hgt in creation of o antigen diversity

Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx

Ngày tải lên : 23/03/2014, 13:20

... Taylor, R.L & Conrad, H.E (1972) Stoichiometric depolymerization of polyuronides and glycosaminoglycouronans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups ... structural information may not be biologically relevant to in vivo conditions Application of CE-ES-MS methods for direct analysis of cells from experimental models of infection can overcome these ... 3-amino-3-deoxy-D-quinovose and 2-amino2-deoxy-D-galacturonic acid, in the approximate molar ratio of 1.5 : 1.0 : 0.4 Composition and methylation analyses of the inoculum TSB culture used to prepare...

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Tài liệu Manual Creation of database in windows with oracle 9i ppt

Ngày tải lên : 25/01/2014, 05:20

... sqlplus/nolog and in sql prompt type conn sys/ceylonlinux_suranga as sysdba then you should see that you are connected to an idle instance Now start the instance in nomount mode as, startup nomount ... pfile=d:\db18\initdb18.ora why are you starting the database in nomount mode ? The reason is still we are not created control files “An instance would be started in the NOMOUNT stage only during database creation ... “Navigator” menu item From the window select second radio button saying “Add selected databases from your local tnsnames.ora file” From there select db18 Once you select it you should see OEM console...

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Tài liệu Báo cáo khoa học: Models and mechanisms of O-O bond activation by cytochrome P450 A critical assessment of the potential role of multiple active intermediates in oxidative catalysis doc

Ngày tải lên : 19/02/2014, 16:20

... efrom reductase OOC COO H H OOC COO H O HO OOC COO O COO products OH H+ 3 O O Fe + Rox O (H+) O Fe + Rox OOC H Fe + Rox spectrometric and solvent isotope effect studies with 4methoxybenzoate O- demethylase, ... analysis of the stoichiometry of the NO-forming reaction disclosed a three-electron process, with decomposition of the N-hydroxyarginine intermediate consuming only 0.5 equivalents of NADPH per mol of ... pregnenolone under an atmosphere of 1 8O2 permitted analysis of the pattern of isotope incorporation into the reaction products, best rationalized by invoking the participation of a nucleophilic peroxo-iron...

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Tài liệu DESIGN creation of artifacts in society ppt

Ngày tải lên : 19/02/2014, 17:20

... illogical artifacts An example of Sottsass’s work within Memphis, another bookcase, is shown in Exhibit MEMPHIS One manifestation of cultural phenomena is the emergence of schools of design or ... review of these two perspectives, I synthesize them into the beginnings of a theory of aesthetics for design Finally, I turn to the problem of how to design beautiful artifacts Evolutionary Aesthetics ... unlikely to result in finding a great solution Better solutions are likely to be found in territory distant from the starting point In such environments, we know that parallel exploration using divergent...

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Tài liệu Báo cáo khoa học: A unique variant of streptococcal group O-antigen (C-polysaccharide) that lacks phosphocholine ppt

Ngày tải lên : 20/02/2014, 11:20

... Discussion We previously interpreted reactivity of a streptococcal cell wall polysaccharide preparation with both of two monoclonal antibodies that detect phosphocholine and the backbone of pneumococcal ... cells of this strain display a normal morphology when examined in Gram-stained smears The fact that four out of 43 natural isolates of S mitis biovar were found to lack phosphocholine in the C-polysaccharide ... to grow in the absence of choline have been described [9,10] When grown in a chemically deﬁned medium containing ethanolamine but no choline, such strains generate phosphocholine-free teichoic...

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Báo cáo khoa học: Tyrosine nitration in the human leucocyte antigen-Gbinding domain of the Ig-like transcript 2 protein ppt

Ngày tải lên : 07/03/2014, 02:20

... pKa of the tyrosine hydroxyl group and makes the nitrated tyrosine more hydrophobic and prone to move into more hydrophobic regions [13,26] These modiﬁcations could induce changes in protein structure ... and isoelectric point; carbamidomethylation of cysteine; variable modiﬁcation; and oxidation of methionine HLA-G binding assay rhILT2-Fc was treated with increasing concentrations of SIN-1 for h ... results indicate that NO does not affect tyrosine phosphorylation, which is related to our previous observation that ILT2 maintains its suppressive function in the presence of NO (Fig 5A) Modulation...

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Considering the Creation of a Domestic Intelligence Agency in the United States pot

Ngày tải lên : 15/03/2014, 21:20

... approaches for assessing options The overarching policy results of the assessment, including a discussion of the pros and cons of creating a new intelligence organization, are included in a companion ... to change rapidly may conﬂict with other objectives—including societal goals of intelligence oversight Problems in interagency cooperation In contrast to foreign intelligence, which mainly involves ... questions about the type of information the U.S government has gathered on individuals and organizations in the United States and about how that information has been col- Considering the Creation of...

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Báo cáo khoa học: Structural analysis of lipopolysaccharides from Haemophilus inﬂuenzae serotype f Structural diversity observed in three strains ppt

Ngày tải lên : 23/03/2014, 21:20

... fact that several anhydroforms of Kdo are formed during the hydrolysis by elimination of phosphate or pyrophosphoethanolamine from the C-4 position [40] Anomeric resonances of HepI, 4.15 74.2 ND ... digalactoside expressed in the LPS of H in uenzae is proposed to play some role in evasion of the host immune response by behaving as a mimic of epitopes found on host glycolipids PCR and/or Southern ... hexose residue or a heptose No ions indicating glycylilation could be observed in the spectrum of OS6252 However, the spectrum of OS7290 showed ions at m/z 280 and 292 corresponding to GlyÆAnKdo-ol...

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Báo cáo khoa học: The association of heavy and light chain variable domains in antibodies: implications for antigen speciﬁcity pot

Ngày tải lên : 28/03/2014, 22:20

... [26] proposed a model for the association of VH and VL, taking into account the interface geometry and the packing of residues involved in the interaction However, the study was based on only three ... height of the letters is proportional to the frequency of the corresponding amino acid in the position indicated on the x axis The letters are colored according to the scheme used in Lesk [35] Orange: ... difference in the packing geometry observed in the two clusters has an impact on the conformation of the antigenbinding site We selected two pairs of residues on opposite sides of the binding site...

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Báo cáo hóa học: "Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma" potx

Ngày tải lên : 18/06/2014, 16:20

... Association Declaration of Helsinki Pre-processing of blood samples Blood samples were collected in EDTA-containing tubes Sample processing was performed within hours after blood withdrawal Blood was ... existence of the tumor cell-lymphocyte complex was observed [39] These findings indicate that macrophages or lymphocytes could play an important role in the induction of circulating tumor cell apoptosis ... blood levels of CEA mRNA preoperatively was examined in gastric adenocarcinoma patients Methods Patients Written informed consent was obtained from every patient on the use of blood samples for...

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Báo cáo sinh học: " Serum levels of preS antigen (HBpreSAg) in chronic hepatitis B virus infected patients" potx

Ngày tải lên : 18/06/2014, 18:20

... dilution of known amounts of recombinant S proteins was used to plot a standard curve The amount of HBsAg present in serum was determined according to the amount of recombinant S protein The ... recombinant S protein indeed folded in the proper way compared to native HBsAg Second, known amount of recombinant S protein was applied in anti-preS coated plates instead of serum HBsAg to examine ... following our previous procedure[39] Western blot was carried out to test both the specificity of polyclonal anti-preS using recombinant preS protein and the ability of polyclonal anti-preS in...

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Báo cáo sinh học: " Overcoming viral escape with vaccines that generate and display antigen diversity in vivo Albert García-Quintanilla" pot

Ngày tải lên : 18/06/2014, 18:20

... setting, an extraordinary product with exceptional results may force current policies to change in order to outweigh all other considerations In the opposite situation, it would serve as proof of ... fraction of HIV strains, no matter how immunogenic it is In agreement with this, other teams have proposed to include most of the diversity for a given epitope based on peptide combinations synthe- ... diverse populations [6-8], and (III) the inclusion of multiple strain variants of the same antigen [9] Here I propose a new kind of vaccines that generate diversity in vivo Presentation of the hypothesis...

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Báo cáo hóa học: " Overcoming viral escape with vaccines that generate and display antigen diversity in vivo" doc

Ngày tải lên : 20/06/2014, 01:20

... setting, an extraordinary product with exceptional results may force current policies to change in order to outweigh all other considerations In the opposite situation, it would serve as proof of ... fraction of HIV strains, no matter how immunogenic it is In agreement with this, other teams have proposed to include most of the diversity for a given epitope based on peptide combinations synthe- ... diverse populations [6-8], and (III) the inclusion of multiple strain variants of the same antigen [9] Here I propose a new kind of vaccines that generate diversity in vivo Presentation of the hypothesis...

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o cáo hóa học:" Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" potx

Ngày tải lên : 20/06/2014, 04:20

... inactivation of Tax-2 leading to the inhibition of HTLV-2 retrovirus replication Further studies of subcellular localization unveiled the co-localization of Tax-2 and CIITA both in the cytoplasm ... PBS and blocked for h in PBS containing 0.5% Page of gelatin (Biorad) and 0.5% bovine serum albumine (Sigma), before overnight incubation at 4°C with monoclonal V5 antibodies (Invitrogen) diluited ... impairment of Tax-2 function Subcellular distribution of Tax-2 in presence of CIITA In order to obtain a deeper insight into the mechanism of CIITA-mediated inhibition on Tax-2 function, the subcellular...