hãy dùng mũi tên như trong sơ đồ mạch điện hình 21 1a để biểu diễn chiều dòng điện trong các sơ đồ mạch điện hình 21 1 b c d

INVESTIGATION OF THE FUNCTIONS OF p23 AND COAT PROTEIN OF HIBISCUS CHLOROTIC RINGSPOT VIRU

Ngày tải lên : 10/09/2015, 09:03

... 0 .1 × + SDS SSC 65 C if probe >10 0 bp 0 .1% SSC 68 C 0 .1% Techniques for detection of hybridization probes on a blot (Chemiluminecent detection) ... denatured probe was immediately added to pre-warmed 50 C DIG Easy Hyb Hybridizing the DIG-labeled DNA probe to DNA on the blot (10 -16 h +), at 41 C 22 The correct temperature for most hybridization ... of DNA 2.4 .1 Plant genomic DNA extraction Table 2 .1 CTAB extraction buffer Component 10 % PEG 8000 1M Tris-Cl PH7.0 5M NaCl 10 % CTAB 500mM EDTA 10 % sodium lauryl sarcosine ddH O Volume Con 10 ...

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báo cáo khoa học: " Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR" pps

Ngày tải lên : 12/08/2014, 03:20

... expressed in inflorescence but not detectable in mature pollen (miR159a; miR167a ,b; miR16 7c; miR16 9d, e,f,g; miR17 1b, c) , of the miRNAs (miR15 9b; miR15 9c; miR 319 a ,b, c) were barely detectable in both ... RDR3 RDR4 RDR5 AGO7 DCL1 AGO9 AGO6 ACT1 AGO8 AGO5 Marker kb http://www.biomedcentral.com /14 71- 2229/9/87 Marker BMC Plant Biology 2009, 9:87 1. 35 1. 08 0.87 0.60 0. 31 DRB4 DRB3 DRB2 kb 1. 35 1. 08 0.87 ... Arabidopsis 12 -day-old seedlings by RT-PCR ACT7(ACTIN7) was used as a positive control to ensure the quality of RNA and cDNA PCR products amplified from cDNA are indicated by asterisks PCR products...

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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx

Ngày tải lên : 12/08/2014, 04:21

... molecule (ICAM -1) [11 ,12 ], decay-accelerating factor (DAF or CD55) [13 ,14 ] and coxsackie- and adenovirus receptor (CAR) [15 ,16 ] Group B coxsackieviruses (CVB) with its six serotypes, CVB1-6, may enter ... adapted to growth in RD cells binds to decay-accelerating factor (CD55) J Virol 19 95, 69 :19 03 -19 16 Shafren DR, Bates RC, Agrez MV, Herd RL, Burns GF, Barry RD: Coxsackieviruses B1 , B3 , and B5 use decay ... Figure Binding studies for DAF and CAR Binding studies for DAF and CAR A) MOI 0.5 TCID50/cell ofCVB2O, EV7W and CVB5 was incubated with 2.5 × 10 5 cells in suspension for h at 4 C and virus attachment...

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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf

Ngày tải lên : 18/06/2014, 18:20

... EEF1G SDHA TBP BACT 18 sRNA EEF1G B2 M BACT SDHA HMBS PGK1 18 sRNA GAPDH TBP B2 M BACT HMBS PGK1 18 sRNA PP1A* PGK1* GAPDH* * EEF1G** SDHA** TBP B2 M BACT HMBS 18 sRNA EEFIG SDHA TBP B2 M* BACT* HMBS 18 sRNA ... TBP B2 M 18 sRNA EEF1G PGK1 SDHA BACT EEFIG PGK1 HMBS* B2 M* SDHA BACT 18 sRNA HMBS PP1A PGK1 EEFIG GAPDH 18 sRNA BACT SDHA BACT GAPDH TBP HMBS B2 M 18 sRNA PP1A TBP B2 M 18 sRNA GAPDH BACT EEFIG B2 M* PPIA ... PP1A GAPDH HMBS PP1A GAPDH TBP SDHA TBP B2 M PP1A EEFIG PGK1 HMBS PGK1 SDHA HMBS GAPDH PGK* PP1A* GAPDH* TBP PP1A EEFIG SDHA PP1A GAPDH PGK1 PP1A GAPDH SDHA 4th 5th 6th 7th 8th 9th 10 th TBP B2 M...

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Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc

Ngày tải lên : 18/06/2014, 22:20

... sumRGC CMV HHV-6 CAMP SARS YF 1. 41 2.82 1. 70 0.83 1. 65 3. 41 1.38 3.84 1. 88 3.69 1. 42 1. 15 1. 40 0.82 1. 32 1. 63 1. 55 1. 94 1. 06 1. 87 1. 45 1. 19 1. 49 0.87 2.02 1. 69 1. 03 1. 57 0.70 1. 30 1. 38 0.95 1. 66 ... 0.95 1. 66 0.89 1. 31 1.37 1. 08 1. 40 0.84 1. 39 4.79 1. 15 2.04 1. 04 1. 31 1.54 0.96 1. 92 0.80 1. 48 20.09 13 .27 18 .95 9.73 17 .34 sumV 8. 41 14.20 6 .11 8.05 7.03 6.29 6 .19 6.08 10 .33 6.70 Abbreviations: ... published immediately upon acceptance cited in PubMed and archived on PubMed Central yours — you keep the copyright BioMedcentral Submit your manuscript here: http://www.biomedcentral.com/info/publishing_adv.asp...

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Báo cáo sinh học: " Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" potx

Ngày tải lên : 19/06/2014, 08:20

... for Biotechnology Information (NCBI) http:// www.ncbi.nlm.nih.gov/ was conducted Eight HSV -1 [EMBL:X0 318 1 .1] , [EMBL:X04495 .1] , [EMBL:X047 71. 1], [EMBL:X1 411 2 .1] , [DDBJ:AB072389 .1] , [DDBJ:AB070847.2], ... primer sequences [HSV-M13 forward 5'-TGTAAAACGACGGCCAGTAGCCTGTAC- http://www.virologyj.com/content/2 /1/ 61 CCCAGCAT-3'; HSV-M13 reverse 5'-CAGGAAACAGCTATGACCTGGGCCTTCACGAAGA-3'] Cycling temperatures ... stained slides were observed for the presence or absence of Lactobacilli, BV associated organisms, Mobiluncus and clue cells Diagnosis of BV was based on the Nugent Score Cervical swabs were used...

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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx

Ngày tải lên : 19/06/2014, 08:20

... fluid J Virol Methods 2004, 12 1: 217 - 2 21 Whiley DM, Mackay IM, Sloots TP: Detection and differentiation of human polyomaviruses JC and BK by LightCycler PCR J Clin Microbiol 20 01, 39:4357-43 61 Beck ... Fullerton, CA) The supernatant was decanted and the pellet was suspended in Dulbecco's Modified Eagle's Medium (DMEM) and stored at -80 C JCV titers were determined by the HA assay as described elsewhere ... Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acceptance cited in PubMed and archived...

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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot

Ngày tải lên : 20/06/2014, 01:20

... EEF1G SDHA TBP BACT 18 sRNA EEF1G B2 M BACT SDHA HMBS PGK1 18 sRNA GAPDH TBP B2 M BACT HMBS PGK1 18 sRNA PP1A* PGK1* GAPDH* * EEF1G** SDHA** TBP B2 M BACT HMBS 18 sRNA EEFIG SDHA TBP B2 M* BACT* HMBS 18 sRNA ... TBP B2 M 18 sRNA EEF1G PGK1 SDHA BACT EEFIG PGK1 HMBS* B2 M* SDHA BACT 18 sRNA HMBS PP1A PGK1 EEFIG GAPDH 18 sRNA BACT SDHA BACT GAPDH TBP HMBS B2 M 18 sRNA PP1A TBP B2 M 18 sRNA GAPDH BACT EEFIG B2 M* PPIA ... PP1A GAPDH HMBS PP1A GAPDH TBP SDHA TBP B2 M PP1A EEFIG PGK1 HMBS PGK1 SDHA HMBS GAPDH PGK* PP1A* GAPDH* TBP PP1A EEFIG SDHA PP1A GAPDH PGK1 PP1A GAPDH SDHA 4th 5th 6th 7th 8th 9th 10 th TBP B2 M...

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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc

Ngày tải lên : 20/06/2014, 04:20

... sumRGC CMV HHV-6 CAMP SARS YF 1. 41 2.82 1. 70 0.83 1. 65 3. 41 1.38 3.84 1. 88 3.69 1. 42 1. 15 1. 40 0.82 1. 32 1. 63 1. 55 1. 94 1. 06 1. 87 1. 45 1. 19 1. 49 0.87 2.02 1. 69 1. 03 1. 57 0.70 1. 30 1. 38 0.95 1. 66 ... 0.95 1. 66 0.89 1. 31 1.37 1. 08 1. 40 0.84 1. 39 4.79 1. 15 2.04 1. 04 1. 31 1.54 0.96 1. 92 0.80 1. 48 20.09 13 .27 18 .95 9.73 17 .34 sumV 8. 41 14.20 6 .11 8.05 7.03 6.29 6 .19 6.08 10 .33 6.70 Abbreviations: ... published immediately upon acceptance cited in PubMed and archived on PubMed Central yours — you keep the copyright BioMedcentral Submit your manuscript here: http://www.biomedcentral.com/info/publishing_adv.asp...

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báo cáo hóa học:" Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" pdf

Ngày tải lên : 20/06/2014, 04:20

... for Biotechnology Information (NCBI) http:// www.ncbi.nlm.nih.gov/ was conducted Eight HSV -1 [EMBL:X0 318 1 .1] , [EMBL:X04495 .1] , [EMBL:X047 71. 1], [EMBL:X1 411 2 .1] , [DDBJ:AB072389 .1] , [DDBJ:AB070847.2], ... primer sequences [HSV-M13 forward 5'-TGTAAAACGACGGCCAGTAGCCTGTAC- http://www.virologyj.com/content/2 /1/ 61 CCCAGCAT-3'; HSV-M13 reverse 5'-CAGGAAACAGCTATGACCTGGGCCTTCACGAAGA-3'] Cycling temperatures ... stained slides were observed for the presence or absence of Lactobacilli, BV associated organisms, Mobiluncus and clue cells Diagnosis of BV was based on the Nugent Score Cervical swabs were used...

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báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx

Ngày tải lên : 20/06/2014, 04:20

... fluid J Virol Methods 2004, 12 1: 217 - 2 21 Whiley DM, Mackay IM, Sloots TP: Detection and differentiation of human polyomaviruses JC and BK by LightCycler PCR J Clin Microbiol 20 01, 39:4357-43 61 Beck ... Fullerton, CA) The supernatant was decanted and the pellet was suspended in Dulbecco's Modified Eagle's Medium (DMEM) and stored at -80 C JCV titers were determined by the HA assay as described elsewhere ... Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acceptance cited in PubMed and archived...

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Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps

Ngày tải lên : 07/08/2014, 23:22

... isolate 1 50 11 7 11 1 5 1 50 11 7 11 1 5 1 50 0 0 0 0 0 0 10 0 0 0 0 0 ATCC 43890 ATCC 12 795 ATCC 33780 c NCTC 90 01 ATCC 9 610 ATCC 25923 ATCC 2 9 213 ATCC 19 117 ATCC 33090 ATCC 19 119 ATCC 13 124 ATCC ... tgcagaaaattgatgctgct ttgcccaggttggtaatagc JOE-acctgggtgcggtacagaaccgt-BHQ1a ggtaaaggggcttcggtatc tattggctccctgaatacgc Cy5-tggtggtgtagccactgtcccgt-BHQ1a GenBank Accession number (Nucleotide position) X80676 ( 415 – 511 ) ... ivanovii Clostridium perfringens Rhodococcus equi Campylocbater jejuni Campylobacter coli Campylocbater jejuni Campylobacter coli Source Number of isolates Number of detected Sal‡ Number of detected...

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Báo cáo khoa học: "Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome" pps

Ngày tải lên : 09/08/2014, 07:21

... α-catenin ACATENINF1 ACATENINZR caacccttgtaaacaccaat actgaacctgaccgtacaccttctccaagaaattctca Primers for human β-catenin BCATENINF8 agggattttctcagtccttc BCATENINZF actgaacctgaccgtacacatgccctcatctaatgtct ... actgaacctgaccgtacacatgccctcatctaatgtct Primers for human E-Cadherin ECADF8 cagaaagttttccaccaaag ECADZR actgaacctgaccgtacaaaatgtgagcaattctgctt Primers for human γ-catenin gCatF1 aacaagaacaaccccaagtt gCatZr actgaacctgaccgtacatagttacgcatgatctgcac ... E-cadherin in lobular and ductal neoplasms of the breast and its biologic and diagnostic implications Am J Clin Pathol 20 01, 11 5:85-98 Charpin C, Garcia S, Bouvier C, Devictor B, Andrac L, Choux...

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Báo cáo y học: " “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies.”" pptx

Ngày tải lên : 12/08/2014, 02:20

... 8462847 78 3 817 13 15 386 25 202 01 28 18 51 35 647 25 724 10 14 16 73 11 32 29768 12 38 75479 13 35 500 14 48 460 15 78 2 71 16 20 775 17 86 8462847 18 78 3 817 19 230 30902 20 692 21 49 674 22 35 ... time PCR in diagnosing HCMV infection and disease were well described by Carini et al 2007 [11 ] A median of 11 vs 30 cells pp65 positive cells were reported among asymptomatic and symptomatic patients ... unrelated donors and kidney recipients were included under group C in the study (Table 1) In group A, sub-group I consisted of three donors positive for HCMV only by real time PCR whose copy numbers...

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Báo cáo y học: " Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene" doc

Ngày tải lên : 12/08/2014, 02:20

... Research Institute of CAAS, Harbin, China 15 00 01 Received: June 2 010 Accepted: December 2 010 Published: December 2 010 References Mengeling WL: Porcine parvovirus In Diseases of Swine Ninth edition ... contributions CPS and CZhu carried out the molecular probe studies, and drafted the manuscript CZha participated in the design of the study and performed the statistical analysis SC conceived of the study, ... http://www.virologyj.com/content/7 /1/ 353 Page of Table Detection of PRRSV in 41 clinical samples by real-time PCR vs conventional PCR Conventional PCR Positive Negative 11 a 0b 2 1c 9d Real-time PCR Positive...

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báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt

Ngày tải lên : 12/08/2014, 03:20

... 5'GTACGCAATGGAATGGAA CC3' 11 2 bp 5'GGTCTTGCTCTCCATCTG CT3' 5'CGGGCTGTCGTCTCATAC TT3' 11 4 bp 5'ACCAGCACAAATCAAAGG A3' 5'GCCAAAGTATGAGACGAC AGC3' 14 9 bp 5'GTCACTAAGCCATCGGTC GT3' 5'ACACGGACACAACCAGTT CA3' 11 2 bp ... BbrizUBI ubiquitin/ribosomal protein 4e-06/ 28/49 (57%) 5'ACCCTCCTCTTGGTCGTT TT3' 5'AGCCCCTCATTTCTTCTT GG 3' 10 5 bp 5'TAAGGTGGGGCTTGTTTT TG3' 5'ACAGCAGCACATACCACA GG3' 16 4 bp 5'TGAATCTAGTCCATCCGC TTG3' ... TTG3' 5'TCATCAGGCAGGGAAGCT A3' 12 4 bp 5'GGGCATTTTGGGTTATGT TG3' 5'TCCCCACTCGTTGTCATA CC3' 14 6 bp 5'CAGCAAGGGAGGAACCAG TA3' 5'TAGCGCAAGACCATCAAC AA3' 13 0 bp 5'ATGAAGGCGATGAAGGAG AA3' 5'GTACGCAATGGAATGGAA...

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báo cáo khoa học: " Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida" pot

Ngày tải lên : 12/08/2014, 03:21

... GGTAGCACTAGAGACACAGCCTT CAGGCAGGTTAAGGCAAAGC/ CTAGCAAGGTACAGAAACGGC AAGCTCCCACCTGTCTGGAAA/ AACAGATTGCCGGAAGCCA CTTACGACGAGTTCAGATGCC/ TAAGTCCTCAACACGCATGC TGGAAACTCAACCTCCATCCA/ TTTCGTCCATTCCTTCACCTG 13 5 1. 83 ... Mitchell EF1a UBQ CYP CYP EF1a ACT EF1a RAN1 RAN1 RPS13 EF1alpha CYP CYP RAN1 EF1a EF1a SAND RAN1 SAND CYP SAND UBQ SAND RAN1 RPS13 ACT RPS13 ACT RAN1 CYP RPS13 ACT UBQ RAN1 RPS13 ACT UBQ GAPDH ... Efficiency ACT Actin 11 SGN-U208507 (At3 g 12 11 /b> 0 .1) 2e -11 0 TGCACTCCCACATGCTATCCT/ TCAGCCGAAGTGGTGAAAGAG 11 4 1. 75 ± 0.07 CYP Cyclophilin SGN-U207595 (At2 g 211 30 .1) 1. 9e-75 AGGCTCATCATTCCACCGTGT/ TCATCTGCGAACTTAGCACCG...

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Báo cáo y học: " Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR" pot

Ngày tải lên : 12/08/2014, 04:20

... Page of 17 A HPV6 L1 HPV 11 L1 HPV16 L1 HPV18 L1 B X 10 e4 HPV6 ,11 ,16 ,18 L1 X 10 e4 HPV6 ,11 ,16 ,18 L1 X 10 e4 HPV11L1 X 10 e4 HPV6 L1 HPV6 L1 (Cy5) HPV 11 L1 (Texas Red) X 10 e4 HPV6 ,11 ,16 ,18 L1 X 10 e4 ... 10 e3 HPV16 L1 X 10 e2 HPV16 L1 X 10 e1 HPV16 L1 X 10 e6 HPV 11 L1 X 10 e5 HPV 11 L1 X 10 e4 HPV 11 L1 X 10 e3 HPV 11 L1 X 10 e2 HPV 11 L1 X 10 e1 HPV 11 L1 HPV16 L1 (FAM) HPV18 L1 (Hex) 2.00E+06 HPV L1 copy # ... HeLa DNA H2O HPV 11 L1 (Texas Red) HPV6 L1 (Cy5) X 10 e6 HPV18 L1 X 10 e5 HPV18 L1 X 10 e4 HPV18 L1 X 10 e3 HPV18 L1 X 10 e2 HPV18 L1 X 10 e1 HPV18 L1 X 10 e6 HPV16 L1 X 10 e5 HPV16 L1 X 10 e4 HPV16 L1 X 10 e3...

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Báo cáo khoa học: " Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection" pps

Ngày tải lên : 12/08/2014, 04:21

... ability to produce a 10 0-fold increase of fluorescence when bound to double-stranded DNA The binding of SYBR Green to nucleic acid is not sequence-specific and the fluorescent signal produced ... based on SYBR Green DNA dye-binding fluorophore that detect CHIKV infection SYBR Green DNA-based RT-PCR systems are good alternative to fluorescent probe-based RT-PCR techniques and are based ... Value Assay Results Undiluted Diluted 10 -1 105 10 4 9 .11 13 .75 Positive Positive Diluted 10 -2 10 3 17 .89 Positive -3 10 2 22.00 Positive Diluted 10 -4 10 1 26.45 Positive Diluted 10 -5 10 0 28.64 Positive...

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Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps

Ngày tải lên : 12/08/2014, 04:21

... recombinant plasmid pcDNA3 .1- gC was used to establish standard curve as standard DNA of FQ-PCR pcDNA3 .1- gC concentration was determined by taking the absorbance at 260 nm by using a Smartspec ... gC gene copy number can be accurately detected by the established FQ-PCR from inoculated ducks, because the animals were certificated as AHV -1- free by qualitative PCR assay before being infected ... vaccine We are currently studying the dynamic distribution of gC in AHV -1- infected and DNA vaccine-inoculated ducks by using this method We believe that this approach could expedite related AHV-1...

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