heavy light chain bicistronic expression cassette in the pkai51 yeast expression vector

Báo cáo khoa học: Functional role of Bb-chain N-terminal fragment in the ﬁbrin polymerization process pdf

Ngày tải lên : 30/03/2014, 03:20

... situated in the E-domain of the fibrin molecule, the complementary site ‘c’ is suggested to be situated in the D-domain We have tried to model the intermolecular binding between the D-domain of one ... retard the stage of fibrin protofibril formation Discussion The N-terminal fragment of the fibrin b -chain contains regions related to the polymerization process and to binding of thrombin and endothelial ... BbArg14 is involved in another fibrin polymerization site localized at the N-terminal fragment of the fibrin Bb -chain This site is operational before fibrinopeptide B is split off The inhibitory...

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a history of light and colour measurement, science in the shadows - johnston

Ngày tải lên : 05/06/2014, 11:28

... needs of the gas lighting industry from the second half of the 19th century, it was appropriated by the nascent electric lighting industry to ‘prove’ the superiority of their technology By the turn ... technology By the turn of the century the illuminating engineering movement was becoming an organized, if eclectic, community promoting research into the measurement of light intensity The early 20th century ... piece of writing may be read varies inversely as the square of the distance and directly as the illumination of the writing; then the amount of light lost at the greatest obscuration of the sun was...

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Báo cáo khoa học: "Application of light microscopical and ultrastructural immunohistochemistry in the study of goblet cell carcinoid in the appendix" pdf

Ngày tải lên : 09/08/2014, 07:21

... that contain both mucin and secretary granules within their cytoplasm Therefore we agree with the hypothesis of the dual entodermal and neuroendocrine origin of goblet cell carcinoid [1] The ultrastructural ... endocrine cells in appendiceal tumour nests were chromogranin A- (Figure 2a,b), somatostatin- (Figure 2c,d), synaptophysin- (Figure 2e,f) and serotonin-positive The endocrine cells, invading the ... wall of the caecum were all chromogranin A, synaptophysin- and serotonin- (Figure 3) positive The endocrine cells in the appendix and caecum tumour samples were bombesin-, endorphin-, gastrin- and...

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Báo cáo y học: "Resolution of cast nephropathy following free light chain removal by haemodialysis in a patient with multiple myeloma: a case report" ppsx

Ngày tải lên : 11/08/2014, 19:21

... FREELITE is produced by The Binding Site, Birmingham, UK All other authors declare that they have no competing interests In contrast to the case described previously in the literature, our patient ... and the interstitial fibrosis remained unchanged from the first biopsy indicating that there was no progression in situ from the time of the initial biopsy This would be consistent with the observed ... dynamic process in patients where there is urine flow Driven by a high serum light chain load, casts continually precipitate However, in the presence of sustained glomerular ultrafiltrate, they pass...

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Báo cáo khoa học: Inactivating pentapeptide insertions in the ﬁssion yeast replication factor C subunit Rfc2 cluster near the ATP-binding site and arginine ﬁnger motif docx

Ngày tải lên : 07/03/2014, 02:20

... an arginine residue located in Rfc4 (RFC-B) The side chain of the arginine is referred to as an arginine finger and the finger protrudes into the ATP-binding site of the neighbouring subunit The ... whereas insertion F45 disrupts a8 These insertions are likely to affect the positioning of the arginine finger in site D, thereby blocking DNA binding by RFC–PCNA Of the remaining three inactivating ... protein Mapping the insertions onto the structure of Rfc2: insertions into the N-terminal AAA+ domain The 3D structure of the Rfc2 protein comprises three separate domains Domains I and II together...

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Báo cáo Y học: Cold induces stress-activated protein kinase-mediated response in the ﬁssion yeast Schizosaccharomyces pombe pot

Ngày tải lên : 08/03/2014, 09:20

... growth in the cold Surprisingly, however, the cold sensitive phenotype in atf1– or pcr1– strains is not shared by mutants disrupted in either sty1+ or wis1+ (Fig 5), indicating that the role of these ... antibody to the Ha-tag in order to normalize the protein level during the course of the experiment As shown in Fig 1, a thermal downshift from 28 to 15 °C provoked a clear and largely maintained activation ... dehydrogenase, is involved in the synthesis of glycerol, whose intracellular accumulation is important in response to high osmolarity conditions The expression of gpd1+ is induced in S pombe via the Wis1p-Sty1p-Atf1p...

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Báo cáo Y học: Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the ﬁssion yeast Schizosaccharomyces pombe pdf

Ngày tải lên : 08/03/2014, 23:20

... allow the construction of vectors with Ha6H tag fusions at the C-terminus The resulting plasmids were digested at the unique SﬁI site within the ntp1+ coding region (at position 1665) and the linear ... C-terminus) The resulting plasmids were digested at the unique NcoI site within tps1+ (at position 1297) and the linear fragments transformed into haploid strain MM1, to target integration at the ... metabolism in S pombe from those in S cerevisiae MATERIALS AND METHODS Strains and culture media The S pombe strains employed in this study are listed in Table They were routinely grown with shaking...

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Báo cáo khoa học: A mitochondrial cytochrome b mutation causing severe respiratory chain enzyme deﬁciency in humans and yeast doc

Ngày tải lên : 16/03/2014, 22:20

... of the MTCYB gene highlighting the evolutionary conservation of this region of the protein, including the positively charged arginine at position 318 (shown in bold) and the two ﬂanking amino ... complex III to maintain an intact, functioning complex I [24] Studies of the yeast Lys319Pro mutation The study of bc1 mutations in yeast has provided useful insight into the underlying molecular ... conﬁrming the physical interdependence of these two complexes in the inner mitochondrial membrane [24,30] The mutation in yeast similarly disrupts the activity of the bc1 enzyme complex, suggesting...

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the identification and characterization of proteins required for endocytosis in the budding yeast saccharomyces cerevisiae

Ngày tải lên : 14/11/2014, 06:20

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Regulation of cytokinesis in the fission yeast schizosaccharomyces pombe

Ngày tải lên : 14/09/2015, 14:18

... ring producing two daughter cells of equal size The actomyosin ring consists of about 50 different proteins including actin, actin regulatory proteins, type II myosin heavy and light chains The ... Actomyosin-ring assembly Genetic screens have identified a number of proteins, which are essential for the formation and maintenance of the actomyosin ring They include the myosin 16 heavy chain Myo2p; ... of the spot prevent actomyosin ring assembly, implying that the spots may be the precursor of the astomyosin ring (Wong et al 2002) Prior to the formation of the actomyosin ring many of the ring...

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Sterol rich membrane domains and membrane associated proteins in the fission yeast schizosaccharomyces pombe

Ngày tải lên : 15/09/2015, 17:09

... the actomyosin ring is pushed by the ongoing deposition of septum material in the plasma membrane invaginations following the constricting ring However, in both cases one would expect a linking ... actomyosin rings during metaphase and anaphase 66 5.2 cdc15 mutant cells maintain stable actomyosin rings in metaphase 69 5.3 Mid1p is required for actomyosin ring maintenance in metaphase in the ... targeting to the middle of the cell (Fig 3, 60 to 90 min) Filipin also stained the plasma membrane invaginations that follow the constricting ring (Fig 3, 105 min) After constriction of the ring to...

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Báo cáo khoa học: The association of heavy and light chain variable domains in antibodies: implications for antigen speciﬁcity pot

Ngày tải lên : 28/03/2014, 22:20

... at the interface in the light and heavy chain variable domains The red line indicates the clustering with the highest silhouette value (0.47) In the bottom panel, red, green and blue indicate the ... observed a bias in the usage of light chain V germline genes, whereas this was not the case for the heavy chain V genes There is no intersection between the light chain germlines used in cluster A ... remaining ﬁve positions (L41–L44 and L36) are instead located at the interface between the two chains, and the difference in the amino acids occupying them is likely to be related to the packing...

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Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx

Ngày tải lên : 09/08/2014, 01:24

... that the message expression of hyaluronidase-2 in the synovium of OA and RA was approximately four times that in the control synovium This finding suggests that in OA and RA, the protein expression ... of the baseline of fluorescent signal intensity The CT value of the βactin message was used as an internal standard When the target messages were detected in both the control samples and the ... RT-PCR, the ratio for the amount of the message expressed in control samples to the amount of the message expressed in OA or RA samples was determined as a relative expression level Relative expression...

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Báo cáo y học: "Analysis of immunoglobulin light chain rearrangements in the salivary gland and blood of a patient with Sjögren’s syndrome" pptx

Ngày tải lên : 09/08/2014, 03:24

... particular VL chain genes by foreign or autoantigens present in the parotid gland appears to shape the productive VL chain repertoire in the inflamed tissue A restriction of the VL chain repertoire ... accumulate in the parotid gland but cannot clarify the origin of these cells Whatever the primary aberration in the induction of the salivary inflammation, one abnormality relates to the generation ... supported the conclusion that a distinct B-cell subpopulation accumulated in the parotid gland The mutational frequency and the percentage of mutated light chain genes were greater in the productive...

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Báo cáo y học: "Myosin heavy chain and physiological adaptation of the rat diaphragm in elastase-induced emphysema" ppsx

Ngày tải lên : 12/08/2014, 18:20

... band on the basis of its size, to normalize the intensity of the staining for the different sizes of the fragments The final percentage content of each MHC gene in the total sample was then calculated ... gas mixing in panacinar and centriacinar induced emphysema in rats Am J Respir Crit Car Med 1998, 157:237-245 Wright C, Fadia H, Qin A and Baldwin K Analysis of myosin heavy chain mRNA expression ... myosin heavy chain isoforms in type skeletal muscle fibres J Muscle Res Cell Motil 1989, 10:197-205 Petrof BJ, Kelly AM, Rubinstein NA and Pack AI Effect of hypothyroidism on myosin heavy chain expression...

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focuses on expressions of criticizing in American and Vietnamese e-newspapers in the light of Directness/Indirectness

Ngày tải lên : 06/04/2013, 08:43

... criticism in the US e-newspapers have a very clear style They give the main points and state the criticism at the top in the headline and the lead paragraph(s) The body of the articles then adds ... findings Structure of the articles Each of the three main part of an article (the headline, the lead and the body) has its own distinctive style and content 16 4.2.1.1 The headline The headline ... together at the beginning of the sentence, but in other case, they are separated or the writer delays the appearance of the verb Thus, one of the reason people sometimes misunderstand the information...

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Seafood Supply Chain Quality Management: The Shrimp Supply Chain Quality Improvement Perspective of Seafood Companies in the Mekong Delta, Vietnam

Ngày tải lên : 23/04/2013, 09:34

... and the South of Vietnam The structure of Vietnam’s SFC organization The supply chain quality management of interviewed SFCs The fish chain in the Netherlands The shrimp chain in the MD The life ... the shrimp chain in the MD, like fish and meat These chains may be useful cases and a good reference for solving chain problems For instance, when comparing the fish chain in the MD, there are ... within the SFC and within the chain In other words, the framework focuses mainly on SFC activities and chain activities for seafood quality and safety The framework is presented in Chapter Within...

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Expression: In the Red

Ngày tải lên : 29/09/2013, 17:10

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Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression proﬁling ppt

Ngày tải lên : 18/02/2014, 13:20

... only the longest amino acid sequences that included the DNA binding, hinge and ligand-binding domains were used for this analysis, except for the Knirps family members that lack the LBD In D ... of the follicular epithelium in the resulting adults [40] DmE75A and DmE75B have been implicated in deﬁning the transition stages and of the egg chambers through either inducing or suppressing ... vitellogenic female mosquitoes They isolated several clones that code for a single protein Interestingly, during the initial process of identifying the NR family members in the A aegypti genome, we...

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Tài liệu Báo cáo khoa học: Suppressed catalytic efﬁciency of plasmin in the presence of long-chain fatty acids Identiﬁcation of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt

Ngày tải lên : 18/02/2014, 17:20

... acids in the plasmin molecule, the amidolytic activity of two truncated plasmin variants was examined (Fig 8) Miniplasmin (des-kringle 1-4 plasmin) contains the kringle and the catalytic domain ... resulted in a decrease, of up to two-fold, in the kp of plasmin Considering the recently described reversible nature of the plasmin inhibition by oleic acid [3] and the changes of the kinetic parameters ... plasmin activity (e.g nm plasmin) was superimposed on the initial phase of the optical changes in Fig 1A and these can be misinterpreted as an activating effect of oleic acid Therefore, in the...

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