human embryonic stem cells international policy and regulation megan allyse and stephen minger
Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
Ngày tải lên :
04/10/2015, 16:03
... 1.1 Embryonic stem cells 1.2 Gastrulation– formation of mesoderm and endoderm in the embryo .8 1.3 Regenerative medicine and embryonic stem cells 10 1.3.1 Diabetes– a candidate ... Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cells and cell lines Figure 1.3 Gastrulation and specification ... μ – Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
Ngày tải lên :
18/06/2014, 15:20
... been reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying ... Background Human embryonic stem (hES) cells possess unique features: self-renewal and pluripotency They can be continuously cultured in undifferentiated status and give rise to differentiated cells and ... either hES cells or adult cells (Figure 6, panel B) For miR-106b, miR-92, miR-93, miR-130a and miR-190, the difference in their expression between EB and hES cells and between EB and adult cells were...
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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
Ngày tải lên :
07/08/2014, 18:21
... Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells Trends Biotechnol 2004, ... layers for human embryonic stem cells Biol Reprod 2003, 68, 21502156 Amit M, Shariki C, Margulets V, Itskovitz-Eldor J Feeder layer- and serum-free culture of human embryonic stem cells Biol ... adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells may...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
Ngày tải lên :
11/08/2014, 12:21
... pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and ... AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
Ngày tải lên :
11/09/2015, 09:57
... relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee ... Hours - hr Human- specific vimentin - hVIM Human embryonic stem cells - hESCs Human ESC-derived chondrogenic cell-engineered cartilage - HCCEC Human palate mesenchymal cell line - HPM Human umbilical ... engineering, and the challenges that need to be overcome before clinical application which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
Ngày tải lên :
11/09/2015, 10:14
... events underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have ... and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) while the induced pluripotent stem ... PUBLICATIONS 161! vii SUMMARY Human embryonic stem cells (hESC) are pluripotent stem cells that have the unique ability to differentiate into cells of the three germ line lineages Hence,...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
Ngày tải lên :
16/09/2015, 15:55
... adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have also been isolated from several other organs such as the brain (neuronal stem ... cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of pluripotent stem cell, ... of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their differentiated...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
Ngày tải lên :
16/10/2015, 11:58
... 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem ... HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15 ... family Page X Chapter Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery
Ngày tải lên :
16/10/2015, 15:38
... literature review and comparison for all three types of stem cells, adult stem cells, embryonic stem cells and induced pluripotent stem cells, embryonic stem cells are inevitable and irreplaceable ... possibility of using human embryonic stem cells and derivatives as cellular model in implant testing for two main reasons One, human embryonic stem cells are the very original cells that our human body ... Biocompatibility 1.2 Stem cells ………………………………………………………………….4 1.2.1 Significance in the use of stem cells 1.2.2 Definition of stem cells 1.2.2.1 Adult stem cells 1.2.2.2 Embryonic stem cells 1.2.2.3 Induced...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
Ngày tải lên :
31/10/2012, 16:57
... An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... Aqua, and DAPI Images were captured, processed, and analyzed using ISIS mBAND/mFISH imaging software (MetaSystems GmbH, Altlussheim, Germany) In the mFISH technique, each chromosome (1–22 and X and...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
Ngày tải lên :
18/06/2014, 15:20
... Abbreviations ES cells: Embryonic stem cells; VEGF-R2: vascular endothelial growth factor receptor type 2; ECs: endothelial cells; MCs: mural cells; hMNCs: human peripheral blood mononuclear cells; MCAo: ... mixture of ECs and MCs derived from human ES cells (hESECs+MCs) The cell mixture was composed of almost the same number of ECs and MCs We resorted the VE-cadherin+ cells from these expanded cells to ... undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), and these vascular cells construct blood vessel...
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báo cáo hóa học:" Human embryonic stem cells hemangioblast express HLA-antigens" pot
Ngày tải lên :
18/06/2014, 15:20
... molecules on murine embryonic stem cells Biol Reprod 1997, 57(3):561-8 Bonde S, Zavazava N: Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients Stem Cells 2006, 24(10):2192-201 ... MHC proteins in human embryonic stem cells Proc Natl Acad Sci USA 2002, 99(15):9864-9 Li L, Baroja ML, Majumdar A, Chadwick K, Rouleau A, Gallacher L, et al.: Human embryonic stem cells possess ... Trypsin (0.05%) and EDTA (Invitrogen), and passing through 22 G needle and 40 μm cell strainer Single cells were resuspended in Stemline II medium at a concentration of 2–5 × 106 cells/ ml and further...
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Establishment of autologous culture systems for human embryonic stem cells
Ngày tải lên :
11/09/2015, 10:00
... of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical cord matrix stem cells ... embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff Bongso, 2005, see Table 2) Each type of stem cells holds its ... (Sorgner, 2007) Embryonic stem cells Mesenchymal stem cells, Hematopoietic stem cells Oligopotent Can differentiate into only a few cells (Sorgner, 2007) Lymphoid stem cells, Myeloid stem cells Unipotent...
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Baculovirus mediated genetic modification of human embryonic stem cells
Ngày tải lên :
11/09/2015, 14:38
... 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell research began with the derivation of hES cell line and ... of human embryonic stem cells Although lentiviral vectors are widely used in gene transfer to human embryonic stem cells, there is still a need for vectors and methods to achieve efficient and ... human embryonic stem cells Manuscript in preparation Zeng J, Du J, Zhao Y, Palanisamy N, Wang S Baculoviral vector-mediated transient and stable transgene expression in human embryonic stem cells...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
Ngày tải lên :
09/10/2015, 11:24
... allogenic) and from the bone marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells ... hESCs-dervied osteogenic cells in bone formation 21 CHAPTER MATERIAL & METHODS 22 2.1 MATERIAL & METHODS Culture and maintenance of Human Embryonic Stem Cells Human embryonic stem cells (hESCs) used ... mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo These cells have the capacity to self renew and can be directed...
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Human embryonic stem cells for genotoxicity testing
Ngày tải lên :
22/10/2015, 21:13
... RESULTS……………………………………………………… 27 4.1 Characterization of human embryonic stem cells …………………… 27 4.2 Temperature tolerance of human embryonic stem cells …………… 30 4.21 Survival rate of hESC after exposure ... reflect human physiology Lack of standardization is also a limitation with the use of primary explanted cells Human embryonic stem cells (hESCs) have been validated as a permanently stable and healthy ... OBJECTIVES……………………………………………………… CHAPTER 2 LITERATURE REVIEW…………………………………………….4 2.1 Human Embryonic Stem Cells (hESCs): Derivation, Isolation and Maintenance of hESCs…………………………………………………………………………………… 2.2 ESCs Characterization…………………………………………………………….6...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
Ngày tải lên :
10/09/2015, 09:08
... HUMAN EMBRYONIC STEM CELL-DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) ... deciphering the mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell-derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner ... This strand is referred to as the guide strand The complementary strand, known as the passenger strand or miRNA*, is degraded in most cases and present in lower levels than the guide strand (Schwarz...
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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
Ngày tải lên :
10/08/2014, 05:20
... and embryonic stem cells to study human hematopoiesis Curr Opin Biotechnol 2005, 16:510-515 Anderson JS, Bandi S, Kaufman DS, Akkina R: Derivation of normal macrophages from human embryonic stem ... Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells Circ Res 2002, 91:501-508 He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ: Human embryonic stem cells develop into ... from human embryonic stem cells Nat Biotechnol 2001, 19:1129-1133 Kaufman DS, Hanson ET, Lewis RL, Auerbach R, Thomson JA: Hematopoietic colony-forming cells derived from human embryonic stem cells...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
Ngày tải lên :
13/08/2014, 23:20
... facilitate human ESC research and to promote respect for human embryos obtained from clinical reproductive endocrinology practice http://www.tbiomed.com/content/2/1/25 Human embryonic stem cells: ... JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Fischbach GD, Fischbach RL: Stem cells: science, policy, and ethics J Clin Invest ... Embryonic death and the creation of human embryonic stem cells J Clin Invest 2004, 114:1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of blastomeres from human embryos...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
Ngày tải lên :
07/03/2014, 12:20
... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time points ... structure modifications and factor recruitment at the murine b-globin locus in uninduced embryonic stem cells (day 0), as well as that of primitive and definitive erythroid cells (days and 12, respectively)...
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