interaction of electron beam with electromagnetic field
Báo cáo Y học: Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe pdf
... behavior of trehalasedepending on the nature of the activation stress (Fig. 5B).This shift might argue against the participation of a uniqueset of interactions involving association of Ntp1p ... identification of strains C3 andC69, with one copy of Ntp1p–Ha6H expressed from thegenomic ntp1+promoter, was verified by Southern blotanalysis and immunoblot of whole-cell extracts with anti-Ha ... the regulation of Ntp1p activity under stress relieson the existence of some kind of interaction between Ntp1pand Tps1p, or other elements, that may be lost under theconditions of the in vitro...
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PSPICE simulation of two mass vibratory conveying system with electromagnetic drive
... is a consequence of exertion of the mechanical sub-system on the electromagnetic sub-system. IV. MATHEMATICAL MODEL OF THE VCS Description of one type of two-mass electromagnetic vibratory ... half- wave with fd=50Hz. It has been simply realized with comparator tolerance band i.e. hysteresis (“bang-bang”) controller. Reference current was compared with actual current with the tolerance ... case of thyristor converter, with phase control, LCE displacement has “smooth” sine characteristic, although EVA current is pulsating. Also, change of vibratory width is due to change of phase...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... into the interaction of human arginase II with substrate and manganese ions by site-directedmutagenesis and kinetic studiesAlteration of substrate specificity by replacement of Asn149 with AspVasthi ... spite of the rel-atively low agmatinase activity of the Asn149Asp vari-ant, it is clear that the interactions of arginase II with l-arginine and agmatine are greatly altered by replace-ment of ... bluestaining of purified enzymes.Metal-free species of purified enzymes were obtained byincubation for 1 h at 25 °C with 25 mm EDTA and 3 mV. Lo´pez et al. Interaction of arginase II with substrate...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... intensity of the band of unhydrolyzed actin was plotted against the time of incubation.Characterization of actin preparation qualityFluorescence parameters were used for estimation of nativity of ... aggregation of partiallyfolded actin.To obtain more detailed information on the interaction of HSP25 with partially unfolded actin, we analyzed theeffect of different quantities of HSP25 on ... parameter A of actin was close to2.4. Increase of the time of heating at 43 °C leading todecrease of parameter A up to 2.2–2.3 was accompanied byfurther decrease of the initial rate of polymerization....
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Tài liệu Báo cáo Y học: Prediction of protein–protein interaction sites in heterocomplexes with neural networks ppt
... probableregions of interaction, in agreement with the contacts with GrpE and the results obtained from experiments with mutants. The contact r egions predicted w ith our methodand t he implicit model of interaction ... of the protein structure is the detection of regions of interaction by the presence of specific familysignatures in the m ultiple s equence alignment a ble t odiscriminate different t ypes of ... retained, independent of theirclassification. Furthermore, a 40% sequence identity cut-offfor protein homology is used instead of the present 30% andthe definition of the interaction surface is...
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Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot
... spectra of a recombinant decorin purified in theabsence of chaotropic agents, with the exception of thewavelength of the minimum (215–216 instead of 218 nm)and very different to the spectrum of ... 2002 Interaction of decorin with collagen peptides (Eur. J. Biochem. 269) 1431 with respect to the unmodified ones is not related to thepercentage of Lys/Hyl side chains that did not react with ... these data demonstrate the essential role of the positive charge of collagen Lys/Hyl residues for interaction with decorin.DISCUSSIONThe binding of decorin with fibrillar collagens has beenextensively...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... basallevels of resonance units (GlnK free surface) withinseconds. To test the effects of various molecules on the interaction of TnrA with GlnK, 40 nm GlnK (trimer)Fig. 1. Coimmunoprecipitation of TnrA, ... equilibrated with 10 column volumes(10 · 0.2 mL) of buffer B, with subsequent washing fourtimes with five volumes of the same buffer. Proteins wereeluted with buffer E (buffer B supplemented with 250 ... (SPR) of theGlnK–TnrA interaction As a next step, the interaction of TnrA with GlnK wasinvestigated in vitro by BIAcore SPR detection. Forthis analysis, a Strep-tag II-tagged variant of GlnK(GlnK-ST)...
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Báo cáo khoa học: Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia pdf
... forthe interaction of frog annexin A1 and annexin A2 with dicalcin. Consistently, we observed that the35 kDa forms of annexin A1 and annexin A2 foundin the fraction of the binding proteins of dicalcin(Fig. ... presence of dicalcin and all of the annexins (E) was compared with the calcu-lated sum of each of the initial rates obtained in (B)–(D) (thick dot-ted lines).T. Uebi et al. Role of dicalcin ... 4869Comprehensive interaction of dicalcin with annexinsin frog olfactory and respiratory ciliaTatsuya Uebi1, Naofumi Miwa1,2,* and Satoru Kawamura1,21 Department of Biology, Graduate School of Science,...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... Recognit. 16,91–101.Ó FEBS 2004 Interaction of human TCR with superantigen SSA (Eur. J. Biochem. 271) 4083Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen ... Vb5.2 and V b1withKd of 9–11 lM with a fast kassand a moderately fast kdiss. In spite of the reported stimu-lation of V b2.1 bearing T-cells by SSA, we o bserved nomeasurable interaction. Keywords: ... Consequently, the molecularstudies of the interaction of SAgs with their s pecifics ligandswill not only advance understanding of the physiologicalmechanisms of these molecules, but may lead to...
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Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc
... report on the interaction of anN-terminal (E2(7–26)) and an internal (E2(279–298))synthetic peptide sequence of the E2 structural pro-tein of HGV ⁄ GBV-C with phospholipid membranes of different ... short hydrophobic segments of viral envelope glyco-proteins with a very low content of hydrophilic aminoacids, the presence of acidic residues in the fusion pep-tides of some low-pH-activated ... strongly interacted with PtdCho ⁄ PtdSer (65 ⁄ 35) vesicles, as the blue shift of Trp was 11 nm.To study the contribution of electrostatic inter-actions to the binding of both peptides with negativelycharged...
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Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
... transmis-sion electron microscopy (TEM) and atomic forcemicroscopy [15].In this study, we measured the interaction of stefin B with various combinations of phospholipid monolayersand bilayers. Interaction ... channel hypothesis of AD and neurodegenerationin general, is not incompatible with other key elements of toxicity, as, for example, the deregulation of Ca2+homeostasis and generation of reactive ... membranes, initiating a cascade of detrimentalevents for the cell. Interaction of granular aggregates and globular oligo-mers of an amyloidogenic protein, human stefin B, with model lipid mem-branes...
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Báo cáo Y học: Interaction of the anterior fat body protein with the hexamerin receptor in the blowfly Calliphora vicina pot
... predicted proteinis composed of 306 amino acids, with a calculated molecularmass of 34.3 kDa and a pI of 5.72. Similar searches with thededuced amino-acid sequence of f ull-length CalliphoraAFP, ... according to the following scheme: 3 · 10 min with 4 · NaCl/Cit; 2 · 10minwith2· NaCl/Cit; 1 0 min with 0.1 · NaCl/Cit; 10 min with 0.05 · NaCl/Cit; 5 min with NaCl/Tris. After incubation for 30 m ... encoded a protein with 93% identity in the deduced amino-acid sequence with the A FP of S. peregrina (GenBank accession numberBAA99282). Because of the high sequence identity with the Sarcophaga...
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