if the scalar dot product of two vectors is negative
Tài liệu Báo cáo khoa học: Comparison of the substrate specificity of two potyvirus proteases doc
Ngày tải lên :
19/02/2014, 16:20
... homology model of TVMV protease
were used to interpret the kinetic data. A comparison of the two structures
and the experimental data suggested that the differences in the specificity
of the two enzymes ... from
results of modeling, the Arg side chain of the substrate
may partially compensate for the loss of the Lys side
Table 1. Comparison of the specificity of TEV and TVMV proteases. The relative ... site-directed
mutagenesis of putative specificity determinants. A
comparison of the natural cleavage sites for the two
enzymes, together with the results of kinetic analyses
reported here, indicate that the residues...
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Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf
Ngày tải lên :
15/03/2014, 09:20
... expected on the basis of
the complete identity of the G-site sequences, the same
K
m
for GSH was registered for both isoforms
(0.5 mm), which is in general agreement with other
published K
m
values ... charge of the r-complex o-nitro group [33].
The comparison of the crystal structure of the rat M1-
1 GST in complex with the transition state analog
and with the product reveals two completely different
binding ... shown
that the catalytic reaction of GmGSTU4-4 is barely
sensitive to the nature of the leaving group, as the sub-
stitution of the chlorine atom with the more electro-
negative fluorine in the CDNB...
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Báo cáo Y học: Characterization of the self-splicing products of two complex Naegleria LSU rDNA group I introns containing homing endonuclease genes pdf
Ngày tải lên :
18/03/2014, 01:20
... support the hypo thesis that the His-Cys motif is
directly involved in the two zinc ion coordination sites [5].
Whereas I-NaeI contains a His-Cys box typical of the two
zinc binding motifs, the I-Nm ... splicing
of the ribosomal RNA precursor of Tetrahymena: involvement of
a guanosine nucleotide in the excision of the intervening sequence.
Cell 27, 487–496.
27. Michel, F. & Westhof, E. (1990) ... predicted t hese RNA species to
represent the 5Â half and the 3Â half of the excised intron, an
assumption based on the estimated s ize and on the fact that
the similarly organized Ppo.L1925 intron...
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![báo cáo khoa học: " Defining the effect and mediators of two knowledge translation strategies designed to alter knowledge, intent and clinical utilization of rehabilitation outcome measures: a study protocol [NCT00298727]" docx](https://media.store123doc.com/images/document/2014_08/12/medium_zgr1407709323.jpg)
Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
Ngày tải lên :
16/03/2014, 11:20
... interesting that the )10 sequence is
located inside the iron box of desA (nucleotides 7–12
of the iron box), explaining the regulation of desA
expression by binding of DmdR1 to the iron box. The
same ... and this regulation is mediated by binding of the
regulatory protein DmdR1 to the iron box located in
the promoter region of the desABCD cluster. Elec-
trophoretic mobility-shift assays of the ... polycistronic
mRNA (see below), allowing complementation of the
desA mutant, even if expression of the endogenous des-
BCD genes is disturbed in the DdesA mutant.
Fig. 2. Lack of desferrioxamine production...
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Báo cáo khoa học: The pivotal regulator GlnB of Escherichia coli is engaged in subtle and context-dependent control potx
Ngày tải lên :
23/03/2014, 04:21
... indicate the SEM. The dotted line is a
result of two linear regression fits of the data points. The extra
abscissa on top of the figure indicates the molar ratio of P
II
*-tri-
mer ⁄ ATase-monomer. The ... activity of the
protein at the bottom of the hierarchy depends on the
modification state of that protein. The advantage of
modulating the activity of a protein by a cascade-type
of regulation rather ... puzzle of a func-
tional explanation for the existence of GlnB: why
should control by P
II
* be absent altogether, and what
then is the function of the cascade and of its pivot
GlnB? The problem is...
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Báo cáo khoa học: Hydrogen bond residue positioning in the 599–611 loop of thimet oligopeptidase is required for substrate selection pdf
Ngày tải lên :
23/03/2014, 06:20
... of the M3 family of metallopeptidases [1–3].
This zinc-binding motif causes the attack of an acti-
vated water molecule at the carbonyl carbon of the
scissile peptide bond and the formation of ... Tyr612 is necessary for the hydrolysis of this substrate.
Relative to other substrates, the bradykinin derivative is also unaffected by
Gly mutations in the loop. This distinction suggests that the ... mutagenesis
studies [17], is more than 8 A
˚
from the active site. The
closed form orients the phenol oxygen of this residue
within hydrogen bonding distance from the carboxyl
group of the scissile peptide...
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Tài liệu Đề tài " On the volume of the intersection of two Wiener sausages " ppt
Ngày tải lên :
14/02/2014, 17:20
... yields the claim in (2.80). This completes the proof of
Lemma 6.
This completes the proof of Proposition 4 and hence of Theorem 1.
3. Proof of Theorem 2
In this section we indicate how the arguments ... and
generalization of the proof of Proposition 3 in [3]. We outline the main steps,
while skipping the details.
Step 1. One of the basic ingredients in the proof in [3] is to approximate the
volume of the Wiener ... which is the factor in the second term on
the right-hand side of the analogue of (2.86). The integral on the right-hand
side of (3.9) is of order
1/2
log(1/). Hence we get C
5/6
log(1/) for the...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
Ngày tải lên :
19/02/2014, 06:20
... fragments F and I. The number at
the right of each RT-PCR product represents the length of the prod-
uct. The scheme also shows the location of the reverse primer
DN+89 used in another starting exon-specific ... generation of protein isoforms.
The 5Â-UTR of eukaryotic mRNA inuences the initi-
ation step of protein synthesis and thereby in part
determines the translational efficiency of the transcript
[26]. ... a 476-bp 5Â-RACE
DNA product, of which the 3Â 380 bp were identical to
exons 13 from the 5Â-end of the reverse primer
DN+231 to the 5Â-end of the region where the
sequence of exon 1 reported previously...
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Tài liệu Huntington’s Borghese Style Urn as a Study of Two-Dimensional Art & The Production of a Piece of Two-Dimensional Art pptx
Ngày tải lên :
19/02/2014, 10:20
... Relief Sculpture in Two Parts
Sculpture Art Lesson
Vocabulary
aesthetic The science of the “beautiful” in a work of art. The aesthetic appeal
of a work of art is defined by the visual. Social, ethical. ... are fish, birds,
flowers and trees). Try to keep the designs simple.
V. Procedure
1. Remove the layer of paper from the top side of the drywall (this is the side
that has the lighter color of paper). ... Once the paper is removed, have the students copy their design on the
surface of the drywall using a pencil. With crayons or pencil, color the parts
of the design that will remain.
3. Next have the...
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