if the scalar dot product of two vectors is negative it means that
Tài liệu Báo cáo khoa học: Comparison of the substrate specificity of two potyvirus proteases doc
Ngày tải lên :
19/02/2014, 16:20
... enzymes with altered specificity by site-directed
mutagenesis of putative specificity determinants. A
comparison of the natural cleavage sites for the two
enzymes, together with the results of kinetic ... homology model of TVMV protease
were used to interpret the kinetic data. A comparison of the two structures
and the experimental data suggested that the differences in the specificity
of the two enzymes ... effects
of the mutations. Hence, the results are consistent with
the predicted role for these residues (based on crystal
structure) in the interaction with substrate. The loss of
activity of the...
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Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf
Ngày tải lên :
15/03/2014, 09:20
... expected on the basis of
the complete identity of the G-site sequences, the same
K
m
for GSH was registered for both isoforms
(0.5 mm), which is in general agreement with other
published K
m
values ... atom with the more electro-
negative fluorine in the CDNB molecule did not affect
the k
cat
values. Altogether, these results are consistent
with the idea that the rate-limiting step of the CDNB–
GSH ... electrophilic assistance interactions to the
developing charge of the r-complex o-nitro group [33].
The comparison of the crystal structure of the rat M1-
1 GST in complex with the transition state...
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- 1
Báo cáo Y học: Characterization of the self-splicing products of two complex Naegleria LSU rDNA group I introns containing homing endonuclease genes pdf
Ngày tải lên :
18/03/2014, 01:20
... support the hypo thesis that the His-Cys motif is
directly involved in the two zinc ion coordination sites [5].
Whereas I-NaeI contains a His-Cys box typical of the two
zinc binding motifs, the I-Nm ... primer
extension analysis. The results from the reactions are
presented in Fig. 5 and indicate one major processing site
located 3Â of position U469 of Nae.L1926 (Fig. 5A) and two
sites 3Â of positions C549 ... the regular
splicing pathway. We predicted t hese RNA species to
represent the 5Â half and the 3Â half of the excised intron, an
assumption based on the estimated s ize and on the fact that
the...
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![báo cáo khoa học: " Defining the effect and mediators of two knowledge translation strategies designed to alter knowledge, intent and clinical utilization of rehabilitation outcome measures: a study protocol [NCT00298727]" docx](https://media.store123doc.com/images/document/2014_08/12/medium_zgr1407709323.jpg)
Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
Ngày tải lên :
16/03/2014, 11:20
... con-
tains the repressor-binding site, is boxed, and the ribosome-binding site is underlined. The ATG is shown in bold letters. The reported tran-
scription start site of the S. pilosus desA promoter is ... and this regulation is mediated by binding of the
regulatory protein DmdR1 to the iron box located in
the promoter region of the desABCD cluster. Elec-
trophoretic mobility-shift assays of the ... but there
Fig. 6. Primer extension analysis of the transcription start (TS) point of the S. coelicolor desA promoter. Comparison of the reaction
sequences of the promoter region (T, G, C, A) with...
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Báo cáo khoa học: The pivotal regulator GlnB of Escherichia coli is engaged in subtle and context-dependent control potx
Ngày tải lên :
23/03/2014, 04:21
... chemical
group down the chain. The functional activity of the
protein at the bottom of the hierarchy depends on the
modification state of that protein. The advantage of
modulating the activity of a protein ... P
II
*. The difference in the control of deadenylylation by P
II
*
under the two different growth conditions indicates that control of signal
transduction is adjusted to the growth conditions of the ... indicate the SEM. The dotted line is a
result of two linear regression fits of the data points. The extra
abscissa on top of the figure indicates the molar ratio of P
II
*-tri-
mer ⁄ ATase-monomer. The...
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Báo cáo khoa học: Hydrogen bond residue positioning in the 599–611 loop of thimet oligopeptidase is required for substrate selection pdf
Ngày tải lên :
23/03/2014, 06:20
... k
cat
for these three substrates (see Table 1). Molecu-
lar modeling of the closed form of TOP showed that
the hydroxyl of Tyr612 is within hydrogen bonding
distance of the carbonyl carbon of the cleaved ... demon-
strated that this residue may be important in governing
the differences in substrate selection by these two
enzymes. However, it seems unlikely that the residue
in this position of the loop is responsible ... careful
analysis of the role of the conserved Gly residues in
the 599–611 loop. The flexibility of the loop provides a
means to bring Tyr612 and Tyr605 into close proxim-
ity to the bound substrate,...
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Tài liệu Đề tài " On the volume of the intersection of two Wiener sausages " ppt
Ngày tải lên :
14/02/2014, 17:20
... 1.(2.93)
Substitution into (2.82) yields the claim in (2.80). This completes the proof of
Lemma 6.
This completes the proof of Proposition 4 and hence of Theorem 1.
3. Proof of Theorem 2
In this section ... b
.
(2.5)
Proof. See Proposition 3 in [3]. The function ψ parametrises the optimal
strategy behind the large deviation: (∇ψ/ψ)(x) is the drift of the Brownian
motion at site x, cψ
2
(x) is the density ... < ∞)=
0if1 ≤ d ≤ 4,
1ifd ≥ 5.
(1.2)
What is the tail of the distribution of |R
1
∩R
2
| in the high-dimensional case?
In [9] it is shown that for every d ≥ 5 and δ>0 there exists a t
0
=...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
Ngày tải lên :
19/02/2014, 06:20
... numbers over the diagrams indicate the position of the corresponding nucleotides relative to the translation start site, and the numbers
in parentheses below the diagram show the position of the corresponding ... of the
sequence from position )78 to )54 resulted in the loss
of 50% of the luciferase activity. These results imply
that the )78 to )55 region is required for DNASE1
proximal promoter activity ... University, Japan
2 Division of Medical Genetics and Biochemistry, University of Fukui, Japan
3 Division of Legal Medicine, University of Fukui, Japan
4 Third Division of Internal Medicine, University...
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Tài liệu Huntington’s Borghese Style Urn as a Study of Two-Dimensional Art & The Production of a Piece of Two-Dimensional Art pptx
Ngày tải lên :
19/02/2014, 10:20
... Relief Sculpture in Two Parts
Sculpture Art Lesson
Vocabulary
aesthetic The science of the “beautiful” in a work of art. The aesthetic appeal
of a work of art is defined by the visual. Social, ethical. ... Once the paper is removed, have the students copy their design on the
surface of the drywall using a pencil. With crayons or pencil, color the parts
of the design that will remain.
3. Next have the ... designs that work well are fish, birds,
flowers and trees). Try to keep the designs simple.
V. Procedure
1. Remove the layer of paper from the top side of the drywall (this is the side
that has the...
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