... polyanions(phosvitin)] accelerate both thrombin and activatedprotein C inhibition by PCI; this is consistent with a relatively nonspecific heparin-binding site in proteinC inhibitor. In contrast to AT, there is ... thrombin anion-bind-ing exosite-1 [54,55]. Glycosaminoglycan binding toHC-II is thought to allosterically activate the serpin by displacing the acidic amino terminus from an intra-molecular interaction ... serpin. Heparin cofactor II possesses a unique amino-terminal extension that contains twotandem repeats rich in acidic amino acids with two sul-fated tyrosines (contained in the region encompassedby...
... of hard and satis-fying technical challenges, like data-mining. I got the inspiration for this story while driving from Martha's Vineyardto New York with Patrick and his wife Teresa (Teresa ... mostly in industrial sugars like high-fructose corn syrup. Justknowing how I ate made a gigantic difference. I felt like I ate sensibly, al-ways ordering a salad with lunch and dinner, but I missed ... handed him a tracker-key that his pan was monitoring with tangible suspicion. It radi-ated his identity every few yards, and in the elevator. It even seemed totrack which part of the minuscule...
... 5Â-CATCCAAAATACGCCATGAATATCForward 5ÂrpsO 5Â-TAATACGACTCACTATAGGGGCCGCTTAACGTCGCGReverse 5ÂrpsO 5Â-GCTTCAGTACTTAGAGACForward 3ÂrpsO 5Â-TAATACGACTCACTATAGGGAGACGTAGCACGTTACACCReverse 3ÂrpsO 5Â-GAAAAAAGGGGCCACTCAGGReverse ... and 5Â-AGGATCGCTGGATCCCCGTGTAAAAAAAC, respectively, with pTX367 plasmid [8], creating an NdeIsite that included the translation initiation codon and a BamHI site downstream of the terminator. ... phages (T3 phage indicated by *), respectively.Primer name SequenceForward ompA* 5Â-TAATTAACCCTCACTAAAGGGGTGCTCGGCATAAGReverse ompA105 5Â-GCCATGAATATCTCCAACGAGReverse ompA117 5Â-CATCCAAAATACGCCATGAATATCForward...
... Carpentier for assistance in using beamline BM3 0a. References1 Katayama Y, Hiraishi A & Kuraishi H (1995) Para-coccus thiocyanatus a new species of thiocyanate utiliz-ing facultative chemolithotroph, ... inligand-exchange at alkaline conditions. Despite this, noconformational ligand switching is observed at alkalinepH for the M100K variant. This contrasts with theunfolding data accumulated in this study ... reductionpotential further than may be expected for such an inter-action. Finally, from peroxidase activity assays in theabsence of denaturant the dynamic equilibrium of Metligand dissociation observed in...
... representative met up witha man calling himself “Dirk” at Atlanta’s Hartsfield-Jackson International Airport. “Dirk” was bearing an envelope containing Coca-Cola documents labeled “Classified: ... are willing to pay sums large and small for things—like dirt and water—that they believe have religious or spiritual significance, then clearly spirituality and branding are inextricably linked. ... out a product called Spiritual Water, which is basically purified municipal water, adorned with nearly a dozen different Christian labels. The Virgin Mary bottle, for example, has the Hail Mary...
... this river requires a man who can build dykes against the overflow, who is a naturalist, a geologist, a humanitarian, a diver and a strong swimmer. I love my Bowery. It was my cradle and is ... band of 'toughs,' bartender, and a 'sport' in various mean- ings of the word. The experience certainly warrants the supposition that I have at least a passing acquaintance ... skallybootin' into the infinitesimal ragbag. You can't pull my leg with an old sophism with whiskers on it. You quote Marx and Hyndman and Kautsky - what are they? shines! Tolstoi? his garret...
... rise in their just majesty and take a righteous vengeance for crimes that the law is slow in punishing. I am an advocate of law and order, but I will say to you that less than six months ago ... "'Well, Jerry, if you don't mind,' says the policeman, &apos ;I& apos;d like to disperse the infuriated mob singlehanded. I haven't defeated a lynching mob since last Tuesday; ... motorman, 'anything to oblige. I& apos;ll turn pale and tremble.' "And he does so; and Policeman Fogarty draws his club and says, 'G'wan wid yez!' and in eight...
... quite exhausted by excitement andcontradictory stories. A new pupil witha carriage and a ponyand a maid, and a voyage from India to discuss, was not anordinary acquaintance." ;My name's ... I paid the bills for that ridiculousdoll and her ridiculous fantastic wardrobe. The child was tohave anything she wanted. She has a carriage and a pony and a maid, and I& apos;ve paid for all ... low,soft chair; Emily sat in a chair of her own, with the air of a For more material and information, please visit Tai Lieu Du Hoc at www.tailieuduhoc.org A Little Princess by Frances Hodgson...
... information about the patient, thereby opti-mizing the physicians’ individual information to thepatient. This kind of help was regarded as additional help. The nurse appointed as the NN also had ... witha range of characteristicsincluding age (median age 63 (range 36–79)), maritalstatus, place of residence and gynecological diagnosis(Table 1). All characteristics in Table 1 emerged in ... presentedsame diversity in characteristics as shown in Table 1.DataNarratives obtained through semi-structured interviewswere chosen as the main data source as this is a good wayof gaining insight...
... of about1 ms, which was evaluated without application of spectraldeconvolution analysis. Using the oxygenation of myoglo-bin as a different indicator, significant flash-inducedabsorbance changes ... Biochem. 269) 2631 can be seen also in the amide I region, indicating conform-ational alterations elicited by the redox transition. In thecarbonyl region one can clearly distinguish positive ... sites of oxidized bovineheart cytochrome c oxidase at 2.8 A ˚. Science 269, 1069–1074.9. Tsukihara, T., Aoyama, H., Yamashita, E., Tomizaki, T., Yamaguchi, H., Shinzawa-Itoh, K., Nakashima,...
... stabilizes Ald binding by hydrogen-bonding ⁄hydrophobic interactions at the C-terminal region of Ald.AbbreviationsAhx, 6-aminohexanoate; Ald, 6-aminohexanoate linear dimer; DD,D-alanyl-D-alanine.FEBS ... Molecular design of a nylon-6 byproduct-degradingenzyme froma carboxylesterase witha b-lactamase foldYasuyuki Kawashima1,*, Taku Ohki1,*, Naoki Shibata2,3,*, Yoshiki Higuchi2,3, Yoshiaki ... HS, Prijambada ID,Negoro S, Urabe I & Okada H (1991) Amino acidalterations essential for increasing the catalytic activityof the nylon-oligomer degradation enzyme of Flavobac-terium sp....
... CGCTGGTTCTTGCCAGTCTCAGTGCCGTGGTTGCTAGGGAT1r TTTAGCACCACGAGCCTGGTCACCGCAACGCTGAGC2r CGCAGAAGCCGTACTTACCACAGCACAGGCAGTTCG3r GACTGGCAAGAACCAGCGCCACAGTAAGCGTCACCAReverse GCTAGGATCCCTAGCAACCACGGCACTable 2. Antifungal activity ... recombinant WAMP- 1a activity against diverse plant pathogens, such aschitin-containing and chitin-free fungi, and Gram-positive and Gram-negative bacteria. The amino acidsequence of a second ... formembrane-active amphiphilic AMPs. The positivecharge ensures accumulation at polyanionic microbialcell surfaces that contain acidic polymers, such aslipopolysaccharide and cell-wall-associated...
... biological activit-ies, including: antiviral, antibacterial, antitumor, antialler-gic, antiherbivore and enzyme inhibitory activity. Someactive lichen substances are used in the pharmaceuticalindustry ... DNA damage isrepaired at a relative high rate in human cells by variousrepair mechanisms including photoreactivation, nucleotideexcision and global repair [19,20]. The ability of the isolatedcompound ... negative cells in five fieldsof a standard hemocytometer.Pyrimidine dimersThe DNA was extracted immediately after irradiation usingWizard Genomic DNA Purification Kit (Promega, Madi-son, WI,...