inferior surface of the bony skull

Báo cáo hóa học: " Mapping of immunogenic and protein-interacting regions at the surface of the seven-bladed β-propeller domain of the HIV-1 cellular interactor EED" pptx

Ngày tải lên : 20/06/2014, 01:20

... periphery of the β-propeller (Fig 2D) Of note, the upper face of the β-propeller was narrower in surface, compared to its lower face However, there was some ambiguity in the determination of the IN ... towards the lower face of the βpropeller (Fig 3) The absence of overlapping of the MA and IN binding sites and the possible occurrence of ternary complex involving EED, MA and IN raised the issue of ... of the binding sites of HIV-1 MA and IN [15,16] Several immunoreactive regions coincided with the MA, IN and EZH2 binding sites, confirming the accessibility of these regions at the surface of...

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378
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Tài liệu Conditions of the surface water and ground water resources in the rural area of the Mekong Delta, Vietnam pptx

Ngày tải lên : 16/01/2014, 17:20

... following the Vietnamese standards for ground water TCVN 5944 –1995 (see table 3) The results of the analyses of the surface water samples were compared to the Vietnamese standards for the use of surface ... from the irrigation ditches of the rice fields At both study sites the values for the pH, BOD, NH4 and phosphate are conform to the requirements of the Vietnamese standards for the use of surface ... where an interaction of the tidal regimes of the South Chinese Sea and of the Gulf of Thailand eliminate each other Here, the tidal amplitude is less than 10 cm Because of its lower elevation...

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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx

Ngày tải lên : 21/02/2014, 03:20

... USA) DNA quanti®cation The DNA content of the cell layer at the end of incubation was quanti®ed by the method of West et al [31] After solubilization of the cell layer in M NaOH and subsequent ... implication of cAMP increase, the second messenger of FSH, in the bFGF regulation was evaluated by addition to the culture medium of either mM dbcAMP or 10 lgámL)1 cholera toxin Their addition ... testicular development, the physiology of Sertoli cells is modi®ed The cell proliferation decreases and ceases allowing the establishment of the hematotesticular barrier around the 20th day postpartum...

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Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx

Ngày tải lên : 21/02/2014, 03:20

... wild-type ALP I On the other hand, the Tm of D266N/Y269A-ALP I increased by only °C The denaturation temperature indicates the stability of the structure of the protein The difference of the increments ... surrounding residues of the depression, and the side chain of the mutated residue might restrain the mobility of the N-terminal region on the surface by interaction with the main chain of Lys26 by van ... the substitutions did not inﬂuence the activities or speciﬁcity of the mutant enzymes The N-terminal region of ALP I was also on the surface The side chains of Ile10 and Tyr11 were close to the...

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Multilevel modeling of the influence of surface transport peculiarities on growth, shaping, and doping of si nanowires

Ngày tải lên : 16/03/2014, 15:18

... diffusion of silicon atoms from the surface to the catalyst/NW interface Here, h is the height of the NW and O is the atomic volume of a silicon atom in a silicon lattice, the factor is the ratio of the ... like manner, the output ﬂux is the number of adatoms leaving the given region of the surface (due to the surface transport), per one MC time step and per unit of the boundary length The surface transport ... equations describing the growth of NWs The second two MC experiments reproduce a fragment of a Fig The MC simulations of the kinetics for spreading of the mobile atoms across the surface in a two-component...

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514
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Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx

Ngày tải lên : 17/03/2014, 03:20

... the importance of these amino acids in the interaction between the 4-kDa peptide and the 43-kDa protein The secondary structures of these two fragments, as revealed from NMR spectroscopy of the ... binding to the 43-kDa protein The topology of the sidechains of Ile25, Val29, Phe31 and Ile33 in the 4-kDa peptide was similar to that of the active area in insulin (Fig 7F) If the mechanism of the ... determine the potential association of the 43-kDa protein, as the receptor of the physiologically active peptide usually forms an oligomer to activate the function of the receptor [12] When the 43-kDa...

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Báo cáo khoa học: Cofactor-independent oxygenation reactions catalyzed by soluble methane monooxygenase at the surface of a modiﬁed gold electrode pot

Ngày tải lên : 17/03/2014, 09:20

... Detection of the products of substrate oxygenation by the adsorbed MMOH was achieved by holding the potential at )0.5 V for 30 in the presence of substrate and then removing the liquid (50 lL) from the ... computer fitted with GPES software The surface of the 4-mm diameter gold electrode (Oxford Electrodes) was modified by cycling the electrode at reducing potentials in the presence of the hexapeptide, as ... synthesized using a Pioneer Peptide synthesiser (Applied Biosystems) and purified by means of HPLC using a Jupiter RPC18 column means of a stream of air from an electric fan The effect of the...

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Báo cáo khoa học: Creation of a new eye lens crystallin (Gambeta) through structure-guided mutagenic grafting of the surface of bB2 crystallin onto the hydrophobic core of cB crystallin pot

Ngày tải lên : 23/03/2014, 04:21

... residues occur upon the surfaces of bB2 and cB Furthermore, the bottom part of Fig shows a schematic representation of the transplantation This part of the ﬁgure emphasizes the conceptual point ... encoding the residues L and N at the N-terminal end of the truncated bB2, and the residues Q and W at the C-terminal end of truncated bB2, because these four residues are present in the structure of ... unfolding of the two domains, which could be related to the fact that the protein shows non-cooperativity of unfolding To explore the reversibility of the unfolding transitions, far-UV CD spectra of...

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Báo cáo khóa học: CD38 is expressed as noncovalently associated homodimers on the surface of murine B lymphocytes doc

Ngày tải lên : 23/03/2014, 12:20

... 3B), these data suggest that these domains are not obligate for the stabilization of the dimers, but rather must contribute to the overall stability of the dimers Crystallographic analysis of the ... folding of proteins or inappropriate assembly of multimeric protein complexes can inﬂuence the surface and overall expression of these proteins in cells and can also alter the half-life of the misfolded ... and surface half-life, it was also possible that these mutations would affect the enzyme activity of the proteins To test the enzyme activity of the CD38 mutants, membrane homogenates from the...

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448
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Báo cáo khoa học: The A-subunit of surface-bound Shiga toxin stimulates clathrin-dependent uptake of the toxin ppt

Ngày tải lên : 23/03/2014, 15:20

... for the stimulation of endocytosis However, the requirement for the A-subunit could be mediated via an effect of the B-moiety This illustrates the importance of detailed studies of the role of the ... toxin was cleaved off in some of the wells by the cell-impermeable reducing agent MESNa, thereby preventing detection of the still surface- bound toxins [38] In the experiments, the cells were washed ... 7.0 software Estimation of cell surface- bound toxin concentration To be able to compare the local concentration of Stx on the cell surface and in the medium, the labeled toxin bound to the cells...

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Báo cáo khoa học: Characterization of surface n -alkanes and fatty acids of the epiphytic lichen Xanthoria parietina, its photobiont a green alga Trebouxia sp., and its mycobiont, from the Jerusalem hills pot

Ngày tải lên : 23/03/2014, 17:21

... amounts in the ﬁrst sample of the two Cetraria species Also observed in the fraction of hydrocarbons of the second sample of S ceratites were a series of anteiso-alkanes Anteiso-C26 was the most ... 14 species of Argentinian lichens for the presence of alkanes, and found that the main ones were C27, C29 and C31 The alkanediene C17H32 and normal alkanes in the C9–C17 range, with the C17 alkadiene ... made up of a relatively high amount of n-alkanes ranging from C18 to C34 The main compounds detected in the Abu Ghosh Valley and other places near the Jerusalem hills were C24, C25, and C26 The total...

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Báo cáo khoa học: Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64 ppt

Ngày tải lên : 23/03/2014, 21:21

... · · · · · 107 107 107 107 107 Presentation of the ELDKWA on surface of infected cells The presentation of the inserted epitope ELDKWA on the surface of infected cells was determined by FACS analysis ... concluded from FACS histograms the amount of ELDKWA on the surface of infected cells only varied slightly approving the stable insertion of the ELDKWA peptide into the native envelope protein gp64 ... demonstrating the disruption of the epitope of this mouse mAb and the absence of any wild-type gp64 (Fig 3B) Expression of recombinant gp64 in infected insect cells The expression levels of recombinant...

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338
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Báo cáo khoa học: Role of the surface charges D72 and K8 in the function and structural stability of the cytochrome c6 from Nostoc sp. PCC 7119 potx

Ngày tải lên : 30/03/2014, 20:20

... neutralization of the effect of the mutation D72K on the protein’s global charge was not sufﬁcient to fully reverse the negative effect on the stability of cyt c6 The apparent stability of the mutants ... signiﬁcance of the charge in amino acid position for the formation of a productive encounter complex during the electron transfer from cyt c6 to PS I K8 is located at the edge of the contact surface of ... mutation of surface residues located far from the heme moiety Thus, the thermodynamic driving force for the reaction of cyt c6 with PS I was essentially unaltered by the introduction of the mutations...

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469
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Báo cáo sinh học: " The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient c" pptx

Ngày tải lên : 18/06/2014, 18:20

... Site-Directed Mutagenesis Kit of Stratagene) in order to disrupt the putative transmembrane helix of each of the three domains of the E5 protein [34-36] without altering the length of the protein All PCR-generated ... complex of HPV16 E5, calnexin, and the heavy chain of HLA-I molecules The formation of this complex depends on the presence of the first predicted transmembrane domain of HPV16 E5 Since the dimer ... showing the expression levels of the codon-optimised E5 gene (left panel) and of the viral E5 gene (right panel) Note the differences in the immunoreactivity signals despite the higher amount of total...

15
411
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Báo cáo hóa học: " Influence of the training set on the accuracy of surface EMG classification in dynamic contractions for the control of multifunction prostheses" potx

Ngày tải lên : 19/06/2014, 08:20

... trade-off between decision delay and accuracy using the majority vote [12] The final decision was taken by majority vote on the most recent results The response time is the sum of the length of the ... reducing the confusion of the rest class with other classes Figure displays the error rate of each pair of feature set and classifier when the training was exclusively performed on the static part of ... from all the others Including the dynamic portion in the training set increases substantially the number of windows available for each class, and so the unbalance between the sizes of the two...

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báo cáo hóa học:" Unsaturated phosphatidylcholines lining on the surface of cartilage and its possible physiological roles" potx

Ngày tải lên : 20/06/2014, 00:20

... analysis The total amount of PC was then worked out by adding the amounts of individual PC species together In our study the total amount of PC species was < 20 µg The relative percentages of each of ... until no further movement of fluid was seen At the end of each experiment, the final pressure difference, ∆P, was recorded as the difference in the heights between the two fluid columns The mean ... procedure, soft facial tissues soaked with Folch solvent were used to wipe SAPL off from the articular surface The contact time between the solvent and articular surface at each of these selected...

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432
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Báo cáo hóa học: " The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient cells" ppt

Ngày tải lên : 20/06/2014, 01:20

... Site-Directed Mutagenesis Kit of Stratagene) in order to disrupt the putative transmembrane helix of each of the three domains of the E5 protein [34-36] without altering the length of the protein All PCR-generated ... complex of HPV16 E5, calnexin, and the heavy chain of HLA-I molecules The formation of this complex depends on the presence of the first predicted transmembrane domain of HPV16 E5 Since the dimer ... showing the expression levels of the codon-optimised E5 gene (left panel) and of the viral E5 gene (right panel) Note the differences in the immunoreactivity signals despite the higher amount of total...

15
390
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Báo cáo hóa học: " The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein GP-C" ppt

Ngày tải lên : 20/06/2014, 01:20

... we determined the effect of the individual N-glycans on the maturation cleavage by analysing the presence of the cleaved subunit GP-2 Disruption of the N-glycosylation sites within the GP-1 mutants ... participated in the analysis of the results and drafted the manuscript OL helped to draft the manuscript and revised it critically WG designed the study, participated in the analysis of the results ... cleavage of the glycoprotein was observed within the mutants S367A and S375A (Fig 2B, lanes and 3, lower panel) Due to the high resolution of the acrylamid gel electrophoresis, the GP-2 subunit of Schematic...

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363
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Báo cáo toán học: " Spin-related tunneling through a nanostructured electric-magnetic barrier on the surface of a topological insulator" potx

Ngày tải lên : 20/06/2014, 20:20

... polarized along the direction of the vector (π × z), the magnetic field bends the trajectory of the electrons, resulting in a rotation of the spin of the transmitted electrons The reflected electrons ... Our results demonstrate that the magnetic field of the magnetic barrier bends the trajectory of the electrons, and therefore rotate the spin Theory First we focus on the electron transmission through ... properties In the surface, Hamiltonian of the 3D TI σ acts on the real spin of the charge carriers, while for graphene it stands for the pseudo spin, i.e., the A and B sublattices of graphene Hence,...

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Báo cáo hóa học: "Effect of the carbon nanotube surface characteristics on the conductivity and dielectric constant of carbon nanotube/poly(vinylidene fluoride) composites" doc

Ngày tải lên : 21/06/2014, 04:20

... CNTs The reduction of the electrical surface conductivity of the CNT due to the oxidation process, together with an increase of the surface area and defect formation, is at the origin of the observed ... that the incorporation of the CNT in the PVDF matrix but leads to a gradual increase of the dielectric constant (ε’) as the amount of the filler is increased (Figure 4b) The increase of the ε’ ... reduction of the surface conductivity of the CNTs due to the oxidation process [8], and is similar for all the functionalized composites Further, the increase of surface area due to the functionalization...

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