wieland WH, Granell A: Agroinjection of tomato fruits A tool for rapid functional analysis of transgenes ... levels of lycopene β-cyclase (CYC-B) in different tissues of S lycopersicum and S habrochaites RT-PCR was performed with total RNA isolated from leaves, flowers and fruits at different stages of...
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Báo cáo Y học: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases pot
Ngày tải lên :
08/03/2014, 23:20
... linalool and a-terpineol cDNA isolation and sequencing Random sequencing of a cDNA library made from mRNA isolated from the peel of young lemon fruits resulted in the identification of nine putative ... (featuring a Km of 3.1 lM and an apparent Vmax of 28.49 lmolÆh)1Æmg)1) and a substrate inhibition curve (featuring a Km of 13.5 lM, an apparent Vmax of 89.47 lmolÆh)1Æmg)1 and a Ksi of 5.65 lM) were ... Ramachandiran, V., Katoh, S & Croteau, R (1999) cDNA cloning, characterization, and functional expression of four new monoterpene synthase members of the Tpsd gene family from grand fir (Abies grandis)...
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báo cáo khoa học: " Isolation and functional characterization of CE1 binding proteins" pptx
Ngày tải lên :
11/08/2014, 11:21
... Ler, Landsberg erecta Page 12 of 13 Additional file 3: Growth of AtERF13, RAP2.4 and RAP2.4L knockout and RNAi lines RNAi lines of AtERF13 and knockout lines of RAP2.4 and RAP2.4L were prepared as ... #25 and #31 denote RNAi lines (B) Single or double knockout (KO) lines of RAP2.4 and RAP2.4L Top left, expression levels of RAP2.4 and RAP2.4L in the single knockout lines of RAP2.4 (RK) and ... the other hand, the core sequence of the CE1 element is CCACC, which differs from the DRE and the GCC box core sequences The results of our one-hybrid screen indicate that a subset of AP2/ERF...
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báo cáo khoa học: " Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L" potx
Ngày tải lên :
12/08/2014, 05:20
... Example 5of comparison between absorption spectrum of the reaction product and authentic standard Example of comparison between absorption spectrum of the reaction product and authentic standard ... Switzerland) with ART3For and ART3Rev primers (Table 1) designed from the C cardunculus HCT sequence: probe from globe artichoke, probe from cultivated cardoon, and probe from wild cardoon Page 12 of ... assay and the determination of kinetic parameters CGA was purchased from Sigma-Aldrich (Germany), and quinic acid from Fluka (Switzerland) CoA esters (substrates) were kindly provided by Dr Legrand...
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Báo cáo y học: "An evolutionary and functional assessment of regulatory network motifs" pps
Ngày tải lên :
14/08/2014, 14:21
... instances of the motifs; all the interaction patterns, irrespective of their abundance; and patterns composed of genes taken at random There are 481 instances of motifs in a total number of 9,962 ... the randomized ensemble The probabilities are estimated from a MonteCarlo sampling of 10,000 trials of the randomized ensemble distribution and the results are sensitive neither to the number of ... coherence of the transcription and the proteinprotein interaction datasets used for finding the motifs and the published annotations deposited research Types of motifs of order n = and n = for...
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Báo cáo y học: "Expression and functional properties of antibodies to tissue inhibitors of metalloproteinases (TIMPs) in rheumatoid arthritis" docx
Ngày tải lên :
09/08/2014, 06:23
... activation Several functional properties of TIMPs are of potential importance in the pathogenesis of arthritis Overexpression of TIMP-1 and TIMP-2 is associated with inhibition of apoptosis and invasive ... laboratory, and statistical evaluation of material from RA patients; and preparation of the manuscript LD performed the collection and clinical analysis of patients with degenerative joint diseases and ... patients with RA Samples of blood and synovial fluid from 89 patients with RA were tested for the presence of autoantibodies against all four types of TIMP (TIMP-1, -2, -3, and -4) and compared with...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
Ngày tải lên :
18/02/2014, 08:20
... positions of the Ca of Ala156 in HYDJs and the Ca of Met156 in HYDBp located in the SGL3, which leads to the increased size of the substrate binding pocket of HYDJs The side chain of Ala156 is much smaller ... performed using T-COFFEE [44] Docking simulation of substrate on the active site of HYDJs The substrate binding pockets of HYDJs and the mode of interaction between substrate and enzyme were analyzed ... 0.2 characterization and Jannaschia sp CCS1 evaluation of HYDJs from Characterization of HYDJs To explore the possible cause of the higher specific activity for conversion of d,l-p-HPH to N-carbamoylp-hydroxyphenylglycine...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
Ngày tải lên :
18/02/2014, 18:20
... plausibly involved in DNA recognition and binding and is positioned left of the active site [14] Rep245 is both capable of de novo synthesis of DNA primers and of elongating them Long DNA extension ... concentration of Rep245 prepared in 10 mm sodium phosphate (pH 7.4) was lm CD spectra were recorded at 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal ... nature of the acid residues within the active site of Rep245 The carboxylate triad of Rep245 including the D101, D103 and D166 motif is similar to the triads of X family DNA polymerases and terminal...
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Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf
Ngày tải lên :
21/02/2014, 00:20
... describes the cloning and functional characterization of an AK gene from a genomic library of L donovani as well as its expression and molecular characterization Materials and methods Materials ... after induction of the culture with IPTG, which matched the molecular mass predicted from the amino acid sequence of AK2 protein The purification and functional folding of L donovani AK from the E ... growth curve of promastigotes Functional characterization of recombinant AK The recombinant protein purified from the inclusion bodies and refolded in the presence of dithiothreitol, MgSO4 and KCl...
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Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt
Ngày tải lên :
21/02/2014, 03:20
... described the isolation of full-length cDNAs encoding three subtypes of the Xenopus laevis brain TRHR (xTRHR1, xTRHR2 and xTRHR3) and their functional expression in Xenopus oocytes and mammalian ... value and n represents the number of oocytes tested for each condition TRHRs and its similarity to most of the regions of the mouse, rat and fish TRHR2 indicate that xTRHR2 is a member of the ... isolated from the brain, heart, liver, ventral and dorsal skin, testis, stomach, intestine, urinary bladder and lungs of adult male Xenopus laevis toads Poly(A)+ RNA extracted from rat testes and...
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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx
Ngày tải lên :
07/03/2014, 15:20
... fully active alpha isoform and other variants, we conducted a series of cotransfection experiments and compared ligand-induced accumulation of cAMP (Fig 3) Although the level of cAMP accumulated ... boxes, insertion of a cryptic exon (B) The structure of constructs used for functional assays (C) The structure of constructs used for Western blotting In general the majority of our isoforms were ... with internal deletions and unusual isoforms composed of soluble extracellular (ligand-binding) domains [18] As the skin shows polymorphism in CRFR1 expression and its functional diversity may...
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Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt
Ngày tải lên :
08/03/2014, 10:20
... release more than one mole of adenine from each mole of ribosomes in 10 and even up to 12 mol of adenines in 40 (Fig 4B) Base and position specificity of lamjapin in depurination of SRD RNA Synthetic ... 10 lL of a solution of 0.2 M NaCl containing 100 lL of carrier tRNA and 40 lL of ethanol was added to the sample and kept at °C for 60 The mixture was centrifuged (15 000 g for 20 min) and the ... biosynthesis, and still in the range of the IC50 (0.03–4 nM) of type I RIPs The IC90 (the concentration of RIP causing 90% inhibition of translation) of lamjapin in the cell-free system of rabbit...
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Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx
Ngày tải lên :
16/03/2014, 11:20
... Rao and Dr J Josse for critical review of the manuscript, and Professor W Loffelhardt (University of Vienna, Austria) for ¨ his assistance in N-terminal protein sequencing and some MALDI-TOF MS ... molecular mass of 19 216 Da and a pI of 4.69, in good agreement with MALDI-TOF MS (Fig 1A) and chromatofocusing data, respectively The other three N-terminal sequences determined in this work, of Anabaena ... UÆmg)1 protein and recovery levels of 20– 30% In all cases, the analysis by SDS ⁄ PAGE and native PAGE of purified preparations showed only one protein band of 20–22 kDa (Figs and 2; supplementary...
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Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
Ngày tải lên :
16/03/2014, 18:20
... sites of NPAs as highly nonpolar and completely isolated from the solvent Here we analyze the conformational and functional properties of Ag-NPA-1 as well as the tissue and cellular distribution of ... metabolism and have to import complex lipids from the host [5] Nematodes possess two classes of structurally novel types of helix-rich LBPs [6–8] The first class consists of small 15 kDa fatty acid and ... chromophore of ABA-1, the single Trp of Ag-NPA-1 is a sensitive marker of the protein conformation and its emission reflects conformational changes after ligand binding Analysis of FRET from the...
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Báo cáo khóa học: Structural and functional comparison of 15S- and 15R-specific cyclooxygenases from the coral Plexaura homomalla potx
Ngày tải lên :
23/03/2014, 12:20
... (Figs and 3, and Table 2) Equivalent amounts of wild-type and mutant proteins as quantified by Western analysis gave similar conversion of the radiolabelled substrate The Val349Ile mutant of the ... Hamilton, R.D & Rhuland, L.E (1972) Occurrence of esters of 15S-prostaglandin A2 and E2 in coral J Am Chem Soc 94, 2122–2123 17 Light, R.J & Samuelsson, B (1972) Identification of prostaglandins in the ... numeration of ovine COX-1), are marked In the novel COX sequence, 17 amino acids that are different from the 15R-COX of P homomalla from the Florida Keys are boxed, and the respective residues of the...
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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx
Ngày tải lên :
23/03/2014, 13:20
... described should allow the isolation of large amounts of pure Ndh complex from maize chloroplasts for further structural and functional studies This may include identification of further Ndh subunits, ... exists as a monomer and dimer and splits into a membrane and soluble subcomplexes Separation of the Ndh complex by CN / PAGE (1D) and Tricine / PAGE (2D) and CN / PAGE (1D) and BN / PAGE (2D) ... GELGIYLVGDDSLFPWR and IRPPGFINLQILPQLVK (one missed cleavage) All of these peptides were part of the maize NdhH subunit Database analysis of a MALDI-TOF-MS measurement of a 21-kDa trypsin-digested gel band...
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Báo cáo khoa học: Isolation and identification of antimicrobial components from the epidermal mucus of Atlantic cod (Gadus morhua) docx
Ngày tải lên :
23/03/2014, 15:21
... Institute of Iceland and 50 specimens were caught randomly After killing the fish by a concussion of the brain by striking of the cranium, mucus samples were collected by scraping the skin and then ... of fragments thereof Hiemstra et al [44] isolated a small (6654-Da) antimicrobial cationic protein from the cytosol of interferon (IFN)-c-activated mouse macrophages, designated ubiquicidin and ... organization, and expression of a functional gene Gene 126, 227–235 24 Fernandes JM & Smith VJ (2004) Partial purification of antibacterial proteinaceous factors from erythrocytes of Oncorhynchus...
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