handling and use of chemicals laboratory operations
CRC handbook of laboratory Safety - Chapter 4 docx
Ngày tải lên :
11/08/2014, 04:20
... and availability of reference material on the hazards, safe handling, storage, and disposal of the chemicals found in the laboratory Note that OSHA uses the word “found,” not the phrase “in use. ” ... be safely and easily manipulated by one person ©2000 CRC Press LLC Also, a laboratory uses a variety of chemicals and procedures The scale is such that standard laboratory practices and equipment ... requirements of the OSHA Laboratory Standard and suggest general means by which an organization and/ or laboratory can comply with it Appendix A of the standard provides many recommendations of how...
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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx
Ngày tải lên :
23/03/2014, 03:20
... family is of great interest in terms of structure–function relationships and the evolution of serine proteases Reports of new members provide a more complete picture of the family and potentially ... The aim of the present work was to study the diversity of trypsins in the digestive gland of P argus, with focus on: (a) the position of lobster trypsins within the brachyurins family and (b) ... and (b) features of lobster enzymes that suggest different specificities or interactions with substrates and ⁄ or inhibitors Results and Discussion Characterization of cDNAs and trypsin-like deduced...
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Báo cáo khoa học: Polysaccharide binding sites in hyaluronate lyase – crystal structures of native phage–encoded hyaluronate lyase and its complexes with ascorbic acid and lactose docx
Ngày tải lên :
23/03/2014, 04:21
... the residues of HylP2 and the ligands, indicate that the catalytic site appears to be centred in the proximity of Tyr264 (Fig 5) Indeed, the orientations and spacing of Gln261, Tyr264 and Arg279 ... spacing of 11 A between sub˚ between subsites and 3, respecsites and and 14 A tively The first molecule of ascorbic acid is held at the lower-most subsite consisting of residues LysC179 and GluB167 ... involving O1 and O4 atoms of ascorbic acid, whereas GluB167 Oe2 interacts with ascorbic acid via O2H This site is specific for the binding because of the unique positions of LysC179 and GluB167 and the...
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Báo cáo sinh học: " Rotavirus nonstructural protein 1 antagonizes innate immune response by interacting with retinoic acid inducible gene I" pdf
Ngày tải lên :
18/06/2014, 18:20
... protective mechanisms of rotavirus vaccines and the pathogenic mechanisms of rotavirus are not fully understood [2,3] A better understanding of the pathogenic mechanisms of rotavirus infection, ... wavelength of 700–800 nm and analyzed with Odyssey software The molecular sizes of the developed proteins were determined by comparison with prestained protein markers (Fermentas, Maryland, CA) List of ... Ebola virus, E6 protein of human papilloma virus (HPV), and 3C of enterovirus 71 suppress IFN induction by inhibiting IFN signaling pathways [9-14] Rotaviruses, members of the Reoviridae family,...
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báo cáo hóa học:" Arthroscopic debridement of the osteoarthritic knee combined with hyaluronic acid (Orthovisc®) treatment: A case series and review of the literature" docx
Ngày tải lên :
20/06/2014, 01:20
... OA of the knee is extremely common, with some studies showing that OA of the knee occurs in at least 30% of people after the age of 50 years, and in 80% of people older than 75 years [4,5] Of ... deterioration of SF-36 PCS scores at the month time point Discussion Traditional and labeled use of Hyaluronic Acid (HA) Orthovisc® for treatment of pain due to OA of the knee involves a series of ml ... trial of arthroscopic surgery for osteoarthritis of the knee The New England Journal of Medicine 2002, 347:81-88 Page of (page number not for citation purposes) Journal of Orthopaedic Surgery and...
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báo cáo hóa học:" Stabilization of mid-sized silicon nanoparticles by functionalization with acrylic acid" pptx
Ngày tải lên :
21/06/2014, 17:20
... FTIR and DLS measurements, drafted the manuscript, and participated in the design of experiments HK and HN carried out the XPS measurements and interpretation and contributed to the discussion of ... were filtered again, washed with chloroform, and centrifuged out of chloroform dispersion Finally, the particles were dried overnight To alleviate particle handling in the X-ray photoelectron spectroscopy ... and the particles need to be functionalized to prevent them from reoxidation Three hundred thirty milligrams of silicon nanoparticles were dispersed in methanol and etched with 20 ml of hydrofluoric...
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Titration with Oxalic Acid ppsx
Ngày tải lên :
07/07/2014, 00:20
... Calculate the number of moles of oxalic acid used for NaOH, using the molar mass of H2C2O4 • 2H2O b Use your answer to Question of the pre-lab to determine the number of moles of base needed to ... calculated number of moles of oxalic acid c Calculate the molar concentration of based used Does the amount of water in which the oxalic acid is dissolved affect the outcome of investigation? ... faintest pink color persists, stop and record the final volume in the buret 12 Flush all chemicals down the drain with plenty of water Wash all beakers and the flask, and clean the buret as described...
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Báo cáo y học: "Safety and Effectiveness of two treatment regimes with tranexamic acid to minimize inflammatory response in elective cardiopulmonary bypass patients: a randomized double-blind, dosedependent, phase IV clinical trial." pdf
Ngày tải lên :
10/08/2014, 09:22
... tapered off in steps of 0.02 and μg/kg per minute, respectively Concurrent validation We validated the clinical criteria of IR and found significant differences in evolutionary levels of IL-6 ... was used to compare means between groups in clinical signs (bleeding) and laboratory parameters (D-dimer, creatine kinase and troponin I) at baseline, h and 24 h after surgery For bleeding and ... interpretation, and drafting the manuscript MB, LL, RP and MLM, were responsible for data collection and processing blood simples during the study and provided useful suggestions PM and JMR, was...
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Báo cáo khoa học: " Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution" pot
Ngày tải lên :
12/08/2014, 04:21
... pattern of viral attachment (PVA) of human and AIVs in pig tissues, we focused on the expression in epithelial cells and glands of NE, TE and BE and in bronchioles and alveoli of LE An overview of ... positive, ++: between 41 and 70% of the epithelium is positive, +++: between 71 and 100% of the epithelium is positive -Avian influenza viruses- Of the heterologous viruses, the group of AIVs was least ... duck and chicken viruses Overall, the differences between the virus yields of swine and AIVs were statistical significant in NE, TE and BE at 24 and 48 hpi and in LE at 24 hpi only Titers of A/New...
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Báo cáo y học: "Conventional or physicochemical approach in intensive care unit patients with metabolic acidos" ppsx
Ngày tải lên :
12/08/2014, 19:22
... the (over )use of several medications such as salicylates and penicillin can be a cause of a positive SIG The importance of a raised SIG in clinical practice, however, is unknown Cusack and colleagues ... appear, for example, in the circulation during sepsis and liver failure, and may be a variety of organic and inorganic compounds [8] The use of urealinked polygelines, for example, as the priming ... causes of metabolic acidosis: hyperchloremia, hyperlactatemia and the presence of other unmeasured strong anions Methods The study was conducted in a single, mixed medical and surgical ICU of...
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Báo cáo khoa học: "Circulating anions usually associated with the Krebs cycle in patients with metabolic acidosis" potx
Ngày tải lên :
12/08/2014, 22:22
... and their associated co-factors (Sigma Chemicals, Poole, UK) All the enzymatic assays relied upon the interconversion of NAD+ and NADH or NADP+ and NADPH and used the change in absorbance due to ... patients in total and from 12 control subjects Of the study patients, 13 had DKA at the time of blood sampling and had a lactic acidosis (defined as a blood lactate concentration in excess of mmol/l ... malate and d-lactate levels Patients with acidosis of unknown origin showed significant increases in the concentrations of isocitrate, α-ketoglutarate, succinate, malate and d-lactate The level of...
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Báo cáo y học: "Oxygen consumption is depressed in patients with lactic acidosis due to biguanide intoxication" docx
Ngày tải lên :
13/08/2014, 20:21
... severity of illness and use of catecholamines were at their highest values Patient awakening occurred slowly, well after the normalization of O2 consumption and related variables Heart rate and the ... difference between venous and arterial CO2 content is suggestive of diminished CO2 production Previous studies have demonstrated that severity of illness, use of sedatives and catecholamines, heart ... differences in O2 and CO2 content [39] Some of the limitations of this present study deserve a comment First, we did not include any control group, because of the peculiar characteristics of the study...
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Iron(III) and copper(II) complexes bearing 8 quinolinol with amino acids mixed ligands synthesis, characterization and antibacterial investigation
Ngày tải lên :
02/09/2015, 13:46
... acknowledge Nigerian Institute of Medical Research (NIMR) for kind donation of the standard bacterial strains and Prof Shinya Hayami of Kumamoto University, Japan for the use of magnetic measurements ... bioinorganic of bioactive compounds, we hereby report synthesis, characterization and antibacterial activities of synthesized iron(III) and copper(II) complexes of mixed ligands, 8-hydroxyquinoline and ... sterilized petri-dishes and allowed to stand for some minutes, then a cork-borer with a diameter of 12 mm was used to bore uniform holes on the surfaces of the dried agars and into each hole was...
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Enhanced transdermal delivery with fatty acids and electroporation
Ngày tải lên :
12/09/2015, 10:37
... CONDUCTIVITY MEASUREMENTS OF SOLVENT AND 0.5M FATTY ACID CONTAINING SOLVENT BEFORE AND AFTER THE ADDITION OF PHY 56 v List of Figures FIGURE 5.4 EFFECT OF FATTY ACID AND SOLVENT USED ON THE PERCENTAGE ... 500 V PULSES OF 100 MS DURATION AT INTERVAL OF 900 MS CONCENTRATION OF FATTY ACID USED IS 0.5M AND DRUG VEHICLE USED IS ETHANOL 112 FIGURE 5.23 EFFECT OF CONCENTRATION OF OLEIC ACID ... value of 7.9 and is approximately 75% ionized at the pH of blood and brain (Triggle and Filler, 1998) 27 Literature Review The current clinical roles of PHY include treatment of glaucoma and myasthenia...
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AN0559 optimizing serial bus operations with proper write cycle times
Ngày tải lên :
11/01/2016, 11:51
... microcontrollers is one of the most secure products of its kind on the market today, when used in the intended manner and under normal conditions There are dishonest and possibly illegal methods used to breach ... bytes of data from a sensor and send the bytes to a EEPROM for storage Two cases are shown in Figure 2: case uses a 24C01A with no optimization; case uses a 24LC01B with the available page mode and ... are used in systems for two purposes: storing data and reading back data Read operations are at full clock speed, so the only methods for optimization are to run the clock at maximum frequency and...
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The Transformation of Escherichia coli with Deoxyribonucleic Acid isolated from Bacteriophage Adgt
Ngày tải lên :
28/05/2016, 11:25
... chromosome The absence of this form of heterogeneity and the availability of a variety of markers, all linked, should make Adg DNA useful for an analysis of the chemical structure of the phage chromosome ... Because of t he uncertainty caused by this inactivation, a better criterion of the purity of the phage p repa rat ions in the la t t er stages of purification is given by the r a t io of the ... 260 mfL and the molar ratio of deoxyribose to phosphorus of the >"dg DNA and of the phage are not significantly different (Table 2), the yield of DNA is also about 90% On the other hand, less...
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Tài liệu Báo cáo khoa học: Helicobacter pylori acidic stress response factor HP1286 is a YceI homolog with new binding specificity pdf
Ngày tải lên :
16/02/2014, 14:20
... Stereo view of a cartoon representation of the monomer of HP1286 b-Strands, a-helices and turns are in yellow, red and green, respectively Strands are labeled from A to H Strands B and F, owing ... exception of residues 57–59 of one monomer, which are part of a b-turn connecting two strands Some of the strands present some kinks that break the continuity of the hydrogen bond patterns, and so ... considered to be composed of two parts This happens for strands bB and bF, and, in fact, they have been labeled bB1 and bB2, and bF1 and bF2, respectively Two hundred and two hydrogen bonds among...
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Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt
Ngày tải lên :
18/02/2014, 17:20
... parameters of plasmin in the presence of fatty acids The values of kp and Km were determined from the amidolytic assay of plasmin activity, according to Model I, in the presence of oleate (OA) and arachidonate ... Evaluation of kinetic parameters Fig Comparison of three models for the catalytic action of plasmin in the presence of stearate The p-nitroaniline (P) released in the course of hydrolysis of 40 lM ... mean (symbols) and standard deviation (cross-lines) of four measurements Lines represent best global fit of the data set to the equation of Model I described in the Materials and methods The...
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Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx
Ngày tải lên :
20/02/2014, 02:21
... 5, and were in the range of the Km values for 1,3-BPGA and even up to three times lower for compound Selection of T brucei gGAPDH residues to be mutated and measurement of kinetic parameters of ... that improved activity of this compound may be a result of hydrogen bonding between the hydroxyl of Thr167 of the protein and the amino group of compound No other inhibitor offered such an interaction ... positioning of the active residue Cys166 and the nicotinamide ring of the NAD+ cofactor during catalysis The average isotropic temperature factor values for the main chain and all atoms of the 359...
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