insight into the molecular mechanism of translesion dnasynthesis in human cells using probes with chemically defined dna lesions
Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
Ngày tải lên :
07/03/2014, 15:20
... exotoxin, all of the residues of the catalytic mechanism are present in the neighborhood of the catalytic site, but they are not ordered correctly in the exotoxin alone CaM binding folds parts of the ... CaM-binding domain probed by W242 W242 dominates the intrinsic fluorescence emission of the protein [20] It is situated in the middle of the 72-amino-acid segment responsible for 90% of the AC–CaM binding ... in the CaM-binding domain Most of the crucial amino-acid residues responsible for catalysis and ATP binding are, however, conserved in both proteins [23] With respect to the 3D structure of the...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
Ngày tải lên :
21/02/2014, 01:21
... coordination interaction with one of the manganese ions This, together with the fact that the positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains ... arginases, the agmatinase loop was shorter when compared with the same region of the arginases (Fig 4) Since the arginase loop contains residues that interacts with the a-carboxylate group of the ... deletions in the agmatinase sequence were modelled using the DGLOOP set of options in WHATIF; the whole loops and the two connecting amino acids at the beginning and the end, were mutated to glycines...
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Báo cáo y học: "Insight into the molecular requirements for pathogenicity of Fusarium oxysporum" pdf
Ngày tải lên :
14/08/2014, 21:20
... modes of T -DNA integration the chromosomal DNA was cut with BglII, which does not cut within the T -DNA, or BamHI, which cuts once in the T -DNA, and hybridized with five different probes, notably the ... analyzed in the same way; data not shown) In 22 of the mutants in which a double T -DNA integration had occurred, the two T -DNA copies had integrated in close proximity of each other or exactly at the ... leading to inverted repeats Only in six mutants with two insertions had the TDNAs integrated in two different chromosomal locations Finally, based on the results obtained with the LB and RB probes, ...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
Ngày tải lên :
19/02/2014, 16:20
... favourable for binding of Glc +2 of the a-(1fi4)-linked glucose units of pullulan (Fig 4A) IPU is an anomer-inverting enzyme Comparison of the active sites of the model of IPU and Dex49A The active ... of Trp402 of IPU, is reportedly located in the vicinity of the active site and could form a binding site for a glucosyl unit in subsite )1 (Fig 4B) Together with the observations from the site-directed ... composed of residues 25–540 The overall structure of the model of IPU and the mutated residues in this study are shown in Fig To elucidate the mechanism of the substrate recognition of IPU, two...
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Báo cáo khoa học: Transthyretin and familial amyloidotic polyneuropathy Recent progress in understanding the molecular mechanism of neurodegeneration pdf
Ngày tải lên :
07/03/2014, 10:20
... resulting from the occupancy of the first binding site [68] TTR is also involved in the transportation of retinol by forming a complex with the smaller retinolbinding protein (RBP) [69,70] The TTR–RBP–retinol ... these low -molecular- mass diffusible species can bind to lipid membranes In the model, binding to the lipid membrane disrupts the structure of the lipid rafts, thereby inducing changes in the membrane, ... interactions between the A–B loop of one monomer and the ˚ H strand of the opposite dimer, creating a 50 A central channel that contains the two binding sites for thyroxine [81] The four binding sites for...
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Báo cáo khoa học: Insight into the phosphodiesterase mechanism from combined QM ⁄ MM free energy simulations pot
Ngày tải lên :
14/03/2014, 23:20
... structure of a small fragment of PDE4D was reported in 1996 [5,13], key insights into the understanding of the catalytic active site of PDEs were obtained following the determination of the crystal ... residues (Fig 1) The hydrogen atoms of H2O2 form hydrogen bonds with the side chain of Glu304 and the backbone of Thr345 Interestingly, the side chain of Thr345, together with the backbone of His274, ... interactions of the phosphate group of AMP with the two metal ions, the adenine group and ribosyl ring of AMP are also bound subtly with the active site The pentose ring has a conguration of O3Â forming...
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Báo cáo y học: "The molecular mechanism of osteoclastogenesis in rheumatoid arthritis" pptx
Ngày tải lên :
09/08/2014, 06:22
... examined whether sIL-6R and IL-6 are involved in joint destruction in RA patients [13] The number of osteoclast-like multinucleated cells found in the synovial tissues derived from the knee joint ... which interferes with the RANKL–RANK signaling pathway In that study, osteoclast formation was strongly inhibited in the coculture of activated T cells and bone marrow cells in the presence of RANKL ... model of arthritis, when compared with the control mice [39] To elucidate the direct effect of human T cells in inducing osteoclastogenesis in RA, we conducted coculture experiments of activated human...
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báo cáo khoa học: " Insight into the early steps of root hair formation revealed by the procuste1 cellulose synthase mutant of Arabidopsis thaliana" doc
Ngày tải lên :
12/08/2014, 05:20
... chains of β-1,4-linked glucosyl residues which are assembled into microfibrils The arrangement of the microfibrils in the cell wall influences the manner in which cells expand A number of mutants ... shows that the bulging of epidermal cells is associated with their radial position with respect to the underlying cortical cells and that it may be linked with the process of hair initiation ... hairs by wild-type seedlings can be inhibited either by blocking the ethylene receptor using silver ions [25] or via inhibition of ethylene synthesis using aminoethoxyvinyl-Glycine (AVG) [6,26,33]...
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NVESTIGATING THE MOLECULAR MECHANISM OF PHOSPHOLAMBAN REGULATION OF THE Ca2+-PUMP OF CARDIAC SARCOPLASMIC RETICULUM
Ngày tải lên :
24/08/2014, 11:06
... SERCA2a, increasing the apparent Ca2+ affinity of the enzyme and increasing the rate of Ca2+ uptake into the SR (2, 3) However, although all three of these Ca2+ handling pathways contribute to the ... point in the mechanism of PLB action HYPOTHESIS 1: SERCA2a WITH PLB BOUND IS CATALYTICALLY INACTIVE There are two schools of thought with respect to PLB binding interactions with SERCA2a In the ... therapeutic treatment of heart failure, underscoring the necessity of elucidating the molecular mechanism of PLB action D THE MECHANISM OF Ca2+ TRANSPORT BY SERCA2a SERCA is a large protein of...
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The Molecular Mechanism of Break Induced Replication
Ngày tải lên :
24/08/2014, 13:24
... pre-‐existing nicks/ lesions in the DNA backbone (Aguilera, 2005; Kuzminov, 1997), by excision of DNA transposable elements, due to inability of topoisomerases in completing their ... The repair of DNA DSBs by HR requires that the ends of the broken DNA be available in the form of 3’ single stranded DNA (ssDNA) tails or overhangs These form substrates ... mechanisms that preserve genomic integrity in yeast are the same ones that go haywire in cancer Therefore understanding the underlying molecular mechanisms of DNA...
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UNDERSTANDING THE MOLECULAR MECHANISM OF CENTRINS IN TRYPANOSOMA BRUCEI
Ngày tải lên :
12/10/2015, 17:35
... in thesis writing Her innovative insight and logical way of thinking have been of great value for me, under the influence of which my knowledge was enhanced and the depth of my scientific thinking ... database screening for T brucei proteins containing centrin binding motif initially characterized in Sfi1p have been used Centrins are proteins functioning through interaction with other proteins (see ... to the nucleus cycle, with kinetoplast S-phase initiating prior to the onset of nuclear S-phase and the division of kinetoplast DNA having completed before the onset of nuclear mitosis According...
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Investigating the molecular mechanism of ERp29 regulated cell cycle arrest in breast cancer
Ngày tải lên :
12/10/2015, 17:35
... MDA-MB-231 cells, in contrast to the accumulation of cyclinD1 inside the cell nuclei, in ERp29-silenced MCF7 cells Using immunofluorescence imaging, the translocation of cyclinD1 into the cytoplasm ... behind the breastbone Stage IIIB A tumor of any size that has grown into the chest wall or the skin of the breast It may be associated with swelling of the breast or with nodules (lumps) in the ... radiation therapy are types of local therapy They remove or destroy cancer cells within the breast Hormone therapy, chemotherapy, and targeted therapy are types of systemic therapy The drug enters the...
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An investigation into the linguistic features of interogative sentences in english and vietnamese communication
Ngày tải lên :
14/05/2016, 18:40
... Representatives: Commit the speaker to something being b) The Illocutionary Act: is the making of an act in uttering a sentence, by virtue of the conventional force as sociated with it (or with the case such ... 2.5 Invitation 0 Greeting 0 200 100 l) Inviting m) Showing Greeing 4.2.2.2 The Frequency of Pragmatic Characteristics of ISs in Vietnamese Table 4.4 The Frequency of Pragmatic Characteristics of ... ISs Table 4.2 The Percentage of Syntactic Characteristics of ISs in Combining with the word “ñâu” Vietnamese (89) “Tuy chị có ngủ ñược ñâu” Category [120, p.37] Combining with the word “bao giờ”...
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Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
Ngày tải lên :
03/11/2012, 11:17
... expression of claudin-1 in the cytoplasm, with some nuclear expression of claudin-1 B) Treatment of these cells with PKA inhibitor results in nuclear translocation of claudin-1 in as little as 15 minutes, ... transfected with CLDN1 under the control of a nuclear localization signal (CLDN1-NLS), there is increased expression of claudin-1 in the nucleus, but there are still large amounts of claudin-1 in the ... localization of claudin-1 Nuclear claudin-1 does not increase melanoma cell motility We have previously shown that increasing the levels of CLDN-1 increases the invasion of melanoma cells (4) To determine...
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Báo cáo y học: "Defining the chromatin signature of inducible genes in T cells" pps
Ngày tải lên :
09/08/2014, 20:20
... to the original GCOS/ MAS5 normalization used in the original paper The gene coordinates for the human orthologs of the mouse genes were obtained using BIOMART R was used to count the number of ... overlapped with the promoter regions (1 kb to +1 kb of the annotated TSSs) of the human orthologs of the mouse genes The basal expression level bins were adjusted using expression profiling data ... compare Rho with Gapdh) it is important to compare the level of this mark within the transcribed region and the promoter region of any one gene to gauge the level of enrichment within the gene The three...
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Báo cáo y học: " Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity" pot
Ngày tải lên :
14/08/2014, 08:20
... Table [20]), including members of the integrin family, such as integrin alpha V (ITGAV), integrin beta (ITGB4), and integrin beta (ITGB6) Enzymes involved in the degradation of glycosaminoglycans ... death-associated protein (DAP), the deathassociated protein kinase (DAPK2), and the serine/threonine kinase 17a (STK17A), a member of the DAP kinaserelated apoptosis-inducing protein kinase family, as ... view of interacting genes and related functions in the adipose tissue Additionally, we also observed in the cell culture study that a panel of inflammatory cytokines, including interleukins and their...
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Báo cáo y học: "Novel insights into the relationships between dendritic cell subsets in human and mouse revealed by genome-wide expression profiling" ppt
Ngày tải lên :
14/08/2014, 08:20
... involved in the biology of these cells, to the best of our knowledge, such as the E430004N04Rik expressed sequence tag in T cells, the Klhl14 gene in B cells, or the Osbpl5 gene in NK cells In ... [31] All of the human samples were done in independent triplicates Information regarding the original sources and the public accessibility of the datasets analyzed in the paper are given in Table ... on the one hand versus mCD11b and hBDCA1 cDCs on the other hand Our finding that LN-DCs constitute a distinct entity within immune cells raises the question of whether these cells form a distinct...
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The spatial distribution of dust sources in iraq by using satellite images
Ngày tải lên :
09/09/2015, 10:17
... observed in Ninewah city, it shows that the general trend of temperatures increase, heat waves are increasing, the number of hot days increase in the summer, decreases the amount of rainfall, ... that the northwestern region and the western region are the sources of the emission of dust particles, these reigns represented in the governorates of Al-Anbar and Ninwah Through the study of climate ... rainfall, especially during the last two decades, bad distribution of rainfall during the rainy season, late rainy season and increases the frequency of drought cycles and severity in the region 2008...
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Exploration of the functional significance of mig 2 in human cancer cell susceptibility to cytotoxic agents and cell growth control a pilot study
Ngày tải lên :
05/10/2015, 22:15
... activating different intracellular signaling and adaptor proteins ECM binding initiates clustering of integrins in the plane of the membrane and reorganization of the actin cytoskeleton, which further ... regulated in patients with Kindler syndrome, an autosomal recessive skin disorder They therefore defined a family of kindlin-related proteins namely Kindlin-1, Kindlin-2 (mig-2) and Kindlin-3 (Siegel ... as a new binding partner for integrinlinked kinase (ILK), which is also an adaptor within integrin-associated cytoskeletal complexes implicated in integrin-mediated signaling (Mackinnon et al.,...
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Báo cáo y học: "Genome-wide assessment of imprinted expression in human cells." potx
Ngày tải lên :
09/08/2014, 22:24
... heritable in LCLs but imprinted in fibroblasts, further study of the two cell lines could help identify some of the factors involved in the mechanism of imprinting Interestingly, another study ... growth and imprinting, they have likely evolved more than once [7] The debate around theories of imprinting parallels the intense investigation of the mechanisms that maintain imprinting Monoallelic ... DNA according to the manufacturer’s protocol in order to verify demethylation of the DNA upon AZA treatment In the first step, μg of DNA was mixed with His-MBD2b protein, along with the binding...
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