impact of the hiv 1 opsonization pattern on dc function

Báo cáo y học: "Impact of the Kenya post-election crisis on clinic attendance and medication adherence for HIV-infected children in western Kenya"

... (0. 61 1. 09) 0.87 (0. 61 1. 23) On ART 0.44 (0. 31 0.62) 0.58 (0.43–0.78) Orphan 0.82 (0. 61 1. 10) 1. 03 (0.67 1. 55)

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Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot

Danh mục: Báo cáo khoa học

... 61 60 52 61 14 12 0.003 0.0 01 > 10 0 (58%) > 10 0 (10 0%) > 10 0 (10 0%) > 10 0 (98%) 21 16 > 10 0 (10 0%) 16 ND 68 10 0 10 0 35 93 92 64 28 0.68 0.2 > 10 0 (10 0%) > 10 0 (10 0%) > 10 0 (10 0%) ... Asn103 Tyr1 81 VAL108A VAL106A LEU234A ASN103A TYR188A Tyr1 81 D H O O O K126 Asp 110 VAL108A O O O TYR188A VAL108A O Asp 110 O O O VAL106A Asp186 TYR188A Trp229 Asp186 PHE227A NNRTIbp Asp185 K126 ... 61 K-53 > 10 0 (10 0%) > 10 0 K-54 39 60 KNA-53 21 52 K -12 6 10 0 61 K- 61 > 10 0 ( 91% ) 14 K -11 1 > 10 0 (80%) > 10 0 (10 0%) K-49 13 12 EFV > 10 0.003 0.0 01 a Compound concentration required to...

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Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx

Danh mục: Báo cáo khoa học

... presence of the excess of the Tat peptide, in keeping with separate internalization pathways Internalization of the Tat±GFP fusion construct in these conditions was poorly ef®cient in the A-745 clone ... treatment on CHO K1 cells [ 21] Likewise, heparinase treatment of HeLa cells completely inhibits the uptake of the Tat±GFP fusion protein (Fig 6A, dotted line) On the contrary, the internalization of the ... analysis, the concentration of the recombinant protein was increased to lgámL )1 The internalization of the GST±Tat construct was monitored by immunodetection as described previously [ 21] After...

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Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx

Danh mục: Báo cáo khoa học

... of 12 .3 s )1 (range 9 .1 14 .0); the second a-helix presented a mean of 13 .2 s )1 (range 11 .9 14 .2); the third a-helix presented a mean of 11 .3 s )1 (range 8 .19 – 13 .5); and, ﬁnally, amino acids in the ... constant of the free space, cN and cH are the gyromagnetic ratios of 15 N ()2. 71 · 10 7 radÆs )1 T )1) and 1H (2.68 · 10 8 radÆs )1 T )1) , h is the Planck constant, xN is the Larmor frequency of the 15 N, ... motions (i.e the internal motions of the bond vectors are independent of the overall rotational movement of the molecule) Furthermore, the internal motions of each bond vector are independent of...

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Báo cáo khoa học: Kinetic study of the HIV)1 DNA 3¢-end processing Single-turnover property of integrase docx

Danh mục: Báo cáo khoa học

... (19 96) Linear diffusion of the restriction endonuclease EcoRV on DNA is essential for the in vivo function of the enzyme EMBO J 15 , 510 4 511 1 40 Harper AL, Skinner LM, Sudol M & Katzman M (20 01) ... inactivation (T1 2, IN alone ẳ 11 1 min; T1 2, IN+DNA ẳ 15 4 min; T1 2, IN+Mg ẳ 222 min) FEBS Journal 273 (2006) 11 3 711 51 ê 2006 The Authors Journal compilation ê 2006 FEBS M Smolov et al DNA allows the ... preincubation of IN with 11 44 [Product] (nM) 0 .12 0.08 T1/2 = 11 1 15 0.04 0.00 200 400 600 800 10 00 Time (min) B [Product] (nM) Stability of IN during the 3Â-processing reaction A 0 .12 0.08 T1/2 =...

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Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/triﬂuoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot

Danh mục: Báo cáo khoa học

... panel: Backbone superimposition of 10 selected structures of (1 96)Vpr, performed on the (15 –33) (38–49) (54–74) a helices The average rmsd of the back˚ bone for the (17 –83) region is 5.0 A Helices ... that maintain the relative orientation of the ﬁrst and second helices, on one side, and the second and third helices on the other side of the molecule It is reasonable to suggest that these local ... This is one of the main reasons why the aim of this study was to determine the solution structure of the intact monomeric form of this protein One of the main problems encountered with (1 96)Vpr,...

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Báo cáo sinh học: " Viroporin potential of the lentivirus lytic peptide (LLP) domains of the HIV-1 gp41 protein" docx

Danh mục: Hóa học - Dầu khí

... Zasloff M, Stanley EF: Magainin 2, a natural antibiotic from frog skin, Page 12 of 14 (page number not for citation purposes) Virology Journal 2007, 4 :12 3 10 11 12 13 14 15 16 17 18 19 20 21 22 ... http://www.virologyj.com/content/4 /1/ 123 osmolysis The ability of UV-inactivated HIV to cause single cell balloon degeneration in the absence of replication argues for the involvement of a virion component, possibly the ... virion Since the LLP domains are also present in the context of the virion, it is possible that they would have an effect at this stage of the HIV replication cycle There is at least one report of...

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Báo cáo hóa học: " Viroporin potential of the lentivirus lytic peptide (LLP) domains of the HIV-1 gp41 protein" docx

Danh mục: Hóa học - Dầu khí

... Zasloff M, Stanley EF: Magainin 2, a natural antibiotic from frog skin, Page 12 of 14 (page number not for citation purposes) Virology Journal 2007, 4 :12 3 10 11 12 13 14 15 16 17 18 19 20 21 22 ... http://www.virologyj.com/content/4 /1/ 123 osmolysis The ability of UV-inactivated HIV to cause single cell balloon degeneration in the absence of replication argues for the involvement of a virion component, possibly the ... virion Since the LLP domains are also present in the context of the virion, it is possible that they would have an effect at this stage of the HIV replication cycle There is at least one report of...

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Báo cáo hóa học: " Mapping of immunogenic and protein-interacting regions at the surface of the seven-bladed β-propeller domain of the HIV-1 cellular interactor EED" pptx

Danh mục: Hóa học - Dầu khí

... Biol 19 97, 17 :6663-6672 Page of (page number not for citation purposes) Virology Journal 2008, 5:32 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Schumacher A, Faust C, Magnuson T: Positional cloning ... position 294–309 on the linear sequence of EED [15 ] The newly established conformation of this http://www.virologyj.com/content/5 /1/ 32 region implied that the region of interaction with the MA protein ... regions at the surface of EED According to the EED 3D-model, the domain of interaction with the HIV- 1 MA protein would be localised on the lateral face of the β-propeller, and be comprised of...

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Báo cáo y học: " Reduction of the HIV-1 reservoir in resting CD4+ T-lymphocytes by high dosage intravenous immunoglobulin treatment: a proof-of-concept study" pot

Danh mục: Báo cáo khoa học

... (Figure 4) Discussion The latency of HIV- 1 in resting CD4+ T-lymphocytes constitutes a major obstacle for the eradication of the virus in patients on ART The decay rate of the latent reservoir ... and involve modulation of expression and function of Fc receptors; interference with complement activation and the cytokine network; effects on the activation and function of lymphocytes, dendritic ... (projects K2007-56X-20345 01- 3 and 2007-7092) http://www.aidsrestherapy.com/content/6 /1/ 15 17 18 19 References 10 11 12 13 14 15 16 Chun TW, Carruth L, Finzi D, Shen X, DiGiuseppe JA, Taylor H, Hermankova...

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Báo cáo y học: "Characterization of the HIV-1 integrase chromatin- and LEDGF/p75-binding abilities by mutagenic analysis within the catalytic core domain of integrase" ppt

Danh mục: Báo cáo khoa học

... Interaction with LEDGF/p75 +++ D64/D 116 99.4/99.7 DD64, 11 6EA +++ ++ K136 31. 3 K136A +++ ++ K159 99.5 K159P - +/- V165 93.5 V165A - - E170 99.6 H171A +++ - H1 71 98.5 L172A +++ - L172 K173 99.4 ... Journal 2 010 , 7:68 http://www.virologyj.com/content/7 /1/ 68 Page of 14 highlighted the importance of E170A, H171A, LK172,3AA for LEDGF/p75 interaction [11 ,12 ,26] The mutants K136, K159 were also ... EH170,1AA EK170,3AA ++ +++ +++ V176 99.4 HL1 71, 2AA - - A179 99.8 HK1 71, 3AA ++ ++ I182 98.0 V176A - - F185 99.4 A179P - - KR186,7 99.7/99.0 A179I - NA I200 98.3 I182A + ++ I203 96.8 F185A KR186,7AA...

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Báo cáo y học: "In vitro nuclear interactome of the HIV-1 Tat protein" pps

Danh mục: Báo cáo khoa học

... Characterization of a protein complex containing spliceosomal pro- http://www.retrovirology.com/content/6 /1/ 47 93 94 95 96 97 98 99 10 0 10 1 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9 11 0 11 1 11 2 11 3 teins SAPs 49, 13 0, ... purposes) Retrovirology 2009, 6:47 15 6 15 7 15 8 15 9 16 0 16 1 16 2 16 3 16 4 16 5 16 6 16 7 16 8 16 9 17 0 17 1 17 2 17 3 17 4 17 5 isoforms of the mRNA binding protein AUF1 (hnRNP D) Biol Chem 2003, 384:25-37 ... 70.25 18 .9 18 .30 8.20 12 .8 26.60 16 .6 16 .7 12 5.4 5.6 5 .1 11. 1 23.00 38.8 11 .6 58 .1 18.60 6.2 24.2 11 .5 18 4529.4 40666.5 13 2649.7 88420.5 44669.4 619 36.8 35902.7 217 042.6 615 20.8 83 419 .5 13 3 810 .7...

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Báo cáo y học: "What does the structure-function relationship of the HIV-1 Tat protein teach us about developing an AIDS vaccine?" ppsx

Danh mục: Báo cáo khoa học

... Tat-vacci- Page 12 of 13 (page number not for citation purposes) Retrovirology 2009, 6:50 10 3 10 4 10 5 10 6 10 7 10 8 10 9 11 0 11 1 11 2 11 3 11 4 11 5 11 6 11 7 11 8 nated macaques not control simian immunodeficiency ... [49,50,95 ,10 8 ,11 0, 11 1] It is notable that with a sera dilution of 1: 1000, Tat Bru is recognized by only 30% of the RP patients in Europe [95] and only 10 to 14 % of RP patients in Africa [11 1] This ... representative of the five main HIV- 1 subtypes The sequence length of Tat is variable and ranges from 86 to 10 1 residues as a function of the second exon A viable strain having only the first exon of Tat...

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Báo cáo y học: " Changes in the accessibility of the HIV-1 Integrase C-terminus in the presence of cellular proteins" doc

Danh mục: Báo cáo khoa học

... 278:372-3 81 Benkhelifa-Ziyyat et al Retrovirology 2 010 , 7:27 http://www.retrovirology.com/content/7 /1/ 27 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Kalpana GV, Marmon S, Wang ... sequences In the context of HIV- 1NLX, the insertion of the tag introduced a stop codon in the overlapping vif gene Although vif activity is irrelevant in the context of SupT1 and MAGI cells, the modification ... MH532 primers [17 ] The absence of amplification in the presence of AZT indicates that only neosynthesized DNA was detected Page of 10 masking of the IN C-terminal tag Transductions of HEK 293 cells...

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Báo cáo y học: " Role of the C-terminal domain of the HIV-1 glycoprotein in cell-to-cell viral transmission between T lymphocytes" potx

Danh mục: Báo cáo khoa học

... 10 10 10 10 10 10 10 10 GFP 10 10 10 10 10 10 10 10 10 10 GFP GFP 10 np / Tr 712 , high Cell number 10 0% 10 10 p24 D 10 np / Fus-, high 80% 10 10 10 10 p24 10 np / Wt, low 0% 10 10 10 10 GFP 10 10 ... 10 10 p24 10 10 np / Tr 712 , low 10 0% 10 10 10 p24 10 15 % 10 10 10 10 p24 np / high p / Tr 712 , low 10 0% 10 10 10 10 Dye 10 10 10 10 10 11 0% 10 3% 10 Dye 10 10 % cell-to-cell transmission p24 10 10 ... http://www.retrovirology.com/content/7 /1/ 43 A H9 target cells H9 donors 10 Page of 11 MT- donors MT- target cells p24 10 10 10 10 10 10 10 10 10 10 10 10 10 4 10 10 B 10 10 np / Wt, high 10 10 7% 10 np / Tr 712 , low 10 0% 10 ...

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Báo cáo y học: "Evolution of the HIV-1 nef gene in HLA-B*57 Positive Elite Suppressors" pdf

Danh mục: Báo cáo khoa học

... to synonymous mutations We hypothesized that the lack of non-synonymous mutations was a result of the structural constraints on Gag One would thus expect to see a higher rate of non-synonymous ... nonprogressive HIV- 1 infection J Immunol 2007, 17 9(5): 313 3- 314 3 O’Connell KA, Brennan TP, Bailey JR, Ray SC, Siliciano RF, Blankson JN: Control of HIV- 1 in elite suppressors despite ongoing replication and ... Broadway, Baltimore MD 212 05, USA Page of 7 10 11 12 13 14 Authors’ contributions MS carried out the molecular genetic studies, participated in the sequence alignment and drafted the manuscript TPB...

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Báo cáo y học: " Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: Novel insights into the regulation of Rev nuclear impo" doc

Danh mục: Báo cáo khoa học

... Biol 19 90, 11 1:807- 816 doi :10 .11 86 /17 42-4690-8 -17 Cite this article as: Gu et al.: Intermolecular masking of the HIV- 1 Rev NLS by the cellular protein HIC: Novel insights into the regulation of ... Virol 19 91, 65:70 51- 7055 Malim MH, Bohnlein S, Hauber J, Cullen BR: Functional dissection of the HIV- 1 Rev trans-activator–derivation of a trans-dominant repressor of Rev function Cell 19 89, ... and T2, TCF1, HIV- 1 Tat, HTLV -1 Tax and KSHV LANA [24-29] We previously reported that the ectopic expression of HIC resulted in the mislocalisation of HIV- 1 Tat to the cytoplasm This contrasted...

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Báo cáo y học: "HIV-1 integrase modulates the interaction of the HIV-1 cellular cofactor LEDGF/p75 with chromati" pptx

Danh mục: Báo cáo khoa học

... co-expressing HIV- 1 integrase Because of the universal character of the cofactor role of LEDGF/p75 in HIV- 1 DNA integration (reviewed in [19 - 21] ), we decided to study the effect of HIV- 1 integrase on the ... interaction of HIV- 1 integrase with chromatin bound LEDGF/p75, we evaluated the consequence of increasing concentrations of NaCl on the chromatin binding of HIV- 1 integrase (salt extraction assay) HIV- 1 ... integrase The HIV- 1 co-factor activity of LEDGF/p75 involves its simultaneous interaction with the host chromatin and with HIV- 1 integrase [1, 2] To determine the effect of the ionic strength on the...

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Báo cáo y học: "Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev function" ppsx

Danh mục: Báo cáo khoa học

... University, Israel, 1: 10000); p17 (NIH AIDS Reference Reagents Program, 1: 1000); GFP (Roche, 11 814 4600 01, 1: 1000); flag (Sigma, F1804, 1: 1000); a-tubulin (Sigma, T 516 8, 1: 10000); RecQL -1 (H -11 0) (Santa ... transcription: shaping the landscape of the genome Nat Rev Genet 2005, 6:669-677 18 Marcello A, Dhir S, Dieudonne M: Nuclear positional control of HIV transcription in 4D Nucleus 2 010 , 1: 8 -11 19 Dieudonne ... identification of HIV- 1 RNA associated factors A) Description of the HIV- 1 constructs Above an outline of the fulllength viral genome, below the two constructs used in this work: HIVexo (carrying the...

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Báo cáo y học: " Homonuclear 1H NMR and circular dichroism study of the HIV-1 Tat Eli variant" doc

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... synthesis, purification, Page 10 of 11 (page number not for citation purposes) Retrovirology 2008, 5:83 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 and characterization of ... molar ratios of Tat Eli to Zn2+ from 1: 1 through 1: 16 and the only effect observed was precipitation of Tat at 1: 16 at pH 4.5 and 1: 8 at pH (Figure 3) When there is no precipitation, the CD spectra ... ≥ 5] More than 15 % of the long-range constraints involved the second exon of Tat, and half of those were in relation to the cysteine-rich region showing that the second exon is essential in Tat...

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