human embryonic stem cells derivation and culture emma l stephenson peter r braude and chris mason
Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
Ngày tải lên :
11/09/2015, 09:57
... scalable and functional cell source for cartilage repair Chondrocytes, fibroblasts, stem cells, and genetically modified cells have all been explored for their potential as viable cell sources ... Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee et al., 2006) Adult ... engineering 2.2.1 Expansion of hESCs Human embryonic stem cells are conventionally co-cultured on mouse embryonic fibroblast (MEF) feeder cells for their self-renewal and propagation However, culture...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
Ngày tải lên :
16/09/2015, 15:55
... mesechymal stem cells and neuronal stem cells Adult stem cells have also been isolated from several other organs such as the brain (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem ... precursor cells These dedifferentiated cells then proliferate and later form new specialized cells of the regenerated organ Stem cells or progenitor cells are the common denominator for nearly all types ... well as transplanted neural stem cells (Reiss et al 2002) Neural stem cells/ neural progenitor cells may also show low immunogenicity thereby raising the possibility for use of donor neural stem...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
Ngày tải lên :
18/06/2014, 15:20
... adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes (NHA) (all from ... upregulated in adult cells HMVEC = human microvascular endothelial cells, HUVEC = human umbilical vein endothelial cells, UASMC = umbilical artery smooth muscle cells; NHA = normal astrocyte and ... culture HMVEC = human microvascular endothelial cells, HUVEC = human umbilical vein endothelial cells, UASMC = umbilical artery smooth muscle cells; NHA = normal astrocyte and LFB = lung fibroblasts...
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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
Ngày tải lên :
07/08/2014, 18:21
... Itskovitz-Eldor J Feeder layer- and serum-free culture of human embryonic stem cells Biol Reprod 2004, 70, 837-845 Anderson GB, Choi SJ, BonDurant RH Survival of porcine inner cell masses in culture and ... introduced into cultured pluripotential cells by retroviral vector Nature 1986, 323, 445-448 63 Robertson EJ Teratocarcinomas and embryonic stem cells: a practical approach IRL Press, Oxford, ... [32,34,35,41,84,46,70], and endodermal origin; pancreatic cells or heparocytes [66,6,57] Furthermore, hES cells, unlikely murine ES cells, can differentiate into trophoblast cells or extraembryonic endoderm [77,85],...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
Ngày tải lên :
11/08/2014, 12:21
... Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, 7:263-269 94 Deng J, Shoemaker R, Xie B, Gore A, LeProust ... hESC and iPSC DNA methylation landscapes are remarkably similar overall, hundreds of differentially methylated regions (DMRs) exist Never theless, only a small fraction of DMRs represents failure ... Teitell MA, Pelegrini M, Plath K, Lowry WE: Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini...
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Establishment of autologous culture systems for human embryonic stem cells
Ngày tải lên :
11/09/2015, 10:00
... Table Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical ... broad groups according to their derivation sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and ... stem cells, Myeloid stem cells Unipotent Retain self-renewal capacity but can only produce one cell type (Sorgner, 2007) Muscle stem cells Apart from the plasticity, stem cells can also be classified...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
Ngày tải lên :
11/09/2015, 10:14
... stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term self-renewal and ... used for daily hESC culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... al., 2007) Live cell visualization of Smo tagged to a fluorescent protein revealed that intraflagellar transport proteins are required to move Smo from intracellular pools to the primary cilium...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
Ngày tải lên :
04/10/2015, 16:03
... 2001) Later studies provided evidence that ES cells likely bear closer resemblance to embryonic germ (EG) cells as several germ cell markers like Dppa3 (Stella) were expressed in ES cells (Zwaka and ... LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cells and cell lines Figure 1.3 Gastrulation ... from fertilization to E6.5 are represented in this image Embryonic Stem (ES) cells are derived from the inner cell mass while epiblast stem cells (EpiSCs) are of epiblast origin Human ES cells...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
Ngày tải lên :
16/10/2015, 11:58
... Hierarchical Cluster Analysis hdFs human- ES-cell-derived fibroblast-like cells IGF-2 insulin-like growth factor-2 iPS cells induced pluripotent stem cells ICM inner cell mass Klf4 Kruppel-like ... generate human iPS cells and the transgenes were lost gradually after reprogramming A single viral vector carrying all four reprogramming factors was used to generate mouse and human iPS cells through ... HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery
Ngày tải lên :
16/10/2015, 15:38
... all three types of stem cells, adult stem cells, embryonic stem cells and induced pluripotent stem cells, embryonic stem cells are inevitable and irreplaceable source to be studied Hence, for ... sub-confulence Over a long term culture, H9 cells were capable of self-renewal and maintained clear margin from surrounding MEF cells (Figure 2.1A, E) The expression of essential intracellular transcription ... possibility of using human embryonic stem cells and derivatives as cellular model in implant testing for two main reasons One, human embryonic stem cells are the very original cells that our human...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
Ngày tải lên :
31/10/2012, 16:57
... break-down and power failure, either in small-scale culture for research or large-scale culture within bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References ... centromerespecific PNA probe displayed green fluorescence In all experimental groups analyzed, there were no chromosomal aberrations Results Survival rate of hESC after exposure to low temperature ... xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor...
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embryonic stem cells, methods and protocols - kursad turksen
Ngày tải lên :
08/04/2014, 12:51
... mL/well for or 24 wells, mL/well for 12 wells, mL/well for wells, mL for 60-mm dishes and mL for 100-mm dishes) Let gelatin solution sit at room temperature for 30 in a tissue culture hood Remove ... Mouse Embryonic Stem Cells Pierre Savatier, H l ne Lapillonne, Ludmila Jirmanova, Luigi Vitelli, and Jacques Samarut Introduction The molecular mechanisms underlying self-renewal of pluripotent embryonic ... Mouse Embryos 10 Gabriele Proetzel and Michael V Wiles 17 Analysis of the Cell Cycle in Mouse Embryonic Stem Cells Pierre Savatier, H l ne Lapillonne, Ludmila Jirmanova, Luigi Vitelli, and Jacques...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
Ngày tải lên :
18/06/2014, 15:20
... autologous transplantation of bone-marrow cells: a pilot study and a randomised controlled trial Lancet 2002, 360:427-435 Rehman J, Li J, Orschell CM, March KL: Peripheral blood "endothelial progenitor ... endothelial cells; MCs: mural cells; hMNCs: human peripheral blood mononuclear cells; MCAo: middle cerebral artery occlusion; αSMA: alpha smooth muscle actin; hESECs+MCs: a mixture of ECs and MCs ... cells may have a potential to be a source for therapeutic vascular regeneration after stroke Abbreviations ES cells: Embryonic stem cells; VEGF -R2 : vascular endothelial growth factor receptor...
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báo cáo hóa học:" Human embryonic stem cells hemangioblast express HLA-antigens" pot
Ngày tải lên :
18/06/2014, 15:20
... Figure Characterization of blast cell-derived endothelial cells Characterization of blast cell-derived endothelial cells A) vascular structures in Matrigel formed by endothelial cells after 24 ... endothelial cells and professional antigen-presenting cells (APCs) in cellular transplants The specific rejection of transplanted organs and tissues is primarily mediated by T cells and occurs mostly ... progenitors as well as form vascular structures in vitro HLA expression of hES cells, EB cells and blast-like colonies (BLCs) To analyze expression of MHC-I proteins on the surface of human ES cells and...
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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES doc
Ngày tải lên :
27/06/2014, 19:20
... these cells Part 8: Pluripotent Alternatives Other Cell Sources Chapters 22-25 describe alternative sources of pluripotent stem cells These include very small embryonic/ epiblast like stem cells, ... Elisabetta Ruaro, Jelena Ban and Vincent Torre Chapter Oligodendrocyte Fate Determination in Human Embryonic Stem Cells 119 Siddharth Gupta, Angelo All and Candace Kerr Chapter Stem- Cell Therapy for Retinal ... cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem Cells - Differentiation and Pluripotent Alternatives, the story begins...
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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES pdf
Ngày tải lên :
28/06/2014, 04:20
... these cells Part 8: Pluripotent Alternatives Other Cell Sources Chapters 22-25 describe alternative sources of pluripotent stem cells These include very small embryonic/ epiblast like stem cells, ... Elisabetta Ruaro, Jelena Ban and Vincent Torre Chapter Oligodendrocyte Fate Determination in Human Embryonic Stem Cells 119 Siddharth Gupta, Angelo All and Candace Kerr Chapter Stem- Cell Therapy for Retinal ... cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem Cells - Differentiation and Pluripotent Alternatives, the story begins...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
Ngày tải lên :
09/09/2015, 17:54
... for neuronal progenitors 13 Figure 1.4 The differentiation of pluripotent stem cells into neuroepithelial stem cells and radial glia 15 Figure 1.5 Correlation of expression profiles of lncRNAs ... cells The neuroepithelial properties that are retained by radial glia include expression of “neural stem cell markers” such as NESTIN, MUSASHI1 (MSI1) and SOX2 Neurogenic radial glia cells also ... 1.2.3 Radial glia cells are neuronal progenitors in vivo During development, radial glia cells function as neural progenitors (Malatesta et al., 2000; Noctor et al., 2001), as well as a scaffold...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
Ngày tải lên :
09/09/2015, 17:54
... nucleus (Figure 6.10) Figure 6.10: Pluripotent lncRNAs are preferentially localized in the nucleus Cellular RNA was separated into the nuclear and cytoplasmic fractions, and qPCR was performed ... mammalian development, and stem cell pluripotency, is primarily controlled by transcription factors In particular, pluripotency is controlled by the core transcription factors OCT4, NANOG, SOX2 and ... were all expressed in brain regions (whole brain, fetal brain, substantia nigra and cerebellum) While expression of RMST and lncRNA_N1 were more confined to brain regions, lncRNA_N2 and lncRNA_N3...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
Ngày tải lên :
09/09/2015, 17:54
... neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation ... an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated by approximately 9-fold when the neural progenitors differentiate ... enhanced neuronal differentiation of ReNVM cells (A) RMST was overexpressed by transfecting the in vitro transcribed RNA (RMST OE) As a control, total RNA extracted from ReN-VM cells were transfected...
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