investigating genes that are up regulated in mgh cell line after 2 hours lyo bcg treatment using representational differential analysis rda

Mycobacterium bovis, BCG modulation of gene expression in bladder cancer

Ngày tải lên : 11/09/2015, 10:06

... Investigating genes that are up- regulated in MGH cell line after hours Lyo BCG treatment using Representational Differential Analysis (RDA) …………………… 40 2. 2.3 α5β1 analysis and BCG internalization ... regulation in MGH cell line after treatment with cultured BCG or lyo BCG for hours …………………………………………… 96 4 .2. 4.1 Pathway specific microarray analysis of genes induced in MGH cells after BCG treatment ………………………………………………………………………… ... RT-PCR analysis of GSTT2, TNFα and IL-1β expression in J 82 cell line …… 1 02 4 .2. 5 Blocking direct BCG interaction with a transwell device………………………… 1 02 4 .2. 5.1 Blocking BCG interaction with MGH cells...

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PURIFICATION OF ORGANIC CHEMICALS pot

Ngày tải lên : 05/03/2014, 21:20

... alkaline in H20 and the pKa:5 is 12. 1 It has A,, 22 4nm (E 4000) in H20 The picrate has m 25 2O (sintering at -24 5O) [Dox Org Synfh Coll Vol I 1941; Davies and Parsons Chernisfry and Industry 628 ... M 62. 0, b 20 S0/760mm, d 1.0 32 Purified by fractional distn N-Acetyl-D-galactosamine [1 421 5-68-01M 22 1 .2, m 160-161°, [a1546+102O (c 1, HzO), N-Acetyl-D-glucosamine [75 12- 17-61 M 22 1 .2, m ca 21 S0, ... [ 123 5 -21 -81 M 354.8, m 23 7 -23 8", 24 4 -24 6O CHC13 + C6H6 + pet ether (b 60-80") and by dissolving in CHC13and running the soln (dec) Recrystd into dry Et,O h m nm(E) 25 5(3,600), 26 2(3,700), 26 8(4,000)...

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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc

Ngày tải lên : 07/03/2014, 21:20

... 41(67) 23 6(M1 ,26 ), 135 (28 ), 121 (100), 119 (29 ), 107(41), 93(40), 91 (29 ), 69(33), 55(44), 43 (28 ), 41(46) 21 8(M1,8), 136(49), 135(71), 121 (24 ), 107(100), 105 (20 ), 91(33), 79 (20 ), 67( 52) , 53 (22 ), 41 (28 ) ... acetate (5:3 :2) n-Heptane/acetonitrile/ethyl acetate (2: 1:1) K2 K3 K4 K5 K6 0.39 0.60 0.77 1.49 1.66 3 .27 0.33 0.56 0.76 1.74 1.94 4.53 0.17 0. 32 0.50 1. 02 1.15 2. 22 0 .28 0.54 0. 72 2.08 2. 17 6.49 ... broadening [7, 29 ] In this study, K Aup (peak area of compound in upper phase)/Alow (peak area of compound in lower phase) Previous study indicated some components degraded in aqueous solution [26 ]...

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PURIFICATION OF LABORATORY CHEMICALS pot

Ngày tải lên : 14/03/2014, 20:20

... celluloses and sephadex chromatography) 18 18 18 19 19 20 20 20 20 21 21 21 21 21 21 22 22 23 23 24 Sephadex Sepharose Cellex CM Cellex D Crystalline hydroxylapatite Gel filtration High performance ... chromatography 24 24 25 25 25 25 25 26 27 ELECTROPHORESIS 27 DRyING Removal of solvents Removal of water Intensity and capacity of common desiccants Suitability of individual desiccants Molecular sieves 27 ... MODIFIED FIBROUS CELLULOSES FOR ION-EXCHANGE 43 BEAD FORM ION-EXCHANGE PACKAGINGS 44 TABLE TABLE 15 16 TABLE TABLE TABLE TABLE TABLE TABLE 17 18 19 20 21 22 TABLE 23 TABLE 24 TABLE TABLE 25 26 LIQUIDS...

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purification of carbon nanotubes

Ngày tải lên : 20/03/2014, 13:06

... storage media [16,17] and nanoelectronic devices [18,19] 1.1 20 14 20 14 20 15 20 16 20 16 20 16 20 17 20 18 20 18 20 18 20 20 20 20 20 21 20 21 20 21 20 21 20 21 benzene, ethanol, acetylene, propylene, methane, ethylene, ... Therefore, purifying CNTs using H2O2 followed by HCl treatment to remove metal particles has also been intensely investigated Macro-scale purification, including a first refluxing treatment in H2O2 solution ... [114] [115] [116] [117] [118] [119] [ 120 ] [ 121 ] [ 122 ] [ 123 ] [ 124 ] [ 125 ] [ 126 ] [ 127 ] [ 128 ] [ 129 ] [130] by size-exclusion chromatography Anal Chem 20 05;77(19): 622 5–8 Doorn SK, Fields RE, Hu H, Hamon...

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Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot

Ngày tải lên : 29/03/2014, 09:20

... Superose 636 24 5 74 61 27 5 65 31 15 10 129 14 5.4 2. 1 2. 9 1 .2 660 13 1.3 0 .26 927 24 3.0 0.3 0.13 1811 71 3.7 0. 42 0.36 0.063 0.96 19 59 23 9 0.30 2. 7 10 51 79 0.071 0.19 1.4 5.0 8.0 19 20 61 24 8 ... Thermococcus litoralis [22 ] The order of divergence of AT3 from enzymes in subfamily Ic is ambiguous in Fig Asp 2- OG AT1 OAA AT2 Pyr Glu Glyo 2- OG AT2 & AT1 2- OG Gly Ala Glyo Pyr AT3 Fig Aminotransferase ... and Arg 329 in the E coli PSOT are involved in the interaction with the negatively charged phosphate group of the phosphoserine These residues are conserved not in AGTs, but are found in all PSOTs...

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armarego - purification of laboratory chemicals 5e

Ngày tải lên : 04/06/2014, 15:52

... 197 20 3 21 2 21 9 22 3 23 5 24 4 25 1 25 7 26 7 27 5 29 0 3 02 318 330 340 349 356 359 361 26 2 115 128 141 150 156 161 176 193 20 4 21 2 21 8 22 8 23 5 23 8 25 1 26 0 26 8 27 4 28 4 29 3 308 320 337 350 360 368 376 27 9 ... 123 128 136 1 42 144 155 1 62 168 174 1 82 189 20 1 21 1 22 5 23 5 24 4 25 1 25 7 25 9 26 1 26 2 56 67 78 85 90 94 108 122 131 137 143 151 157 160 171 179 185 190 199 20 6 21 9 22 9 24 3 25 4 26 3 27 0 27 7 27 9 28 1 ... 28 2 68 79 91 98 103 108 121 136 146 1 52 158 166 173 176 187 195 20 2 20 7 21 6 22 3 23 7 24 7 26 2 27 3 28 2 29 0 29 6 29 9 301 3 02 80 91 103 111 116 121 135 151 160 167 173 1 82 188 191 20 3 21 1 21 8 22 4 23 3...

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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot

Ngày tải lên : 18/06/2014, 18:20

... inocula containing about 1.3 × 108 DNA viral copies of HHV-6A (U11 02) per 106 cells After h incubation, cells were washed and maintained in RPMI containing 10% FCS for 24 h The cells were washed ... processing software Analysis (Soft Imaging system GmbH, Munster, Germany) Metabolic labelling The cells were infected for h and maintained in RPMI containing 5% FCS for 21 or 69 h The cells were ... thin gradient fractions infected cells and of particles in EM thin section analyses of infected cells and of particles in iodixanol gradient fractions A HHV-6A infected cells at dpi Indicated are...

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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx

Ngày tải lên : 20/06/2014, 01:20

... inocula containing about 1.3 × 108 DNA viral copies of HHV-6A (U11 02) per 106 cells After h incubation, cells were washed and maintained in RPMI containing 10% FCS for 24 h The cells were washed ... processing software Analysis (Soft Imaging system GmbH, Munster, Germany) Metabolic labelling The cells were infected for h and maintained in RPMI containing 5% FCS for 21 or 69 h The cells were ... thin gradient fractions infected cells and of particles in EM thin section analyses of infected cells and of particles in iodixanol gradient fractions A HHV-6A infected cells at dpi Indicated are...

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Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc

Ngày tải lên : 20/06/2014, 01:20

... Randal Schoepp kindly provided the following LASV-specific mAbs: NP-specific mAbs 522 73-8 and L2-54-6A; GP1-specific mAb L 52- 74-7A; and GP2-specific mAbs L 52- 2 72- 7, L 52- 121 -22 , and L 52- 2 727 , which ... System pMAL-p2x:GP1 pMAL-p2x:GP2 pMAL-p2x:NP pMAL-c2x:GP1 pMAL-c2x:GP2 pMAL-c2x:NP GP1 GP2 NP GP1 GP2 NP Rosetta 2( DE3) Rosetta 2( DE3) Rosetta 2( DE3) Rosetta gami Rosetta gami Rosetta 2( DE3) Page ... strains used to express the recombinant LASV genes are outlined in Table Briefly, initial pilot expression studies were performed with vectors pMAL-p2x:GP1, pMALp2x:GP2, and pMAL-p2x:NP in the...

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Báo cáo hóa học: " Temperature sensitive influenza A virus genome replication results from low thermal stability of polymerase-cRNA complexes" doc

Ngày tải lên : 20/06/2014, 01:20

... Yao Y, Mingay LJ, McCauley JW, Barclay WS: Sequences in influenza A virus PB2 protein that determine productive infection for an avian influenza virus in mouse and human cell lines J Virol 20 01, ... domains of the influenza A virus PB2 protein: identification of NP- and PB1binding sites Virology 20 04, 321 : 120 -133 Mullin AE, Dalton RM, Amorim MJ, Elton D, Digard P: Increased amounts of the influenza ... all subsequent binding of the polymerase and displace non-specific RNA binding of other proteins in the extract The binding reactions were then aliquoted into 12 separate tubes and incubated at...

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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx

Ngày tải lên : 21/06/2014, 02:20

... AZ1 323 4) for financial support and Christin Peters and Ina Menyes for assistance in the laboratory Competing interests The authors declare that they have no competing interests Received: June 20 11 ... primer annealing, 70 s, 72 C elongation The final elongation step was performed over 10 minutes at 72 C The resulting 10 92 kb fragment was digested with NdeI and XhoI and ligated into pET -28 b digested ... Vector 2, 5-DKCMO_pET -28 for expression of recombinant 2, 5-DKCMO from P putida NCIMB 10007 under control of T7 promoter in E coli BL21 The 2, 5-DKCMO-gene was introduced using the sites of restriction...

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purification of some heavy metals from paper making

Ngày tải lên : 20/07/2014, 12:47

... Sum of Squares df Mean Square F value P value Between Groups 4.8 72 1 .21 0 4. 12 0. 028 * Within Groups 2. 963 12 Total 7.835 16 Generally speaking, removal rate of reed has the same trend in different ... growing seasons are divided into germination period, leaf extension period, jointing period, heading to flowering and mature period According to these growth periods, five experimental basins are ... nickel, copper pollution in water[J].China's mining, 20 09,18(1):69- 72 [13] Liu J.Study on the Main Pollutant Fluxes Flowing into the Bohai [D].China Ocean University ,20 06 [14] Wang X P Different...

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báo cáo khoa học: "Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation" pps

Ngày tải lên : 11/08/2014, 00:23

... 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Gaber-Porekar V, Menart V: Potential for using histidine tags in purification of proteins at large scale chemical engineering technology 20 05, 28 :1306-1314 ... 65.7 12. 8 14.9 3.9 1.9 33.5 51 32. 2 5 .2 0.5 12 0.5 1 .2 4.1 6.8 57 33.5 0.8 1 .2 8.9 13.5 24 .4 73 9.1 46 63.9 4.1 4.1 2. 6 2. 6 11.7 18.5 11.7 27 .4 36.9 45.8 80 72 9.1 18.1 65.7 4.1 2. 6 11.7 27 .3 ... nickel ions tightly integrated in the silica [11] and so, in contrast to NTA-coupled matrices, all valences of Ni are available for histidine binding This may result in increased binding to His-like...

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Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx

Ngày tải lên : 11/08/2014, 21:21

... demonstrate that any new diagnostic technology using recombinant PPV VP2 has advantages over current diagnostic tests and that any new vaccines against PPV using VP2 are superior to current vaccines in ... expressed PPV VP2 in E coli using the plasmid pET-32a (+) [17] VP2 expressed in bacteria had similar antigenicity to native PPV VP2, as determined by Western blot analysis using polyclonal antibodies ... obtained from the Harbin Veterinary Research Institute, CAAS The serum samples were collected from an outbreak of PPV in 20 08 in one farm in Heilongjiang Province 2. 2 Construction of recombinant...

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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt

Ngày tải lên : 12/08/2014, 04:21

... activity can be increased in HIV-infected individuals receiving p24 and the antiviral drug zidovudine, reinforcing the possibility for a p24-containing therapeutic vaccine against HIV in the presence ... other immunizations M p24 kDa 95 72 M Anti-HIS M anti-Ricin 72 55 Anti-p24 kDa 55 95 43 36 36 28 34 28 17 26 6xHIS C B p24 6xHIS RTB p24 Figure of recombinant proteins expressed in E coli and immunodetection ... the fusion protein RTB/p24 retained lectin activity and therefore, it is likely that after i.n administration, binding to M cells by RTB increased the uptake and transport of p24 to the nasal...

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PURIFICATION OF SIMPL ANTIBODY AND IMMUNOFLUORESCENCE OF SIMPL SUB-CELLULAR LOCALIZATION IN RESPONSE TO TNFα- AND IL-1

Ngày tải lên : 24/08/2014, 12:43

... p65 in the nucleus of endothelial cells 20 Discussion 22 A 23 SIMPL’s effectiveness in the purification of SIMPL antibody 22 B The effectiveness of washing an AminoLink ... cytokine conditions were 20 capable of inducing an inflammatory response in the endothelial cells, the localization of NF-κB was monitored in control groups of cells Two groups of endothelial cells ... BSA Bovine serum albumin BME β-mercaptoethanol CAK1 Cyclin dependent protein kinase-activating kinase dd double distilled DAPI 4’,6-diamidino -2- phenylindole, dihydrochloride ELISA enzyme-linked...

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studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene

Ngày tải lên : 13/11/2014, 09:25

... CHITINASE-CONTAINING PROTEIN COMPLEX IN THE STRAWBERRY PLANT 2. 1 Introduction Plant chitinases are pathogenesis-related (PR) proteins, which are implicated in plant defense mechanisms against ... is also involved in the feedback regulation of SA biosynthesis during SAR (Cao et al., 1997) NPR1 is an ankyrin-repeat containing protein, a domain often involved in protein-protein interactions ... They are membrane-localized proteins, and are homologs of the bacterial two-component histidine kinases involved in sensing environmental changes (Wang et al., 20 02) Interestingly, it appears that...

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purification of phytase from aspergillus fumigatus

Ngày tải lên : 06/10/2015, 12:59

... phytase, SP-Streamline i CONTENTS Abstract i CONTENTS ii INTRODUCTION MATERIALS AND METHODS 2. 1 Materials 2. 2 Methods 2. 2.1 Preparation of fungi strain and culture medium 2. 2 .2 Extraction of the ... chosen from 2. 2.3.1, centrifuged at 7000 rpm for 20 minutes at 4°C The obtained pellet was dissolved in Tris-HCl 0,02M pH 7.6 and dialyzed against the same buffer in the fridge for 24 hours After dialysis, ... from 2. 2.3.1, and centrifuged at 7000 rpm for 20 minutes Dissolving the pellet in the buffer Tris-HCl 0.02M pH 7.6 + AS 30% saturation, and dialyzed against the same buffer in the fridge for 24 hours...

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THE CONTRIBUTION OF CLAMS ON TIDAL FLAT PURIFICATION CAPACITY

Ngày tải lên : 05/09/2013, 09:08

... used immediately after sampling, while the samples for quinone were stored at 20 oC until using for the analysis - 84 - Journal of Water and Environment Technology, Vol .2, No .2, 20 04 Laboratory ... Seed and mineral solutions were also added The flasks were shaken and incubated in the dark condition at 22 ± 2oC Decomposition was followed by DOC analysis at frequent interval over a 28 -day period ... from the sediment using a mixture of chloroform-methanol and then re-extracted into hexane Menaquinones and ubiquinones contained in the crude extract were separated and purified using Sep-Pak® Plus...

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