... histidine residues are central to copper binding in many proteins they probably form part of the copper binding sites in this protein Although there are four histidines inthe C-terminal, another ... partitioning of the protein into an aggregated state The effect of copper binding on the secondary structure was determined using CD inthe far UV To prepare proteins without Cu2+, part of each protein ... propose that in globular proteins which bind Cu2+ specifically, the metal binding can be protective against amyloid fibril formation Therefore, maintaining correct metal ion protein interactions...
... of the Family I soluble PPases We also provide information concerning the kinetics and properties of the enzyme More strikingly, we show a novel role of the PPase enzyme inthe development and ... postcultures and photographs were taken Enzyme kinetic study The Km (Michaelis constant) and Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins inthe standard ... wrapped in cotton gauze and suspended in NaCl/Pi containing 100 lgÆmL)1 penicillin/ streptomycin at 37 °C for h After incubation the tissues were removed, andthe larvae were collected from the bottom...
... of the various bands, the proteins were extracted from the native gel and analysed by SDS/PAGE (Fig 1B) Indeed both proteins form a complex and run together as they are present inthe same band ... with a binding constant Kd of 2.75 lM, and that the amino-terminal domains of the two peroxins were responsible for this interaction Therole of TbPEX14 in glycosome biogenesis received further ... was located inthe band This may be due to diffusion of some complexed protein as the band migrates inthe native gel Band contains the excess of TbPEX14-N,H In a second experiment, the same two...
... pharmaceutical industry, independent investigators and major contributors to research investment such as the Bill & Melinda Gates Foundation The IAS, under both the ILF and our work with the Bill & Melinda ... also increasingly involved inthe development and delivery of a range of education and training programmes designed to help individuals working in HIV advance professionally, from administering the ... activists and others working on the frontlines of the response The IAS encourages all professionals working inthe HIV/AIDS field to become members We are convinced that inthe struggle against this...
... determined the structural and functional properties of the protein–protein interaction between v-KIND and MAP2 We defined the binding core regions for the v-KIND–MAP2 interaction and showed that the ... branching Results The KIND2 domain of v-KIND has a unique ability to localize to dendrites via MAP2 binding, which is absent inthe KINDs from other KIND-containing proteins To examine the dendrite ... obtained indicate that the KIND2 domain is necessary and sufficient for the targeting of v-KIND to dendrites of neurons To investigate the MAP2-binding characteristics of the KIND domain protein...
... containing multiple ankyrin repeat motif domains and a single KH domain similar to the MASK gene found in Drosophila Drosophila MASK (dMASK) has been implicated in cell survival and may play a role ... melanogaster and 48% similarity with Anopheles gambiae Interestingly, all the compared species contained the12 ankyrin repeat domains and exhibited high homology between them, suggesting a conserved ... VBARP and Vpr is specific The input panel represents the amount of protein used in this assay indicating that an equal amount of protein was used in all our samples and that the lack of interaction...
... apoptotic and are eliminated Several cytokines, including IL- 2, IL- 4, IL- 7, and IL- 15, are effective in preventing the death of activated T cells Of these, IL- 4 and IL- 15 are the most effective [11] Inhibition ... either the common γ chain or the IL- 13 receptor α chain Binding of IL- 4 results inthe tyrosine phosphorylation of signal transduction molecules including motifs similar to those involved in insulin ... required to initiate intracellular signaling (b) The sIL-4R consists of the extracellular portion of IL- 4Rα It retains the ability to bind IL- 4 with high affinity and high specificity and thereby...
... function inthe brain BGT-1 is capable of transporting both betaine and GABA, as examined in Xenopus oocytes and various cell lines But the physiological substrate of BGT-1 inthe brain remains to ... The biosynthesis of taurine is primarily from cysteine derived in part from methionine via the cysteine sulfinate pathway, which includes oxidation of cysteine to cysteine sulfinate by cysteine ... likely to be the physiological substrate of BGT-1 inthe brain, andthe presence of BGT-1 in pyramidal neurons suggests that these neurons utilize betaine to maintain osmolarity Meanwhile, the present...
... acetylcholine, dopamine and norepinephrine During signal transduction, the movement of calcium ions into the axon terminals induces the docking of neurotransmitter filled vesicles onto the pre-synaptic ... 1998) Inthe monkey brain, iPLA2 immunolabeling was generally observed inthe telencephalon including the cerebral cortex, septum, amygdala and striatum while the diencephalon which includes the ... latency timings inthe passive avoidance test, and higher number of errors inthe attention set-shifting task, indicating deficits in working memory and attention, respectively Together the results...
... within days in every run Assuming the zero-order kinetics, the average cadmium removal rates within the days calculated from the linear approximations of the plots in Fig were estimated as 0 .12 ... concentration in giant reed as a result of Run 6, indicating that the cadmium was highly concentrated inthe rhizome The transportation index, Ti, indicates the ability of the plant to translocate the ... cadmium, and is synthesized from glutathione (GSH) Inouhe et al (1994) reported that the cadmium binding complex containing PCs has an important role for the cadmium-tolerance of seedlings of...
... between the writer andthe readers, andthe other between the characters (the main character and his son) In this story, the writer plays therole of the narrator using neutral and daily lexis The ... giving, and (ii) demanding” This means there are two roles in exchange: giving and demanding He goes on to analyze the nature of the commodity being exchange into (a) goods and service and (b) information ... the meaning: “Fred stayed behind, and John ran away.” Therefore, the relationship is symmetrical Andthe sentences “Joe watched TV, and Mary washed the clothes” and “Mary washed the clothes and...
... protein were examined first [20] We observed that the intracellular calcium level increased in OccWT-overexpressing cells, and that 2-APB inhibited the effects of OccWT Therefore, the increase in intracellular ... analysis of the involvement of wild-type occludin (OccWT) and variant occludin in apoptosis and invasion, as determined by assay, we revealed that exon played a major roleinthe induction of ... wild-type occludin were examined in Chang cells Furthermore, in a comparative analysis between OccWT and OccDE9, the exon region played a significant rolein promoting apoptosis and inhibiting invasion...
... seems, that the fronting, the shoulder andthe tailing is due to acetyl-CoA and not due to any other compound (B) The peaks 3, 4, and were isolated by HPLC and used as substrates for AUH The AUH ... according to the wild-type enzyme and tested using the substrate (E)-3-MG-1-CoA In these experiments, the specific activity of MBP-AUHp40mut was 9% (0.068 UÆmg)1 protein) in comparison to the wild-type ... contain traces of free CoA (peak 1) Nothing is known about the fronting andthe peak shoulder of peak 1, however, the compound is described by the supplier as only approximately 95% pure The tailing...
... solfataricus Furthermore, we report the cloning, the expression andthe characterization of the recombinant protein rBcp2 in order to shed light on itsroleinthe detoxification process and on its catalytic ... pernix thioredoxin-like nor other types of thioredoxins were found In this study we examined the involvement of the peroxiredoxin Bcp2 in oxidative stress inthe hyperthermophilic aerobic archaeon ... 2-Cys Prxs, the largest Prx class, is their second redox-active cysteine, the resolving cysteine (Cys-SR) In typical 2-Cys, Sp at the N terminus and SR at the C terminus of the protein belong to...
... (4NPGlc), incubating for 30 at 37 °C, and measuring the release of 4NP Protein assays and protein sequencing Total protein in crude and semipuri®ed samples was estimated using the Pierce Protein Assay ... arbutin or salicin as substrate; 100% C initial, increasing D to 20% (10 min), 50% (15 min), 100% (5 min), held at 100% (5 min); cyanodin glycosides; 5% B/95% A initial (5 min), increasing B ... during the PCR The positive clone was digested by a combination of XhoI and BamHI, and subsequently the cDNA insert was puri®ed using the Qiaquick PCR puri®cation kit and ligated into the XhoI and...
... Engineering in providing services to the government, the public, andthe scientific and engineering communities The Council is administered jointly by both Academies andthe Institute of Medicine ... cosmic rays, moving at close to the speed of light (c) and infiltrating in through the magnetic fields inthe solar wind from the surrounding interstellar space They are primarily protons plus ... wind Thus, exploration of the outer heliosphere offers the opportunity to learn about both the interplanetary andthe interstellar medium, andthe manner in which they interact The detailed interaction...
... and Tien Giang) Since the work is being continued, the full report will be presented next year The available data will also be incorporated into the training manual Objective activities: Training ... post-harvest institutions to highlight the project objectives and also to obtain input from the extension workers on grain losses in relation to harvesting, drying and milling practices in Mekong Delta Introduction ... improve the milling efficiency We have commenced to collect the data of milling losses in two provinces The milling loss data were collected directly from three milling plants in each province (Kien...
... participated inthe training session The harvesting and drying demonstrations and results obtained inthe previous research were presented during these sessions Dr Vinh Truong, Mr Tran van Khanh, and ... Thanh Nghi were the training instructors Figure 7: Full of the participants inthe meeting hall at the training lesson in 25th February 2007 i Study the performance of milling plants and solutions ... the milling efficiency We have collected the data of milling losses in two provinces from three milling plants in each province (Kien Giang and Tien Giang) The same information was reported in...
... Ho Chi Minh city CARD Project 026/VIE05 Report on training program in July-2007 At Kien Giang Province Project: INVESTIGATION OF RICE KERNEL CRACKING ANDITS CONTROL INTHE FIELD AND DURING POST-HARVEST ... after training lesson Figure 11a: Visit the combine harvester at Tan Phat A co-operative, after training lesson Figure 11b: Visit the combine-harvester at Tan Phat A co-operative, after training lesson ... Science and Technology Department, Giồng Riềng district, Kiên Giang province + 189 farmers of Giồng Riềng district Figure 2: Full of the participants inthe Meeting hall at the training lesson...
... Sept-2007 At Can Tho Province (Vinh Thanh & Thot Not Districts) Project: INVESTIGATION OF RICE KERNEL CRACKING ANDITS CONTROL INTHE FIELD AND DURING POST-HARVEST PROCESSES INTHE MEKONG DELTA OF ... 8:00 – 8:10: Declaration the reason of training program – Introduction the delegates, participants 8:10 – 8:15: Introduction the content of the training program (Dr Vinh Truong) 8:15 – 8:20: ... Figure 2: Full of the participants inthe Meeting hall at the training lesson on 22/09/2007 Figure 3: Harvesting time lesson, by Truong Vinh, Nong Lam Univiversity Figure 4: Harvesting method lesson,...