... the interactionof TATAbox binding protein and TFIIA with DNA. Biochemistry 33,7033–7040.4760 E. Trotta et al. (Eur. J. Biochem. 270) Ó FEBS 2003 Interaction of DAPI with individual strands of ... compact structure of DAPI-bound ATT-template with respect to its free form, which is consistent with the previously reported lower mobility of the freemonomeric random coil structure of (ATT)6in ... primer-complementary region of the template. (C) Chemical structure of DAPI.4756 E. Trotta et al. (Eur. J. Biochem. 270) Ó FEBS 2003the interactionwith DAPI. This suggests an efficientmechanism of single-stranded...
... production. 2.1 Categories of production Production can be done in one of two directions, or by combining both: "extraction" and "synthesis". Some common categories of ... function model of CLOS). Finally, the values of the output variables of the left-hand side nonterminal (aol, ao2 ) are collected and returned as the result of its expanding. With some predefined ... and production make the notion of lexical accumulation complete: the former is to obtain lexical data of (almost) the same linguistic structure as the source, the latter is to create data of...
... conflict of interest, or components thereof, that might pertain to conflict of interest in compendia; and (2) published reports of conflict -of- interest issues with regard to the writing of guidelines ... practices of four specific compendia (those officially approved for use in making Medicare coverage determinations) with regard to conflict of interest; and (4) a discussion of the adequacy of compendia ... 20 4.1.2 Conflict of interest in data availability 23 4.1.3 Conflict of interest in selection of indications and evidence 24 4.1.4 Conflict of interest in interpretation of evidence 26 4.2...
... into the interactionof human arginase II with substrate and manganese ions by site-directedmutagenesis and kinetic studiesAlteration of substrate specificity by replacement of Asn149 with AspVasthi ... spite of the rel-atively low agmatinase activity of the Asn149Asp vari-ant, it is clear that the interactions of arginase II with l-arginine and agmatine are greatly altered by replace-ment of ... bluestaining of purified enzymes.Metal-free species of purified enzymes were obtained byincubation for 1 h at 25 °C with 25 mm EDTA and 3 mV. Lo´pez et al. Interactionof arginase II with substrate...
... spectra of a recombinant decorin purified in theabsence of chaotropic agents, with the exception of thewavelength of the minimum (215–216 instead of 218 nm)and very different to the spectrum of ... 2002 Interactionof decorin with collagen peptides (Eur. J. Biochem. 269) 1431 with respect to the unmodified ones is not related to thepercentage of Lys/Hyl side chains that did not react with ... these data demonstrate the essential role of the positive charge of collagen Lys/Hyl residues for interaction with decorin.DISCUSSIONThe binding of decorin with fibrillar collagens has beenextensively...
... treatment, whereas stimula-tion with LPS only induced significant enhancement of superoxide production after a period of 6 h of stimula-tion (data not shown).The effect of muranyl peptides on the ... the overexpression of UCP2protein correlated with the productionof both reactivespecies. However, cells stimulated with MB did notpresent any modification in the level of detectableROS or ... source of ROS production and the primary ROS generated issuperoxide anion as a consequence of monoelectronicreduction of O2. Moreover, the main sites of OÀ2genera-tion at the level of the...
... forthe interactionof frog annexin A1 and annexin A2 with dicalcin. Consistently, we observed that the35 kDa forms of annexin A1 and annexin A2 foundin the fraction of the binding proteins of dicalcin(Fig. ... presence of dicalcin and all of the annexins (E) was compared with the calcu-lated sum of each of the initial rates obtained in (B)–(D) (thick dot-ted lines).T. Uebi et al. Role of dicalcin ... 4869Comprehensive interactionof dicalcin with annexinsin frog olfactory and respiratory ciliaTatsuya Uebi1, Naofumi Miwa1,2,* and Satoru Kawamura1,21 Department of Biology, Graduate School of Science,...
... size-distribution of oligomersobtained after hydrolysis of chitosan (FA¼ 0.65) with ChiB todegrees of scission (a) varying from 0.03 to 0.37. Peaks arelabelled with DP-values or with the sequence of the ... Size-distribution of oligomers after extended hydrolysis of various chitosans with ChiB. Chromatograms showing the size-distribution of oligomers obtained upon extended ChiB-hydrolysis of chitosans with FA of ... 173–178.Degradation of chitosans with ChiB A. Sørbotten et al.548 FEBS Journal 272 (2005) 538–549 ª 2005 FEBSDegradation of chitosans with chitinase B fromSerratia marcescens Production of chito-oligosaccharides...
... means of regulation of a member of the guanylyl cyclase receptorfamily, by controlling the amounts of differentially glycosy-lated forms of GC-C in a cell. Given the fact that the130 kDa form of ... reappearance of the 145 kDa form of GC-C. Cells werecultured for 18 h in the presence of ST, ST was thenremoved and cells fed with serum-containing mediumwithout ST. At various times after renewal of ... absence of differentially glycosylated forms of GC-C.It is possible that the reappearance of the 145 kDa formfollowing removal of ST peptide was a consequence of further glycosylation of the...
... activation of trehalase. Datareported here indicate that following a heat shock theenzyme activity of trehalase is associated with Ntp1p dimersor trimers but not with either Ntp1p monomers or with complexes ... behavior of trehalasedepending on the nature of the activation stress (Fig. 5B).This shift might argue against the participation of a uniqueset of interactions involving association of Ntp1p ... identification of strains C3 andC69, with one copy of Ntp1p–Ha6H expressed from thegenomic ntp1+promoter, was verified by Southern blotanalysis and immunoblot of whole-cell extracts with anti-Ha...
... soluble Cu and Pb with increased desorption of HA is a consequence of the com-plexation of these metals by HA.4. ConclusionsDOC increases with increasing pH. The fractionation of DOC desorbed/dissolved ... desorption/dissolu-tion of HA is strongly correlated to the increase of theconcentration of Pb in solution. As both Cu and Pb formstrong complexes with HA (Tipping, 1994), it is likely thatthe correlation of increased ... de-creases with increasing extraction equilibrium pH. Thepercent FA tends to remain constant over the range of pHvalues. Within soils, the partitioning of FA and HA corre-lates with the system...
... dissociationwas observed with the A8B8isoform of GAPDH lead-ing to the A2B2isoform with a higher NADPH-dependent activity [39].By analogy with the CTE of the isoform of GAPDH[15,37], we ... date, mapping of the interaction site of CP12had not studied but our results give some clues with regard to the binding of CP12 to GAPDH. The impact of CP12 binding on the activity of GAPDH is ... dissociation resultsin the release of PRK as a dimer, whereas GAPDHremains strongly associated with CP12 and forms asubcomplex of one tetramer of GAPDH with onemonomer of CP12 [2,10]. However, in...
... independently of interactions with heat- shockprotein 90Emma L. Turnbull, Ina V. Martin* and Peter A. FantesInstitute of Cell Biology, School of Biological Sciences, University of Edinburgh, ... studies of the interactionof Cdc37 with Hsp90.J Mol Biol 340, 891–907.18 Siligardi G, Panaretou B, Meyer P, Singh S, WoolfsonDN, Piper PW, Pearl LH & Prodromou C (2002) Regu-lation of Hsp90 ... contain Hsp90. Identifica-tion of an interaction between Hsp90 and Cdc37 hasbeen problematic due to the instability of the inter-action and the small amount of Cdc37 that is presentin the...
... lLof6.5pMT4 DNA weremixedwith1lL of labeled C1q prepared as above, andaccording to case with 5 lLof45lMfucoidan; when thepurified C1q was used, 400 lLof6.5pMT4 DNA weremixedwith10 lL of ... region are involved in the assembly of C1 throughspecific interactionwith acidic residues of C1r [35].Furthermore, the structural model of C1q shows a cluster of basic residues that are located ... labeled-CLR (CLR*).Incubation of T4 DNA with CLR* in the presence of fucoidan (CLR/fucoidan molar ratio of 1 : 20 and 1 : 40)leads to an inhibition of the binding of CLR* to DNA fromthe molar...