imaging invasion and metastasis in vivo
Báo cáo hóa học: " Ezrin promotes invasion and metastasis of pancreatic cancer cells" ppt
... of ezrin in intercalated duct cells, the tubular complexes of CP and PanINs (A) Staining of ezrin in the intercalated duct cells in the paraneoplastic tissues (B-D) Ezrin-positive staining in the ... cells, such as increased protrusions and microvilli Ezrin overexpression inducing Erk1/2 activation The results described above indicate that ezrin is involved in the motility and invasion of MiaPaCa-2 ... experimental metastasis in mouse models were examined to study the role of ezrin in the growth and metastasis of MiaPaCa-2 cells in vivo In the spontaneous metastasis models, the tumor incidences...
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Báo cáo khoa học: "Skin invasion and prognosis in node negative breast cancer: a retrospective study" pps
... ubiquitous use and good cost-benefit balance Sometimes skin invasion cannot be predicted preoperatively Based on our findings, dimpling alone can be a clue for skin invasion Skin invasion is important ... http://www.wjso.com/content/6/1/10 Table 2: Clinical findings in skin in the ipsilateral breast Dimpling Tumor fixing to the skin Nipple retraction Ulcer Edema Post biopsy No abnormal findings 34 30 15 (35.1%) (30.9%) ... [14] Removal of overlying skin is necessary when skin invasion is predicted Conclusion 0.95 Our study suggests that skin invasion has no impact on survival in node negative invasive breast cancer...
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Báo cáo y học: "Human T-cell leukemia virus type 2 post-transcriptional control protein p28 is required for viral infectivity and persistence in vivo" ppt
... needed to understand the function of p28 Such studies will entail identifying the functional domains of the protein involved in localization, protein interactions, and RNA binding as well as precisely ... which introduced a stop codon at amino acid of the p28 ORF and had no affect on the overlapping Tax and Rex amino acid sequence We initially determined whether knocking out p28 altered Tax and/ or ... result in the death of the infected cell in vivo via the induction of apoptosis and/ or host immune response Growing evidence indicates that the HTLV-1 p30 and the HTLV-2 p28 accessory proteins encoded...
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Báo cáo y học: "Oxidized phospholipids reduce ventilator-induced vascular leak and inflammation in vivo" pot
... studies VNB and NL synthesized the OxPAPC and participated in the manuscript discussion JGNG funded in vivo studies KGB initiated, reviewed, and coordinated the study and funded in vivo and in vitro ... experimental group) were agarose-inflated in situ, fixed in 10% formalin, and used for histologic evaluation by hematoxylin and eosin staining as described in Materials and methods Histological analysis ... Involvement of Rho pathway in ventilator-induced lung inflammation and barrier dysfunction In the following experiments, we investigated a role of Rhodependent signaling in lung injury induced by mechanical...
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báo cáo hóa học: " In vitro and in vivo pre-clinical analysis of a F(ab’)2 fragment of panitumumab for molecular imaging and therapy of HER1-positive cancers" pot
... radiolabelings TN performed PET imaging and analysis MWB synthesized the chelate and participated in the design of the studies DEM conceived of and coordinated the studies and assisted with the in vivo ... following i.p injection of the panitumumab F(ab’)2 Imaging studies, g-scinitigraphy and PET, of the i.p tumor model using these two injection routes are pending Studies from this laboratory and ... and 96 h using MONICA (Mobile Nuclear Imaging Camera, NIH, Bethesda, MD, USA) [35] Images were analyzed using NucLear Mac software (Scientific Imaging, Inc., Crested Butte, CO, USA) PET imaging...
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Báo cáo y học: "Long-term cyclical in vivo loading increases cartilage proteoglycan content in a spatially specific manner: an infrared microspectroscopic imaging and polarized light microscopy study" ppt
... using our in vivo joint loading model, we will be able to test different loading patterns to determine thresholds of healthy and damaging loading These thresholds would be useful in designing ... studies will test other durations, peak forces, and frequencies of in vivo joint loading Conclusion We conclude that cyclical in vivo joint loading increases the proteoglycan content of the cartilage ... images of joint cartilage from region of interest (a) Rabbit region of interest MCP joint sections were stained with iron hematoxylin, safranin O, and fast green This combination of stains identifies...
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báo cáo khoa học: " Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging" ppt
... sensoring the in vivo molecular environment surrounding the nanoparticles and tracing the in vivo biodistribution of CLIO in liver, spleen and kidneys [38] Obviously, due to the excellent in vivo imaging ... Biosciences), which absorb and emit in higher wavelength light (ex/em, 774/789 nm), and investigated their applicability in cell labeling and in vivo imaging Results and discussions Preparation ... wavelength for imaging centered at 800 nm, which can be applied to in vitro cell labeling and in vivo imaging Methods Cells, animals and chemicals The RAW264.7 cell line was purchased from the China Center...
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báo cáo khoa học: "Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging" ppsx
... biodegradable and incorporated into hemoglobin in red cells within 30 to 40 days or integrated into other metabolic processes [27] SPIONs tend to aggregate and this has been reduced by coating with ... (DMEM F-12; Invitrogen Inc., Carlsbad, CA, http://www.invitrogen.com ), supplemented with 10% fetal bovine serum (FBS; Invitrogen Inc.), mM l-glutamine (Invitrogen Inc.), 100 U/mL penicillin (Sigma-Aldrich ... display blue intracellular staining (B-F”) Representative images showing dextran immunostaining (green) in MSCs labeled with Feridex and nuclei counterstained with DAPI (blue) (B) Cells incubated...
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Báo cáo y học: " In vivo imaging of the airway wall in asthma: fibered confocal fluorescence microscopy in relation to histology and lung function" potx
... patients and controls These findings indicate that FCFM can be used to capture structural changes in the airway wall in humans in vivo, and might become a Table Elastic fibre pattern grading: Paired ... endobronchial sites The bronchial main carina was chosen as the location for FCFM and biopsy to minimize imaging artefacts resulting from inadequate positioning of the miniprobe superimposed on the ... light-coloured and thick individual lines could be discriminated in FCFM images with score (‘lamellar’) These lines were organized into layers similar to the histological sections The linear and orientated...
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Báo cáo sinh học: "A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma" ppsx
... sacrificed In the past few years, noninvasive imaging techniques such as positron emission tomography (PET), magnetic resonance imaging, and optical imaging methods using fluorescence and bioluminescence ... a protein known to be overexpressed in cancer cells [27], and (4) coelenterazine is much more expensive than D-Luciferin Iyer et al [28] examined noninvasive imaging using PET and BLI in CD-1 ... introduced and increasingly used for temporal and spatial monitoring of transgene expression [11-13] Bioluminescence imaging (BLI) using luciferase (Luc) from the North American firefly Photinus...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... characterized by insulin resistance and insulin secretion deficiency At present, there is no a single medication which treats type diabetes by improving both insulin resistance and insulin secretion ... fusion protein exhibited significant glucose-lowering effect in diabetic mice and may be a promising agent that can treat type daibetes by improving both insulin resistance and insulin secretion ... fusion protein in inclusion body was refolded in urea gradient refolding buffer And then, the refolded protein was incubated with enterokinase to remove the His-tag The fusion protein without...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
... stability and toxicity of PANs, and further investigated the sustained release behavior in vivo of drug loading in PANs PA was firstly synthesized and characterized by FT-IR and 1H NMR, and then ... novel hydrophobized polysaccharide/oncoprotein complex vaccine induces in vitro and in vivo cellular and humoral immune responses against HER2-expressing murine sarcomas Cancer Res 58:3385–90 Gustafson, ... sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study was conducted by the routine method (Mross et al., 1988; Bibby...
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Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx
... prostaglandin D2 in adipogenesis in vivo Y Fujitani et al indicate that overexpression of H-PGDS increases insulin sensitivity in vivo Adipocyte differentiation ex vivo Finally, we examined whether ... glucose, leptin and insulin, and insulin sensitivity, in TG mice After weeks of normal or HF diet, serum levels of triglyceride, glucose, leptin and insulin were deter- 1 Duration (week) 0 mined (Fig ... adipogenesis, and increased insulin sensitivity when on the HF diet Thus, we show, for the first time, that PGD2 is involved in the activation of adipogenesis and regulation of insulin sensitivity in vivo...
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Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids ... [10]) There are different RAR and RXR subtypes (a, b and c), and each subtype exists in multiple isoforms [11] at-RA binds to the ligand-binding domain of RARs, which induces heterodimer formation ... experiment The subsequent incubation with the indicated retinoids was then terminated at the indicated time points by washing with NaCl ⁄ Pi and lysis of the cells using mL of Trizol (Invitrogen) per well...
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Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx
... Desbuquois et al Insulin-induced translocation of Grb14 in rat liver A H N B M LP S PM GE –ins Grb14 – ins Grb14 + ins –ins + ins + ins – ins EEA1 + ins Na+K+ATPase Calnexin + ins number: 10 11 ... [13], and tankyrase [15] The binding of ZIP to Grb14, by recruiting protein kinase Cf, enhances the serine phosphorylation of Grb14 and its inhibitory effect on IR kinase and insulin signaling ... liver insulin signaling, and that it may represent an interesting molecule with which to regulate liver insulin action Experimental procedures Materials Porcine insulin, recombinant human insulin,...
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Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
... missing links in insulin signal transduction? FEBS Lett 410, 3–10 Dedhar, S., Benjamin, W & Gregory, H (1999) Integrin-linked kinase (ILK): a regulator of integrin and growth-factor signalling ... that of PKB protein and activity in the insulin treated and S-PKB cells, indicating that SLex might be regulated by other insulin-induced factor(s) in addition to PKB signalling pathways It is ... migration and invasion (A) Inhibition by mAbs of the adhesion to HUVECs of untreated control and insulin treated cells (B) Inhibition by monoclonal antibodies of the migration and invasion of insulin...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... UP12 is a stress protein DISCUSSION In this work, we have identified two proteins GatY and UP12 as putative in vivo substrates of the chaperonin GroEL In addition to its in vivo interaction with GroEL ... members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids long; ... acrylamide in the separating and stacking gels, respectively In order to estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing...
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Báo cáo khoa học: Efficient and targeted delivery of siRNA in vivo pdf
... required [12] In this minireview, major factors in determining overall RNAi efficiency in vivo are introduced Moreover, up-to-date progress in achieving efficient and targeted siRNA delivery in vivo, particularly ... successfully inhibited the expression of VEGF, thereby suppressing the growth of hepatocarcinoma tumors in mice [87] In addition, no noticeable increase in inflammatory cytokines, including interferon-a and ... obtained by in vitro transcription reaction and, therefore, avoid contamination by cell or bacterial products Promising in vitro and in vivo RNAi was obtained using siRNA that was directly linked...
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Báo cáo khoa học: Selectins and glycosyltransferases in leukocyte rolling in vivo pdf
... carrying the sialyl Lewis X (sLex) determinant During in ammation, the main in ammatory selectin ligand, P-selectin glycoprotein ligand-1 (PSGL-1), interacts with P- and L-selectin under in vivo ... rolling to firm leukocyte arrest [14] During in ammation, E- and P-selectin bind to selectin ligands expressed on rolling leukocytes (Table 1) In vivo studies using mice deficient in P-selectin ... nodes in core GlcNAcT-I– ⁄ – P- and E-selectin ligand-dependent rolling dramatically reduced in FucT-VII– ⁄ – during in ammation in vivo L-selectin-dependent rolling almost completely absent in...
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