... collection, and processed into buffy coats the same day as receipt for longterm storage at -80degC Our concern was the effect of longterm storage on total yield and fragmentation of DNA resulting from ... possible future problems with genome wide genotyping assays Conclusions The results of this pilot study demonstrate that buffy coats can be used as a longterm clinical trial or biobank specimen ... Buffy coat specimens remain viable as a DNA source for highly multiplexed genome -wide genetic tests after longterm storage Journal of Translational Medicine 2011 9:91 ...
... collection, and processed into buffy coats the same day as receipt for longterm storage at -80degC Our concern was the effect of longterm storage on total yield and fragmentation of DNA resulting from ... possible future problems with genome wide genotyping assays Conclusions The results of this pilot study demonstrate that buffy coats can be used as a longterm clinical trial or biobank specimen ... Buffy coat specimens remain viable as a DNA source for highly multiplexed genome -wide genetic tests after longterm storage Journal of Translational Medicine 2011 9:91 ...
... doi:10.1186/1477-7819-9-96 Cite this article as: Todoroki et al.: Long- term survivor of relapsed MFH on the thigh treated with autologous formalin -fixed tumor vaccine (AFTV) combined with limb-sparing surgery ... already been reported [5] We used a mixture of both paraffin-embedded primary tumor and formalinfixed secondary tumor as the vaccine In this way, we aimed to include the original tumor antigens ... Yamamoto T, Muroi A, Takano S, Enomoto T, Matsumura A, Ohno T: Clinical trial of autologous formalin -fixed tumor vaccine for glioblastoma multiforme patients Cancer Sci 2007, 98:1226-1233 Hafner J,...
... impact on long- term unemployment That is, the institutions affect long- term unemployment but have no direct impact on the weights To disentangle this direct effect from the effect via long- term unemployment ... Following the preferred OECD terminology, I will define as long- term unemployed those individuals in the labor force who have been out of work for one year or longer Short -term unemployed will be those ... matter in the determination of prices and wages, and that a smaller weight ought to be given to the long- term unemployed The results also show that in those countries where long- term unemployment...
... expected to significantly prolong healing achievable with other topical wound treatments [17] A complicated surgical wound is a wound likely to take significantly longer to heal than a similar ... Bacteroides species, Corynebacteria, and Mycoplasma [48] Psychoemotional aftermath With respect to the tsunami's psychoemotional aftermath, the full impact of the wave on the mental health of the survivors ... mental health is fairly new and only few data exist on what interventions may encounter short and longterm psychological problems One reason why valid epidemiological data are not yet sufficiently...
... mainly of secretory IgA (sIgA) [19] This study is the first, to our knowledge, to describe the longterm persistence of IgE anti-Influenza virus antibodies in serum of IgE positive and negative ... BioRad, Hercules, CA) Cytokine determination Serum cytokines [Interleukin-2 (IL-2), Interferon gamma (IFN-γ), Interleukin-4 (IL-4), Interluekin-10 (IL-10)] were determined by sandwich ELISA (Biosource, ... protocol Statistical Analysis Cytokine determinations from vaccinated and non vaccinated subjects were compared on each variable Significance between variables was determined using student’s t-tests...
... although epigenetic factors must be taken into account [7] The aim of the present study was to 95 determine the impact of CYP3A5*1 and CYP3A5*3 genotype on the kinetics of tacrolimus in renal transplant ... 1min at 55οC (primer annealing), 1min at 72οC (primer extension) and finally, 7min at 72οC (final elongation) The PCR product was analyzed on a 2% agarose/Tris-borate EDTA gel with ethidium bromide ... Taking into account that linear relationships, normal distribution of the dependent variables and fixed effects exist, the effects of between- and within-subjects factors as well as the interaction...
... study on longterm electrophysiological outcome and its impact on the QoL in a large patient group Study cohort: The number of patients lost in longterm follow up is consistent with long- term surveys ... is available concerning the development of QoL in the long- term The published literature mainly deals with the electrophysiological longterm results of RF ablation So far, QoL before and after ... ablation procedure as well as the primary and long- term electrophysiological success rates are in line with the literature (1-6, 9, 10) Quality of life in longterm follow-up: Prior to interventional...
... Ratio of long- termdebt (DLTT) minus debt maturing in and years (DD2þDD3) to total debt Total debt is defined as debt in current liabilities (DLC) plus long- termdebt (DLTT) Ratio of long- termdebt ... corporate debt arises because firms act as macro liquidity providers, issuing more longterm debt when the government issues more short -term debt and vice versa In Column we add the long- termgovernment ... information on the debt maturity structure as Compustat, and we can observe only the amount of shortterm and long- termdebt We calculate the ratio of longterm debt to total debt as a proxy for debt maturity...
... Domestic Waste in Landfill Sites," JWPCF 57(1), 30~38 3.Krug, M N., Ham, R K., (1997) "Analysis of Longterm Leachate Characteristics," SARDINIA 97, Sixth International Landfill Symposium, 117~131 4.Environmental ... containing the nitrogen of 1,700~2,200 mg/L(Fig 2) from to 12 m3/day Recycle ratios were kept at 600% In terms of effluent nitrogen concentration in each flow rate, T-N showed stable at 400 mg/L and NH4+-N...
... the long- term trend analysis of the water quality stated below This is because if a clear trend of precipitation is recognized, long- term trends in water quality will be affected by the long- term ... and Cossarini, G (2004) Long- term changes of inorganic nutrients in the Lagoon of Venice (Italy)., J Marine Sys., 51(1-4), 179-189 Parr, L.B and Mason, C.F (2003) Long- term trends in water quality ... was - 303 - Journal of Water and Environment Technology, Vol 7, No 4, 2009 reported by a long- term nationwide surveillance (Obara and Nakai, 2004) Furthermore, the phosphorus content of much of...
... contribute to the enhanced phenol removal in the longterm CONCLUSIONS We carried out experiments on phenol-contaminated water treatment over the longterm by using an S polyrrhiza–pond water bacteria ... electrophoresis (DGGE) We determined how the phenol-removal ability of the S polyrrhiza–bacteria association and the S polyrrhiza-associated bacterial community change during long- term treatment of phenol-contaminated ... and C23O gene on its root surfaces and the diversity of these bacteria can be maintained in the longterm under natural conditions and under conditions of phenol contamination - 244 - 0.8 0.6 0.4...
... of expectancy and valence of the compensation system Long- term Incentives Long- term incentives (LTI) are rewards linked to a firm’s long- term growth as well as employee retention (Rousseau & ... 001) and long- term incentives (p < 0001) Because of the restrictions on long- term incentives, Hypothesis predicted that the effect of bonuses would be stronger than the effect of long- term incentives ... Heskett, 1991) A problem with long- term incentives, though, is the extent to which employees feel their performance is connected to the level of reward Because long- term incentives are based on...