isolation and expansion of adult cardiac stem progenitor cells in the form of cardiospheres from human cardiac biopsies and murine hearts
Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... cells and the emission spectra of these particles peaked at 650 nm, thus confirming the identity and presence of QDs in the cells The labeling efficiency of QDs in whole BMCs (79.9 ± 3.4%) and Lin- ... these Lin- PCs migrated into the airspaces (approximately 3.1% of all the cells remaining in the lung), therefore approximately 14.6% of the initially infused Lin- PCs homed to lung tissues and ... as well as the participation of progenitor cells in the repair response of the lung [18-21] We propose that during infectious inflammation of the lung, these BM-derived progenitor cells could...
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Báo cáo khoa học: Hypoxia-driven proliferation of embryonic neural stem ⁄ progenitor cells – role of hypoxia-inducible transcription factor-1a ppt
... used in the present study Generally, neural stem cells were grown to form as neurospheres in vitro The ability of stem cells to form neurospheres is one of the indicators for proliferation of neural ... Bromodeoxyuridine (BrdU) into growing (DNA-synthesizing) S phase cells is more accurate in determining phase fractions We further used BrdU incorporation to determine whether hypoxia affects the DNA synthesis ... 1%, rather than arresting the growth of most kinds of cells, promote the proliferation of some types of cells There is increasing evidence that mild hypoxia acts as a potent regulator of various...
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báo cáo hóa học:" Isolation and culture of fibroblasts from endoscopic duodenal biopsies of celiac patients" pdf
... part in the intercellular network through the presence on their cell membrane and in the intracellular space of different types of receptors (receptors for E series of prostaglandins, insulinlike ... source of primary intestinal cells In this study, for the first time, we describe a suitable technique to obtain long-standing primary human FB cultures from endoscopic biopsies In the absence of ... Institutes of Health (Bethesda, USA) Before the analysis, an automated threshold process was performed on the images to minimize the influence of light variation in the microscope field and in the operator...
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Báo cáo y học: "Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus" pot
... background, was in charge of the recruitment of patients and participated in design and drafting of the manuscript MH participated in the design and coordination of the study and helped to draft the manuscript ... analysis and drafting of the manuscript KL was in charge of the recruitment of patients and their demography and performed the ELISA measurements XS participated in the culturing PBMNCs and functionality ... domain Caspase 8L binds to the Fas-associating protein with death domain (FADD) and blocks the binding of caspase to FADD by competiive inhibition [39] Indeed, in hematopoietic stem cells and...
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Báo cáo y học: " Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4" doc
... generation of induced pluripotent stem cells (iPS) [11-13] We and others have determined that Sall4 exists as two isoforms (Sall4A and Sall4B), and they have unique and overlapping functions [14-16] Interestingly, ... Interestingly, Sall4 is one of the few genes that are also involved in adult tissue stem cells [17,18], and its protein expression is always correlated with the presence of stem and progenitor ... not convert cells to cytokine independence In the combination of cytokines, the Sall4A or Sall4B transduced cells exhibited a ~2 fold increase in the rate of proliferation relative to the GFP control...
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Báo cáo y học: "Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage" potx
... [5,22] It is therefore possible that the initial expansion of MPCs for weeks in the present study led to a relative increase of the cells and an overestimation of the percentage of MPCs in native ... within the cartilage matrix Besides quantifying the relative percentage of CD166+ chondrocytes by immunofluorescence, analyzing the zonal distribution of these cells within the matrix of normal and ... and colleagues [21] (see Materials and methods) Interestingly, the distribution of the CD166+ cells in OA and normal samples was not uniform but was clearly zonal (Figures and 7) In both OA and...
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Báo cáo y học: "The change and effect of endothelial progenitor cells in pig with multiple organ dysfunction syndromes" ppt
... not investigate the correlation between the number and capacity of EPCs and the other factors of MODS in the present study The mechanisms for the decrease in the number and function of EPCs in the ... prior to the occurrence of MODS, so the sharp decrease in the number and function of EPCs in the progression of MODS may be one of the main pathogenic factors of MODS In the present study, the migratory, ... function of EPCs in peripheral circulation and in the bone marrow in MODS, and found that the number and function of EPCs would decrease in the progression of MODS and may be one of the main pathogenic...
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Tài liệu Báo cáo khoa học: Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas From target to transcript ppt
... Levels of MIH and CHH in the sinus gland during the moult cycle As individual SG from single crabs always contained almost identical profiles and quantities of peptides (preliminary experiments using ... traps in the Menai Strait, UK, and maintained in a recirculating seawater system under ambient conditions MIH and CHH were purified from sinus gland extracts by HPLC and quantified by amino acid ... of MIH and CHH on the target tissue, receptor binding, a second messenger pathway, MIH and CHH peptide and transcript levels in the XO with reference to the moult stage of the crustacean During...
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Báo cáo khoa học: A distinct sequence in the adenine nucleotide translocase from Artemia franciscana embryos is associated with insensitivity to bongkrekate and atypical effects of adenine nucleotides on Ca2+ uptake and sequestration pdf
... [37] Mindful of (a) the well-established ligand profile of ANT, (b) the modulatory role of ANT in the mammalian PTP, and (c) the absence of a Ca2+-induced PTP in mitochondria from the embryos of ... absence of the ANT inhibitory ligand cATR, and of the F0F1ATP synthase inhibitor oligomycin The results are shown in Fig In Fig 1A, ADP was present prior to the addition of mitochondria in all ... smaller in the latter case (see Fig 5B); however, the effect of alamethicin in mitochondria treated with ADP was not as great as the effect of the peptide in the absence of the nucleotide, and therefore...
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Pulmonary tuberculosis associated with increased number and percentage of natural killer and B cells in the peripheral blood pot
... numbers and + + percentage of CD3 CD16 CD56 NK cells detected in patients with PTB in this study may therefore indicate the contribution of NK cells in the host immune response against MTB infection ... experiments in mice have unveiled the role of B cells in the optimal host response against MTB by increasing the infection inoculum (Vordermeier et al., 1996; Maglione et al., 2007) In addition the protection ... associated CD4 + lymphopenia observed in the present study and in other studies require further investigation to gain a better understanding of the mechanism(s) and factors involved REFERENCES Aktas E,...
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báo cáo hóa học:" Serous papillary adenocarcinoma possibly related to the presence of primitive oocyte-like cells in the adult ovarian surface epithelium: a case report" docx
... VASA-positive cells (arrows) in the ovarian surface epithelium and cortex (A, B) Smaller cells with diameters of to μm in the epithelium (C-F) Bigger cells with diameters of 10 μm in the epithelium ... oocyte-like cells were released from their “chambers” in the ovarian surface epithelium and started to change into hypertrophic/necrotic cells (Figures and 6) and further into autofluorescent ... oocyte-like cells in the adult human ovarian surface epithelium as related to epithelial ovarian cancer These cells resembled primitive oocytes in the ovarian surface epithelium of fetal ovaries, and...
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Báo cáo khoa học: "Comparative study of endocrine cells in the principal pancreatic islets of two teleosts, Silurus asotus (Siluridae) and Siniperca scherzeri (Centropomidae)" potx
... 1998), insulinimmunoreactive cells were found in the central regions of the principal pancreatic islets of both species in the present study Glucagon is synthesized in the A cells of the pancreas and ... study of the endocrine cells within the pancreas, intestine, and stomach of the gar Gen Comp Endocrinol 1997, 106, 1-16 Guyot, E., Diaz, J P., Romestand, B and Connes, R Insulin during the early ... isolated from the hypothalamus of sheep, and exists in straight and cyclic forms (Brazeau et al., 1973) This substance inhibits the secretion of the gastrin, cholecystokinin, secretin, glucagon, insulin,...
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Báo cáo khoa học: " An immunohistochemical study of chromogranin A and Sp-1 immunoreactive cells in the gastrointestinal tract of ovariectomized rats" pdf
... htiw demrofrep saw noitcaer cificepsnon fo gnikcolB ]13[ dohtem )PAP( esadixorep itna-esadixorep eht htiw deniatsonummi dna detardyher ,deziniffaraped saw noitces evitatneserper hcaE gniniats lacimehcotsihonummI ... etanobracylop rep detacolla erew slaminA syad rof noitazitamilcca retfa desu erew )napaJ ,reviR selrahC ;tpiecer nopu dlo kw-6( tar elamef )DS( yelwaD-eugarpS ytnewT o slamina latnemirepxE sdohteM dna ... lanoitubirtsid eht ,hguohtlA ]8,3[ sllec enircodneoruen lla fo ”srekram“ nommoc yltnacifingis a ,stneitap aitreni cinoloc nI slamina fo sutats evitsegid ot detaler ylraelc erew sllec RI-GC fo segnahc eht...
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Báo cáo y học: "Regulating the immune system: the induction of regulatory T cells in the periphery Jane H Buckner1 and Steven F Ziegler2" pot
... whether the CD4+CD25– T cells capable of differentiating into TR cells following activation are derived from a separate lineage of CD4+ T cells, and whether their induction is a result of the initial ... involution, and antigen exposures, the development of CD4+CD25+ TR cells may occur in the periphery in man Induction of CD4+CD25+ TR cells in the periphery The induction of TR cells that resemble the ... T cells through coengagement of CD3 and the complement regulator CD46 in the presence of IL-2 These conditions resulted in IL-10producing cells capable of inhibiting the activation of bystander...
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Báo cáo y học: "Dendritic cells: In the forefront of immunopathogenesis and vaccine development – A review." doc
... great insights into the origins of DC subsets [6,7] and their modulation by distinct cytokines of neighboring cells [8,9] Progenitors of DCs in bone marrow migrate via the blood stream and home ... antigen-specific B cells [29] T- and B-cell interaction results in the clonal expansion of B cells, which takes place in the plasma foci of the T cell rich area [30] and in the germinal centers [31] T- and ... immunogen in the intestine DCs in the mucosal site will then capture the immunogen via DC-binding peptide motifs They internalize the immunogen, process and present it to T cells inducing specific...
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báo cáo khoa học:" Multimorbidity and health-related quality of life in the older population: results from the German KORA-Age study" ppsx
... manuscript BT was involved in the coordination of the study and commented on drafts of paper MS was involved in the interpretation of data AD participated in the coordination of the study PM commented ... drafts of paper AP was involved in the conception of the study RH was involved in the conception of the study and assisted in writing the manuscript All authors have read and approved the final ... where Yi is the response of individual i, fage and fBMI are smooth functions, and xiTb is a linear predictor including the binary and categorical covariates of the model As in the linear model,...
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Báo cáo y học: " Profibrotic potential of Prominin-1+ epithelial progenitor cells in pulmonary fibrosis" docx
... Bleomycin promotes the accumulation of prominin-1+ progenitors in the injured lung Inflammation mobilizes and activates local or exogenous stem and progenitor cells We therefore investigated whether ... cells (A, right) Further, prominin-1positive cells were isolated using magnetic cell sorting and cultured in the presence of different cytokines and growth factors Prominin1+ cells cultured in ... bleomycin instillation promotes the accumulation of bone marrow-derived prominin-1+ progenitors in the lung So far, we have identified two distinct populations of prominin-1 expressing cells in the...
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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps
... cytokine for Tregs, and plays a key role in the control of self-antigen-reactive T cells in vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our ... both inflammation and tissue damage One of the critical mediators of inflammation in RA is the proinflammatory cytokine IL-6 Interestingly, the UC-MSCs could downregulate the IL-6 production in the ... profound inhibitory effect on FLSs and T cells from RA patients They could suppress proliferation, the invasive behavior and inflammatory responses of FLSs, inhibit activation of T cells and induce...
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Báo cáo y học: "Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver" pptx
... fat-storing cells or perisinusoidal lining cells Debate exists regarding the origin of portal and interface MF and HSC They may have a common origin in the primitive mesenchyme of the embryonal septum ... staining in the perisinusoidal spaces throughout the parenchyma b) HSC stain positive, producing a thin irregular positive band lining the sinusoids c) HSC stain positive A positive cell containing ... insight in normal baseline variation In the formalin fixed paraffin embedded normal canine liver vimentin staining did not differentiate between fibroblasts and MF in the portal area and the perivenous...
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The role of CD8 t cells in the differentiation of TNF iNOS producing dendritic cells and TH1 responses
... infected cells CD8 T -cells recognizing such cells would then release cytotoxic granules resulting in the killing of 39 Chapter 1: Introduction these infected cells Under these circumstances, the dying ... T cells (Steinman and Cohn, 1974) These cells are now known as the primary instigators of the adaptive immunity as they are vital for detecting, alerting and priming the adaptive immune system ... perforin and granule enzymes granzyme A and B (Berke, 1995) They then induce cytolysis of infected cells either by the granule exocytosis pathway through co-ordinated delivery of perforin and granzymes...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng 9 tr 25