hand free system

... binding Ó FEBS 2003 Cell -free synthesis of a disulfide-containing protein (Eur. J. Biochem. 270) 4781 Efficient synthesis of a disulfide-containing protein through a batch cell -free system from wheat germ Takayasu ... developed a highly productive cell -free protein synthesis system from wheat germ, which is expected to become an important tool for postgenomic research. However, this system has not been optimized ... show that the wheat germ cell -free system can produce a functional scFv with a simple change of the reaction ingredients. We also discuss protein folding in this system and suggest that the disulfide...

... ) and can  boot several operating systems (Linux, uClinux, and others) Softgun: http://softgun.sourceforge.net Virtual ARM system with many virtual on­board peripherals.  Boots Linux. SWARM ­ Software ARM ­ arm7 emulator http://www.cl.cam.ac.uk/~mwd24/phd/swarm.html Can run uClinux 13 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 klibc http://www.kernel.org/pub/linux/libs/klibc/ “Kernel C library” Tiny and minimalistic C library designed for use in an initramfs at  boot time (alternative to initrds). Fine for the creation of simple shell scripts. Ships with small executables for use in shells, built with klibc, and a klcc compiler to compile your own applications. Not elaborate enough to support BusyBox applications. Useful details in  http://en.wikipedia.org/wiki/Klibc 19 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Platform specific toolchains (1) ARM Code Sourcery (supports GNU/Linux, EABI and uClinux): http://www.codesourcery.com/gnu_toolchains/arm/ Also available for Windows workstations. MIPS http://www.linux­mips.org/wiki/Toolchains (useful links) 6 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Training contents (3) Annexes Automatic system generation with BitBake / OpenEmbedded 33 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Other emulators ColdFire emulator http://www.slicer.ca/coldfire/ Can boot uClinux 37 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Minicom (1) Definition: serial communication program Available in all GNU / Linux distributions Capabilities (all through a serial link): Serial console to a remote Unix system File transfer Modem control and dial­up Serial port configuration 50 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Denx Software Engineering http://denx.de Created by Wolfgang Denk, the author of the U­Boot bootloader. Create and support the Embedded Linux Development Kit (ELDK), a complete and well documented development environment. This kit is not only Free Software, it can be downloaded freely by anyone. A great community member and contributor! 15 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Free Software tools for embedded systems GNU / Linux workstation Cross­compiling toolchains 9 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 uClibc http://www.uclibc.org/ from CodePoet Consulting License: LGPL Lightweight C library for small embedded systems, with most features  though. The whole Debian Woody was ported to it ... ) and can  boot several operating systems (Linux, uClinux, and others) Softgun: http://softgun.sourceforge.net Virtual ARM system with many virtual on­board peripherals.  Boots Linux. SWARM ­ Software ARM ­ arm7 emulator http://www.cl.cam.ac.uk/~mwd24/phd/swarm.html Can run uClinux 13 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 klibc http://www.kernel.org/pub/linux/libs/klibc/ “Kernel C library” Tiny and minimalistic C library designed for use in an initramfs at  boot time (alternative to initrds). Fine for the creation of simple shell scripts. Ships with small executables for use in shells, built with klibc, and a klcc compiler to compile your own applications. Not elaborate enough to support BusyBox applications. Useful details in  http://en.wikipedia.org/wiki/Klibc 19 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Platform specific toolchains (1) ARM Code Sourcery (supports GNU/Linux, EABI and uClinux): http://www.codesourcery.com/gnu_toolchains/arm/ Also available for Windows workstations. MIPS http://www.linux­mips.org/wiki/Toolchains (useful links) 6 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Training contents (3) Annexes Automatic system generation with BitBake / OpenEmbedded 33 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Other emulators ColdFire emulator http://www.slicer.ca/coldfire/ Can boot uClinux 37 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Minicom (1) Definition: serial communication program Available in all GNU / Linux distributions Capabilities (all through a serial link): Serial console to a remote Unix system File transfer Modem control and dial­up Serial port configuration 50 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Denx Software Engineering http://denx.de Created by Wolfgang Denk, the author of the U­Boot bootloader. Create and support the Embedded Linux Development Kit (ELDK), a complete and well documented development environment. This kit is not only Free Software, it can be downloaded freely by anyone. A great community member and contributor! 15 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Free Software tools for embedded systems GNU / Linux workstation Cross­compiling toolchains 9 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 uClibc http://www.uclibc.org/ from CodePoet Consulting License: LGPL Lightweight C library for small embedded systems, with most features  though. The whole Debian Woody was ported to it ... ) and can  boot several operating systems (Linux, uClinux, and others) Softgun: http://softgun.sourceforge.net Virtual ARM system with many virtual on­board peripherals.  Boots Linux. SWARM ­ Software ARM ­ arm7 emulator http://www.cl.cam.ac.uk/~mwd24/phd/swarm.html Can run uClinux 13 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 klibc http://www.kernel.org/pub/linux/libs/klibc/ “Kernel C library” Tiny and minimalistic C library designed for use in an initramfs at  boot time (alternative to initrds). Fine for the creation of simple shell scripts. Ships with small executables for use in shells, built with klibc, and a klcc compiler to compile your own applications. Not elaborate enough to support BusyBox applications. Useful details in  http://en.wikipedia.org/wiki/Klibc 19 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Platform specific toolchains (1) ARM Code Sourcery (supports GNU/Linux, EABI and uClinux): http://www.codesourcery.com/gnu_toolchains/arm/ Also available for Windows workstations. MIPS http://www.linux­mips.org/wiki/Toolchains (useful links) 6 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Training contents (3) Annexes Automatic system generation with BitBake / OpenEmbedded 33 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Other emulators ColdFire emulator http://www.slicer.ca/coldfire/ Can boot uClinux 37 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Minicom (1) Definition: serial communication program Available in all GNU / Linux distributions Capabilities (all through a serial link): Serial console to a remote Unix system File transfer Modem control and dial­up Serial port configuration 50 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Denx Software Engineering http://denx.de Created by Wolfgang Denk, the author of the U­Boot bootloader. Create and support the Embedded Linux Development Kit (ELDK), a complete and well documented development environment. This kit is not only Free Software, it can be downloaded freely by anyone. A great community member and contributor! 15 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 Free Software tools for embedded systems GNU / Linux workstation Cross­compiling toolchains 9 Free Software tools for embedded systems © Copyright 2004­2008, Free Electrons Creative Commons Attribution­ShareAlike 2.5 license http:/ /free electrons.com Jan 24, 2008 uClibc http://www.uclibc.org/ from CodePoet Consulting License: LGPL Lightweight C library for small embedded systems, with most features  though. The whole Debian Woody was ported to it...

... suggested that cell -free translation systems might be used as the fundamental basis for cell-like systems. We review recent progress in the field of cell -free translation systems and describe ... S30-directed, cell -free translation systems has improved greatly over the last two dec- ades. In 1988, the continuous-flow cell -free system [3] represented the first demonstration that cell -free trans- lation ... regeneration system Enzymes Substrates (PEP/PK system CP/CK system etc.) ATP/GTP Translation factor Initiation factor Elongation factor Termination factor Fig. 1. The cell -free protein synthesis system. ...

... lin- ear context -free rewriting systems. This paper shows how the class of string languages generated by linear context -free rewriting systems relates to a number of other systems that have ... hypergraph grammars and observe that they are a context -free rewriting sys- tem. Linear Context -Free Rewriting Systems Linear context -free rewriting systems arose from the observation that a number ... free rewriting systems and multicomponent tree ad- joining grammars [6] generate the same string lan- guages. The multiple context -free grammars of [7] are equivalent to linear context-free...

... organisms in cell free expression systems in order to optimize the efficiency of our combined transcription ⁄ translation system, which is based upon E. coli S-30 cell -free extracts. In these systems mRNA ... Therefore free mRNA accumulates in these cell -free systems [13] and excessive secondary structure formation takes place. Results Effect of cold shock proteins on the combined cell -free transcription/translation ... the combined in vitro transcription ⁄ translation system, we investigated the influence of different CSPs on the cell- free protein synthesis with E. coli cell free extracts. For obtaining a more general...

... context -free grammars (pm- cfg's) and multiple context -free grammars (mcfg's) were introduced in (Kasami 1988a)(Seki 1991) as natural extensions of cfg's. The subsystem of ... are parallel multiple context -free grammars (pmcfg's) and lexical-functional gram- mars (lfg's). Pmcfg's and their subclass called multiple context -free grammars (mcfg's) ... PARALLEL MULTIPLE CONTEXT -FREE GRAMMARS, FINITE-STATE TRANSLATION SYSTEMS, AND POLYNOMIAL-TIME RECOGNIZABLE SUBCLASSES OF LEXICAL-FUNCTIONAL...

... corpus. Translation: After that coal dust had caught fire. 2 Linear Context -Free Rewriting Systems Linear Context -Free Rewriting Systems (LCFRS; Vijay-Shanker et al., 1987; Weir, 1988) subsume a wide ... IWPT, pages 104– 116. Daniel Gildea. 2010. Optimal parsing strategies for linear context -free rewriting systems. In Proceedings of NAACL HLT 2010., pages 769– 776. Carlos G ´ omez-Rodr ´ ıguez, ... Marco Kuhlmann, and Giorgio Satta. 2010. Efficient parsing of well- nested linear context -free rewriting systems. In Proceedings of NAACL HLT 2010., pages 276– 284. Carlos G ´ omez-Rodr ´ ıguez,...

... cell -free expression system. Keywords: cell -free protein s ynthesis; human cyclophilin A; 15 N-HSQC spectrum; NMR; selective stable isotope labe- ling. High-yield, cell -free protein synthesis systems ... E. coli expression system by construction of an artificial gene. The protein yields obtained in vitro for hCypA with the T7 promoter system were about 1.5–1.8 mgÆmL )1 of cell -free reaction medium, ... measurement. Results Cell -free protein synthesis enhanced by T7 RNA polymerase Prior to the preparation of 15 N-labelled samples of hCypA and analysis by NMR spectroscopy, the performance of the cell -free expression system...

... production right -hand side in LCFRSs with fan-out at most 2. This results in asymptotical run- ning time improvement for known parsing algorithms for this class. 1 Introduction Linear Context -Free Rewriting Systems (LCFRSs) ... in the right -hand side of a production is called the rank of G. As an example, context- free grammars are LCFRSs with f = 1 and r given by the maximum length of a production right -hand side. Tree ... 2010. c 2010 Association for Computational Linguistics Optimal rank reduction for Linear Context -Free Rewriting Systems with Fan-Out Two Benot Sagot INRIA & Universit´e Paris 7 Le Chesnay, France benoit.sagot@inria.fr Giorgio...

... properties that were common to various conswained grammatical systems, and defined a class of such systems called Linear Context -Free Rewriting Systems (LCFRS's). LCFRS's are constrained ... I. This system is called L/near Indexed Grammars because it can be viewed as a 279 COMBINATORY CATEGORIAL GRAMMARS: GENERATIVE POWER AND RELATIONSHIP TO LINEAR CONTEXT -FREE REWRITING SYSTEMS" ... that always formed the tree set of a context -free grammar. Formalisms that share these prop- erties were called Linear Context -Free Rewriting Systems ('LCFRS's) [14]. On the basis...

... IMS system implements three kinds of feature types and uses a linear kernel SVM as the classifier. Our evaluation on English lexical- sample tasks proves the strength of our system. With this system, ... 2010 System Demonstrations, pages 78–83, Uppsala, Sweden, 13 July 2010. c 2010 Association for Computational Linguistics It Makes Sense: A Wide-Coverage Word Sense Disambiguation System for Free ... baselines. However, developing such a system requires much effort. As a result, very few open source WSD systems are publicly available – the only other publicly available WSD system that we are aware of...

... synchronous context -free grammars. Computational Linguistics, 35(4):559–595. Laura Kallmeyer and Wolfgang Maier. 2010. Data- driven parsing with probabilistic linear context -free rewriting systems. In ... head- driven parsing strategy for Linear Context- Free Rewriting System productions. A head- driven strategy must begin with a specified righthand-side nonterminal (the head) and add the remaining ... parsing. 1 Introduction Linear Context -Free Rewriting Systems (LCFRSs) (Vijay-Shankar et al., 1987) constitute a very general grammatical formalism which subsumes context- free grammars (CFGs) and tree...

... development of formal systems for modeling the syntax of nat- ural language. There has been a considerable in- terest in rewriting systems that enlarge the generat- ive power of context -free grammars, ... in the right -hand side of a production is called the rank of G. As an example, context- free grammars are LCFRSs with f = 1 and r given by the maximum length of a production right -hand side. Tree ... write [i] as a shorthand for [1, i]. For an alphabet V , we write V ∗ for the set of all (fi- nite) strings over V . As already mentioned in Section 1, linear context -free rewriting systems generate...